Early correction of childhood malocclusion is timely managing morphological, structural, and functional abnormalities at different dentomaxillofacial developmental stages. The selection of appropriate imaging examination and comprehensive radiological diagnosis and analysis play an important role in early correction of childhood malocclusion. This expert consensus is a collaborative effort by multidisciplinary experts in dentistry across the nation based on the current clinical evidence, aiming to provide general guidance on appropriate imaging examination selection, comprehensive and accurate imaging assessment for early orthodontic treatment patients.
Humans ; Malocclusion/diagnostic imaging* ; Child ; Consensus

Objective To establish a portable gas chromatography-mass spectrometry (GC-MS) method for determining carbon disulfide in workplace air. Methods Samples were collected using the built-in Tenax GR adsorption tube in the portable GC-MS, followed by thermal desorption. The analytes were separated on a DB-1 chromatographic column and detected by a 3D ion trap mass spectrometer, with 1,3,5-tris(trifluoromethyl)benzene used as the internal standard. Qualitative analysis was based on retention time and characteristic ions, and quantitative analysis was performed using the internal standard method. Results The method showed a linear range of 0.034-0.340 mg/m³ with a correlation coefficient of 0.999 4 using the adsorption tube enrichment mode. The detection limit was 0.007 mg/m³, and the lower limit of quantification was 0.022 mg/m³. The average recovery ranged from 97.5% to 104.0%. The within-run and between-run relative standard deviation was 2.7%-10.4% and 8.8%-14.8%, respectively. Conclusion A rapid, green, highly sensitive, and interference-resistant on-site detection method was established. As a supplement to existing national standard methods, this method is suitable for real-time monitoring of carbon disulfide in workplace air and for occupational exposure risk assessment.

OBJECTIVE To compare the efficacy and safety of lacosamide （LCM） and carbamazepine （CAR） as monotherapy in the treatment of adult patients with newly diagnosed epilepsy. METHODS By methods of retrospective analysis， 84 adult patients with newly diagnosed epilepsy， were admitted to the Department of Neurology， Huaihe Hospital of Henan University during Sept. 2020-Jun. 2022， were divided into the control group （40 cases， receiving CAR treatment） and the observation group （44 cases， receiving LCM treatment） according to different medication regimens. Total response rate， epilepsy seizure frequency， blood lipid levels， and the occurrence of adverse events （AEs） of patients were compared between the 2 groups. RESULTS In the first month after treatment， there was no statistically significant difference in the total response rate between the observation group （63.64%） and the control group （55.00%， P＞0.05）； the frequency of epilepsy seizure in both groups was significantly reduced compared to before treatment （P＜0.05）， but there was no statistically significant difference between 2 groups （P＞0.05）. In the third month after treatment， the total response rate of the observation group （90.91%） was significantly higher than control group （67.50%， P＜0.05）； the frequencies of epilepsy seizure in both groups were significantly reduced compared to before treatment， and the observation group was significantly lower than the control group （P＜0.05）. In the third month after treatment， the levels of total cholesterol （TC）， triglyceride （TG） and low-density lipoprotein cholestrol （LDL-C） in the control group and the level of LDL-C in the observation group were significantly higher than before treatment， and the levels of TC， TG and LDL-C in the observation group were significantly lower than those in the control group （P＜0.05）. There was no statistically significant difference in the incidence of AEs between the observation group （15.91%） and the control group （17.50%， P＞0.05）. CONCLUSIONS Both LCM and CAR have certain effects in the treatment of newly diagnosed epilepsy in adults， which can reduce the frequency of epilepsy seizure in patients and have comparable safety. Meanwhile， LCM has better long-term efficacy than CAR in treating newly diagnosed epilepsy in adults， and its impact on the patient’s blood lipid is smaller than CAR.

Objective:To investigate the effects of oleuropein (OLE) on the migration, invasion, and chemotherapy sensitivity of cervical cancer cells by regulating the phosphatidylinositol 3 kinase-protein kinase B (PI3K-AKT) signaling pathway.Methods:SiHa cells were treated with 0-80 μ mol/L OLEs to detect cell survival rate and screen for the optimal drug concentration. SiHa cells were randomly separated into SiHa group, OLE low concentration (OLE-L) group, OLE medium concentration (OLE-M) group, OLE high concentration (OLE-H) group, and OLE-H+PI3K activator 740Y-P (OLE-H+740Y-P) group. CCK-8 method was applied to detect the proliferation activity of cells in each group. Transwell experiment was applied to detect the migration and invasion abilities of cells in each group. Flow cytometry was applied to detect the apoptosis rate of cells in each group. Western blot was used to detect the expression levels of PI3K-AKT signaling pathway related proteins, epithelial mesenchymal transition (EMT), and resistance related proteins such as epithelial cadherin, vimentin, N-cadherin, and P-glycoprotein (P-gp) of cells in each group.Results:OLEs with concentrations of 5, 10, and 20 μmol/L were selected for subsequent experiments. Compared with the SiHa group, the survival rate, numbers of clone formation, migration and invasion of cells, and the expression levels of p-PI3K/PI3K, p-AKT/AKT, Vimentin, N-cadherin and P-gp in the OLE-L, OLE-M, and OLE-H groups gradually decreased ( P<0.05), while the apoptosis rate and E-cadherin expression level increased ( P<0.05). Compared to that before cisplatin treatment, the apoptosis rate of SiHa cells increased after cisplatin treatment ( P<0.05). The addition of PI3K activator 740Y-P on the basis of high concentration OLE reversed the trend of changes in the above indicators ( P<0.05) . Conclusions:OLE can inhibit the migration and invasion of cervical cancer cells, and improve their chemotherapy sensitivity. Its mechanism of action may be related to the inhibition of the PI3K-AKT signaling pathway.

Objective:To investigate the effects of oleuropein (OLE) on the migration, invasion, and chemotherapy sensitivity of cervical cancer cells by regulating the phosphatidylinositol 3 kinase-protein kinase B (PI3K-AKT) signaling pathway.Methods:SiHa cells were treated with 0-80 μ mol/L OLEs to detect cell survival rate and screen for the optimal drug concentration. SiHa cells were randomly separated into SiHa group, OLE low concentration (OLE-L) group, OLE medium concentration (OLE-M) group, OLE high concentration (OLE-H) group, and OLE-H+PI3K activator 740Y-P (OLE-H+740Y-P) group. CCK-8 method was applied to detect the proliferation activity of cells in each group. Transwell experiment was applied to detect the migration and invasion abilities of cells in each group. Flow cytometry was applied to detect the apoptosis rate of cells in each group. Western blot was used to detect the expression levels of PI3K-AKT signaling pathway related proteins, epithelial mesenchymal transition (EMT), and resistance related proteins such as epithelial cadherin, vimentin, N-cadherin, and P-glycoprotein (P-gp) of cells in each group.Results:OLEs with concentrations of 5, 10, and 20 μmol/L were selected for subsequent experiments. Compared with the SiHa group, the survival rate, numbers of clone formation, migration and invasion of cells, and the expression levels of p-PI3K/PI3K, p-AKT/AKT, Vimentin, N-cadherin and P-gp in the OLE-L, OLE-M, and OLE-H groups gradually decreased ( P<0.05), while the apoptosis rate and E-cadherin expression level increased ( P<0.05). Compared to that before cisplatin treatment, the apoptosis rate of SiHa cells increased after cisplatin treatment ( P<0.05). The addition of PI3K activator 740Y-P on the basis of high concentration OLE reversed the trend of changes in the above indicators ( P<0.05) . Conclusions:OLE can inhibit the migration and invasion of cervical cancer cells, and improve their chemotherapy sensitivity. Its mechanism of action may be related to the inhibition of the PI3K-AKT signaling pathway.

Prostate cancer is one of the most common malignant tumors in men,with rapidly increasing incidence and mortality rates. Cur-rently,the advisability of performing prostate biopsy for men with PI-RADS scores of 1-3 is controversial. Aggressive biopsy may lead to over diagnosis,increasing patient suffering,whereas active surveillance may increase late diagnosis and disease progression. Accurately identify-ing cases that merit prostate biopsy is an important challenge in clinical practice. Recently,numerous studies have explored whether indi-viduals with PI-RADS scores of 1-3 should undergo prostate biopsy,but this recent research has not yet been systematically summarized and discussed. In this article,we summarize predictive factors indicating prostate biopsy in the PI-RADS 1-3 population and discuss their predict-ive value to provide new guidance for clinical practice and further research.

Prostate cancer is one of the most common malignant tumors in men,with rapidly increasing incidence and mortality rates. Cur-rently,the advisability of performing prostate biopsy for men with PI-RADS scores of 1-3 is controversial. Aggressive biopsy may lead to over diagnosis,increasing patient suffering,whereas active surveillance may increase late diagnosis and disease progression. Accurately identify-ing cases that merit prostate biopsy is an important challenge in clinical practice. Recently,numerous studies have explored whether indi-viduals with PI-RADS scores of 1-3 should undergo prostate biopsy,but this recent research has not yet been systematically summarized and discussed. In this article,we summarize predictive factors indicating prostate biopsy in the PI-RADS 1-3 population and discuss their predict-ive value to provide new guidance for clinical practice and further research.

Iron (Fe) is an important trace element involved in many important plant physiological and metabolic processes such as photosynthesis, respiration and nitrogen metabolism. Plants maintain iron homeostasis through absorption, transporting, storage and redistribution of iron. Iron metabolism is strictly regulated in plants. Iron regulatory transcription factors and iron transporters constitute the regulatory network of plant iron absorption and transport in plants. Ferritin and iron transporter jointly regulate the response to excess iron in plants. In recent years, important progress has been made in understanding how abscisic acid (ABA) regulates iron metabolism in plants. ABA may be used as a signal to regulate the absorption, transportation and reuse of Fe, or to relieve the symptoms of iron stress by regulating the oxidative stress responses in plants. In order to gain deeper insights into the crosstalk of ABA and iron metabolism in plants, this review summarized the mechanisms of iron absorption and transport and metabolic regulatory network in plants, as well as the mechanisms of ABA in regulating iron metabolism. The relationship between ABA and FER-like iron deficiency-induced transcription factor (FIT), iron-regulated transporter 1 (IRT1), and oxidative stress of iron deficiency were highlighted, and future research directions were prospected.
Abscisic Acid/metabolism* ; Gene Expression Regulation, Plant ; Homeostasis ; Iron/metabolism* ; Plants/metabolism* ; Transcription Factors/metabolism*

Objective:To investigate the risk factors of relapse after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in patients with t (8;21) acute myeloid leukemia (AML) .Methods:The clinical features of patients with t (8;21) AML who received allo-HSCT between January 2008 and October 2020 in the Hospital of Blood Disease and the Chinese Academy of Medical Sciences were retrospectively analyzed. Univariate and multivariate analyses were performed on the factors that might influence relapse.Results:A total of 73 patients were enrolled. The analysis revealed that, out of the 73 cases, 10 had relapses, with a 3-year cumulative incidence of relapse (CIR) of 15.7% (95% CI 7.3%-26.8%) . The median time of relapse was 9.2 (2.0-47.6) months. Furthermore, 19 cases died, with a 3-year overall survival (OS) of 68.9% (95% CI 56.4%-81.4%) . Compared with the RUNX1-RUNX1T1 at first diagnosis, a ≥ 3-log reduction within 3 months and/or 4-log reduction within 3-12 months can significantly decrease 3-year CIR after HSCT (13.3% vs 57.1%; 5.1% vs 25.0%, both P<0.001) . Cox multivariate analysis showed that high levels of RUNX1-RUNX1T1 (≥1.58%) on the day of transplantation (day 0) [ P=0.006; HR=28.849 (95% CI 2.68-310.524) ] and the flow cytometric analysis of blasts ratio in bone marrow ≥60% at first diagnosis [ P=0.015; HR=6.64 (95% CI 1.448-30.457) ] were independent risk factors for relapse. Furthermore, no significant difference in the effect of c-Kit and Flt3 gene mutations on relapse after transplantation was observed ( P=0.877 and P=0.773, resp) . The flow cytometric analysis of blasts ratio in bone marrow ≥60% at first diagnosis [ P<0.001; HR=8.925 (95% CI 2.702-29.476) ] and the number of courses to achieve complete remission ≥ 2[ P=0.013; HR=4.495 (95% CI 1.379-14.649) ] were independent risk factors for OS. Conclusion:Both high levels of RUNX1-RUNX1T1 (≥1.58%) on the day of transplantation (day 0) and the ratio of flow cytometric analysis of blasts in bone marrow at first diagnosis increase the chance of t (8;21) AML relapse after allo-HSCT. Detection of the transcription levels of RUNX1-RUNX1T1 after allo-HSCT at different times could help predict the hazard of relapse.

Objective:To investigate the effect of low expression of human epidermal growth factor-like domain protein 7 (EGFL7) gene in cervical cancer cell Hela on its migration and invasion ability.Methods:Cells in the experimental group used small interfering RNA (siRNA) to target the human EGFL7 gene to reduce the expression of EGFL7 in human cervical cancer cells Hela, and cells in the control group were transfected with Mock-siRNA. Real-time quantitative PCR was used to detect the EGFL7 mRNA content of cancer cells in each group; Western blot was used to detect EGFL7 protein expression of cancer cells in each group; The cell scratch healing experiment and Transwell experiment were used to detect the migration and invasion ability of Hela cells in each group.Results:siRNA reduced the protein expression of EGFL7 in human cervical cancer cell Hela. The wound closure percentage of Hela cells in the control group was 74.1%±6.8%. After the expression of EGFL7 was reduced, the percentage of cervical cancer cells was 42%±4.9%, and the wound closure ability was significantly reduced ( P<0.05) . The results of Transwell cell transfer showed that the number of cells successfully transferred by Hela cells in the control group was 179.24±20.01, while the number of cells successfully transferred by Hela cells with low EGFL7 expression was 79.22±13.16. The transfer ability of cells transfected with EGFL7-siRNA was significantly reduced ( P<0.05) . The results of invasion experiments showed that the number of successfully transferred cells in the control group was 79.35±8.04, the number of cells successfully transferred in the EGFL7-siRNA group was 26.98±6.24, and the invasion ability of Hela cells with low expression of EGFL7 decreased ( P< 0.05) . The expression of E-cad in Hela cells with low expression of EGFL7 was up-regulated, and the expression of MMP2/9 protein was down-regulated (all P<0.05) . Conclusion:The low expression of EGFL7 can reduce the migration and invasion ability of cervical cancer cell Hela through the EMT pathway.