1.Role of caffeine and ethanol in modulating expression of Receptor Activator of Nuclear Factor κβ (RANK) and Osteoprotegerin (OPG) during orthodontic tooth movement: An in vivo study.
Ardiansyah S. PAWINRU ; Eka ERWANSYAH ; Eddy Heriyanto HABAR ; Abul FAUZI ; AMINULLAH ; Gita GAYATRI ; Yustisia PUSPITASARI ; Ita Purnama ALWI ; Andi Husnul HASANAH
Acta Medica Philippina 2026;60(8):115-122
BACKGROUND AND OBJECTIVES
Orthodontic tooth movement is driven by bone remodeling influenced by systemic factors, including caffeine and ethanol. This study aimed to investigate the effects of caffeine and ethanol on the expression of Receptor Activator of Nuclear Factor κβ (RANK) and Osteoprotegerin (OPG), key bone remodeling biomarkers, during orthodontic tooth movement.
METHODSA laboratory experimental study was conducted on 30 male Wistar rats divided into three groups: K1 (orthodontic force only), K2 (force + caffeine), and K3 (force + ethanol). Orthodontic force was applied using Ni-Ti coil springs. Caffeine and ethanol were administered orally daily. On days 7 and 14, maxillary tissues were collected and analyzed via immunohistochemistry for RANK and OPG expression. Data were analyzed using One-Way ANOVA and Independent Sample T-tests with significance at pRESULTS
Caffeine and ethanol administration increased RANK and OPG expression compared to controls; however, only the ethanol group showed a significant increase in RANK expression on day 14 (p = 0.044). OPG expression was significantly higher in treatment groups at both time points (pCONCLUSION
Caffeine and ethanol modulate bone remodeling marker expression during orthodontic force application, with ethanol significantly increasing RANK expression at later stages. Further studies are needed to clarify the clinical implications for orthodontic treatment.
Animals ; Tooth Movement Techniques ; Tooth Movement ; Osteoprotegerin ; Role ; Movement ; Ethanol ; Bone Remodeling ; Caffeine ; Immunohistochemistry
2.Role of caffeine and ethanol in modulating expression of Receptor Activator of Nuclear Factor κβ (RANK) and Osteoprotegerin (OPG) during orthodontic tooth movement: An in vivo study.
Ardiansyah S. PAWINRU ; Eka ERWANSYAH ; Eddy Heriyanto HABAR ; Abul FAUZI ; AMINULLAH ; Gita GAYATRI ; Yustisia PUSPITASARI ; Ita Purnama ALWI ; Andi Husnul HASANAH
Acta Medica Philippina 2026;60(8):115-122
BACKGROUND AND OBJECTIVES
Orthodontic tooth movement is driven by bone remodeling influenced by systemic factors, including caffeine and ethanol. This study aimed to investigate the effects of caffeine and ethanol on the expression of Receptor Activator of Nuclear Factor κβ (RANK) and Osteoprotegerin (OPG), key bone remodeling biomarkers, during orthodontic tooth movement.
METHODSA laboratory experimental study was conducted on 30 male Wistar rats divided into three groups: K1 (orthodontic force only), K2 (force + caffeine), and K3 (force + ethanol). Orthodontic force was applied using Ni-Ti coil springs. Caffeine and ethanol were administered orally daily. On days 7 and 14, maxillary tissues were collected and analyzed via immunohistochemistry for RANK and OPG expression. Data were analyzed using One-Way ANOVA and Independent Sample T-tests with significance at pRESULTS
Caffeine and ethanol administration increased RANK and OPG expression compared to controls; however, only the ethanol group showed a significant increase in RANK expression on day 14 (p = 0.044). OPG expression was significantly higher in treatment groups at both time points (pCONCLUSION
Caffeine and ethanol modulate bone remodeling marker expression during orthodontic force application, with ethanol significantly increasing RANK expression at later stages. Further studies are needed to clarify the clinical implications for orthodontic treatment.
Animals ; Tooth Movement Techniques ; Tooth Movement ; Osteoprotegerin ; Role ; Movement ; Ethanol ; Bone Remodeling ; Caffeine ; Immunohistochemistry
3.A comparison of lactobacillus acidophilus adhesion to metal and ceramic brackets with coated and uncoated nickel titanium orthodontic archwires: An in vitro study
Ardiansyah S. Pawinru ; Nasyrah Hidayati ; Eka Erwansyah ; Eddy Heriyanto Habar ; Baharuddin M. Ranggang ; Suhesti Suronoto
Acta Medica Philippina 2024;58(22):106-110
Background and Objective:
According to microbiological investigations, microorganisms, especially Lactobacillus strains, considerably increase after using fixed orthodontic appliances. One of the Lactobacilli bacteria found in the oral cavity is Lactobacillus acidophilus. The purpose of this study was to compare the adhesion of Lactobacillus acidophilus to metal and ceramic brackets with coated and uncoated nickel titanium (NiTi) orthodontic archwires.
Methods:
Forty () samples were divided into four groups for this in vitro study: 10 metal brackets with coated NiTi archwire, 10 metal brackets with uncoated NiTi archwire, 10 ceramic brackets with coated NiTi archwire, and 10 ceramic brackets with uncoated NiTi archwire. Elisa Reader was used to count the number of Lactobacillus acidophilus attachments, and the one-way ANOVA and Tukey HSD tests were used to analyze all results.
Results:
The results showed significant differences in the attachment of Lactobacillus Acidophilus between the ceramic bracket and coated NiTi archwire sample groups and the metal bracket and uncoated NiTi archwire sample groups (P= 0.01). The adherence of Lactobacillus acidophilus to the ceramic bracket and uncoated NiTi archwire group was higher than the metal bracket and coated NiTi archwire group, and the metal bracket and uncoated NiTi archwire group. The attachment of Lactobacillus acidophilus to the metal bracket and uncoated NiTi archwire groups was the lowest of all sample groups in this study.
Conclusion
The highest Lactobacillus acidophilus adherence was in the ceramic bracket with coated NiTi archwire group compared to the other three groups.
Lactobacillus acidophilus
;
Orthodontic Brackets
;
Orthodontic Wires
Result Analysis
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