Objective:To identify the differentially expressed snoRNAs in the carcinogenesis of cells induced by α-particles radiation and predict the targeted genes and RNA-co-expression networks.Methods:Full transcriptome expression microarray biochips were employed to screen the differentially expressed snoRNAs between human bronchial epithelial BEP2D cell line and its derivative malignantly transformed cell line BERP35T-4 established by α-particle irradiation. The expression changes of snoRNAs and their derived sdRNAs were confirmed by qRT-PCR. The functional domains, targets and co-expression networks of snoRNA were predicted by bioinformatics analysis.Results:Consistent with the result of microarray assay, the expression changes of the screened snoRNAs were confirmed by qRT-PCR. The expressions of sno116 family decreased in BERP35T-4, which was 0.105% ( t=26.60, P<0.01) of BEP2D, and they were generally down-regulated in radiation-induced carcinogenic BERP35T-4 cells and the human lung cancer cell lines A549 and H1299. It was also found that the expression level of the sdRNAs derived from sno116-14 was significantly different in the same cells. It was speculated that these less expressed sdRNAs of sno116-14 could be due to degradation as the consequence of interaction with their targets. The co-expression networks of sno116 family with other types of RNA were established, and the predicted targets of sno116-14 included ZNF280D, TFDP1, CCDC28B, RPS6KA3, CANX, RUNX1 and KALRN, which were related to the functions of cell proliferation and cytoskeletal structure. Conclusions:Some differentially expressed snoRNAs related to α-particle induced carcinogenesis have been identified. It is predicted that the target gene of sno116-14 is involved in the biological processes such as cell proliferation, cytoskeletal structure and the signaling pathways for function regulation, providing new information for the function model of C/D box snoRNAs and the mechanism of radiation carcinogenesis.

Objective To explore the abnormalities of amplitude of low-frequency fluctuation (ALFF) in the resting-state functional magnetic resonance imaging (rs-fMRI) of first-episode medication-naive schizophrenia before and after antipsychotic drugs therapy,the correlation between brain activity and clinical syndromes was to be analyzed.Methods This prospective study was approved by the local ethical conmnittee,and written informed consent was obtained from all participants.21 antipsychotic-naive first-episode patients with schizophrenia and 25 healthy individuals were recruited and underwent resting-state functional MR imaging.The patients were re-scanned at the end of 3 months after the antipsychotic treatment.The differences of ALFF between patients and controls were analyzed with two-sample t test,the differences of ALFF in patients before and after antipsychotic drugs therapy were analyzed with two-sample t test.Results (1) The scores of Global Assessment of Functioning Scale,PANSS total score,PANSS positive score,PANSS negative score and PANSS general psychopathology score were significantly improved after antipsychotic drugs therapy (t=6.45,5.13,3.98,3.29,4.32;P＜0.01).(2) Compared with the healthy controls,resting-state ALFF in schizophrenia patients significantly decreased in left paracentral lobule(PCL),left superior occipital gyrus(SOG)/bilateral calcarine/cuneus,middle occipital gyrus(MOG),and right insula (uncorrected,t=-4.91,-4.30,-4.18,-3.59;P＜0.01,cluster voxels＞10),resting-state ALFF in schizophrenia patients significantly increased in anterior cingulate cortex (ACC) and right lentiform nucleus (uncorrected,t=3.60,3.44;P＜0.01,cluster voxels＞10).(3) After antipsychotic drugs therapy for 3 months,the ALFF values increased in bilateral cuneus and decreased in left ACC compared with baseline(uncorrected,t=3.91,-4.42;P＜0.01,cluster voxels＞10).(4) The ALFF values in the anterior cingulate cortex(ACC) at baseline were positively correlated with negative symptoms scores of PANSS (r=0.594,P=0.005).The ALFF values in the left MOG at baseline were negatively correlated with total scores of PANSS (r=-0.464,P=0.034).Conclusions This study provides evidence for resting state brain activity abnormalities of some brain regions in patients with schizophrenia.ACC and occipital gyrus are the key brain regions and may be responsible for certain schizophrenic symptoms,which could contribute to explore the neuropharmacology mechanisms of schizophrenia.

Objective To explore the abnormalities of amplitude of low-frequency fluctuation (ALFF) in the resting-state functional magnetic resonance imaging (rs-fMRI) of first-episode medication-naive schizophrenia before and after antipsychotic drugs therapy,the correlation between brain activity and clinical syndromes was to be analyzed.Methods This prospective study was approved by the local ethical conmnittee,and written informed consent was obtained from all participants.21 antipsychotic-naive first-episode patients with schizophrenia and 25 healthy individuals were recruited and underwent resting-state functional MR imaging.The patients were re-scanned at the end of 3 months after the antipsychotic treatment.The differences of ALFF between patients and controls were analyzed with two-sample t test,the differences of ALFF in patients before and after antipsychotic drugs therapy were analyzed with two-sample t test.Results (1) The scores of Global Assessment of Functioning Scale,PANSS total score,PANSS positive score,PANSS negative score and PANSS general psychopathology score were significantly improved after antipsychotic drugs therapy (t=6.45,5.13,3.98,3.29,4.32;P＜0.01).(2) Compared with the healthy controls,resting-state ALFF in schizophrenia patients significantly decreased in left paracentral lobule(PCL),left superior occipital gyrus(SOG)/bilateral calcarine/cuneus,middle occipital gyrus(MOG),and right insula (uncorrected,t=-4.91,-4.30,-4.18,-3.59;P＜0.01,cluster voxels＞10),resting-state ALFF in schizophrenia patients significantly increased in anterior cingulate cortex (ACC) and right lentiform nucleus (uncorrected,t=3.60,3.44;P＜0.01,cluster voxels＞10).(3) After antipsychotic drugs therapy for 3 months,the ALFF values increased in bilateral cuneus and decreased in left ACC compared with baseline(uncorrected,t=3.91,-4.42;P＜0.01,cluster voxels＞10).(4) The ALFF values in the anterior cingulate cortex(ACC) at baseline were positively correlated with negative symptoms scores of PANSS (r=0.594,P=0.005).The ALFF values in the left MOG at baseline were negatively correlated with total scores of PANSS (r=-0.464,P=0.034).Conclusions This study provides evidence for resting state brain activity abnormalities of some brain regions in patients with schizophrenia.ACC and occipital gyrus are the key brain regions and may be responsible for certain schizophrenic symptoms,which could contribute to explore the neuropharmacology mechanisms of schizophrenia.