Objective:To explore the application value of patient data-based real-time quality control (PBRTQC) in enhancing the effectiveness of internal quality control (IQC) management.Methods:From the PBRTQC real-time quality control intelligent monitoring platform integrated with the laboratory information system (LIS), a total of 35,631 test results of red blood cell (RBC) count, white blood cell (WBC) count, and dehydroepiandrosterone sulfate (DHEA-S) were collected from patients of the Department of General Xi'an Area Medical Laboratory Center from August 1, 2023, to April 1, 2024. The platform was used in patient data distribution characteristics test, EWMA real-time quality control chart procedure establishment, performance validation, effect evaluation, best procedure selection, and real-time operation. The performance evaluation indexes of the best PBRTQC procedure establishment, the cut-off limit range, weighting coefficient, cumulative mean, standard deviation (SD), coefficient of variation ( CV) of the EWMA real-time quality control chart, and the cumulative mean, SD, and CV of its internal quality control data in the same period were counted, and at the same time compared with the quality target (1/3TEa). Coefficient of variation analyses were performed to compare the quality control status of PBRTQC and conventional internal quality control in the presence of warning or alarm prompts based on quality control process records, and alarm messages. Results:The evaluation indexes of the optimal procedures for RBC count, WBC count, and DHEA-S were the probability of error detection (Ped) between 93%-97% and greater than 90%, the false positive rate (FPR) between 0.0%-0.5%, the false negative rate (FNR) between 3.0%-7.0%, and the average number of the patient sample until error detection (ANPed) between 5-11, which is in line with the optimal quality control efficacy quality requirements for the PBRTQC procedure. The patient outcome cut-off concentrations for the optimal procedure EWMA quality control charts ranged from RBC count (3.92-5.16)×10 12/L, WBC count (4.28-7.50)×10 9/L, and DHEA-S (830-2 160) μg/L; (2 160-4 210) μg/L. The weighting coefficients were 0.05, 0.03, and 0.03, respectively. The real-world application of the EWMA real-time quality control charts showed stable and excellent analytical performance of the measurement system, such as out-of-control alarm: RBC count, 1 true alarm, Ped of 95.85%, and FPR of 0%. The cumulative CV of EWMA was less than the quality target; the cumulative CV of DHEA-S was 7.66% and 9.47%, respectively, and the cumulative CV of low level was greater than the quality target (8.33%), and the cumulative CV of high and low levels were 4.12% and 6.25%. Conclusion:The PBRTQC EWMA method can monitor the patient data - in real-time and continuous way. It can also dynamically identify and provide early indication of small changes in analytical performance during the analysis process, and can be used as a supplement to quality control products to improve the efficacy of laboratory quality management.

Objective:To investigate the clinical and chest computed tomography (CT) findings of children with drug-resistant pulmonary tuberculosis (DR-PTB) and drug-sensitive pulmonary tuberculosis (DS-PTB) in Sichuan region.Methods:Active pulmonary tuberculosis children with positive sputum Mycobacterium tuberculosis culture were collected and divided into the drug-resistant and drug-sensitive groups according to the results of the drug susceptibility test who admitted to the Public Health Clinical Center of Chengdu from January 2020 to October 2024. The general data such as age, ethnic, gender, initial or retreatment regimens, body mass index (BMI), clinical symptoms such as cough, sputum, fever, and chest CT features were compared between the two groups. Non-parametric rank sum test, chi-square test or Fisher′s exact probability method were used for comparison between groups. The CT risk factors for the development of DR-PTB were analyzed using binary logistic regression. Results:A total of 110 children were enrolled, including 44(40.00%) males and 66(60.00%) females, with the age of 13.00(12.00, 14.00) years (range from 5.00 to 14.00 years). There were 28 cases in drug-resistant group and 82 in drug-sensitive group. Comparison of the clinical data between the two groups showed that the percentages of retreated patients (25.00%(7/28)) and ethnic minorities (78.57%(22/28)) in the drug-resistant group were higher than those in the drug-sensitive group (1.22%(1/82) and 57.32%(47/82)), which were both statistically significant (Fisher′s exact probability method and χ2=4.03, respectively, both P<0.05). The age of the children in the drug-resistant group was 13.00 (12.00, 13.50) years, which was younger than the age of 13.00 (12.00, 14.00) years in the drug-sensitive group ( U=828.00, P=0.021). There were no statistically differences of gender, BMI, history of bacillus Calmette-Guérin (BCG) vaccination, history of exposure to tuberculosis patients, positive immunological diagnostic tests, and the incidences of cough, sputum, fever, wheezing, shortness of breath and chest pain between the two groups (all P>0.05). Chest CT signs showed that lung lesions involving both right and left lobes accounted for 53.57%(15/28) in the drug-resistant group, and the incidence of solid pulmonary parenchyma (including wall-less cavities) was 92.86%(26/28), which were both higher than those in the drug-sensitive group (15.85%(13/82) and 70.73%(58/82)), and the differences were statistically significant ( χ2=6.95 and 5.66, respectively, both P<0.05). The incidence of small nodules/tree-in-bud pattern in the drug-sensitive group was higher than that in the drug-resistant group (98.78%(81/82) vs 82.14%(23/28)), and the difference was statistically significant (Fisher′s exact probability method, P=0.004). Binary logistic regression showed that bilateral lung involvement and solid pulmonary parenchyma (including wall-less cavities) were positively correlated with DR-PTB in children (odds ratio ( OR)=4.487, 95% confidence internal ( CI) 1.561 to 12.897, P=0.005 and OR=9.670, 95% CI 1.626 to 57.529, P=0.013, respectively). The occurence of small nodules/tree-in-bud was negatively correlated with DR-PTB in children ( OR=0.058, 95% CI 0.006 to 0.584, P=0.016). Conclusions:Among pulmonary tuberculosis children in Sichuan region, ethnic minorities and retreatment patients with DR-PTB are more common than thoses with DS-PTB. Chest CT findings of DR-PTB mainly show multiple solid pulmonary parenchyma with wall-less cavities, and the lesions often involve right and left lung lobes, whereas DS-PTB CT findings mainly show small nodules/tree-in-bud predominantly.

Objective:To investigate the clinical and chest computed tomography (CT) findings of children with drug-resistant pulmonary tuberculosis (DR-PTB) and drug-sensitive pulmonary tuberculosis (DS-PTB) in Sichuan region.Methods:Active pulmonary tuberculosis children with positive sputum Mycobacterium tuberculosis culture were collected and divided into the drug-resistant and drug-sensitive groups according to the results of the drug susceptibility test who admitted to the Public Health Clinical Center of Chengdu from January 2020 to October 2024. The general data such as age, ethnic, gender, initial or retreatment regimens, body mass index (BMI), clinical symptoms such as cough, sputum, fever, and chest CT features were compared between the two groups. Non-parametric rank sum test, chi-square test or Fisher′s exact probability method were used for comparison between groups. The CT risk factors for the development of DR-PTB were analyzed using binary logistic regression. Results:A total of 110 children were enrolled, including 44(40.00%) males and 66(60.00%) females, with the age of 13.00(12.00, 14.00) years (range from 5.00 to 14.00 years). There were 28 cases in drug-resistant group and 82 in drug-sensitive group. Comparison of the clinical data between the two groups showed that the percentages of retreated patients (25.00%(7/28)) and ethnic minorities (78.57%(22/28)) in the drug-resistant group were higher than those in the drug-sensitive group (1.22%(1/82) and 57.32%(47/82)), which were both statistically significant (Fisher′s exact probability method and χ2=4.03, respectively, both P<0.05). The age of the children in the drug-resistant group was 13.00 (12.00, 13.50) years, which was younger than the age of 13.00 (12.00, 14.00) years in the drug-sensitive group ( U=828.00, P=0.021). There were no statistically differences of gender, BMI, history of bacillus Calmette-Guérin (BCG) vaccination, history of exposure to tuberculosis patients, positive immunological diagnostic tests, and the incidences of cough, sputum, fever, wheezing, shortness of breath and chest pain between the two groups (all P>0.05). Chest CT signs showed that lung lesions involving both right and left lobes accounted for 53.57%(15/28) in the drug-resistant group, and the incidence of solid pulmonary parenchyma (including wall-less cavities) was 92.86%(26/28), which were both higher than those in the drug-sensitive group (15.85%(13/82) and 70.73%(58/82)), and the differences were statistically significant ( χ2=6.95 and 5.66, respectively, both P<0.05). The incidence of small nodules/tree-in-bud pattern in the drug-sensitive group was higher than that in the drug-resistant group (98.78%(81/82) vs 82.14%(23/28)), and the difference was statistically significant (Fisher′s exact probability method, P=0.004). Binary logistic regression showed that bilateral lung involvement and solid pulmonary parenchyma (including wall-less cavities) were positively correlated with DR-PTB in children (odds ratio ( OR)=4.487, 95% confidence internal ( CI) 1.561 to 12.897, P=0.005 and OR=9.670, 95% CI 1.626 to 57.529, P=0.013, respectively). The occurence of small nodules/tree-in-bud was negatively correlated with DR-PTB in children ( OR=0.058, 95% CI 0.006 to 0.584, P=0.016). Conclusions:Among pulmonary tuberculosis children in Sichuan region, ethnic minorities and retreatment patients with DR-PTB are more common than thoses with DS-PTB. Chest CT findings of DR-PTB mainly show multiple solid pulmonary parenchyma with wall-less cavities, and the lesions often involve right and left lung lobes, whereas DS-PTB CT findings mainly show small nodules/tree-in-bud predominantly.

Objective:To analyze the clinical features and mutation of myeloid differentiation factor 88 (MYD88) L265P in patients with diffuse large B-cell lymphoma (DLBCL) of central nervous system (CNS).Methods:The clinicopathological materials of 45 cases of DLBCL of CNS were retrospectively collected in Xuanwu Hospital, Capital Medical University from September 2014 to February 2017. The clinicopathological data were retrospectively analyzed, combined with immunohistochemistry, EB virus in situ hybridization, imaging and medical history. The mutation of MYD88 L265P gene was detected by pyrosequencing and its clinical significance was analyzed. Results:The age of the patients ranged from 42 to 82 years [(57.6±8.8) years], including 24 males and 21 females. Totally 93.3% (42/45) of the patients had supratentorial tumours, which were single or multiple. The cerebral hemisphere (31/45, 68.9%) was the most common involved site, and 21 cases (21/45, 46.7%) had multiple lesions. Histologically, DLBCL in the CNS showed diffuse infiltration of tumor tissue, some of which grew around blood vessels in a "sleeve" arrangement. CD 20 and CD 79a were diffusely and strongly positive. Thirty-nine cases (39/45, 86.7%) were non-germinal center B cell (non-GCB) subtype and 6 cases (6/45, 13.3%) were germinal center B cell (GCB) subtype. MYD88 L265P mutation was found in 64.4% (29/45) patients. There was statistically significant difference between non-GCB type (71.8%, 28/39) and GCB type DLBCL (1/6, P=0.017). Compared with the operation/biopsy group without chemotherapy, operation+chemotherapy, biopsy+chemotherapy, operation/biopsy+chemotherapy+stem cell transplantation can improve the survival and prognosis ( HR=0.05, 95% CI 0.01-0.33 , P=0.002; HR=0.04, 95% CI 0.01-0.36 , P=0.004; HR=0.01, 95% CI 0.00-0.17 , P=0.001; respectively). Conclusions:DLBCL of the CNS is aggressive tumor with poor prognosis, the clinical manifestations are complex and diverse, and the diagnosis is challenging. MYD88 L265P is a common and specific gene mutation in primary CNS lymphoma(PCNSL), which is of great significance in the diagnosis and treatment of lymphoma. The MYD88 L265P mutation was more frequently detected in non-GCB than GCB subtype. Chemotherapy can improve the survival rate of PCNSL patients. If chemotherapy achieves complete remission and autologous hematopoietic stem cell transplantation is performed, there may be a chance of long-term survival.

OBJECTIVES: To explore the effect of resveratrol (Res) on Kawasaki disease (KD)-induced myocardial injury and to evaluate its effect on apoptosis and autophagy. METHODS: Forty-eight juvenile male Sprague Dawley rats were randomly divided into a control group, a Res group, a lactobacillus casei cell wall extract (LCWE)-induced Kawasaki disease group (KD group), and a LCWE-induced Kawasaki disease + Res treatment group (Res+KD group). The control group was intraperitoneally injected with saline. The Res group was intraperitoneally injected with resveratrol (100 mg/kg). The KD group was intraperitoneally injected with 0.5 mL LCWE (1 mg/mL). The Res+KD group was intraperitoneally injected with 0.5 mL LCWE (1 mg/mL) and resveratrol (100 mg/kg). After 4 weeks, the left ventricular ejection fraction (LVEF) and short axis shortening rate (LVFS) were detected by echocardiography. The apoptotic rate was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) staining. The levels of B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), microtubule-associated protein 1 light chain 3β (LC3B), Beclin-1, autophagy related 5 (Atg5) and sequestosome-1 (p62) were detected by Western blotting. The formation of autophagosome was observed under transmission electron microscope. RESULTS: There was no significant difference in the above-mentioned indexes between the control group and the Res group (all CONCLUSIONS Res can attenuate the KD-induced myocardial injury via inhibiting the apoptosis and autophagy.
Animals ; Apoptosis ; Autophagy ; Male ; Mucocutaneous Lymph Node Syndrome/drug therapy* ; Rats ; Rats, Sprague-Dawley ; Resveratrol/therapeutic use* ; Stroke Volume ; Ventricular Function, Left

Genomic alterations are commonly found in the signaling pathways of fibroblast growth factor receptors (FGFRs). Although there is no selective FGFR inhibitors in market, several promising inhibitors have been investigated in clinical trials, and showed encouraging efficacies in patients. By designing a hybrid between the FGFR-selectivity-enhancing motif dimethoxybenzene group and our previously identified novel scaffold, we discovered a new series of potent FGFR inhibitors, with the best one showing sub-nanomolar enzymatic activity. After several round of optimization and with the solved crystal structure, detailed structure-activity relationship was elaborated. Together with metabolic stability tests and pharmacokinetic profiling, a representative compound () was selected and tested in xenograft mouse model, and the result demonstrated that inhibitor was effective against tumors with FGFR genetic alterations, exhibiting potential for further development.

Objective To investigate an efficient rapid method for the isolation and cultivation of human axillary dermal papilla cells.Methods Skin specimens with hair follicles were obtained from the axillary area of patients who received bromhidrosis surgery in the Department of Dermatology of the First Affiliated Hospital to Army Medical University from October 2015 to May 2016.The axillary dermal papilla cells were isolated by two-step enzyme digestion method,one-step digestion method and micro-dissection method separately.Then,axillary dermal papilla cells were cultured and identified.Differences in the operative procedure,separation efficiency and adhesion efficiency of dermal papilla cells,cell emigration duration,total operation duration and actual operation duration were compared among the above 3 methods.Results Compared with the one-step digestion method and micro-dissection method,the two-step enzyme digestion method showed simpler operative procedure,more than 30％ separation rate and 96％ adhesion rate of dermal papilla cells after 1 week.Moreover,the cell emigration duration was shortened by 3-4 days by the two-step enzyme digestion method.The two-step enzyme digestion method also showed longer total operation duration,but shorter actual operation duration compared with the one-step digestion method and micro-dissection method,as well as lower contamination rate compared with the micro-dissection method.Cultured axillary dermal papilla cells grew in an aggregative pattern in the early stage,but grew in a nonaggregative pattern after 6 passages.Immunofluorescence assay showed positive staining for laminin and collagen Ⅳ in axillary dermal papilla cells.Conclusion The modified two-step enzyme digestion method is a kind of simple,efficient and rapid method for the isolation of human axillary dermal papilla cells,and axillary dermal papilla cells can be harvested through this method by using a few specimens.

Objective:To study the therapeutic effect of a novel double-target system,in which human umbilical cord-derived MSCs were used as vehicles to deliver fusion protein scFvCD20:sTRAIL to non-Hodgkin ’ s lymphoma. Methods: The traditional methods in molecular biology were used to construct lentivirus expression vectors pLenR. scFvCD20: sTRAIL and contrast vectors. Human umbilical cord-derived MSCs ( HUMSCs ) were labeled with the copGFP by transducing with pseudo viral particles which had been packaged in 293T cells with four plasmid-lentivirus packaging system. Fusion protein scFvCD20:sTRAIL were secreted from MSC. scFvCD20:sTRAIL after that HUMSCs were infected by pseudo viral particles. CCK8 assay was applied to detect the antigen-restricted cell death induced by scFvCD20:sTRAIL in CD20-positive BJAB and Raji cells as well as CD20-negtive Jurkat cells and human normal peripheral blood mononuclear cells (PBMCs). To evaluate the therapeutic effect of MSC. scFvCD20:sTRAIL in vivo,ge-netically modified HUMSCs were intravenously injected into tumor-bearing mice with BJAB cells. The volume of tumor was measured every three days, and the inhibition ratio of tumor was calculated according to tumor volume. Results: Lentivirus expression vectors pLenR. scFvCD20:sTRAIL, pLenR. ISZ:sTRAIL, pLenR. scFvCD20 and pLenR. CopGFP were successfully constructed and these constructs could be expressed stably in HUMSCs by lentivirus transduction. scFvCD20:sTRAIL fusion protein produced a potent inhibition of cell proliferation in CD20-positive BJAB cells,moderate inhibition of the growth of Raji cells,and weak inhibition in CD20-negtive Jurkat cells when compared with ISZ-sTRAIL treatment,and it had no effect on normal human peripheral blood mononuclear cells (PBMCs). The MSC. scFvCD20:sTRAIL treatment significantly inhibited the tumor growth when compared with those treated with MSC. ISZ-sTRAIL. Conclusion: A double-target therapeutic system is well established, in which HUMSCs migrated to tumor site, secreted a novel fusion protein scFvCD20:sTRAIL,and thus locally concentrated scFvCD20:sTRAIL extended antigen-restricted anti-tumor activity. The engineered HUMSCs secreting scFvCD20:sTRAIL showed potent effect on inhibiting tumor growth in BJAB lymphoma malignancy,which may play an essential role in the clinical research .

Aim To explore the anti-proliferation effects of curcumin trinicotinate ( CurTn ) on vascular smooth muscle cell ( VSMC ) and its mechanism. Methods The cells were cultured in DMEM supple-mented with 10% fetal bovine serum. MTT assay was used to examine cell proliferation. FCM was used to observe cell cycle. The expressions of PCNA, Cy-clinD1 and p-ERK1/2 were analyzed using Western blot. Results CurTn could inhibit the proliferation of VSMC and showed a certain amount-time relationship. What’ s more, CurTn could increase the G1 phase pro-portion of cell, decrease the S phase proportion and the expression level of PCNA protein. It was also found that CurTn significantly inhibit the protein expression of p-ERK1/2 and Cyclin D1 . Conclusion CurTn may inhibit the proliferation of VSMC via downregulating the expression of CyclinD1 and p-ERK1/2 .

Objective To explore the related influencing factors and nursing countermeasures of venous thrombus in tumour patients with PICC .Methods We retrospectively analyzed the clinical data of 423 cancer patients with PICC of 2014 along with calculating the incidence of venous thrombus during intubation and related risk factors.Results There were 16 patients with venous thrombus (16/423) during intubation accounting for 3.87%.Besides, the risk factors of venous thrombus included the age of patients , tumour stage, position of catheter tip, previous history of thrombus , lack of activity, obesity, low hemoglobin, short time in activated partial thromboplastin ( APTT ) .Conclusions Pay more attention to the thrombosis complications after intubation in tumour patients .Besides , a variety of measures aiming at the risk factors should be taken actively to prevent and deal with the thrombosis .