Objective:To analyze the application effect of hyperbaric oxygen chamber management system based on five-dimensional quality management model included safety,reliability,effectiveness,availability and economy of equipment in management for the equipment of hyperbaric oxygen chamber management. Methods:The hyperbaric oxygen chamber management system was built on the basis of five-dimensional quality management model,and an assessment indicator system was constructed to perform quality control evaluation with five-dimensional maturities included preparation mode,thinking mode,communication mode,action mode and review mode. Then,a whole process management was conducted for the equipment of hyperbaric oxygen chamber. A hyperbaric oxygen chamber that was used during 2022 and 2023 in The 940th Hospital of People's Liberation Army Joint Service Support Force was selected,and the conventional management was used to manage the equipment during January and October 2022,and the hyperbaric oxygen chamber management system based on five-dimensional quality management model (model management) was used to manage the equipment during January and October 2023. A total of 400 operation logs of hyperbaric oxygen chamber equipment were selected,which included 200 logs of each mode,and the timeliness score,the satisfaction score of relevant personnel and the effect of equipment management between the two models were compared. Results:The timeliness scores of matching ratio of equipment,reasonable configuration of equipment,standard arrangement of equipment and equipment maintenance of model management were respectively (9.26±1.21) points,(9.12±0.98) points,(9.26±1.36) points and (9.36±2.03) points for hyperbaric oxygen chamber,which were higher than those of conventional management,and the differences were statistically significant (t=16.107,15.481,18.671,6.971,P<0.05). The satisfaction scores of operators,operation and maintenance personnel and engineers of equipment of model management were respectively (93.62±4.63) points,(92.69±4.33) points and (95.33±4.36) points,which were higher than those of conventional management method,and the differences of them between two methods were statistically significant (t=17.711,10.082,10.197,P<0.05). In the 200 logs of adopting model management,the ratio of complete files and good operation of equipment were respectively 95.0％ and 94.0％,which were higher than those of conventional management method. While the ratio of operation without standard and abnormal operation of equipment were respectively 1.0％ and 1.5％,which were lower than those of conventional management methods,and the differences of them were statistically significant (x2=14.709,12.754,11.402,11.308,P<0.05). Conclusion:The application of the hyperbaric oxygen chamber management system based on the five-dimensional quality management model in the management of hyperbaric oxygen chamber equipment can maximum improve the operating efficiency of hyperbaric oxygen chamber equipment,and effectively increase the quality and effect of equipment management,and enhance the operation satisfaction of relevant personnel.

Objective:To analyze the application effect of hyperbaric oxygen chamber management system based on five-dimensional quality management model included safety,reliability,effectiveness,availability and economy of equipment in management for the equipment of hyperbaric oxygen chamber management. Methods:The hyperbaric oxygen chamber management system was built on the basis of five-dimensional quality management model,and an assessment indicator system was constructed to perform quality control evaluation with five-dimensional maturities included preparation mode,thinking mode,communication mode,action mode and review mode. Then,a whole process management was conducted for the equipment of hyperbaric oxygen chamber. A hyperbaric oxygen chamber that was used during 2022 and 2023 in The 940th Hospital of People's Liberation Army Joint Service Support Force was selected,and the conventional management was used to manage the equipment during January and October 2022,and the hyperbaric oxygen chamber management system based on five-dimensional quality management model (model management) was used to manage the equipment during January and October 2023. A total of 400 operation logs of hyperbaric oxygen chamber equipment were selected,which included 200 logs of each mode,and the timeliness score,the satisfaction score of relevant personnel and the effect of equipment management between the two models were compared. Results:The timeliness scores of matching ratio of equipment,reasonable configuration of equipment,standard arrangement of equipment and equipment maintenance of model management were respectively (9.26±1.21) points,(9.12±0.98) points,(9.26±1.36) points and (9.36±2.03) points for hyperbaric oxygen chamber,which were higher than those of conventional management,and the differences were statistically significant (t=16.107,15.481,18.671,6.971,P<0.05). The satisfaction scores of operators,operation and maintenance personnel and engineers of equipment of model management were respectively (93.62±4.63) points,(92.69±4.33) points and (95.33±4.36) points,which were higher than those of conventional management method,and the differences of them between two methods were statistically significant (t=17.711,10.082,10.197,P<0.05). In the 200 logs of adopting model management,the ratio of complete files and good operation of equipment were respectively 95.0％ and 94.0％,which were higher than those of conventional management method. While the ratio of operation without standard and abnormal operation of equipment were respectively 1.0％ and 1.5％,which were lower than those of conventional management methods,and the differences of them were statistically significant (x2=14.709,12.754,11.402,11.308,P<0.05). Conclusion:The application of the hyperbaric oxygen chamber management system based on the five-dimensional quality management model in the management of hyperbaric oxygen chamber equipment can maximum improve the operating efficiency of hyperbaric oxygen chamber equipment,and effectively increase the quality and effect of equipment management,and enhance the operation satisfaction of relevant personnel.

The DNA characterization of Cannabis sativa L.has been one of the key directions of anti-drug research at home and abroad.Previous research mainly focused on the identification of cannabis-species and gender differentiation,and have constructed a number of corresponding composite amplification systems.With the rapid development of high-throughput sequencing technology,the whole genome of C.sativa and the sequences of key enzyme genes for its major physicochemical components have been sequenced successively,and intra-species differentiation studies of C.sativa based on specific molecular markers have gradually emerged.However,due to the high variability of cannabis subspecies-and variety-specific molecular markers,relevant foreign studies failed to provide ideal molecular marker support for the identification of intra-specific distinctions of Cannabis sativa in China.Based on this,this paper comprehensively analyzes the current situation and shortcomings of domestic and international research on intra-specific differentiation of C.sativa,and combines the previous research results of this group to elaborate on how to use high-throughput sequencing technology to solve the problem of the lack of intra-specific molecular markers of C.sativa in China.

Odontoblasts are primarily responsible for synthesizing and secreting extracellular matrix proteins,which are crucial for dentinogenesis.Our previous single-cell profile and RNAscope for odontoblast lineage revealed that cyclic adenosine monophosphate responsive element-binding protein 3 like 1(Creb3l1)was specifically enriched in the terminal differentiated odontoblasts.In this study,deletion of Creb3l1 in the Wnt1+lineage led to insufficient root elongation and dentin deposition.Assay for transposase-accessible chromatin with high-throughput sequencing(ATAC-seq)and RNA sequencing were performed to revealed that in CREB3L1-deficient mouse dental papilla cells(mDPCs),the genes near the closed chromatin regions were mainly associated with mesenchymal development and the downregulated genes were primarily related to biological processes including cell differentiation,protein biosynthesis and transport,all of which were evidenced by a diminished ability of odontoblastic differentiation,a significant reduction in intracellular proteins,and an even greater decline in extracellular supernatant proteins.Dentin matrix protein 1(Dmp1),dentin sialophosphoprotein(Dspp),and transmembrane protein 30B(Tmem30b)were identified as direct transcriptional regulatory targets.TMEM30B was intensively expressed in the differentiated odontoblasts,and exhibited a significant decline in both CREB3L1-deficient odontoblasts in vivo and in vitro.Deletion of Tmem30b impaired the ability of odontoblastic differentiation,protein synthesis,and protein secretion in mDPCs.Moreover,overexpressing TMEM30B in CREB3L1-deficient mDPCs partially rescued the extracellular proteins secretion.Collectively,our findings suggest that CREB3L1 participates in dentinogenesis and facilitates odontoblastic differentiation by directly enhancing the transcription of Dmp1,Dspp,and other differentiation-related genes and indirectly promoting protein secretion partially via TMEM30B.

Odontoblasts are primarily responsible for synthesizing and secreting extracellular matrix proteins,which are crucial for dentinogenesis.Our previous single-cell profile and RNAscope for odontoblast lineage revealed that cyclic adenosine monophosphate responsive element-binding protein 3 like 1(Creb3l1)was specifically enriched in the terminal differentiated odontoblasts.In this study,deletion of Creb3l1 in the Wnt1+lineage led to insufficient root elongation and dentin deposition.Assay for transposase-accessible chromatin with high-throughput sequencing(ATAC-seq)and RNA sequencing were performed to revealed that in CREB3L1-deficient mouse dental papilla cells(mDPCs),the genes near the closed chromatin regions were mainly associated with mesenchymal development and the downregulated genes were primarily related to biological processes including cell differentiation,protein biosynthesis and transport,all of which were evidenced by a diminished ability of odontoblastic differentiation,a significant reduction in intracellular proteins,and an even greater decline in extracellular supernatant proteins.Dentin matrix protein 1(Dmp1),dentin sialophosphoprotein(Dspp),and transmembrane protein 30B(Tmem30b)were identified as direct transcriptional regulatory targets.TMEM30B was intensively expressed in the differentiated odontoblasts,and exhibited a significant decline in both CREB3L1-deficient odontoblasts in vivo and in vitro.Deletion of Tmem30b impaired the ability of odontoblastic differentiation,protein synthesis,and protein secretion in mDPCs.Moreover,overexpressing TMEM30B in CREB3L1-deficient mDPCs partially rescued the extracellular proteins secretion.Collectively,our findings suggest that CREB3L1 participates in dentinogenesis and facilitates odontoblastic differentiation by directly enhancing the transcription of Dmp1,Dspp,and other differentiation-related genes and indirectly promoting protein secretion partially via TMEM30B.

Objective:To investigate the therapeutic effect of continuous blood purification (CBP) on acute pancreatitis (AP) and its influence on prognosis.Methods:200 patients with AP in our hospital from January 2010 to December 2016 were selected as the subjects,and they were divided into conventional treatment group and CBP treatment group according to the random number table method,600 cases in each group.The conventional treatment group was received conventional drug therapy,and the CBP treatment group was treated with CBP on the basis of commonly used drugs.The disappeared time of clinical symptoms after treatment and the changes of inflammatory factors and the changes of intestinal function before and 72 h after treatment were compared between the two groups,the mortality rate was compared between the two groups at 7 d after treatment.Results:Abdominal pain disappeared time,abdominal distension disappeared time and abdominal tenderness disappeared time in CBP treatment group after treatment were lower than the conventional treatment group (P＜0.05).There was no significant difference in the levels of endotoxin,C reactive protein (CRP),amylase (AMS),two amine oxidase and malondialdehyde before treatment in the two groups (P＞0.05).At 72 h after treatment,endotoxin,CRP,AMS,two amine oxidase and malondialdehyde levels were lower than those before treatment,and the CBP treatment group was lower than the conventional treatment group (P＜0.05).The mortality rate of CBP treatment group was lower than that of conventional treatment group at 7 d after treatment,the difference was statistically significant (P＜0.05).Conclusion:CBP can effectively improve the clinical therapeutic effect of AP,and improve the clinical prognosis of patients.

Objective To explore the influence of introducing standardized patient (SP) into problem-based learning (PBL) to the autonomous learning abilities for undergraduate nursing students based on clinical thinking training. Methods To select 2 classes randomly from full-time professional nursing undergraduate classes enrolled in 2012 from Tianjin university of traditional Chinese medicine, was divided into the experimental group and the control group by random number table method. The control group uses PBL teaching mode, and the experimental group adopts introducing SP into PBL teaching mode. Course lasts for 4 weeks, a total of 18 hours. To evaluate two groups nursing students′the percentage of asking questions by themselves and examination results in the end of the course, and evaluate nursing students′autonomous learning ability before and after the course and after three months of clinical practice respectively. Results The percentage of asking questions by themselves and examination results of the experimental group were 87.80%(36/41) and (84.24±5.12) points, (87.85±5.44) points, the control group were 68.89%(31/45) and (81.51±6.63) points, (84.40±7.96) points, the difference was statistically significant (χ2=4.46, t=2.13, 2.37, P<0.05);There was no statistical significance between two groups in the score of autonomous learning ability before the course (P>0.05), while after course and after three months of clinical practice of the experimental group were (93.71±9.56) points, (103.27±11.92) points, the control group were (85.47 ± 9.28) points , (91.36 ± 10.56) points, the difference was statistically significant (t=3.56, 4.89, P<0.01). Conclusions The teaching mode of introducing SP into PBL can train and improve the autonomous learning ability of undergraduate nursing students,and can provide reference for nursing teaching mode reform.

Objective To investigate the level and influencing factors of subjective well-being of nurses and establish a model of the relationship of job stress, self-efficacy and subjective well-being. Methods Totally 438 nurses from 5 hospitals in Changchun city were investigated with the self-designed demographic questionnaire, General Well-Being Scale, Nurse Job Stressors Scale and Self-Efficacy Scale. Results The total score of subjective well-being was (71.36 ± 18.83) points. Single factor analysis found that age (F=1.759, P<0.01), department (F=1.712, P<0.01), professional title (F=1.364, P<0.05), years of working (F=1.420, P<0.05) and monthly income (F=1.632, P<0.01) were statistically significant. Multiple linear regression analysis showed that sex (t=-2.447, P<0.05), the first factor of job stress (t=-5.033, P<0.01), the second factor (t=-2.478, P<0.05), the third factor (t=-2.315, P<0.05), the fifth factor (t=-5.862, P<0.01) and self-efficacy (t=10.705, P<0.01) were the predictive effectton of subjective well-being. The result of path analysis shows that self-efficacy has a significant effect of adjustment on job stress and subjective well-being (direct effects=-0.62, indirect effects=-0.24, P<0.01). Conclusions The nurse subjective well-being is in the medium level. Hospitals and all related departments should take effective measures to reduce or eliminate unfavorable factors and increase the protective factors, and then improve the level of nurses′subjective well-being and nursing quality.

Objective In order to analyze the research situation,development tendency and deficiencies in the aspects of nursing competency through investigating about 10 kinds of core nursing periodicals published from the year 2009 to 2013 in China.Methods Using bibliometrics methods to analyze the researches about nursing competency in the periodical literature including Chinese Journal of Nursing,Journal of Advanced Study for Nurses,Chinese Journal of Practical Nursing,Chinese Nursing Research,Journal of Nursing Science,Journal of Nursing Administration,Nursing Journal of Chinese People's Liberation Army,Chinese Nursing Management,Shanghai Nursing,Modern Clinical Nursing published from 2009 to 2013.Results There were 215 research papers about nursing competency in periodicals in the past five years,and a quantity of papers increased stably each year.The study contents mainly involved nursing education,training,competency model and standard system based on nursing competency and so on.Conclusions The researches on nursing competency are getting started in China.The quantity is keeping increase and the related research contents become more colorful and deeply than before.