Objective To investigate the pollution caused by the aerosols and variations of polycyclic aromatic hydrocarbons (PAHs) in a certain submarine cabins,and analyze the pollution source of PAHs,then to evaluate the toxicology of the contaminated submarine air.Methods Andersen cascade sampler was used to collect atmospheric particulate matter samples,during 6 days of continuous submergence of the submarine.The composition and variation of PAHs were analyzed by gas chromatography-mass spectrometry (GC-MS).Research on the main pollution sources of PAHs was made by using principal component analysis.Results Aerosol pollution in various cabins of the submarine was quite serious,furthermore it displayed the feature of pollution by different particle sizes.Particle size distribution analysis revealed that the 8-man cabin,meeting room,command room,torpedo room and engine room were of a unimodal size distribution,and the galley and hot air engine room displayed 3 relatively high peaks.Total PAHs were detected,with the concentration scope from 33.1 ng/m3 to 90.8 ng/m3 and with the 3-ring and 4-ring as the primary components.Total TEQ concentration ranged from 0.077 ng/m3 to 0.251 ng/m3.Conclusions Aerosol pollution in the cabins of the submarine was serious,with the cooking mist and volatile components of fuel as the main sources of PAHs and electrical appliances and laundry detergents as secondary source of pollution.

Objective To investigate the pollution caused by the aerosols and variations of polycyclic aromatic hydrocarbons (PAHs) in a certain submarine cabins,and analyze the pollution source of PAHs,then to evaluate the toxicology of the contaminated submarine air.Methods Andersen cascade sampler was used to collect atmospheric particulate matter samples,during 6 days of continuous submergence of the submarine.The composition and variation of PAHs were analyzed by gas chromatography-mass spectrometry (GC-MS).Research on the main pollution sources of PAHs was made by using principal component analysis.Results Aerosol pollution in various cabins of the submarine was quite serious,furthermore it displayed the feature of pollution by different particle sizes.Particle size distribution analysis revealed that the 8-man cabin,meeting room,command room,torpedo room and engine room were of a unimodal size distribution,and the galley and hot air engine room displayed 3 relatively high peaks.Total PAHs were detected,with the concentration scope from 33.1 ng/m3 to 90.8 ng/m3 and with the 3-ring and 4-ring as the primary components.Total TEQ concentration ranged from 0.077 ng/m3 to 0.251 ng/m3.Conclusions Aerosol pollution in the cabins of the submarine was serious,with the cooking mist and volatile components of fuel as the main sources of PAHs and electrical appliances and laundry detergents as secondary source of pollution.

Objective To investigate and analyze the pollution of aerosol PM 2.5,PM 10 and TSP in the cabins of a certain AIP-submarine during 8 days of continuous submerged operation.Methods The aerosol concentrations of the said substances in the submarine cabins were detected by using TSI8533 aerosol detector.Results In normal submerged operation,the aerosol concentrations in the cabins were all relatively lower,with the galley as an exception,however,they did not exceed the specified national standards.The PM 2.5 concentration in the galley exceeded the allowable limit specified in GB3095-2012.The highest concentrations of PM 2.5,PM 10 and TSP all occurred in the air compressor compartment when the compressor system was under operation.The next highest concentrations of these substances occurred in the main engine room,when damage control operation was under way.TSP concentration was relatively higher in the living cabins of the crew.Conclusions The main sources of aerosol pollution were air compressors and cooking oil fume.The crew and their activities also had some effects on the TSP concentration in the cabins of the submarine.

Objective To investigate and analyze the pollution of aerosol PM 2.5,PM 10 and TSP in the cabins of a certain AIP-submarine during 8 days of continuous submerged operation.Methods The aerosol concentrations of the said substances in the submarine cabins were detected by using TSI8533 aerosol detector.Results In normal submerged operation,the aerosol concentrations in the cabins were all relatively lower,with the galley as an exception,however,they did not exceed the specified national standards.The PM 2.5 concentration in the galley exceeded the allowable limit specified in GB3095-2012.The highest concentrations of PM 2.5,PM 10 and TSP all occurred in the air compressor compartment when the compressor system was under operation.The next highest concentrations of these substances occurred in the main engine room,when damage control operation was under way.TSP concentration was relatively higher in the living cabins of the crew.Conclusions The main sources of aerosol pollution were air compressors and cooking oil fume.The crew and their activities also had some effects on the TSP concentration in the cabins of the submarine.

Objective:[WT5BZ] The effects of exogenous vitamin C (VC) and glutathione (GSH) on ultraweak spontaneous luminescence of culturing pulmonary alveolus macrophages from rabbits were studiesd. [WT5FZ]Methods:[WT5BZ] In a special thermostat, which was passed through by airs with various concentrations of oxygen, the alveolar macrophages (AMs) were cultured in DMEM medium with VC or GSH, and the spontaneous luminescence of culturing AMs was examined by a luminometer. [WT5FZ]Results:[WT5BZ] when VC in medium was over 0.3 mmol/L, it could significantly enhance the oxidative luminescence of cells exposed to O 2 and cell death was resulted. However, when the cells were exposed to air without O 2 there was no significant effect. On the contrary,the lower concentration of VC (0.03 mmol/L) as well as GSH could reduce the spontaneous luminescence of cells exposed to a high concentration (99.1%) of O 2 in air. [WT5FZ]Conclusion:[WT5BZ] The results show that the spontaneous oxidation of culturing cells is an important reason for the ultraweak luminescence. High concentration of VC can promote cellular oxidative damage in vitro, but the exogenous GSH has a protective effect against oxidization in culturing cells.