Background: Mast cell activation and the release of inflammatory mediators play central roles in the pathogenesis of urticaria. Luteolin possesses anti-inflammatory and anti-allergic properties, whereas the luteolin-gadolinium complex (Lut-Gd) has demonstrated anti-inflammatory activity in macrophage models. However, the effects of Lut-Gd on mast cell activation remain unclear. Aim: To evaluate the effects of Lut-Gd on cell viability, cytotoxicity, and inflammatory mediator release in Compound 48/80 (C48/80)-stimulated HMC-1 cells. Materials and Methods: HMC-1 cells were stimulated with C48/80 and treated with either luteolin or Lut-Gd. Cell viability and membrane integrity were assessed using CCK-8 and lactate dehydrogenase (LDH) release assays, respectively. Histamine, IL-1β, IL-6, and TNF-α concentrations in culture supernatants were quantified by enzyme-linked immunosorbent assay (ELISA). Results: At the concentrations selected for subsequent experiments, C48/80 (10 μg/mL), luteolin (2.5 μg/mL), and Lut-Gd (10 μg/mL) did not induce significant cytotoxicity. C48/80 stimulation significantly increased the release of histamine, IL-1β, IL-6, and TNF-α from HMC-1 cells. Treatment with either luteolin or Lut-Gd significantly reduced the levels of these inflammatory mediators (p < 0.05). Conclusion Lut-Gd suppressed C48/80-induced release of histamine and inflammatory cytokines from HMC-1 cells without inducing significant cytotoxicity, supporting its potential anti-inflammatory activity in mast cell-mediated inflammation.

Appendiceal mucocele is an obstructivedilatation of the appendix caused byintraluminal accumulation of mucoid material.It is a rare disease. The incidence is 0.2%to 0.3% of all appendectomied specimens.The brush size to 3 cm are considered to besmall, up to 6 cm - average and more than9 cm - giantCases: the patient 50 years old, man,place of residence: the city of UB songinokhairkhan duureg. Specialists of the driver,in the year immediately 2015,02,02 wasoperated with the diagnosis of acuteappendicitis. Complaints on admission:pain on the right side of the abdomen, anagging pain when moving and walking,stomach rumbling. History of the disease:the pain started with 2015,01,31 at 8 pmfrom the bottom of the abdomen. tookhome chloramphenicol but not helpedso 2015,02,02 year at 9 o’clock enteredvia ambulance. Medical examination: thesatisfactory condition of the active position,the skin clean, moist, blood pressure160/110, pulse 98 beats per minute Spo298%. 10*7,7*5,5 см size /Fig.1-2/ , white /Fig. 3-4/ , smooth surface /Fig.1-4/, insideslimy gray mucinous /Fig. 5-6/ bag /Fig. 5/above listed items on a standard attached,aims to study the histology. The result ofthe following: Mucocele of the appendixwith hyalinized wall and with the additionof acute inflammation with microabscesses.