This study aimed to evaluate the usefulness of anatomic M-mode (AMM) in 31 healthy cats. We compared cardiac dimensions and function using two-dimensional (2D) mode, AMM, and conventional M-mode (CMM). We used 2D as the standard and evaluated the agreement among 2D, AMM, and CMM measurements. In healthy cats, AMM, rather than CMM, measurements of IVSd/s, LVDd/s, and LVFWd were acceptable for reliability. Since the diagnosis of HCM is based on thickness of IVSd and LVFWd, AMM has potential to be more useful than CMM as a diagnostic tool for echocardiographic measurement in cats.