Objective:To review and summarize the experience of robot-assisted laparoscopic trans-plant nephrectomy,share the surgical steps and technical key points,and provide a reference for clinical practice.Methods:A retrospective analysis was conducted on the perioperative data of 5 patients who underwent robot-assisted laparoscopic donor nephrectomy at Peking University Third Hospital from August 2023 to December 2024.The surgical steps and key points were summarized.The continuous variables were described by medians(ranges).Results:A total of 5 patients were included in the analysis,of whom 2 were male and 3 were female.The median age of the patients was 37(31-68)years.The me-dian time from kidney transplantation to donor nephrectomy was 10(3-22)years.The indications for donor nephrectomy included recurrent hematuria,abdominal pain,malignant tumor of the transplanted kidney,and recurrent infection with hydronephrosis of the transplanted kidney.The excised transplanted kidneys from all the 5 patients had a single renal artery and a single renal vein.The median operation time was 212(145-351)min,the median blood loss was 300(20-500)mL,and the median post-operative hospital stay was 7(4-25)days.Only 1 patient experienced intraoperative complications,who experienced an external iliac artery injury during the operation and underwent suture repair.No pa-tient died during the perioperative period.Postoperative pathological results showed that 3 patients had end-stage non-functional kidneys,1 patient had BK virus-associated urothelial carcinoma,and 1 patient had chronic pyelonephritis with renal parenchymal atrophy.Conclusion:Robot-assisted laparoscopic transplant nephrectomy as a new surgical approach is feasible and safe.Compared with traditional open transplant nephrectomy,its advantage lies in the ability to directly observe and prioritize the management of the renal pedicle of the transplanted kidney,while completely freeing and removing the transplanted kidney outside the renal capsule.With the continuous accumulation of experience,this surgical technique is expected to become a powerful alternative to traditional open transplant nephrectomy.

Objective:To review and summarize the experience of robot-assisted laparoscopic trans-plant nephrectomy,share the surgical steps and technical key points,and provide a reference for clinical practice.Methods:A retrospective analysis was conducted on the perioperative data of 5 patients who underwent robot-assisted laparoscopic donor nephrectomy at Peking University Third Hospital from August 2023 to December 2024.The surgical steps and key points were summarized.The continuous variables were described by medians(ranges).Results:A total of 5 patients were included in the analysis,of whom 2 were male and 3 were female.The median age of the patients was 37(31-68)years.The me-dian time from kidney transplantation to donor nephrectomy was 10(3-22)years.The indications for donor nephrectomy included recurrent hematuria,abdominal pain,malignant tumor of the transplanted kidney,and recurrent infection with hydronephrosis of the transplanted kidney.The excised transplanted kidneys from all the 5 patients had a single renal artery and a single renal vein.The median operation time was 212(145-351)min,the median blood loss was 300(20-500)mL,and the median post-operative hospital stay was 7(4-25)days.Only 1 patient experienced intraoperative complications,who experienced an external iliac artery injury during the operation and underwent suture repair.No pa-tient died during the perioperative period.Postoperative pathological results showed that 3 patients had end-stage non-functional kidneys,1 patient had BK virus-associated urothelial carcinoma,and 1 patient had chronic pyelonephritis with renal parenchymal atrophy.Conclusion:Robot-assisted laparoscopic transplant nephrectomy as a new surgical approach is feasible and safe.Compared with traditional open transplant nephrectomy,its advantage lies in the ability to directly observe and prioritize the management of the renal pedicle of the transplanted kidney,while completely freeing and removing the transplanted kidney outside the renal capsule.With the continuous accumulation of experience,this surgical technique is expected to become a powerful alternative to traditional open transplant nephrectomy.

The historical evolution of the fire-processing of Aconiti Lateralis Radix Praeparata(Fuzi)was explored by literature research and the re-practice of ancient processing method.The literature research showed that the Han Dynasty was the beginning of fire-processing of Fuzi,and the processing techniques of Fuzi varied.During the Jin and Tang Dynasties,ash fire was preferred for fire-processing techniques;in the Song Dynasty,the concept of fully-processing and the method of macerating Fuzi with liquid before fire-processing appeared;in the Jin and Yuan Dynasties,the processing criterion of presenting yellow color inside and outside and the remedial methods after fire-processing were put forward;in the Ming Dynasty,the techniques of water-macerating,fire-processing,slicing and baking became the mainstream,and the Qing Dynasty further refined the techniques in terms of process details.After the founding of the People's Republic of China,the sand-scalding technique developed from the local processing experiences and standards has disengaged from the ancient methods.The literature evidence and the re-practice of ancient processing method revealed that the traditional fire-processing techniques on Fuli such as ash-fire-processing,water-macerating before fire-processing,and quenching after fire-processing,are all about the control of temperature and processing time and the criterion of presenting yellow color inside and outside.For the preparation of Fuzi standard in the formulas containing Fuzi,it is recommended to process Fuzi at the temperature of simulated hot ashes in an oven,and the standard should be uniformly yellow after processing.

Objective This study investigated feasible cases and their significance in promoting the"generalized"development of inspection through digital-intelligent equipment.Methods A qualitative research approach was used,involving interviews conducted between February 2025 and March 2025 with experts in traditional Chinese medicine diagnostics,clinical research methodology,medical engineering integration,and related disciplines,using both online and offline methods.In accordance with the Consolidated Criteria for Reporting Qualitative Research,feasible cases involving the specific application of digital equipment in various parts of observation were collected through item enrichment.The significance of extending observation capabilities via these cases was analyzed,along with the overall implications of integrating digital technologies with traditional inspection method.Results Interviews were completed with 11 experts from domestic universities and research institutes in the fields of traditional Chinese medicine diagnosis,medical engineering integration,and related disciplines.A total of 78 feasible cases of digital-intelligent inspection were identified,along with 69 insights regarding the significance of enhancing the inspection capabilities.These insights were synthesized into two dimensions and 23 holistic meanings.The first dimension is to expand the scope of inspection,including obtaining internal environmental characteristics,observing external environmental characteristics,expanding thermodynamic characteristic data,and crossing time and space.The second dimension is to improve the quality of observation and diagnosis information collection and analysis,including 19 specific meanings,such as standardized collection environment,objective quantification,and refined observation.Conclusion Digital-intelligent equipment plays a significant role in expanding the scope of inspection content and achieving high-quality acquisition and analysis of extensive inspection information.These advancements extend and enrich the capabilities of traditional inspection method in traditional Chinese medicine.

Objective This study investigated feasible cases and their significance in promoting the"generalized"development of inspection through digital-intelligent equipment.Methods A qualitative research approach was used,involving interviews conducted between February 2025 and March 2025 with experts in traditional Chinese medicine diagnostics,clinical research methodology,medical engineering integration,and related disciplines,using both online and offline methods.In accordance with the Consolidated Criteria for Reporting Qualitative Research,feasible cases involving the specific application of digital equipment in various parts of observation were collected through item enrichment.The significance of extending observation capabilities via these cases was analyzed,along with the overall implications of integrating digital technologies with traditional inspection method.Results Interviews were completed with 11 experts from domestic universities and research institutes in the fields of traditional Chinese medicine diagnosis,medical engineering integration,and related disciplines.A total of 78 feasible cases of digital-intelligent inspection were identified,along with 69 insights regarding the significance of enhancing the inspection capabilities.These insights were synthesized into two dimensions and 23 holistic meanings.The first dimension is to expand the scope of inspection,including obtaining internal environmental characteristics,observing external environmental characteristics,expanding thermodynamic characteristic data,and crossing time and space.The second dimension is to improve the quality of observation and diagnosis information collection and analysis,including 19 specific meanings,such as standardized collection environment,objective quantification,and refined observation.Conclusion Digital-intelligent equipment plays a significant role in expanding the scope of inspection content and achieving high-quality acquisition and analysis of extensive inspection information.These advancements extend and enrich the capabilities of traditional inspection method in traditional Chinese medicine.

[Objective]To explore the traditional processing techniques and Jingbang(Beijing local)experience of Ophiopogon japonicus,providing a reference for modern research on Opehiopogon japonicus.[Methods]Collecting data on the processing of Ophiopogon japonicus from ancient herbal literature and traditional Jingbang heritage materials,and consulting with old pharmacists,based on the analysis and organization,the various processing methods of Ophiopogon japonicus were restored.[Results]The purification methods of Ophiopogon japonicus have mechanisms such as removing the pith to relieve annoyance,removing the pith to treat channel dryness,and removing the pith to nourish lung Yin,with the first one being the most widely spread.The methods of removing the pith include boiling water to moisten the pith,clear water to moisten the pith,and roasting to soften the pith,with the first one being the most effective.The purpose of the wine soaking method is to alleviate the side effects of long-term use of Ophiopogon japonicus on stomach cold.The rice frying method should spread the rice thinly in the pot,spread Ophiopogon japonicus on the rice,and should not be stirred.If it is stirred with the rice,Ophiopogon japonicus is easy to puff up.The steaming method has two types:clear steaming and mixing with milk steaming.Ophiopogon japonicus is not easy to crush,and the intermittent roasting method recorded in Compendium of Materia Medica is very good.The method of simmering Ophiopogon japonicus and making Ophiopogon pills both utilize its juice-containing characteristics.The traditional Jingbang Ophiopogon japonicus has obvious local characteristics in the methods of moistening and removing the pith.Zhu Ophiopogon japonicus is for sprinkling water and moistening slightly,and cinnabar fine powder is finely scattered with sprinkling and stirring.These two processed varieties have disappeared for half a century.[Conclusion]The restoration and optimization of the processing methods of Ophiopogon japonicus are expected to improve the efficacy of Ophiopogon japonicus,providing higher quality Ophiopogon japonicus medicinal materials for the clinic.It reveals the local characteristics of the traditional Jingbang Ophiopogon japonicus processing,providing a possibility for the revival of the disappeared processed varieties,which is of great significance for enriching Chinese medicine resources and enhancing the clinical application value of Chinese medicine.

Objective To investigate the clinical application value of ¹⁸F-FDG PET-CT simulation localization in radiotherapy of recurrent abdominal and pelvic tumors. Methods ¹⁸F-FDG PET-CT was used to simulate positioning 38 patients with abdominal and pelvic tumors who relapsed after treatment.Based on both CT images and ¹⁸F-FDG PRT-CT, we drew up a systemic treatment plan and outlined the radiotherapy target area, and then compared the differences between the two methods. Results In 38 patients, 21.1%(3/8) of patients were found to have distal metastases outside the pelvic and abdominal cavity, and changed the systemic treatment plan.The radiotherapy target was altered in 34(89.5%) patients.The mean value of GTV_PET-CT was 118.14cm³and the mean value of GTV_CT was 148.53cm³(P=0.044). Conclusion For patients with recurrent abdominal and pelvic tumors, ¹⁸F-FDG PET-CT simulation localization treatment improves tumor re-staging, changes the integrated therapy for some patients, and makes the target area of radiotherapy more accurate.

Objective To discuss the clinical effect of horn perforator flaps for the treatment of finger soft tissue defect.Methods ① Lead oxide-gelatin mixture injection and CT scanning were performed in 4 fresh adult cadavers and the data was put into mimics 17.0 to achieve the three-dimensional reconstruction.The regulation and origins of the perforator vessel in proper palmar digital artery was observed.②From July 2012 to July 2015,9 cases with finger soft tissue defects were treated with horn perforator flaps.The defect areas ranged from 0.6 cm × 0.8 cm to 1.2 cm × 1.5 cm and the flap size ranged from0.8 cm×2.5 cm to 1.6 cm×4.2 cm.We put the head end of flaps at defect side.The horn perforator flap was designed with 1-2 perforator vessel and rotated to cover the defect.The defect at donor site was closed directly.Results We got 4 symmetric arteries in palmar and back surfaces of fingers,2 for proper palmar digital arteries and 2 for dorsal digital arteries.The proper palmar digital arteries have a larger diameter which is the main source of blood supply for fingers.All 9 flaps survived after the operation.Both the recipient area and donor areas received sound healing.With 2 months to 1.5 years of follow-up visit,the shape,color and luster,texture and function all had good healing.Conclusions The horn perforator flaps for repair finger soft tissue defect have advantages of flexible design,reliable perforator vessels.The defect at donor site can be closed directly with no skin graft.This method considers both shape and function and is a good way for finger soft tissue defect.

Objective To discuss the clinical effect of horn perforator flaps for the treatment of finger soft tissue defect.Methods ① Lead oxide-gelatin mixture injection and CT scanning were performed in 4 fresh adult cadavers and the data was put into mimics 17.0 to achieve the three-dimensional reconstruction.The regulation and origins of the perforator vessel in proper palmar digital artery was observed.②From July 2012 to July 2015,9 cases with finger soft tissue defects were treated with horn perforator flaps.The defect areas ranged from 0.6 cm × 0.8 cm to 1.2 cm × 1.5 cm and the flap size ranged from0.8 cm×2.5 cm to 1.6 cm×4.2 cm.We put the head end of flaps at defect side.The horn perforator flap was designed with 1-2 perforator vessel and rotated to cover the defect.The defect at donor site was closed directly.Results We got 4 symmetric arteries in palmar and back surfaces of fingers,2 for proper palmar digital arteries and 2 for dorsal digital arteries.The proper palmar digital arteries have a larger diameter which is the main source of blood supply for fingers.All 9 flaps survived after the operation.Both the recipient area and donor areas received sound healing.With 2 months to 1.5 years of follow-up visit,the shape,color and luster,texture and function all had good healing.Conclusions The horn perforator flaps for repair finger soft tissue defect have advantages of flexible design,reliable perforator vessels.The defect at donor site can be closed directly with no skin graft.This method considers both shape and function and is a good way for finger soft tissue defect.

Amniotic Fluid ; drug effects ; metabolism ; Female ; Fetus ; drug effects ; metabolism ; Gene Expression Regulation, Developmental ; drug effects ; genetics ; Humans ; MicroRNAs ; genetics ; pharmacology ; Placenta ; metabolism ; Pregnancy ; RNA, Plant ; genetics ; pharmacology ; Umbilical Cord ; drug effects ; metabolism