Background: Plants of the genus Achillea are widely used in both traditional and modern medicine and are known to be rich in biologically active secondary metabolites. Numerous phytochemical and histochemical studies have been con ducted on Achillea millefolium L.; however, Achillea asiatica Serg., which is widely distributed in Mongolia, remains comparatively underexplored. Therefore, investigating the distribution of biologically active compounds, the content of polyphenolic compounds, and the optimal extraction conditions of this species is of significant scientific and practical importance. Aim: The aim of this study was to identify biologically active compounds in Achillea asiatica Serg. using histochemical methods, to determine the content of polyphenolic compounds and optimal extraction conditions, and to evaluate the total and internal absorption coefficients of the plant raw material. Materials and Methods: The aerial parts of Achillea asiatica Serg. were collected in August 2025 from Erdene soum, Tuv province, Mongolia. Histochemical analysis was performed on transverse stem sections and flower petals using potassium dichromate, ferric chloride (III), methylene blue, Lugol’s iodine, and Dragendorff’s reagent, followed by ob servation under a light microscope. The total polyphenolic content was determined using the Folin–Ciocalteu method and expressed as gallic acid equivalents. Extraction experiments were conducted using powdered plant material sieved to par ticle sizes of 1, 2, and 3 mm and extracted with 20–80% ethanol. In addition, the total and internal absorption coefficients of the plant material were determined according to standard procedures. Results: Histochemical analysis revealed the presence of essential oils, polyphenolic compounds (tannins and flavo noids), and starch, while alkaloids were not detected. Polyphenolic compounds were observed in both stems and flow ers, with more pronounced accumulation in the vascular tissues. The highest total polyphenolic content (0.421%) was obtained from the 1 mm particle-size fraction extracted with 70–80% ethanol. Using 60% ethanol, the total absorption coefficient of the plant material was determined to be 5.74, and the internal absorption coefficient was 1.885. Conclution Achillea asiatica Serg. is rich in polyphenolic compounds, and their anatomical distribution is closely as sociated with the plant’s protective and biological functions. The use of 1 mm particle-size material and 70–80% ethanol was identified as the most suitable condition for extracting polyphenolic compounds. These findings provide a scientific basis for the standardization of Achillea asiatica Serg. as a medicinal and phytopharmaceutical raw material and support its further pharmacognostic and phytochemical investigation.

Background: The Shimshin-6 formulation, composed of Rheum undulatum L., Hippophae rhamnoides L., Zingiber officinale Roscoe, Saussurea lappa C.B. Clarke, Sal ammoniacum, and Tronae veneni, has traditionally been used in the management of gynecological disorders such as amenorrhea, abnormal blood clot formation, and pelvic pain. It has also been widely applied in postpartum care to promote uterine contractility and to prevent complications, including hemorrhage and inflammation. However, its therapeutic potential in postpartum uterine involution has not been scientifically validated. Aim: To investigate the effects of the Shimshin-6 herbal formulation on postpartum uterine involution in rats Materials and Methods: This experimental study was conducted at the Research Center of the Institute of Traditional Medicine and Technology, following the methodology described by Zheng ZH et al. (2019). Postpartum rats were randomly assigned to four groups and received daily treatments for five consecutive days with either Shimshin-6 tablets at doses of 180 mg/kg and 90 mg/kg, Shimshin-6 decoction at 163 mg/kg, or physiological saline as a control. Serum concentrations of estrogen, progesterone, TNF-α, TGF-β1, and TGF-β3 were measured using commercially available ELISA kits (Shanghai MLBIO Biotechnology Co. Ltd, China). Following ethical euthanasia, uterine tissues were collected for histological examination, which included evaluation of uterine weight, endometrial thickness, myometrial structure, and glandular morphology. Results: Shimshin-6 treatment significantly reduced the uterine index and serum estrogen levels compared with the control group, while no significant difference was observed in progesterone concentration. The treatment also decreased tumor necrosis factor-α (TNF-α) levels and modulated growth factors in postpartum rats. Histological analysis further revealed that Shimshin-6 promoted uterine recovery, despite the presence of inflammatory cell infiltration and hemorrhagic changes in the uterine wall. Conclusion Shimshin-6 preparation exerts a promotive effect on postpartum uterine involution in rats.

Background: Musculoskeletal disorders account for 23.1-47.1% of occupational diseases in several countries. Studies have shown that operators of heavy machinery, including tractors and dump trucks, are twice as likely to experience lower back pain compared to workers not exposed to whole-body vibration. Furthermore, research has indicated that acute exposure to vibration can cause vasoconstriction and vascular inflammation. However, limited research has explored the relationship between lower back pain and specific biomarkers, highlighting the need for this study. Aim: This study aimed to compare lower back pain prevalence and muscle inflammation biomarkers among heavy machinery operators. Materials and Methods: A purposive sampling method was used to recruit 15 male participants aged 25-35 years who had worked as heavy machinery drivers for no more than three years. Inclusion criteria were: no alcohol consumption within 24 hours prior to sampling, body mass index (BMI) between 18.5-28.9 kg/m², no prior diagnosis of musculoskeletal disorders, and absence of infectious or non-infectious diseases during the study period. Blood samples were analyzed for Interleukin-6 (IL-6) and Tumor Necrosis Factor-alpha (TNF-α) levels using enzyme-linked immunosorbent assay (ELISA). Results: The mean BMI of participants was 25.89±3.23 kg/m². Over half (53.3%, n=8) exceeded the exposure limit for whole-body vibration. Low back pain was reported by 13 participants (86.7%) over the past six months and by 12 participants (80%) over the past seven days. TNF-α levels did not differ significantly between groups based on low back pain status or vibration exposure. However, IL-6 levels showed a significant increase 24 hours after whole-body vibration exposure (p=0.027). Conclusion Lower back pain was highly prevalent among participants exposed to whole-body vibration. Furthermore, IL-6 levels were elevated among participants reporting lower back pain, regardless of vibration exposure levels.

Background: In traditional medicine, the Shimshin-6 formulation, which consists of Rheum undulatum L., Hippophae rhamnoides L., Zingiber officinalie Roscoe, Saussurea Lappa C.B.Clark, Sal ammoniacum, Tronae veneni, is recommended for women experiencing menstrual retention disorders. In recent years, Shimshin-6 has been widely used to promote postpartum uterine involution for women and our study aimed to evaluate and determine the acute and chronic toxicity effects of Shimshin-6. Aim: To evaluate and substantiate the acute and chronic toxicity effects of Shimshin-6. Materials and Methods: The acute toxicity of Shimshin-6 was evaluated using the rapid method described by V.B. Prozorovsky (1978) by administering intraperitoneal injections of the medicinal extract in white mice to determine the lethal dose. The active dose was determined following the methodology of I.P. Zapadnyuk (1983). Chronic toxicity was evaluated in Wistar rats according to the OECD 407 (2008) guidelines. The test animals were administered Shimshin-6 in tablet form (90 mg/kg and 180 mg/kg) and decoction form (tang) (162 mg/kg) daily for 60 days. At the end of the experiment, biochemical and complete blood analyses were conducted, along with histopathological examination of major organs. The study was conducted with ethical approval granted by the Ethics Committee of the Mongolian National University of Medical Sciences (MNUMS) on October 25, 2024. Results: The LD50 for Shimshin-6 tablets was 4.47 (3.39–5.1) g/kg, indicating low acute toxicity based on the K.K. Sidorov classification. The LD50 for the decoction form was 8.1 (7.1–9.4) g/kg, suggesting it is non-toxic. Regarding chronic toxicity, platelet count was significantly reduced compared to the healthy control group: Shimshin-6 tablet group: 46% reduction at 90 mg/kg and 29.7% reduction at 180 mg/kg. Shimshin-6 decoction group: 60.5% reduction at 162 mg/ kg. Additionally, hemoglobin levels in the decoction group (162 mg/kg) decreased by 15.7% (p<0.05). Biochemical analysis showed a 36.3% reduction in total cholesterol (LDL-C) levels in the tablet group (180 mg/kg) and decoction group (162 mg/kg) compared to the control (p<0.05). Conclusion Shimshin-6 tablets showed low acute toxicity in experimental mice. However, long-term administration may lead to a reduction in platelet count.

Introduction: We classify peritonitis as end-stage if it lasts for more than 72 hours or more than three days. At this point, the pleural effusion of the posterior abdominal wall, the pleural layer of the gastrointestinal tract, and the dimples of the esophagus are all scattered with pus. During the first operation, it is very difficult to completely cleanse these abscesses. After the operation, pus will collect in the abdomen and abscesses will form, which will require another operation. If this postoperative complication is not diagnosed in time and operated again (relaparotomy), many other complications can occur and the risk of death is high. 48-hour relaparotomy mortality is higher than early surgery (21.8% -76.8%). Necrotic pancreatitis is chronic peritonitis (an abscess of the lower extremities) in which only non-pancreatic adipose tissue, sebum glands, pericardial effusions, pericardial effusions, and kidney adipose tissue become necrotic. Purpose: Endoscopic surveillance for chronic pleurisy with pancreatic necrosis Objectives:
1. Endoscopic monitoring of the postoperative course of pancreatic necrosis.
2. Calculate the results of washing and cleaning using binoculars. Method: Patients with advanced peritoneal inflammation and necrotizing pancreatitis should be selected for reoperation. After removing the dead pancreatic tissue (necrosectomy), all layers of the abdomen are temporarily closed. A 6 mm short tube with surgical rubber is cut into the small pancreas, inserted 2 cm deep into the standard abdomen and sutured to the skin. Or use a silicone tube 4 - 5 cm long. Result: The study was carried out on 56 patients in 2016-2020. The mean age was 50 (89%) for men, 6 (11%) for women, and 47.5 ± 8.6. Conclusion
1. Endoscopic follow-up showed 19 (76%) persistent postoperative peritonitis and re-clearance, and 6 (24%) patients were not diagnosed with persistent peritonitis.
2. 25 (50%) cases of persistent peritonitis after surgery were washed 1-3 times. This method has proven to be a safe and easy procedure and can be used in any urban or rural hospital.

The present study was conducted to assess relationship between drinking water fluoride levels and dental caries among 12 years old school children of 2 districts of Ulaanbaatar city, Mongolia. A cross-sectional analytical study was conducted on 533 school children aged 12 years, selected from 6 schools of 2 districts of Ulaanbaatar city. 533 children were divided into 2 groups according to the fluoride concentration of the waters. The all children were examined oral examination, dental caries was assessed by the DMF-T index. The result of the present study revealed that the caries prevalence in the study population was about 68,9%, and mean DMF-T was 3.05. Water fluoride concentration was highest in Khan-Uul district with 0.622ppm. There was highest prevalence of caries in children who consume water from filtration system in both districts.

The present study was conducted to assess relationship between drinking water fluoride levels and dental caries among 12 years old school children of 2 districts of Ulaanbaatar city, Mongolia.A cross-sectional analytical study was conducted on 533 school children aged 12 years, selected from 6 schools of 2 districts of Ulaanbaatar city. 533 children were divided into 2 groups according to the fluoride concentration of the waters. The all children were examined oral examination, dental caries was assessed by the DMF-T index.The result of the present study revealed that the caries prevalence in the study population was about 68,9%, and mean DMF-T was 3.05. Water fluoride concentration was highest in Khan-Uul district with 0.622ppm.There was highest prevalence of caries in children who consume water from filtration system in both districts.

There were currently diagnosed by totally 177 cases with HIV in our country. The 82.3% of those were male and 72.9% of them were men sex with man. Sexual minority group especially men who intercourse with male are highly risk to HIV infection. By the study of 2011, which aimedto assess risk to exposure HIV infection among sexual minority group, 56.2% of all participants answered as had depression symptoms and 12.8% of them seen to psychologist and health workers. In addition, 2% of them used by injection of drug for last year, and 60.6% harmfully drank.The study was done by quantitative and qualitative methods and used specific designed questionnaire for sexual minority with HIV infection. Totally 26 consumers participated in our survey. Before the starting of the study, we introduced inform consent to all participants and if they agreed to participate, we coded research cards and collected the information.All participants were answered alcohol drink, and 46.1% of them determined with heavy drinking or harmful consumption by the AUDIT versus 7.8% were alcohol dependence. 65.3% oftotal participants used as smoke, 5 cases used cannabis; one of them has been used within last twelve months. In addition, 23.1% of all participants had anxiety and 15.3% had mild depression symptoms. Of the total 26 cases, 7 cases had suicide thinking related with sexual orientationand 2 cases had suicide thinking related with HIV infection. Those cases answered often feeling hopeless and helpless.In sexual minority group with HIV, alcohol and tobacco consumption was high, drug abuse was low. 15.3-23.1% of all participants had depression and anxiety symptoms. Suicide thinking has been occurred for those cases it means risk to suicide commitment.

IntroductionThere are an estimated 246 million people with diabetes mellitus globally and this figure is predictedto rise to 380 million by 2025, with the most rapid growth in developing countries, among the workingage group of the population.Now in Mongolia, the more than half of population live in cities and settlements, following thisurbanization the population`s diet and lifestyle has been changed to more western style and morepeople affected by diabetes mellitus.Diabetic retinopathy is the most common chronic and devastating complication of diabetes whichleads to visual impairment and blindness. Diabetic retinopathy develops in nearly all persons withtype 1 diabetes and in more than 77% those with type 2 who survive over 20 years with disease. Thecurrent estimates of the prevalence of diabetic retinopathy vary in different countries. The WHO hasestimated that diabetic retinopathy is responsible for 4.8% of 37 million cases of blindness throughoutthe world.Significant independent predictors of proliferative diabetic retinopathy determined by multivariableanalysis were fasting plasma glucose level, duration diabetes, plasma cholesterol, systolic bloodpressure and therapeutic regimen.In recent years a number of randomized clinical trials have shown that interventions to improvemetabolic control, careful monitoring and treatment reduce late diabetic complications.GoalTo establish the prevalence, severity and risk factors of diabetic retinopathy type 2 diabetic patientsin Ulaanbaatar.Materials and MethodsThe study conducted by cross sectional study. Randomly selected 235 patients type 2 diabetic patientsfrom Bayanzurh districts of city Ulaanbaatar. Participants provided a detailed medical and personalhistory, underwent an ocular examination including funduscopy. Fasting blood glucose-FBG, totalcholesterols, triglyceride, HDL were determined by methods of laboratory in venous plasma.ResultsThe prevalence of diabetic retinopathy among people with type 2 diabetes was 37.4%. The prevalencenon-proliferative diabetic retinopathy was 17% (40), pre-proliferative diabetic retinopathy was 8.1%(19), and of proliferative diabetic retinopathy was 12.3 %( 29).The mean age of participants with diabetic retinopathy was 57.4 years (range 26-79). The meanage of participants with non-diabetic retinopathy was 53.6 years (range 26-76). The prevalence of diabetic retinopathy did not vary significantly with age. Retinopathy was positively associated with alonger reported duration of diabetes and with higher fractions of blood glucose (p<0.05).Progression of diabetic retinopathy positively associated with high level fasting blood glucose (6.2mmol/l), total cholesterol (4.5 mmol/l), systolic pressure (130 mm Hg) diastolic pressure (90 mm Hg),triglyceride (2.2 mmol.l) and low level HDL (1.1 mmol/l).ConclusionRisk factors for diabetic retinopathy were found to be high level of blood glucose, longer duration ofdiabetes. Diabetes poor control was significantly associated with progression of diabetic retinopathy.

BackgroundEarly detection of minor strokes and their treatment that aim to prevent from complications into severe strokes is a process of secondary prevention. There is a need to extensively use image diagnostics (CT, MRI) because signs are obscure, at times without focal neurological sign but can have special mental or psychological syndromes. The start of minor stroke studies in Mongolia will enable further deepening of these studies in future and give an impetus to identification of theoreticaland practical aspects together with further improvement of diagnostics, treatment and prevention of minor strokes.GoalTo develop and introduce the diagnostic criteria of ischemic and hemorrhagic minor strokes in accordance to the concepts of minor strokes and to treat minor stokes in order to prevent complications into severe strokes.Materials and MethodCurrently there are no globally accepted diagnostic criteria for minor stroke. We support the 1981 WHO criteria of minor strokes as strokes neurological signs of which disappear in relatively short period of time. There is a general notion that it should mean all light forms of stroke other than severe strokes. In cases of neurological signs of a minor stroke, complete recovery and elimination of the symptoms take up to 3 weeks. Most scholars tend to consider ischemic lacunar strokes (arising from occlusion of arteriole vessels deep in the brain and with size of 0.5-20 mm) as minor strokes. We maintained the concept that characteristic features of these strokes are their limited focal areas and the following neurological symptoms: pure motor, pure sensory, light ataxia, etc. We also duly considered a suggestion (D. German, L. G. Koshchug et al, 2008 ) to define minor hemorrhagic strokes as strokes with diameter less than 2 cm and blood volume less than 5 cm3.We identified 60 patients with minor strokes, involved in monitoring using special research template (with a term of at least 1.5years) and involved in pathogenesis treatment. In the treatment, we maintained a principle of differential diagnosis of ischemic stroke symptoms. Specifically, we differentiated the following: signs related to an atherotromb, cardio-embolic, lacunar, hemodynamic, hemorheologic pathogenesis. To verify the diagnoses, we used MRT and CT image tests. We executed paraclinic tests in order to identify risk factors: Doppler-duplex-sonography, brain angiography, blood lipid fraction, ECG, EchoCG, heart Holter, blood hemorheology test, and identified the most affecting factors (hereditary factors, excess weight, smoking etc).Results: Our study identified the following clinical forms: lacunar stroke, non-lacunar minor stroke, and hemorrhagic minor stroke. Among the minor strokes, the lacunar stroke dominates (48%), the nonlacunar stroke is the next (27.7%), and the hemorrhagic was found to be the least common 25%. From among a host of risk factors, arterial hypertension is dominant (86%) either alone or in combination with such other diseases as diabetes, atherosclerosis etc. Diabetes occurrence was 5 cases (8,3%) which is fewer than in some foreign studies.The clinic of minor stroke also varies. The strength and expression of their symptoms compared with those of severe strokes are unique in the following:- Relatively lighter and recover faster as a result of treatment even in acute forms,- Some are without specific clinical signs (“silent stroke”).- Some minor strokes have micro focal signs, for example, “pure motor”, pure sensory, ataxia etc, in other words, the signs are limited.- In cases of lacunar strokes, predominantly deep brain arterioles are damaged.- Whereas in non-lacun strokes, embolic, ateroma, thrombotic mechanisms are predominant suchas distal branches of big artery. - In cases of hemorrhagic minor strokes, arteriopathy distortions occur not only in depth of brain but also in any small lobar vessels of brain.- Focal lesions have some variations by their pathological locations and minor stroke signs.In non-lacunar strokes (25%), the focal damages predominantly occur in branches of large intra/extra cranial arteries. In cases of lacunars strokes, the focal lesion is not in branches of large intracranial vessels, but is predominantly in basal ganglia, deep white matter, thalamus, pons and in area of deep penetrating arterial vessels. However, focal infarcts in cerebella may occur in any form of minor strokes.ConclusionAccording our study there were identified 3 subtypes of minor stroke. The finding is that lacunars and hemorrhagic minor strokes are more likely to give grounds to severe strokes. From this, it can be concluded that there are specific factors in the population of Mongolia to affect the genesis of minor strokes, namely, arterial hypertension which is directly related with these forms of minor strokes. We appropriate the WHO criteria of minor stroke that is neurological signs of a minor stroke, complete recovery and elimination of the symptoms take up to 3 weeks. In treatment of minor stroke, we suggest that minor strokes should be treating by pathogenetic therapy. Namely, antihypertensive therapy for lacunar infarction, anti-aggregation therapy for nonlacunar infarction and haemostatic and antihypertensive therapy for hemorrhagic minor stroke.