Introduction: Anaemia remains a significant public health concern among adolescent girls in Indonesia. Weekly iron-folic acid supplementation is widely implemented; however, evidence from supervised school-based settings is limited. This study aimed to examine changes in haemoglobin levels following supervised weekly supplementation. Methods: A quasi-experimental study with repeated measurements was conducted among adolescent girls in one junior high school. Participants received weekly ferrous fumarate (60 mg iron and 400 µg folic acid) for 12 weeks under school supervision. Haemoglobin levels were measured at baseline and follow-up. Data were analysed using General Linear Model Repeated Measures (GLM-RM), with additional analysis of covariance (ANCOVA) to adjust for baseline differences. Results: A total of 47 participants completed the study. Haemoglobin levels increased in the intervention group (10.8 to 13.2 g/dL), while minimal change was observed in the comparison group (12.4 to 12.9 g/dL). GLM-RM showed a significant effect of time (p<0.001) and time–group interaction (p=0.001). However, after baseline adjustment using ANCOVA, the difference between groups was not statistically significant (p=0.296). Conclusion: Supervised weekly iron-folic acid supplementation was associated with improved haemoglobin levels over time. However, baseline differences may have influenced the observed effects. Strengthening school-based programmes through structured supervision and adherence monitoring may improve implementation. Further research using baseline-adjusted designs is needed to better estimate the true effect of supplementation.

Background: Conditioned medium is the medium obtained from certain cultured cells and contained secretome from the cells. The secretome, which can be in the form of growth factors, cytokines, exosomes, or other proteins secreted by the cells, can induce the differentiation of cells that still have pluripotent or multipotent properties. Objectives: This study examined the effects of conditioned medium derived from E17 rat brain cells on cells with pluripotent properties. Methods: The conditioned medium used in this study originated from E17 rat brain cells. The CM was used to induce the differentiation of primary colonies of mice blastocysts. Primary colonies were stained with alkaline phosphatase to analyze the pluripotency. The morphological changes in the colonies were examined, and the colonies were stained with GFAP and Neu-N markers on days two and seven after adding the conditioned medium. Results: The conditioned medium could differentiate the primary colony, beginning with the formation of embryoid-body-like structure; round GFAP positive cells were identified. Finally, neuron-like cells testing positive for Neu-N were observed on the seventh day after adding the conditioned medium. Conclusions Conditioned medium from different species, in this case, E17 rat brain cells, induced and promoted the differentiation of the primary colony from mice blastocysts into neuron-like cells. The addition of CM mediated neurite growth in the differentiation process.