Objective:To explore the recovery time of ovary in patients who underwent all embryo freezing after oocyte retrieval in in vitro fertilization (IVF) and the relationship between pregnancy outcome and embryo transfer time. Methods:This research included 53 patients who underwent all embryos freezing in the Reproductive Hospital of Shandong University from July to December in 2017. The baseline level of serum estradiol in the first menstrual cycle after oocyte retrieval and the ovulation time during the second menstrual cycle after oocyte retrieval was assessed in 53 patients. In addition, the pregnancy outcomes of all patients underwent frozen-thawed embryo transfer were followed up.Results:During the second to the fourth day of the first menstrual cycle after oocyte retrieval, 88.68% (47/53) of the patients had a blood estradiol level below 50 ng/L, which had no relationship with the age of the patients (≤30 years old, >30 years old) ( P=0.161), the method of ovulation induction ( P=0.516), with or without polycystic ovary syndrome ( P=0.661), the number of oocyte obtained (≤15, >15) ( P=0.671). Among patients without polycystic ovary syndrome and regular menstrual cycle, 76.00% (19/25) of patients had recovered ovulation in the second menstrual cycle after oocyte retrieval, and the median ovulation time was the 16th day of menstruation cycle. There was no significant difference in the pregnancy outcomes between patients who had embryo transfer during the second menstrual cycle and patients who had embryo transfer during the third menstrual cycle or later ( P>0.05). Conclusion:It is rational to transfer embryo earlier for patients who underwent all embryo freezing after oocyte retrieval in IVF.

Objective:To explore the recovery time of ovary in patients who underwent all embryo freezing after oocyte retrieval in in vitro fertilization (IVF) and the relationship between pregnancy outcome and embryo transfer time. Methods:This research included 53 patients who underwent all embryos freezing in the Reproductive Hospital of Shandong University from July to December in 2017. The baseline level of serum estradiol in the first menstrual cycle after oocyte retrieval and the ovulation time during the second menstrual cycle after oocyte retrieval was assessed in 53 patients. In addition, the pregnancy outcomes of all patients underwent frozen-thawed embryo transfer were followed up.Results:During the second to the fourth day of the first menstrual cycle after oocyte retrieval, 88.68% (47/53) of the patients had a blood estradiol level below 50 ng/L, which had no relationship with the age of the patients (≤30 years old, >30 years old) ( P=0.161), the method of ovulation induction ( P=0.516), with or without polycystic ovary syndrome ( P=0.661), the number of oocyte obtained (≤15, >15) ( P=0.671). Among patients without polycystic ovary syndrome and regular menstrual cycle, 76.00% (19/25) of patients had recovered ovulation in the second menstrual cycle after oocyte retrieval, and the median ovulation time was the 16th day of menstruation cycle. There was no significant difference in the pregnancy outcomes between patients who had embryo transfer during the second menstrual cycle and patients who had embryo transfer during the third menstrual cycle or later ( P>0.05). Conclusion:It is rational to transfer embryo earlier for patients who underwent all embryo freezing after oocyte retrieval in IVF.

Objective To compare the difference in risks of obstetric complications of singleton pregnancy between women with hyperandrogenic polycystic ovary syndrome (PCOS) and women with normoandrogenic PCOS. Methods Prospective cohort study. This study was a secondary analysis of data collected during a multicenter randomized controlled clinical trial. Women who got clinical singleton pregnancy were grouped according to whether they were diagnosed with hyperandrogenism at baseline. There were 118 women with hyperandrogenism and 366 women without hyperandrogenism. The incidences of obstetric complications and birth weight were compared between the two groups. Results Women with hyperandrogenic PCOS had a significantly higher risk of preterm delivery than women with normoandrogenic PCOS [12.7％ (15/118) versus 3.6％ (13/366); OR=3.94, 95％CI: 1.82-8.56]. After adjustment of age, duration of infertility, body mass index, and fresh or frozen embryo transfer group, hyperandrogenism was still associated with an increased risk of preterm delivery (OR=3.67, 95％CI: 1.67-8.07). Compared with women with normoandrogenic PCOS, women with hyperandrogenic PCOS had similar risks of pregnancy loss, gestational diabetes mellitus, pre-eclampsia, placenta previa, and postpartum hemorrhage (all P>0.05). Birth weight as well as the risks of being small for gestational age and large for gestational age were also comparable between the two groups (all P>0.05). Conclusion In women with PCOS and singleton pregnancy, those with preconceptional hyperandrogenism have a higher risk of preterm delivery than those without hyperandrogenism.

Objective To investigate the relationship between androgen level and body adipose tissue content and distribution via a cross sectional survey in healthy women aged 40 to 60 years. Methods A total of 222 women were divided into 4 groups according menstruation status, i.e. reproductive stage, early perimenopausal stage, late perimenopausal stage and postmenopausal stage. Serum level of dehydroepiandrosterone (DHEA), total testosterone (TT) and sex hormone binding globulin (SHBG) were measured. Free androgen index (FAI) was calculated. Body adipose tissue content and distribution were measured by dual-energy X-ray absorptiometry. Results In women aged 40 to 60 years, DHEA, TT and FAI level of reproductive stage women was (12.3±4.1) nmol/L, (0.56±0.22) nmol/L and 1.15 (quartile:0.71 to 1.85), respectively. DHEA, TT and FAI level of early perimenopausal stage women was (12.0±3.4) nmol/L, (0.56 ± 0.24) nmol/L and 1.37 (quartile: 0.89 to 1.61), respectively. DHEA, TT and FAI level of late perimenopausal stage women was (14.2 ± 4.7) nmol/L, (0.62 ± 0.18) nmol/L and 1.38 (quartile:1.12 to 1.63). DHEA, TT and FAI level of postmenopausal stage women was (11.6±3.5) nmol/L, (0.45±0.22) nmol/L and 0.94 (quartile:0.47 to 1.49). DHEA, TT and FAI level of perimenopausal stage women was comparable with those of reproductive stage women (P>0.05), however, TT and FAI level of postmenopausal women was significantly lower than those of reproductive stage women (P=0.001, 0.014). The total adipose percentage of reproductive stage women, early perimenopausal stage women, late perimenopausal stage women and postmenopausal stage women were (35 ± 6)%, (35 ± 5)%, (37 ± 4)%and (37 ± 5)%. The adipose percentage in“android”area of reproductive stage women, early perimenopausal stage women, late perimenopausal stage women and postmenopausal stage women were (43±5)%, (43±4)%, (47±5)%and (46±5)%. The total adipose percentage was similar in 4 groups (P=0.312). Compared with reproductive stage women, adipose percentage of“android”area increased in late perimenopausal and postmenopausal women (P=0.026). Women with higher FAI level presented higher adipose tissue content and higher percentage of centrally distributed adipose tissue (r=0.28, P=0.003). Conclusions Body adipose tissue tends to distribute centrally from perimenopausal stage. Androgen level is related to body adipose tissue content and distribution, but may not be the main reason of changes of fat distribution in middle life women.

The rate of bone turnover in postmenopausal women accelerates and the newly formed osteoid is poorly mineralized, resulting in the loss of bone mineral content. Meanwhile, the requirement for calcium increases as more bone matrix needs to be mineralized. On the other hand, the reduction of serum estrogen level impairs the absorption of calcium in intestinal tract and the reabsorption in kidney, resulting in the decreased absorption and increased excretion of calcium. Therefore, sufficient calcium intake is critical for maintaining the bone structure in postmenopausal women. The reference intake of calcium differs greatly among different countries. In 2000, China established the adequate intake of calcium for Chinese women aged 50 years and older as 1000 mg/d. Diets provide the optimal source of calcium to prevent osteoporosis. Although calcium supplements have been demonstrated to be beneficial for the bone mineral density in postmenopausal women, its impact on fracture risk and cardiovascular diseases remains controversial. Available evidences suggest that calcium supplements combined with vitamin D are unlikely to increase the risk of cardiovascular diseases.

Objective To evaluate the relationship between endometriosis fertility index (EFI) and pregnancies after laparoscopic surgery in endometriosis-associated infertility.Methods From Jan.2005 to Jan.2010,medical documents of 350 infertile patients due to endometriosis undergoing laparoscopic surgery were studied retrospectively.Pregnancy outcomes were followed up by telephone.EFI was calculated by history factors,least function score and some aspects of the revised American Fertility Society (r-AFS) endometriosis stage.The cumulative pregnancy rate was calculated and compared by Kaplan-Meier survival analysis.Results Within 3 years after surgery,the cumulative pregnancy rates among patients with EFI score 8,9,10 were 62.5％,69.8％ and 81.1％,respectively.There was no significant difference in pregnancy rates among those three groups of patients ( P =0.24 ).The cumulative pregnancy rates among patients with EFI score 5,6,7 were 49.8％,43.9％ and 41.6％,respectively,which did not reach statistical difference ( P =0.83 ).The cumulative pregnancy rates of EFI score 8 - 10 was significantly higher than that of EFI score 5 -7 (71.8％ vs.44.4％,P =0.000).The patients with EFI score 0 -4 was quite small with only 33 cases,among which 15 cases were pregnant.Conclusions There is relationship between EFI and pregnancy in patients with endometriosis-associated infertility.EFI is meaningful to guide post surgical treatment.