This paper analyzed the clinical data of a patient with recurrent infection of chlamydia psittaci pneumonia within 7 months. The patient had a clear history of contact with live poultry, and the clinical manifestations were dry cough, persistent fever, and respiratory failure. Chest CT imaging changes showed lobar consolidation of infected lung lobes, ground-glass shadows, bronchial air-filling signs, and pleural effusion. The two infections were detected in bronchoalveolar lavage fluid by metagenomic next-generation sequencing (mNGS) and pathogen targeted next-generation sequencing (tNGS), respectively, to achieve early diagnosis of chlamydia psittaci pneumonia. New tetracycline drugs were used as the core of treatment for both infections, and rapid improvement was achieved after anti-infection treatment.

This paper analyzed the clinical data of a patient with recurrent infection of chlamydia psittaci pneumonia within 7 months. The patient had a clear history of contact with live poultry, and the clinical manifestations were dry cough, persistent fever, and respiratory failure. Chest CT imaging changes showed lobar consolidation of infected lung lobes, ground-glass shadows, bronchial air-filling signs, and pleural effusion. The two infections were detected in bronchoalveolar lavage fluid by metagenomic next-generation sequencing (mNGS) and pathogen targeted next-generation sequencing (tNGS), respectively, to achieve early diagnosis of chlamydia psittaci pneumonia. New tetracycline drugs were used as the core of treatment for both infections, and rapid improvement was achieved after anti-infection treatment.

Objective To explore the underlying molecular mechanism of quercetin in tuberculous ulcer treatment using network phar-macology and molecular docking.Methods We identified quercetin drug targets by searching the PubChem,SwissTarget,and TargetNet databases,then combining our results with those of previous tuberculosis ulcer gene sequencing in our group,thereby obtaining inter-section targets.Using the DAVID database,we performed intersection target gene ontology functional enrichment analysis and signaling pathway enrichment analysis of the Kyoto gene and genome database.We analyzed the intersection target using the STRING database and Cytoscape software and screened the hub node.We used PyMOL and AutoDockTolls software to complete quercetin molecular docking with the hub node,then screened the core drug target of quercetin.Finally,we constructed a macrophage model to verify the above-men-tioned core genes.Results We screened overall 54 drug targets.Our enrichment analysis indicated that the signaling pathways involved in quercetin-mediated tuberculous ulcer treatment were e.g.,metabolic pathways,lipid and atherosclerosis,or the MAPK signaling pathway.In addition,ALOX5,TNF,SRC,MMP9,and EGFRmight be the key genes in quercetin-mediated tuberculous ulcer treatment.Results of our cell culture experiment demonstrated that upon quercetin intervention,SRCand EGFRexpression increased significantly while that of MMP9decreased significantly in M1 and M2 macrophages.Conclusion Quercetin could potentially regulate macrophage polarization by influencing SRC,EGFR,and MMP9expression.

Objective To retrieve, evaluate, and summarize evidence on preventive management of high output and dehydration in ileostomy patients from both domestic and international sources. Methods Based on the "6S" evidence model, a top-down approach was employed to retrieve evidence on prevention and management of high output and dehydration in ileostomy patients. Databases searched included BMJ best practice, UpToDate, National Guideline Clearinghouse (NGC), National Institute for Health and Care Excellence (NICE), Scottish Intercollegiate Guidelines Network (SIGN), Registered Nurses' Association of Ontario (RNAO), Wound Ostomy and Continence Nurses Society (WOCNS), World Council of Enterostomal Therapists (WCET), Medlive Clinical Guidelines website, Joanna Briggs Institute (JBI), Cochrane Library, Embase, PubMed, Web of Science, CNKI, Wanfang Data, China Biology Medicine (CBM), and VIP Database. The search period was from January 2019 to April 2024. Results A total of 13 articles were included after retrieval and screening, comprising 2 guidelines, 2 expert consensuses, 4 systematic reviews, 4 evidence summaries, and 1 clinical decision aid. After translation, summarization, and organization of the included articles, 19 pieces of evidence across 11 categories were formulated, focusing on four items: definition and risk factors of high output in ileostomy, prevention and management strategies related to high output in ileostomy, prevention and management strategies related to dehydration in high-output ileostomy, and follow-up strategies for prevention of high output and dehydration after ileostomy surgery. Conclusion The evidence summarized based on the "6S" evidence model for prevention and management of high output and dehydration in ileostomy patients can provide a reference for clinical practice among healthcare professionals and medical decision-makers, thereby enhancing nursing quality and reducing patient rehospitalization rates.

[Abstract]? Objective? To explore medical staff's attitude towards in-hospital treatment mode among patients with acute ischemic stroke(AIS),and the optimizable factors of the mode,so as to provide reference for further optimization of the treatment mode. Methods? From November of 2017 to January of 2018, 15 medical staff from the AIS team in Changhai Hospital Affiliated to the Second Military Medical University were selected by purposive sampling and interviewed by semi-structured interviewing method. Data was collected in the way of handwriting and recording. Colaizzi data analysis was used to analyze the materials. Results? The experience of medical staff in optimizing the treatment mode of AIS hospital included five themes: (1)the treatment mode of AIS hospital was basically reasonable; (2)the need for early warning of neurointerventional treatment team;(3)the need for the construction of specialist nursing team for the emergency treatment of stroke; (4)the need for supervision of the treatment process of AIS by information technology; (5)the need for pre-hospital warning. Conclusions? It is necessary to make preparations in advance for invasive treatment team, construct the specialist stroke first aid nursing team for the whole process guiding and quality control, promote the integration of pre-hospital and in-hospital treatment so as to activate the in-hospital treatment system as soon as possible, improve the construction of AIS treatment informatization in order to assist the supervision of treatment efficiency, and further shorten the time of in-hospital treatment of AIS.

"5+3" integrated training program is a new training model to cultivate doctors under the background of preclinical teaching and clinical training integration. Based on practice in Shantou University Medical College, the program design current progress and solution to the problem were elaborated. It adheres to principles of overall optimization and orienting to clinic, and to ensure the smooth implementation of the training model. Which will provide important reference for the reform of long-term medical training program in higher medical colleges and universities.

Objective To observe the protein expression , subcelluar localization of activated Pak 1 and its relation-ship with chromosome separation during meiosis in mouse oocytes .Methods Western blot was applied to analyze the expression of activated Pak1 (phosphorylated Pak1 at Ser204, pPak1Ser204 ) at different stages of meiotic progres-sion in semi-quantitative manner;immunofluorescent staining was employed to detect the sub-cellular localization of pPak1Ser204 and its spatial-temporal correlation with spindle and microtubule organizing centers (MTOC) during oo-cyte meiosis.Results pPak1Ser204 expression was found upon germinal vesicle breakdown (GVBD) in mouse oo-cytes which increased along with the meiotic progression , reaching peak level at metaphase Ⅰ( MⅠ) , which re-mained up to meiosis Ⅱ(MⅡ).From pro-metaphase Ⅰ(pro-MⅠ) to MⅠand at MⅡstage, pPak1Ser204was co-localized with MTOC key components , pericentrin and γ-tubulin on spindle poles, pPak1Ser204 was also distributed on chromosomes;During anaphase Ⅰ(AⅠ) to telophase Ⅰ(TelⅠ) progression, pPak1Ser204 was detached from chromosomes and MTOC, and mainly concentrated on the cleavage furrow.Conclusions pPak1Ser204 is expressed upon meiotic resumption in oocytes .It is a MTOC-associated protein which may regulate chromosome separation through participating the formation and maintenance of spindle apparatus .