BACKGROUND:Polycaprolactone has been widely used in bone tissue engineering due to its excellent processing and degradation performance,but its poor hydrophilicity and mechanical strength cannot provide a good growth environment for osteoblasts.OBJECTIVE:To prepare polycaprolactone/nanodiamond-phospholipid composite materials,evaluate its biocompatibility and in vitro ability to promote bone differentiation.METHODS:Nanodiamond was modified using phospholipids,and polycaprolactone was used as the raw material.Polycaprolactone/nanodiamond-phospholipid composite materials with different mass ratios(0%,2.5%,7.5%,and 10%)were prepared by solution casting method.Polycaprolactone/nanodiamonds were used for comparison.The surface morphology,elemental composition,mechanical properties,and water contact angle of each group of materials were observed to select composite materials with better physical and chemical properties.MC3T3-E1 cells were inoculated onto pure polycaprolactone membrane,polycaprolactone/7.5%nanodiamond-phospholipid membrane,and polycaprolactone/7.5%polycaprolactone/nanodiamond membrane,respectively,to detect cell proliferation,adhesion,and osteogenic differentiation ability.RESULTS AND CONCLUSION:(1)The successful preparation of the polycaprolactone/nanodiamond-phospholipid composite material was confirmed by scanning electron microscopy and surface element composition.Compared with pure polycaprolactone membrane,the tensile strength of the polycaprolactone/7.5%nanodiamond-phospholipids membrane increased by 86.06%,the elastic modulus increased by 54.76%,and the water contact angle decreased to 70.0°,showing good physical and chemical properties.(2)The CCK-8 assay results showed that compared with pure polycaprolactone membrane and polycaprolactone/7.5%nanodiamond membrane,polycaprolactone/7.5%nanodiamond-phospholipid membrane could promote the proliferation of MC3T3-E1 cells.Phalloidine staining exhibited that compared with the polycaprolactone/7.5%nanodiamond membrane,the MC3T3-E1 cells on pure polycaprolactone and polycaprolactone/7.5%nanodiamond-phospholipid membrane were mostly rhomboid or spindle-shaped fibers,and the cells were more closely connected.Alkaline phosphatase staining showed that MC3T3-E1 cells on polycaprolactone/7.5%nanodiamond-phospholipid membrane exhibited stronger osteogenic differentiation ability compared with pure polycaprolactone membrane and polycaprolactone/7.5%nanodiamond membrane.(3)The results indicate that the polycaprolactone/nanodiamond-phospholipid composite material has good mechanical properties,hydrophilicity,biocompatibility,and the ability to promote osteogenic differentiation in vitro.

BACKGROUND:Polycaprolactone has been widely used in bone tissue engineering due to its excellent processing and degradation performance,but its poor hydrophilicity and mechanical strength cannot provide a good growth environment for osteoblasts.OBJECTIVE:To prepare polycaprolactone/nanodiamond-phospholipid composite materials,evaluate its biocompatibility and in vitro ability to promote bone differentiation.METHODS:Nanodiamond was modified using phospholipids,and polycaprolactone was used as the raw material.Polycaprolactone/nanodiamond-phospholipid composite materials with different mass ratios(0%,2.5%,7.5%,and 10%)were prepared by solution casting method.Polycaprolactone/nanodiamonds were used for comparison.The surface morphology,elemental composition,mechanical properties,and water contact angle of each group of materials were observed to select composite materials with better physical and chemical properties.MC3T3-E1 cells were inoculated onto pure polycaprolactone membrane,polycaprolactone/7.5%nanodiamond-phospholipid membrane,and polycaprolactone/7.5%polycaprolactone/nanodiamond membrane,respectively,to detect cell proliferation,adhesion,and osteogenic differentiation ability.RESULTS AND CONCLUSION:(1)The successful preparation of the polycaprolactone/nanodiamond-phospholipid composite material was confirmed by scanning electron microscopy and surface element composition.Compared with pure polycaprolactone membrane,the tensile strength of the polycaprolactone/7.5%nanodiamond-phospholipids membrane increased by 86.06%,the elastic modulus increased by 54.76%,and the water contact angle decreased to 70.0°,showing good physical and chemical properties.(2)The CCK-8 assay results showed that compared with pure polycaprolactone membrane and polycaprolactone/7.5%nanodiamond membrane,polycaprolactone/7.5%nanodiamond-phospholipid membrane could promote the proliferation of MC3T3-E1 cells.Phalloidine staining exhibited that compared with the polycaprolactone/7.5%nanodiamond membrane,the MC3T3-E1 cells on pure polycaprolactone and polycaprolactone/7.5%nanodiamond-phospholipid membrane were mostly rhomboid or spindle-shaped fibers,and the cells were more closely connected.Alkaline phosphatase staining showed that MC3T3-E1 cells on polycaprolactone/7.5%nanodiamond-phospholipid membrane exhibited stronger osteogenic differentiation ability compared with pure polycaprolactone membrane and polycaprolactone/7.5%nanodiamond membrane.(3)The results indicate that the polycaprolactone/nanodiamond-phospholipid composite material has good mechanical properties,hydrophilicity,biocompatibility,and the ability to promote osteogenic differentiation in vitro.

Objective To establish and verify nomogram prediction model with portal vein thrombosis in patients with cirrhosis,so as to evaluate its clinical application value.Methods The clinical data of 464 patients with cirrhosis with or without PVT admitted by our hospital from January 2015 to October 2022 were collected,univariate and multivariate Logistic regression analysis were used to screen the independent risk factors for PVT in patients with liver cirrhosis,and a nomogram prediction model was established,and the nomogram pre-diction model was verified for discrimination,calibration and clinical validity.Results Univariate and multivariate Logistic regression a-nalysis showed that the history of splenectomy,portal vein diameter,D-dimer and C-reactive protein-to-albumin ratio(CAR)were independent risk factors for cirrhosis with portal vein thrombosis(P＜0.05),the above independent risk factors were incorporated and a nomogram prediction model was successfully established.The nomogram was verified in both the modeling and validation populations:the area under the receiver operating characteristic(ROC)curve were 0.846(95％CI:0.803-0.888)and 0.815(95％CI:0.743-0.887),respectively,and the prediction model had good discrimination.The P-values in Hosmer-Lemeshow test were 0.783 and 0.453,respectively,and the model fitted well.The calibration chart showed that the nomogram model showed good calibration for the pre-diction probability of liver cirrhosis with PVT.Decision curve analysis(DCA)showed that the nomogram had good application value.Conclusion By establishing and validating a simple and practical nomogram prediction model for the risk of PVT in patients with cirrho-sis,it can help clinicians identify and intervene high-risk patients early.

Objective To explore the risk factors for patients with massive hemoptysis in tuberculosis and to provide a strategy for clinical treatment for tuberculosis massive hemoptysis (TMH). Methods Chi-square test and multivariate logistics analysis were applied to analyze the medical data of 241 cases of TMH. Results Chi-squared test showed that eleven factors were found to be significantly correlated with TMH. Longer disease course (≥3 months), lung lesions range ≥ 3 lung fields, pulmonary tuberculosis cavity, a higher smoking index (≥400 cigarettes per year) and clinical treatment were risk factors for TMH. Patients aged 45 years or older accompanied with bronchiectasia, pulmonary fungal infection, diabetes or hepatopathy had higher probabilities of developing massive hemoptysis. Multivariate analysis showed lung lesions range over 3 lung fields (OR = 2.447,P = 0.015), pulmonary tuberculosis cavity (OR = 2.486, P = 0.004), bronchial asthma (OR =3.192,P = 0.002), pulmonary fungal infection (OR = 3.896, P = 0.005) and hepatopathy (OR = 3.101, P =0.006) were final risk factors for TMH. Conclusion Multiple factors contributed to patients with massive hemoptysis in tuberculosis. Lung lesions range over 3 lung fields, pulmonary tuberculous cavities, bronchial asthma, lung fungal infection and hepatopathy might be the independent risk factors for TMH.