Objective To discuss the application value of XperCT combined with needle-guided Glubran-2 glue for small pulmonary nodule localization in thoracoscopic pulmonary nodule resection.Methods The clinical data of 67 patients,who received XperCT combined with needle-guided Glubran-2 glue for small pulmonary nodule localization before thoracoscopic resection of a single small pulmonary nodule at the Jining Municipal First People's Hospital of China between June 2018 and February 2023,were retrospectively analyzed.The size of the pulmonary nodule,the maximum vertical distance from the visceral pleura to the lesion,the technical success rate of localization,the number of puncturing times,the complications,the time spent for operation,and the postoperative pathological diagnosis were recorded.Results The average size of the small pulmonary nodules in the 67 patients was 8.7 mm,and the average vertical distance from the visceral pleura to the lesion was 19.4 mm.Successful preoperative localization of nodule was accomplished in all patients.The average number of puncturing times was 1.1,and no serious complications occurred.The average time spent for operation was 12.7 min.Definite pathological results were obtained in all 67 patients.Conclusion XperCT combined with needle-guided Glubran-2 glue for small pulmonary nodule localization carries advantage of accurate localization with fewer complications.Therefore,this technique is a highly-efficient and quickly-accomplished positioning method,and it is highly valuable in clinical practice.(J Intervent Radiol,2024,33:623-626)

Objective To observe the effect of transcatheter arterial embolization(TAE)with Glubran-2 glue for treating hemorrhage after percutaneous transhepatic cholangial drainage(PTCD).Methods Data of 17 patients with hemorrhage after PTCD who underwent TAE with Glubran-2 glue were retrospectively analyzed.The technical success rate,clinical success rate and complications were observed.The red blood cell(RBC)and hemoglobin(Hb)on the day of TAE and the next day of TAE were compared,also the glutamic-pyruvic transaminase(GPT)level before TAE,on the next day of TAE,on the second and the fourth day after TAE,respectively.Results The offending vessel of bleeding was successfully embolized in all 17 cases,both technical success rate of TAE and clinical success rate of hemostasis were 100%.There was no serious complication such as liver abscess,septicemia nor pulmonary embolism.No significant difference of RBC nor Hb was found between the day of TAE and the next day of TAE(both P＞0.05).GPT before TAE was lower than the next day of TAE and the second day after TAE(P＜0.05),while no significant difference of GPT was found before TAE and 4 days after TAE(P＞0.05).Conclusion TAE with Glubran-2 glue for treating hemorrhage after PTCD was safe and effective.

Deep sternal wound infection (DSWI) is complicated and infection might affect multiple layers. It is difficult to identify and locate the degree and range of infection accurately before the operation. Thus, complete debridement tends to be challenging because of potential above-mentioned issues. Then it would lead to recurrence and further deterioration of the disease postoperatively. Timely and efficient diagnostic methods could promote early diagnosis of DSWI, and locate the focus of infection accurately so as to deal with it in time. This paper compares and summarizes the existing diagnostic methods for DSWI at home and abroad, such as microbiological examination, imaging examination, clinical symptoms and signs, in order to improve the efficiency of diagnosis and treatment of DSWI.

Deep sternal wound infection (DSWI) is complicated and infection might affect multiple layers. It is difficult to identify and locate the degree and range of infection accurately before the operation. Thus, complete debridement tends to be challenging because of potential above-mentioned issues. Then it would lead to recurrence and further deterioration of the disease postoperatively. Timely and efficient diagnostic methods could promote early diagnosis of DSWI, and locate the focus of infection accurately so as to deal with it in time. This paper compares and summarizes the existing diagnostic methods for DSWI at home and abroad, such as microbiological examination, imaging examination, clinical symptoms and signs, in order to improve the efficiency of diagnosis and treatment of DSWI.

Deep sternal wound infection (DSWI) is complicated and infection might affect multiple layers. It is difficult to identify and locate the degree and range of infection accurately before the operation. Thus, complete debridement tends to be challenging because of potential above-mentioned issues. Then it would lead to recurrence and further deterioration of the disease postoperatively. Timely and efficient diagnostic methods could promote early diagnosis of DSWI, and locate the focus of infection accurately so as to deal with it in time. This paper compares and summarizes the existing diagnostic methods for DSWI at home and abroad, such as microbiological examination, imaging examination, clinical symptoms and signs, in order to improve the efficiency of diagnosis and treatment of DSWI.

Deep sternal wound infection (DSWI) is complicated and infection might affect multiple layers. It is difficult to identify and locate the degree and range of infection accurately before the operation. Thus, complete debridement tends to be challenging because of potential above-mentioned issues. Then it would lead to recurrence and further deterioration of the disease postoperatively. Timely and efficient diagnostic methods could promote early diagnosis of DSWI, and locate the focus of infection accurately so as to deal with it in time. This paper compares and summarizes the existing diagnostic methods for DSWI at home and abroad, such as microbiological examination, imaging examination, clinical symptoms and signs, in order to improve the efficiency of diagnosis and treatment of DSWI.

Wound healing is a complex pathophysiological process. Vitamin can promote wound healing from different aspects and in multiple stages. This paper reviews the role of vitamin A, B, C, D, E in promoting wound healing and the related mechanism.

Wound healing is a complex pathophysiological process. Vitamin can promote wound healing from different aspects and in multiple stages. This paper reviews the role of vitamin A, B, C, D, E in promoting wound healing and the related mechanism.

Objective To evaluate the effect of plastic surgery for scar contracture after hand burn.Methods Clinical treatment data of 56 patients with scar contracture after hand burn was collected from December 2011 to December 2016.Different surgery methods were adopted to treat the scar contracture after hand burn according to different degree,different sites and different range.Results All cases were operated successfully,and the appearance and function of the hands were almost recovered during the 6 months' follow-up,without contracture again.Conclusion Appropriate surgical programs should be used in scar contracture with different parts and degrees,and reasonable postoperative rehabilitation can improve the function and appearance of hands better,which can avoid secondum contracture.

Objective: To explore an optimal method for granulocyte cell production from umbilical cord blood mononuclear cells. Methods: Erythrocytes were precipitated by hydroxyethyl starch. Mononuclear cells were isolated through Ficoll density gradient centrifugation. Different media, additives and cultivation model were chosen for granulocyte induction. Cell morphology was observed by microscopy, and cell phenotype was detected by flow cytometry. The CD18 expression of granulocytes was tested by immunofluorescence assay, and phagocytosis test was executed as well. Results: Compared to fetal bovine serum (FBS) treatment group, cell viability, counts and differentiation rate of granulocytes induced by X-VIVO^TM 15 combined with TPO, SCF, G-CSF but without FBS were superior. And X-VIVO^TM15 medium was better than SCGM medium at effectiveness and cost. Using two-stage mode of hematopoietic stem cell expansion followed by granulocyte induction with X-VIVO^TM15 combining TPO, SCF and G-CSF, cell proliferation was nearly 132 times at day 21. Flow cytometry showed that the differentiation was lagged in 2-stage mode than in direct induction mode, CD15 expression was (69.60± 1.06) % vs (97.73±0.39) %; Wright-Giemsa staining demonstrated mature granulocytes; immunofluorescence showed the expression of lysosomal proteins CD18. A strong phagocytic function of mature granulocytes was demonstrated by phagotrophic efficiency of (51.43±0.05) %. And granulocyte had chemotaxis ability under the role of chemotactic factor IL-8. Conclusion Optimized culture media and cultivation mode are achieved for functional granulocytes induction in vitro.