Objective:To explore the real experience of patients with diffuse large B-cell lymphoma in clinical trials of chimeric antigen receptor T (CAR-T) cell therapy, providing reference for clinical practice.Methods:This study was descriptive qualitative research. From January to December 2024, 15 patients with diffuse large B-cell lymphoma who completed a clinical trial of CAR-T cell therapy in Ward of Biological Immunotherapy of the First Affiliated Hospital of Zhengzhou University were selected by purposive sampling method for semi-structured interviews. The data was analyzed by content analysis method.Results:The experience of patients with diffuse large B-cell lymphoma participating in the clinical trial of CAR-T cell therapy were summarized into three themes and 12 sub-themes, namely, the pre-enrollment situation and attitude towards the clinical trial: survival from adversity (caught in the dilemma of treatment, grasping the last hope, survival desire overcoming fear, active psychological construction), social support and power source in the clinical trial: support network (unnoticeable personal strength, multi-dimensional family support system, professional medical support system, weak peer support system), and experience and feeling after CAR-T cell therapy: positive experience driven by efficacy (satisfaction with treatment effectiveness and clinical trials, pleased with reduced adverse reactions, increased confidence in treating diseases, adverse reactions were not scary) .Conclusions:Patients with diffuse large B-cell lymphoma have an overall positive experience during clinical trials of CAR-T cell therapy, but the negatives should not be ignored. Healthcare professionals should strengthen the health education of clinical trials, assist patients to improve the social support network, make up for the short board of patient support, alleviate the adverse reactions of patients, and then increase the enthusiasm of patients to participate in clinical trials.

Autoimmunity and the activation of immune cells are closely related to the occurrence of chronic spontaneous urticaria, while the gut microbiota participates in multiple physiological activities including the regulation of the host's immunity. Gut microbiota in patients with chronic spontaneous urticaria has unique structural composition and metabolic features. Gut microbiota imbalance and secondary short-chain fatty acid deficiency may be important causes for the occurrence of the disease and aggravation of inflammation. Studies have shown that probiotics can improve the clinical manifestations of patients with urticaria. This review summarizes the research progress in the correlations of gut microbiota and short-chain fatty acids with chronic spontaneous urticaria.

OBJECTIVE To investigate the pharmacodynamics and related mechanisms of Anmei Dan on hippocampus of mice with coronary heart disease complicated with depression.METHODS The coronary heart disease model combined with chronic and unpredictable mild stress depression model was established,and the mice were randomly divided into blank group,model group,low dose group(1.5 g·kg-1),high dose group(3 g·kg-1)and atorvastatin group(0.3 g·kg-1).Sucrose preference test,open field test and forced swimming test were used to evaluate the behavioral changes of mice.qPCR and ELISA were employed to ascertain the mR-NA expression of interleukin-1β(IL-1β),interleukin-6(IL-6)and tumour necrosis factor-α(TNF-α)in the hippocampus.The changes of neurons and Nissl bodies in CA1,CA3 and DG regions of hippocampus were observed by Nissl staining.The expression of key proteins was detected by Western blot.RESULTS Compared with the blank group,the sucrose preference rate of mice in the model group was decreased(P<0.01),the forced swimming immobility time was extended(P<0.01)and the movement distance in the open field experiment was not significantly changed.The levels of total cholesterol(TC),triglyceride(TG)and low density lipopro-tein cholesterol(LDL-C)were increased significantly(P<0.01)and the level of high density lipoprotein cholesterol(HDL-C)was decreased significantly(P<0.01).The mRNA level and content of IL-1β,IL-6 and TNF-α were significantly increased(P<0.01).The expression of glutamate receptor 1(GluR1),postsynaptic densitin-95(PSD-95),brain-derived neurotrophic factor(BDNF)and phosphorylated calmodulin-dependent kinase(p-CaMKⅡ)in hippocampus was decreased(P<0.01).The expression of cytoskeletal activity regulatory protein(Arc)was increased(P<0.01).In the model group,the cell structure was irregular and differ-ent degrees of damage occurred,the Nissl bodies decreased or disappeared,and the cell membrane broke.Compared with the model group,the sucrose preference rate of mice in each administration group was significantly increased(P<0.01),the immobility time in forced swimming experiment was significantly decreased(P<0.01),and the levels of TC,TG and LDL-C of mice in Anmei Dan groups and atorvastatin group were decreased(P<0.01),while the level of HDL-C increased(P<0.01).The mRNA levels and con-tent of IL-1β,IL-6 and TNF-α in Anmei Dan groups and atorvastatin group were decreased(P<0.01).The expression of GluR1,PSD-95,BDNF and p-CaMKⅡ in hippocampal tissue of Anmei Dan groups were increased(P<0.01),and the expression of Arc was decreased(P<0.01).The morphology and structure of the cells in the Anmei Dan group and the atorvastatin group were improved,with varying degrees of increased Nissl bodies and relatively intact cell membranes.CONCLUSION Anmei Dan can effectively im-prove blood lipids and depression-like behavior of coronary heart disease mice complicated with depression.It can inhibit pro-inflam-matory factors,increase the expression of neurotrophic factors,effectively improve synaptic related proteins,and reduce the damage to neurons,thus effectively preventing the exacerbation of coronary heart disease and depression comorbidity.

Autoimmunity and the activation of immune cells are closely related to the occurrence of chronic spontaneous urticaria, while the gut microbiota participates in multiple physiological activities including the regulation of the host's immunity. Gut microbiota in patients with chronic spontaneous urticaria has unique structural composition and metabolic features. Gut microbiota imbalance and secondary short-chain fatty acid deficiency may be important causes for the occurrence of the disease and aggravation of inflammation. Studies have shown that probiotics can improve the clinical manifestations of patients with urticaria. This review summarizes the research progress in the correlations of gut microbiota and short-chain fatty acids with chronic spontaneous urticaria.

OBJECTIVE To investigate the pharmacodynamics and related mechanisms of Anmei Dan on hippocampus of mice with coronary heart disease complicated with depression.METHODS The coronary heart disease model combined with chronic and unpredictable mild stress depression model was established,and the mice were randomly divided into blank group,model group,low dose group(1.5 g·kg-1),high dose group(3 g·kg-1)and atorvastatin group(0.3 g·kg-1).Sucrose preference test,open field test and forced swimming test were used to evaluate the behavioral changes of mice.qPCR and ELISA were employed to ascertain the mR-NA expression of interleukin-1β(IL-1β),interleukin-6(IL-6)and tumour necrosis factor-α(TNF-α)in the hippocampus.The changes of neurons and Nissl bodies in CA1,CA3 and DG regions of hippocampus were observed by Nissl staining.The expression of key proteins was detected by Western blot.RESULTS Compared with the blank group,the sucrose preference rate of mice in the model group was decreased(P<0.01),the forced swimming immobility time was extended(P<0.01)and the movement distance in the open field experiment was not significantly changed.The levels of total cholesterol(TC),triglyceride(TG)and low density lipopro-tein cholesterol(LDL-C)were increased significantly(P<0.01)and the level of high density lipoprotein cholesterol(HDL-C)was decreased significantly(P<0.01).The mRNA level and content of IL-1β,IL-6 and TNF-α were significantly increased(P<0.01).The expression of glutamate receptor 1(GluR1),postsynaptic densitin-95(PSD-95),brain-derived neurotrophic factor(BDNF)and phosphorylated calmodulin-dependent kinase(p-CaMKⅡ)in hippocampus was decreased(P<0.01).The expression of cytoskeletal activity regulatory protein(Arc)was increased(P<0.01).In the model group,the cell structure was irregular and differ-ent degrees of damage occurred,the Nissl bodies decreased or disappeared,and the cell membrane broke.Compared with the model group,the sucrose preference rate of mice in each administration group was significantly increased(P<0.01),the immobility time in forced swimming experiment was significantly decreased(P<0.01),and the levels of TC,TG and LDL-C of mice in Anmei Dan groups and atorvastatin group were decreased(P<0.01),while the level of HDL-C increased(P<0.01).The mRNA levels and con-tent of IL-1β,IL-6 and TNF-α in Anmei Dan groups and atorvastatin group were decreased(P<0.01).The expression of GluR1,PSD-95,BDNF and p-CaMKⅡ in hippocampal tissue of Anmei Dan groups were increased(P<0.01),and the expression of Arc was decreased(P<0.01).The morphology and structure of the cells in the Anmei Dan group and the atorvastatin group were improved,with varying degrees of increased Nissl bodies and relatively intact cell membranes.CONCLUSION Anmei Dan can effectively im-prove blood lipids and depression-like behavior of coronary heart disease mice complicated with depression.It can inhibit pro-inflam-matory factors,increase the expression of neurotrophic factors,effectively improve synaptic related proteins,and reduce the damage to neurons,thus effectively preventing the exacerbation of coronary heart disease and depression comorbidity.

Objective:To explore the real experience of patients with diffuse large B-cell lymphoma in clinical trials of chimeric antigen receptor T (CAR-T) cell therapy, providing reference for clinical practice.Methods:This study was descriptive qualitative research. From January to December 2024, 15 patients with diffuse large B-cell lymphoma who completed a clinical trial of CAR-T cell therapy in Ward of Biological Immunotherapy of the First Affiliated Hospital of Zhengzhou University were selected by purposive sampling method for semi-structured interviews. The data was analyzed by content analysis method.Results:The experience of patients with diffuse large B-cell lymphoma participating in the clinical trial of CAR-T cell therapy were summarized into three themes and 12 sub-themes, namely, the pre-enrollment situation and attitude towards the clinical trial: survival from adversity (caught in the dilemma of treatment, grasping the last hope, survival desire overcoming fear, active psychological construction), social support and power source in the clinical trial: support network (unnoticeable personal strength, multi-dimensional family support system, professional medical support system, weak peer support system), and experience and feeling after CAR-T cell therapy: positive experience driven by efficacy (satisfaction with treatment effectiveness and clinical trials, pleased with reduced adverse reactions, increased confidence in treating diseases, adverse reactions were not scary) .Conclusions:Patients with diffuse large B-cell lymphoma have an overall positive experience during clinical trials of CAR-T cell therapy, but the negatives should not be ignored. Healthcare professionals should strengthen the health education of clinical trials, assist patients to improve the social support network, make up for the short board of patient support, alleviate the adverse reactions of patients, and then increase the enthusiasm of patients to participate in clinical trials.

Objective:To explore anxiety and impulsivity in adult female patients with anorexia nervosa (AN), and the relationship with different dimensions of the core clinical symptoms.Methods:From June 2014 to October 2022, 96 patients with AN, from both outpatient clinics and inpatient wards, were enrolled from Peking University Sixth Hospital. Additionally, 51 control participants were recruited from the community. State-trait Anxiety Inventory (STAI) and Barratt Impulsiveness Scale (BIS-11) were used to evaluate the anxiety and impulsivity of each group.The Eating Disorder Inventory-1 (EDI-1) was used to assess clinical and other psychological characteristics of ED.Correlation and regression analysis were employed to explore the relationship between anxiety, impulsivity, and core clinical symptoms of eating disorders.Results:(1) The total EDI-1 score for the AN group ((233.7±48.4) vs. (184.2±30.4), t=7.58, P<0.001) and scores in 7 subscales (Drive for Thinness: t=5.25, P<0.001; Bulimia: t=6.05, P<0.001; Ineffectiveness: t=7.91, P<0.001; Perfectionism: t=3.05, P=0.003; Interpersonal Distrust: t=5.50, P<0.001; Interoceptive Awareness: t=7.00, P<0.001; Maturity Fears: t=2.46, P=0.015) were significantly higher than the control group. (2) The AN group had significantly higher levels of state anxiety ( t=8.60, P<0.001), trait anxiety( t=7.40, P<0.001), total impulsivity( t=3.55, P<0.001), attentional impulsiveness( t=2.43, P=0.017) and motor impulsiveness( t=4.29, P<0.001) compared to the control group.(3)Correlation analysis showed that state anxiety, trait anxiety, attentional impulsiveness, motor impulsiveness, and non-planning impulsiveness were positively correlated with the drive for thinness( r=0.522, 0.577, 0.272, 0.387, 0.209, all P<0.05); State anxiety, trait anxiety, attentional impulsiveness, motor impulsiveness, and non-planning impulsiveness were also positively correlated with bulimia ( r=0.402, 0.471, 0.304, 0.514, 0.466, all P<0.01); Attentional impulsiveness, state anxiety, and trait anxiety were positively correlated with body dissatisfaction ( r=0.333, 0.448, 0.409, all P<0.01). Further ridge regression analysis indicated that trait anxiety in AN patients was an influencing factor of the drive for thinness ( t=3.13, P=0.002), state anxiety was an influencing factor of body dissatisfaction ( t=1.99, P=0.050), and motor impulsiveness and non-planning impulsiveness were the influencing factors of bulimia ( t=2.92, 2.79, all P<005). Conclusions:Patients with anorexia nervosa have higher anxiety and impulsivity, and the levels of anxiety and impulsivity in different dimensions are related to the core clinical symptoms of AN patients.

Objective:To explore anxiety and impulsivity in adult female patients with anorexia nervosa (AN), and the relationship with different dimensions of the core clinical symptoms.Methods:From June 2014 to October 2022, 96 patients with AN, from both outpatient clinics and inpatient wards, were enrolled from Peking University Sixth Hospital. Additionally, 51 control participants were recruited from the community. State-trait Anxiety Inventory (STAI) and Barratt Impulsiveness Scale (BIS-11) were used to evaluate the anxiety and impulsivity of each group.The Eating Disorder Inventory-1 (EDI-1) was used to assess clinical and other psychological characteristics of ED.Correlation and regression analysis were employed to explore the relationship between anxiety, impulsivity, and core clinical symptoms of eating disorders.Results:(1) The total EDI-1 score for the AN group ((233.7±48.4) vs. (184.2±30.4), t=7.58, P<0.001) and scores in 7 subscales (Drive for Thinness: t=5.25, P<0.001; Bulimia: t=6.05, P<0.001; Ineffectiveness: t=7.91, P<0.001; Perfectionism: t=3.05, P=0.003; Interpersonal Distrust: t=5.50, P<0.001; Interoceptive Awareness: t=7.00, P<0.001; Maturity Fears: t=2.46, P=0.015) were significantly higher than the control group. (2) The AN group had significantly higher levels of state anxiety ( t=8.60, P<0.001), trait anxiety( t=7.40, P<0.001), total impulsivity( t=3.55, P<0.001), attentional impulsiveness( t=2.43, P=0.017) and motor impulsiveness( t=4.29, P<0.001) compared to the control group.(3)Correlation analysis showed that state anxiety, trait anxiety, attentional impulsiveness, motor impulsiveness, and non-planning impulsiveness were positively correlated with the drive for thinness( r=0.522, 0.577, 0.272, 0.387, 0.209, all P<0.05); State anxiety, trait anxiety, attentional impulsiveness, motor impulsiveness, and non-planning impulsiveness were also positively correlated with bulimia ( r=0.402, 0.471, 0.304, 0.514, 0.466, all P<0.01); Attentional impulsiveness, state anxiety, and trait anxiety were positively correlated with body dissatisfaction ( r=0.333, 0.448, 0.409, all P<0.01). Further ridge regression analysis indicated that trait anxiety in AN patients was an influencing factor of the drive for thinness ( t=3.13, P=0.002), state anxiety was an influencing factor of body dissatisfaction ( t=1.99, P=0.050), and motor impulsiveness and non-planning impulsiveness were the influencing factors of bulimia ( t=2.92, 2.79, all P<005). Conclusions:Patients with anorexia nervosa have higher anxiety and impulsivity, and the levels of anxiety and impulsivity in different dimensions are related to the core clinical symptoms of AN patients.

Objective To explore the functional connectivity (FC) of the fronto-striatal circuitry in patients with bulimia nervosa (BN) based on the resting-state fMRI and its correlation with the inhibitory function.Methods 27 medication-naive female patients with BN and 27 age-and education-matched female healthy control subjects were included in the study.All the subjects performed a stop signal task (SST) and underwent the resting-state fMRI scan,separately.The FC between striatal subregions and the frontal cortex was analyzed.Results Compared with healthy controls,FC between the right ventral rostral putamen (VRP) and the right supplementary motor areas (SMA) decreased (MNI coordinate:x =3,y =-15,z =51,K =27) in patients with BN.And the FC was also decreased between the right VRP and premotor area(PM) (MNI coordinate:x =27,y =0,z =57,K =44).FC between bilateral dorsal caudal putamen (DCP) (MNI coordinate:x=21,y=-6,z=48,K=43) and the right PM(MNI coordinate:x=21,y=-12,z=57,K=24) was decreased in patients with BN (P＜0.05,Alphasim corrected,voxel P＜0.005,clusters ≥ 20 voxels).FC between the right VRP and right SMA was negatively correlated with the stop signal reaction time (SSRT) in patients with BN (r=-0.595,P=0.004).The FC between right DCP and right PM was positively correlated with the impulsivity regulation subscale scores of the Eating Disorder Inventory-Ⅱ in patients with BN(r=0.483,P=0.023).Conclusion There is disrupted FC between the striatum and motor cortex in medication-naive female patients with BN based on resting-state fMRI,which may be related to impaired inhibitory control in patients with BN.