Objective:To assess the correlation and consistency of electrochemiluminescence and direct chemiluminescence in detecting 12 tumor markers.Methods:A total of 2426 serum specimens were selected from the physical examination in Beijing Tiantan Hospital,Capital Medical University from March to August 2023.These specimens included 446 cases for alpha fetoprotein(AFP),284 cases for carcinoembryonic antigen(CEA),289 cases for carbohydrate antigen 72-4(CA72-4),87 cases for carbohydrate antigen 19-9(CA19-9),205 cases for carbohydrate antigen 125(CA-125),216 cases for carbohydrate antigen 15-3(CA 15-3),292 cases for total prostate specific antigen(TPSA),292 cases for free prostate specific antigen(FPSA),84 cases for serum cytokeratin 19 fragment(Cyfra21-1),84 cases for neuron specific enolase(NSE),84 cases for squamous cell carcinoma associated antigen(SCC)and 63 cases for pro-gastrin-releasing peptide(PROGrp).The electrochemiluminescence and direct chemiluminescence methods were respectively used to detect the above 12 indexes,and then,the correlation and consistency between the two detection methods were analyzed.Results:The results of Pearson and Spearman correlation analysis showed that there were significantly positive correlations between electrochemiluminescence and direct chemiluminescence methods in detecting 12 tumor indexes(AFP,CEA,CA72-4,CA19-9,CA125,CA15-3,TPSA,FPSA,Cyfra21-1,NSE,SCC and PROGrp)(r=0.971,0.934,0.945,0.975,0.900,0.948,0.994,0.984,0.982,0.828,0.879,0.922,P<0.05),respectively.The total coincidence rates between the two methods were respectively 98.21%,98.24%,98.27%,98.85%,97.07%,99.54%,99.66%,99.32%,92.86%,92.86%,95.24%and 96.83%.There were consistencies between electrochemiluminescence and direct chemiluminescence methods for 10 indexes excepted CA15-3 and NSE that could not calculate Kappa values due to data reasons(Kappa=0.848,0.728,0.930,0.794,0.485,0.887,0.664,0.540,0.477,0.652,P<0.05),respectively.Conclusion:In the detections of electrochemiluminescence and direct chemiluminescence methods for tumor indexes,there are favorable correlations and consistencies between them in detecting AFP,CA72-4,CA19-9 and TPSA,and there are favorable correlations between them in detecting CEA,CA125,FPSA,Cyfra21-1,SCC and PROGrp but the consistencies between them are average in detecting these indexes,and there are favorable correlations between them in detecting CA15-3 and NSE.Clinical detection should pay attention to there may be differences in the results between different detection methods when the detection is conducted in reference laboratory.

Objective To retrospectively analyze the characteristics of viral antibodies related to nervous system infections in patients with cognitive impairment,evaluate their relationship with cognitive impairment,and explore the relationship between viral infections and the occurrence and development of cognitive impair-ment.Methods A total of 170 mild cognitive impairment patients who visited Beijing Tiantan Hospital,Cap-ital Medical University from June 2019 to June 2023 were selected as the mild cognitive impairment group,208 cognitive impairment patients were selected as the cognitive impairment group,143 dementia patients were se-lected as the dementia group,and 146 age-matched and gender-matched non infectious and non inflammatory disease controls during the same period in the hospital were selected as the control group.The levels of virus antibodies related to nervous system infections in the serum and cerebrospinal fluid of patients were detected,the detection rate and levels of antibodies were compared,and the infection burden was calculated.The cogni-tive function of all patients was evaluated and the relationship between infection burden and the degree of cog-nitive dysfunction was analyzed.Results The detection rate and antibody level of herpes simplex virus(HSV)1 IgG in the serum of three groups of patients with cognitive impairment were significantly higher than those in the control group(P＜0.05).The detection rate of HSV(1+2)IgM in the serum of three groups of pa-tients with cognitive impairment was higher than that in the control group(P＜0.05),but there was no sta-tistically significant difference between the mild cognitive impairment group,cognitive impairment group,and dementia group(P＞0.05).The serum infection load of three groups of patients with cognitive impairment was higher than that of the control group(P＜0.05),and the higher the infection load,the more severe the cognitive impairment.Conclusion Viral infection is an important influencing factor of cognitive impairment diseases,and HSV infection may be a promoting factor of cognitive dysfunction.