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1.ToxR Is Required for Biofilm Formation and Motility of Vibrio Parahaemolyticus.

Long CHEN ; Yue QIU ; Hao TANG ; Ling Fei HU ; Wen Hui YANG ; Xiao Jue ZHU ; Xin Xiang HUANG ; Tang WANG ; Yi Quan ZHANG

Biomedical and Environmental Sciences 2018;31(11):848-850

2.Change of bacterial community structure during cellulose degradation by the microbial consortium.

Shiqi AI ; Yiquan ZHAO ; Zhiyuan SUN ; Yamei GAO ; Lei YAN ; Hongzhi TANG ; Weidong WANG

Chinese Journal of Biotechnology 2018;34(11):1794-1808

In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth. We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes. Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively. The microbial consortium was mainly composed of 1 phylum, 2 classes, 2 orders, 7 families and 11 genera. With cellulose degradation, bacteria in the consortium showed different growth trends. The relative abundance of Brevibacillus and Caloramator decreased gradually. The relative abundance of Clostridium, Bacillus, Geobacillus and Cohnella increased gradually. The relative abundance of Ureibacillus, Tissierella, Epulopiscium was the highest in peak stage. The relative abundance of Paenibacillus and Ruminococcus did not change obviously in each stage. Above-mentioned 11 main genera all belonged to Firmicutes, which are thermophilic, broad pH adaptable and cellulose or hemicellulose degradable. During cellulose degradation by the microbial consortium, aerobic bacteria were dominant functional bacteria in the initial stage. However, the relative abundance of anaerobic bacteria increased gradually in middle and end stage, and replaced aerobic bacteria to become main bacteria to degrade cellulose.
Bacteria ; classification ; metabolism ; Biodegradation, Environmental ; Cellulose ; metabolism ; DNA, Bacterial ; genetics ; Microbial Consortia ; RNA, Ribosomal, 16S ; genetics

3.First Case Report of Bacteremia Due to Catabacter hongkongensis in a Korean Patient.

Yong Jun CHOI ; Eun Jeong WON ; Soo Hyun KIM ; Myung Geun SHIN ; Jong Hee SHIN ; Soon Pal SUH

Annals of Laboratory Medicine 2017;37(1):84-87

4.Susceptibility of Ceftolozane-Tazobactam and Ceftazidime-Avibactam Against a Collection of β-Lactam-Resistant Gram-Negative Bacteria.

Mark D GONZALEZ ; Allison R MCMULLEN ; Meghan A WALLACE ; Matthew P CROTTY ; David J RITCHIE ; Carey Ann D BURNHAM

Annals of Laboratory Medicine 2017;37(2):174-176

5.Structural insights into glutathione-mediated activation of the master regulator PrfA in Listeria monocytogenes.

Yong WANG ; Han FENG ; Yalan ZHU ; Pu GAO

Protein & Cell 2017;8(4):308-312

6.Markerless DNA deletion based on Red recombination and in vivo I-Sec I endonuclease cleavage in Escherichia coli chromosome.

Meiqin ZHU ; Jian YU ; Changlin ZHOU ; Hongqing FANG

Chinese Journal of Biotechnology 2016;32(1):114-126

Red-based recombineering has been widely used in Escherichia coli genome modification through electroporating PCR fragments into electrocompetent cells to replace target sequences. Some mutations in the PCR fragments may be brought into the homologous regions near the target. To solve this problem in markeless gene deletion we developed a novel method characterized with two-step recombination and a donor plasmid. First, generated by PCR a linear DNA cassette which comprises a I-Sec I site-containing marker gene and homologous arms was electroporated into cells for marker-substitution deletion of the target sequence. Second, after a donor plasmid carrying the I-Sec I site-containing fusion homologous arm was chemically transformed into the marker-containing cells, the fusion arms and the marker was simultaneously cleaved by I-Sec I endonuclease and the marker-free deletion was stimulated by double-strand break-mediated intermolecular recombination. Eleven nonessential regions in E. coli DH1 genome were sequentially deleted by our method, resulting in a 10.59% reduced genome size. These precise deletions were also verified by PCR sequencing and genome resequencing. Though no change in the growth rate on the minimal medium, we found the genome-reduced strains have some alteration in the acid resistance and for the synthesis of lycopene.
Chromosomes, Bacterial ; genetics ; DNA ; Endonucleases ; metabolism ; Escherichia coli ; genetics ; Genetic Engineering ; methods ; Recombination, Genetic ; Sequence Deletion

1.ToxR Is Required for Biofilm Formation and Motility of Vibrio Parahaemolyticus.

2.Change of bacterial community structure during cellulose degradation by the microbial consortium.

3.First Case Report of Bacteremia Due to Catabacter hongkongensis in a Korean Patient.

4.Susceptibility of Ceftolozane-Tazobactam and Ceftazidime-Avibactam Against a Collection of β-Lactam-Resistant Gram-Negative Bacteria.

5.Structural insights into glutathione-mediated activation of the master regulator PrfA in Listeria monocytogenes.

6.Markerless DNA deletion based on Red recombination and in vivo I-Sec I endonuclease cleavage in Escherichia coli chromosome.

7.Identification of Pasteurella canis in a Soft Tissue Infection Caused by a Dog Bite: The First Report in Korea.

8.A Case of Flavobacterium ceti Meningitis.

9.A Novel Integron Gene Cassette Harboring VIM-38 Metallo-β-lactamase in a Clinical Pseudomonas aeruginosa Isolate.

10.First Case Report of Human Infection With Ochrobactrum tritici Causing Bacteremia and Cholecystitis.

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