Objective To investigate the effectiveness of self-prescribed Changning Formula combined with Mesalazine in the treatment of ulcerative colitis(UC),and to analyze its effect on protein kinase R-like endoplasmic reticulum kinase(PERK)/nuclear factor κB(NF-κB)pathway.Methods A total of 100 patients with UC admitted to our hospital from January 2021 to January 2024 were selected and divided into control group and combined group by random number table method,with 50 cases in each group.The control group was given Mesalazine,and the combined group was given self-prescribed Changning Formula combined with Mesalazine.After 2 months of treatment,the effectivenesses in two groups were compared.The clinical symp-toms,disease activity(Mayo index score),colonoscopy pathological status(Baron score),mucosal healing status(Geboes index)and PERK/NF-κB pathway-related protein levels in peripheral blood mononuclear cells[phosphorylated PERK(P-PERK),phos-phorylated eukaryotic initiation factor 2α(P-eIF2α),phosphorylated NF-κB(P-NF-κB)],the levels of PERK/NF-κB pathway downstream factors[interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-8(IL-8)]before and after treatment,as well as adverse reactions were compared between the two groups.Results The total effective rate of the combined group was 96.00％,which was higher than that of the control group(76.00％),and the clinical symptom score was lower than that of the control group(both P＜0.05).After treatment,the Mayo index score,Baron score,and Geboes index score in the combined group were(2.32±0.28),(0.51±0.11),and(0.41±0.10),respectively,which were lower than those in the control group[(3.45±0.50),(1.17±0.28),and(1.00±0.22),all P＜0.05].After treatment,the levels of P-PERK,P-eIF2α,and P-NF-κB in peripheral blood mononuclear cells in the combined group were(1.07±0.14),(0.88±0.14),and(0.95±0.18),respectively,which were lower than those in the control group[1.85±0.20),(1.29±0.23),and(1.14±0.23),all P＜0.05].The levels of serum IL-1 β,TNF-α,IL-6,and IL-8 in the combined group after treatment were(4.02±1.26)pg/mL,(6.15±1.64)pg/mL,(8.52±2.04)pg/mL,and(25.34±5.28)pg/mL,respectively,which were lower than those in the control group[(6.00±1.54)pg/mL,(8.79±2.33)pg/mL,(11.23±3.19)pg/mL,and(41.78±9.34)pg/mL,all P＜0.05].The inci-dence of adverse reactions in the combined group was 4.00％,and the difference was not significant compared to the control group(8.00％,P＞0.05).Conclusion The effectiveness and safety of self-prescribed Changning Formula combined with Me-salazine in the treatment of UC are confirmed.It can improve clinical symptoms,control disease progression and alleviate inflam-matory response,the mechanism of which may be related to inhibition PERK/NF-κB pathway activation.

Objective To investigate the effectiveness of self-prescribed Changning Formula combined with Mesalazine in the treatment of ulcerative colitis(UC),and to analyze its effect on protein kinase R-like endoplasmic reticulum kinase(PERK)/nuclear factor κB(NF-κB)pathway.Methods A total of 100 patients with UC admitted to our hospital from January 2021 to January 2024 were selected and divided into control group and combined group by random number table method,with 50 cases in each group.The control group was given Mesalazine,and the combined group was given self-prescribed Changning Formula combined with Mesalazine.After 2 months of treatment,the effectivenesses in two groups were compared.The clinical symp-toms,disease activity(Mayo index score),colonoscopy pathological status(Baron score),mucosal healing status(Geboes index)and PERK/NF-κB pathway-related protein levels in peripheral blood mononuclear cells[phosphorylated PERK(P-PERK),phos-phorylated eukaryotic initiation factor 2α(P-eIF2α),phosphorylated NF-κB(P-NF-κB)],the levels of PERK/NF-κB pathway downstream factors[interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-8(IL-8)]before and after treatment,as well as adverse reactions were compared between the two groups.Results The total effective rate of the combined group was 96.00％,which was higher than that of the control group(76.00％),and the clinical symptom score was lower than that of the control group(both P＜0.05).After treatment,the Mayo index score,Baron score,and Geboes index score in the combined group were(2.32±0.28),(0.51±0.11),and(0.41±0.10),respectively,which were lower than those in the control group[(3.45±0.50),(1.17±0.28),and(1.00±0.22),all P＜0.05].After treatment,the levels of P-PERK,P-eIF2α,and P-NF-κB in peripheral blood mononuclear cells in the combined group were(1.07±0.14),(0.88±0.14),and(0.95±0.18),respectively,which were lower than those in the control group[1.85±0.20),(1.29±0.23),and(1.14±0.23),all P＜0.05].The levels of serum IL-1 β,TNF-α,IL-6,and IL-8 in the combined group after treatment were(4.02±1.26)pg/mL,(6.15±1.64)pg/mL,(8.52±2.04)pg/mL,and(25.34±5.28)pg/mL,respectively,which were lower than those in the control group[(6.00±1.54)pg/mL,(8.79±2.33)pg/mL,(11.23±3.19)pg/mL,and(41.78±9.34)pg/mL,all P＜0.05].The inci-dence of adverse reactions in the combined group was 4.00％,and the difference was not significant compared to the control group(8.00％,P＞0.05).Conclusion The effectiveness and safety of self-prescribed Changning Formula combined with Me-salazine in the treatment of UC are confirmed.It can improve clinical symptoms,control disease progression and alleviate inflam-matory response,the mechanism of which may be related to inhibition PERK/NF-κB pathway activation.

Objective     To identify the risk factors for postoperative recurrence of peripheral solid small-nodule lung cancer (PSSNLC) (T≤2 cm), and to explore the effects of surgery types on prognosis. Methods     We extracted data from Western China Lung Cancer Database (WCLCD), a prospectively maintained database at the Department of Thoracic Surgery, West China Hospital, Sichuan University, and Surveillance, Epidemiology, and End Results (SEER)  database for peripheral solid small-nodule lung cancer patients (T≤2 cm N0M0, stageⅠ) who underwent surgery between 2005 and 2016. We used univariable and multivariable logistic regression to analyze risk factors for recurrence of PSSNLC. We applied propensity-score matching to compare the long-term results of segmentectomy and lobectomy, as well as the survival of patients from WCLCD and SEER. We finally included 4 800 patients with PSSNLC (T≤2 cm N0M0)(WCLCD: SEER=354∶4 446). We matched 103 segmentectomies and 350 lobectomies in T≤1 cm, and 280 segmentectomies and 1 067 lobectomies in 1 cm

Objective To understand the clinical distribution characteristics and antimicrobial resistance of pathogens causing healthcare-associated infection(HAI) in a comprehensive hospital.Methods Clinical data of patients with HAI in this hospital between May 2012 and May 2015 were collected,the distribution and antimicrobial resistance of pathogens isolated from patients were analyzed.Results A total of 6 563 cases of HAI occurred among 183 850 patients,incidence of HAI was 3.57％,445 patients were isolated at least two kinds of pathogens,375 (84.27％) patients were isolated two kinds of pathogens,132 of whom were infected with both gram negative bacilli.4 478 specimens were sent for pathogenic detection,2 503 (55.90％) of which were isolated pathogens;a total of 2 755 pathogens were isolated,including 1 713(62.18％) strains of gram-negative bacilli,732(26.57％) gram positive cocci,304(11.03％) yeast-like fungi,and 6(0.22％) anaerobic bacteria.524(19.02％)strains were mainly from patients in department of neurology.The main specimen was sputum (n =1 340,48.64％).The isolation rates of carbapenem-resistant Escherichia coli (CREC),Klebsiella pneumoniae (CRKP),Acinetobacter baumnannii (CRAB),and Pseudomonas aeruginosa (CRPA) were 0.39％ (2/510),1.66％ (3/181),59.14％ (207/ 350),and 5.29 ％ (11/208) respectively;isolation rate of methicillin-resistant Staphylococcus aureus (MRSA) was 21.55％(25/116).Conclusion Multidrug-resistant organisms causing HAl are various,it is necessary to understand distribution characteristics and prevalence of pathogens,monitor multidrug-resistant organisms,and implement contact isolation measures,so as to prevent the outbreak of HAI.

Objective To compare the yield and speed of detection of Salmonella subsp, enterica serotypo Paratyphi A from the blood of patients with suspected paratyphoid fever A. Methods With the BacT/ALERT 3D system and paired aerobic and anaerobic bottles (AEB, ANB) that were each filled with 5 ml of blood, the blood culture of 13 500 suspected paratyphoid fever A patients were performed. Results A total of 4 060 isolates were detected. About 3 149 were detected from both AEB and ANB. Four hundred and sixty-one isolates were detected only from the AEB and 450 were only from the ANB. The detection rates of the AEB and ANB were all 26.7% (χ<'2>=0.023, P=0.880). The increased detection rate attributed to the additional blood volume in the AEB and ANB were 11.3% and 11.1%, respectively. The detection speed differed between the two medium formulations. The time to detection was (23.66±15.89) h and (25.48±16.92) h for3 149 isolates, respectively (t=7.007, P<0.01).The mean time to detection was 31.80±20. 97 for 461 isolates discovered with AEB and (33.45±20.72) h for 450 isolates discovered with ANB. Conclusion The blood volume is an important factor in determining the detection rate of blood culture. Although no statistical difference for positive rate was found between the AEB and ANB, more isolates was detected earlier in AEB.

Objective To understand the elonal expansion and genetic diversity of Salmonella en-terica semtype Paratyphi A (SPA) and to construct a typing method to determine the epidemic clones of the isolates. Methods Antimicrobial susceptibility testing was performed with 3980 SPA isolates by the cen-trolled Kirby-Bauer disc diffusion technique on Muller-Hinton agar plates. A total of 15 SPA with nalidixie acid resistance for mutations in gyrA, gyrB, gyrC and gyrE genes within the quinolone-resistant determina-tion region (QRDR) were examined. Subtyping of 121 isolates of SPA from seven counties in Yuxi were studied using pulsed-field gel eleetrophoresis (PFGE) analysis following digestion of chromosomal DNA with restriction endanucleases Spe Ⅰ and Xba Ⅰ. PFGE patterns were analyzed by duster analysis. Results The nalidixic acid-susceptible isolates predominated in 1999 but was replaced by nalidixic acid -resistant (NAR) isolates after 2000. Amplification by PCR and sequencing of the genes with subsets of 15 NAR strains re-vealed that the resistance mechanisms had resulted from single point mutations in the gyrA gene. Spe Ⅰ and Xba Ⅰ digestion of 121 isolates gave five and four different PFGE patterns with the predominance of the Spe Ⅰ 01 and Spe Ⅰ 02 (or the Xba Ⅰ 01) epidemic patterns, respectively. Spe Ⅰ 01 and Spe Ⅰ 02 consisted of 37.2% and 57.9% of isolates, respectively, or Xba Ⅰ 01 consisted of 95.0% of isolates. Conclusion The incidence of resistance to nalidixic acid of the isolates increased during the study period. PFGE patterns Spe Ⅰ 01 and Spe Ⅰ 02 (or Xba Ⅰ 01), the main clones of the epidemics, are highly prevalent in Yuxi. PFGE with Spe Ⅰ and Xba Ⅰ is a useful technique to differentiate SPA.