Objective To investigate the effects of microgravity environment on hibernating myoblasts.Methods Hibernating myoblasts were cultured under real and simulated microgravity conditions for 10 days.RNA-seq analysis and immunofluorescence are used to analysis the impact of microgravity environment on cell growth and gene expression of myoblasts.Results Under the microgravity conditions,genes associated with proliferation were upregulated.Under simulated microgravity,there were more and higher proportion of Ki67 positive cells compared to normal gravity conditions.Conclusion The microgravity environment promotes the proliferation of hibernating myoblasts.

Platelet-rich plasma (PRP), the concentration of platelet and plasma, is rich of a variety of growth factors.It plays an important role in anti-inflammatory and tissue regeneration, and has gradually been used in cosmetology in the recent years.This paper introduces the application of PRP in facial rejuvenation, acne and alopecia treatment, as well as improving the survival rate of autologous fat transplantation. The side effects and prospectives of PRP application in cosmetology were also summarized.

Glaucoma, alias'the thief of light', characterized by visual field defects that correspond to damaged areas of the optic nerve head, is a common ophthalmopathy causing irreversible blindness. Thus early diagnosis is crucial. Structural assessment can detect Glaucoma in early stage and diminish the risk of irreversible visual impairment. There are now several apparatus which can clinically measure ONH parameters and confirm DDLS (disc damage likelihood scale) precisely and reproducibly. Comprehensive estimate considering RNFLD (retina nervefiberlayer defects), offers reliable evidence as well as potent support for preclinical detecting, measurement and follow-up observations.

Objective To evaluate the clinical efficacy of antibiotic -impregnated shunt catheters in hydrocephalus shunt. Methods A retrospective analysis of clinical data from patients with hydrocephalus shunt surgery in our hospital during the period from May 2011 to August 2016. Based on the types of catheters, patients were divided into common conduit (not AISCs group) and antibiotics infiltrating pipe groups (AISCs group). All patients were followed up for 6 months after shunt surgery. The diversion and infection was recorded. Results The data of 193 patients with hydrocephalus shunt were analyzed, including 124 cases of non-AISCs group and 69 cases of AISCs group. Compared with non-AISCs group,the incidence of postoperative infection of AISCs was significantly decreased(P<0.05). There was no significant difference in the incidence of shunt and reoperation between the two groups (P>0.05). In addition, there was no significant difference in the rupture, displacement and exposure of the two groups (P>0.05).Conclusion ASICs can effectively reduce the incidence of hydrocephalus shunt infection.

Objective To investigate the risk factors of the mild cognitive impairment(Mild Cognitive Impair?ment,MCI)secondary to the mild to moderate traumatic brain injury patients. Method The clinical and neuroimaging da?ta from 106 patients with mild to moderate traumatic brain injury during July 1, 2014 to July 1 ,2015 were collected. Cog?nitive function in patients with head injury after three months was assessed using the Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), Addenbrooke improved cognitive assessment (ACE-R). The impact of the different lesions on cognitive function was analyzed using univariate and multivariate logistic regression with patients with secondary MCI as the dependent variable and brain tissue lesions as independent variables. Results Sixty-nine pa?tients had normal cognitive function whereas 30 patients had MCI. seven patients were lost to follow. Univariate logistic regression analysis did not revealed any significance in age and GCS scores (P>0.05), but revealed significances in multi?ple lesions, lesioned hemisphere, the lobar white matter lesions and the internal capsule involvement (P<0.05). Multivari?ate logistic regression analysis showed that the left hemisphere(P=0.029,OR=1.637,95%CI=1.348~2.169), temporal lobe area(P<0.001,OR=1.521,95%CI=1.240~2.203), anterior limb(P=0.024,OR=1.526,95%CI=1.107~2.329), multi? ple lesions(P<0.001,OR=1.936,95%CI=1.287~3.228)were the risk factors of MCI. Conclusion Lesions in the left hemisphere, bilateral frontal and temporal lobe area, internal capsule injury and multiple lesions damage are the risk fac?tors of the mild cognitive impairment secondary to the mild to moderate traumatic brain injury.