Objective To observe the value of hemagglutinin occlusion of needle passage for preventing complications of pulmonary puncture biopsy.Methods Data of 155 patients with single pulmonary nodule who underwent CT-guided percutaneous lung puncture biopsy were retrospectively analyzed.The patients were divided into observation group(n=78,injected hemagglutinin when withdrawing the needle)and control group(n=77,directly withdrawn the needle).Chest CT examination was performed immediately after completion of the procedure.Pulmonary hemorrhage grades were evaluated,and the proportions of compressed lung area were measured and compared between groups.Results Puncture and sampling were successfully performed in all 155 patients,and definite pathological diagnose was acquired in 150 cases(150/155,96.77%).High-grade hemorrhage was detected in 20 cases(20/78,25.64%)of observation group and 38 cases(38/77,49.35%)of control group,while pneumothorax was noticed in 18 cases(18/78,23.08%)with lung compression area proportion of(0[0,0])in observation group,and in 31 cases(31/77,40.26%)with lung compression area proportion of 0[0,0.76%]in control group,respectively,in observation group were better than in control group(all P＜0.05).Conclusion Occlusion needle passage when withdrawing needle with hemagglutinin after pulmonary puncture biopsy could reduce the risk of hemorrhage and pneumothorax.