Objective To investigate the effect of ratanasampil (RNSP) which is traditional Chinese Tibet-Medicines on the β-amyloid peptide as a therapeutic target in Alzheimer's disease.Methods Thirteen female mice of 13-14 months old with Tg2576 transgene and ten BL6 × SJL mice as control were used in drug test All mice sacrificed after 8 weeks of RNSP treatment at 15-16 months of age.Open field activity and Y-maze tests were performed for animal behavioral change and memory ability.The β-amyloid peptides (Aβ1-40 and Aβ1-42) and β-amyloid precursor protein (APP) in Tg2576 mice brain were measured with western blot and enzyme linked immunosorbent assay (ELISA).Immunostaining with 1 E8 ( 1: 25 ) was carried out on brain sections of RNSP and vehicle-treated mice.Results The training times of Y-maze test decreased in RNSP-treated Tg2576 mice (P ＜0.01 ).After 2 months of RNSP treatment, a decrease in open field behavior was seen in Tg2576 mice ( P ＜ 0.05 ).The level of Aβ1-40 and Aβ1-42 in the brain was significantly decreased after RNSP treatment ( P ＜ 0.05 ).The Aβ42/Aβ40 ratio was significantly decreased after RNSP treatment as compared to vehicle-treated mice ( P ＜ 0.05 ) .But levels of APP in brain unchanged.Preliminary plaque counting of the sections showed reduced plaque numbers in the drug-treated mice in brain.Conclusions These results suggest that ratanasampil may reduce β-amyloid peptide production in brain to improve the learning and memory ability of Tg2576 mice of Alzheimer's disease.

Objective To investigate the effect of ratanasampil (RNSP) which is traditional Chinese Tibet-Medicines on the β-amyloid peptide as a therapeutic target in Alzheimer's disease.Methods Thirteen female mice of 13-14 months old with Tg2576 transgene and ten BL6 × SJL mice as control were used in drug test All mice sacrificed after 8 weeks of RNSP treatment at 15-16 months of age.Open field activity and Y-maze tests were performed for animal behavioral change and memory ability.The β-amyloid peptides (Aβ1-40 and Aβ1-42) and β-amyloid precursor protein (APP) in Tg2576 mice brain were measured with western blot and enzyme linked immunosorbent assay (ELISA).Immunostaining with 1 E8 ( 1: 25 ) was carried out on brain sections of RNSP and vehicle-treated mice.Results The training times of Y-maze test decreased in RNSP-treated Tg2576 mice (P ＜0.01 ).After 2 months of RNSP treatment, a decrease in open field behavior was seen in Tg2576 mice ( P ＜ 0.05 ).The level of Aβ1-40 and Aβ1-42 in the brain was significantly decreased after RNSP treatment ( P ＜ 0.05 ).The Aβ42/Aβ40 ratio was significantly decreased after RNSP treatment as compared to vehicle-treated mice ( P ＜ 0.05 ) .But levels of APP in brain unchanged.Preliminary plaque counting of the sections showed reduced plaque numbers in the drug-treated mice in brain.Conclusions These results suggest that ratanasampil may reduce β-amyloid peptide production in brain to improve the learning and memory ability of Tg2576 mice of Alzheimer's disease.