AIM:To investigate the effect of Toll-like receptor 9(TLR-9)gene rs5743836(1237 T/C)single nucleotide polymorphism on recurrent infec-tion in patients with diabetic foot(DF).MEHTODS:A total of 128 DF patients admitted to our hospital from June 2020 to June 2022 were collected and di-vided into an infected group(n=53)and a non-in-fected group(n=75)according to the presence or absence of recurrent infection.The general data and TLR-9 gene polymorphism were compared be-tween the two groups.The genotypes and allele frequencies of TLR-9(1237 T/C)gene polymor-phism were detected and compared between the two groups.Logistic regression was used to analyze the correlation between TLR-9 gene 1237 T/C poly-morphism and recurrence of DF patients.RESULTS:There were significant differences between the two groups in invasive operation,course of antibiotic use and peripheral vascular disease of both lower limbs(P＜0.05).The wild type TT homozygote pro-duced two fragments of 108 bp and 27 bp in length,the variant CC homozygote produced three fragments of 60 bp,48 bp and 27 bp in length,and the heterozygous TC produced four fragments of 108 bp,60 bp,48 bp and 27 bp in length.The TLR-9(1237 T/C)gene identity was 100％.The genotype frequencies met the Hardy-Weinberg genetic equi-librium(P＞0.05).There were significant differences in CC gene frequency,TC gene frequency,TT gene frequency,C gene distribution and T gene distribu-tion between the two groups(P＜0.05).Logistic re-gression analysis showed that TLR-9 CC genotype increased the risk of recurrent infection in DF pa-tients in the co-dominant model(OR=5.357,95％CI:1.901-15.100).After adjusting for sex,age and smoking(OR=5.341,95％CI:1.874-15.015,P＜0.01),the C allele significantly increased the risk of recur-rent infection in DF patients(OR=2.328,95％CI:1.874-15.015,P＜0.01).1.078-5.936),and the differ-ence was statistically significant(P＜0.01).The TT genotype and CC+TC genotype of TLR-9 were not significantly associated with smoking history,Wag-ner grade,peripheral neuropathy,retinopathy,hy-pertension,osteoporosis and lower extremity arte-riosclerosis obliterans in DF patients(P＞0.05).There were significant differences in the course of DM,DF,levels of HbAlc,LDL-C,CRP and PCT be-tween patients with CC genotype and patients with TT+TC genotype(P＜0.05).CC genotype,DM dura-tion ≥9 years,DF duration ≥5 months,HbAlc＜5.00％,LDL-C≥3.03 mmol/L,CRP≥23.25 mg/mL,PCT≥0.87 ng/mL were risk factors for recurrent infec-tion in DF patients(P＜0.05).HbAlc,LDL-C,CRP and PCT all showed interaction with TLR-9(1237 T/C)gene.The risk of recurrent infection in DF patients with abnormal HbAlc,LDL-C,CRP and PCT levels and TLR-9(1237 T/C)gene polymorphism interac-tion combination was 2.659 times higher than that in DF patients without the above combination,and the difference was statistically significant(P＜0.05).CONCLUSION:CC genotype and C allele of TLR-9 gene 1237 T/C are independent risk factors for re-current infection in DF patients.

AIM:To investigate the effect of Toll-like receptor 9(TLR-9)gene rs5743836(1237 T/C)single nucleotide polymorphism on recurrent infec-tion in patients with diabetic foot(DF).MEHTODS:A total of 128 DF patients admitted to our hospital from June 2020 to June 2022 were collected and di-vided into an infected group(n=53)and a non-in-fected group(n=75)according to the presence or absence of recurrent infection.The general data and TLR-9 gene polymorphism were compared be-tween the two groups.The genotypes and allele frequencies of TLR-9(1237 T/C)gene polymor-phism were detected and compared between the two groups.Logistic regression was used to analyze the correlation between TLR-9 gene 1237 T/C poly-morphism and recurrence of DF patients.RESULTS:There were significant differences between the two groups in invasive operation,course of antibiotic use and peripheral vascular disease of both lower limbs(P＜0.05).The wild type TT homozygote pro-duced two fragments of 108 bp and 27 bp in length,the variant CC homozygote produced three fragments of 60 bp,48 bp and 27 bp in length,and the heterozygous TC produced four fragments of 108 bp,60 bp,48 bp and 27 bp in length.The TLR-9(1237 T/C)gene identity was 100％.The genotype frequencies met the Hardy-Weinberg genetic equi-librium(P＞0.05).There were significant differences in CC gene frequency,TC gene frequency,TT gene frequency,C gene distribution and T gene distribu-tion between the two groups(P＜0.05).Logistic re-gression analysis showed that TLR-9 CC genotype increased the risk of recurrent infection in DF pa-tients in the co-dominant model(OR=5.357,95％CI:1.901-15.100).After adjusting for sex,age and smoking(OR=5.341,95％CI:1.874-15.015,P＜0.01),the C allele significantly increased the risk of recur-rent infection in DF patients(OR=2.328,95％CI:1.874-15.015,P＜0.01).1.078-5.936),and the differ-ence was statistically significant(P＜0.01).The TT genotype and CC+TC genotype of TLR-9 were not significantly associated with smoking history,Wag-ner grade,peripheral neuropathy,retinopathy,hy-pertension,osteoporosis and lower extremity arte-riosclerosis obliterans in DF patients(P＞0.05).There were significant differences in the course of DM,DF,levels of HbAlc,LDL-C,CRP and PCT be-tween patients with CC genotype and patients with TT+TC genotype(P＜0.05).CC genotype,DM dura-tion ≥9 years,DF duration ≥5 months,HbAlc＜5.00％,LDL-C≥3.03 mmol/L,CRP≥23.25 mg/mL,PCT≥0.87 ng/mL were risk factors for recurrent infec-tion in DF patients(P＜0.05).HbAlc,LDL-C,CRP and PCT all showed interaction with TLR-9(1237 T/C)gene.The risk of recurrent infection in DF patients with abnormal HbAlc,LDL-C,CRP and PCT levels and TLR-9(1237 T/C)gene polymorphism interac-tion combination was 2.659 times higher than that in DF patients without the above combination,and the difference was statistically significant(P＜0.05).CONCLUSION:CC genotype and C allele of TLR-9 gene 1237 T/C are independent risk factors for re-current infection in DF patients.

Objective To explore unexplained syncope (UPS) morbidity of elementary and middle school students in Changsha city.Methods Four thousand two hundred and fifteen students aged 5 to 18 years from six fulltime elementary or middle schools were randomly selected and the survey was conducted by giving them 4 215 copies of questionnaires and they were categorized into 3 groups:key school group(n =1 062),normal school group(n =1 310) and peasant laborer dependent's school group (n =1 349).Three thousand seven hundred and twenty-one copies of returning questionnaires (88.28％) were collected.Results 1.Morbidity:among 3 721 students there were 738 students(19.83％) who had once or more symptom of UPS.And the morbidity of middle school students was higher than elementary school students (28.87％ vs 7.96％,x2 =252.11,P ＜ 0.01).The morbidity of key school students was higher than normal school and peasant laborer dependent's school students (25.99％ vs 16.34％ vs 18.38％,x2 =40.93,P ＜ 0.01).2.Onset age:the onset age of UPS was 6-18 (14.23 ± 2.70) years old.3.Gender difference:for the middle school group,the morbidity of female students was higher than male students(31.34％ vs 26.49％,x2 =6.06,P ＜ 0.05).But for the elementary school group (male 9.04％ vs female 6.68 ％,x2 =2.85,P ＞ 0.05) and the total secondary school students (male 18.67％ vs female 21.12％,x2 =3.51,P ＞ 0.05),there was no significant difference between genders.Conclusions The UPS morbidity of students in Changsha is 19.83％.There are differences in age,school and gender.

The root of Peucedanum harry-smithii var. subglabrum was extracted with methanol, then separated with solvents at different polarity into four fractions: aqueous (H2O), ethyl acetate (AcOEt), chloroform (CHCl3) and petroleum ether (DAB-6). From AcOEt psoralen, bargapten, xanthotoxin, marmesin, umbelliferone, scopoletin, (+/-) peuformosin, Pd-I b, (+/-) selinidin, praeruptorin D were isolated by column chromatography on silica gel, using petroleum ether/ethyl acetate as eluent. The structures of the coumarins were identified by 1H-NMR and 13C-NMR.
Apiaceae ; chemistry ; Coumarins ; chemistry ; isolation & purification ; Magnetic Resonance Spectroscopy ; methods ; Plant Roots ; chemistry ; Umbelliferones ; chemistry