Objective To observe the value of MRI quantified uterine indictors in late pregnancy for predicting uterine inertia in delivery.Methods A total of 182 pregnant women were retrospectively collected and were divided into uterine inertia group(inertia group,n=67)and non-uterine inertia group(non-inertia group,n=115)based on delivery or cesarean section records.MRI quantified uterine indicators were compared between groups,and those being statistically different were included to build a multivariate logistic regression model for predicting uterine inertia in delivery.The predictive performance of this model was evaluated using receiver operating characteristic(ROC)curve and the area under the curve(AUC).Results The inertia group had more previous uterine surgeries,also higher proportion of in vitro fertilization and embryo transfer(IVF-ET)and cesarean sections compared to the non-inertia group(all P＜0.05).In late pregnancy,the longitudinal diameter of the uterus,cervical length and the thickness of the myometrium at the placental attachment site in inertia group were larger than those in non-inertia group(all P＜0.05).Increased times of previous uterine surgeries,undergoing IVF-ET,as well as increased longitudinal diameter of the uterus and cervical length in late pregnancy were all independent predictors of uterine inertia in delivery(all P＜0.05).AUC of the multivariate logistic regression model established based on the above factors for predicting uterine inertia in delivery was 0.733.Conclusion MRI quantified uterine indictors in late pregnancy could be used to predict uterine inertia in delivery.

Objective To observe the value of MRI quantified uterine indictors in late pregnancy for predicting uterine inertia in delivery.Methods A total of 182 pregnant women were retrospectively collected and were divided into uterine inertia group(inertia group,n=67)and non-uterine inertia group(non-inertia group,n=115)based on delivery or cesarean section records.MRI quantified uterine indicators were compared between groups,and those being statistically different were included to build a multivariate logistic regression model for predicting uterine inertia in delivery.The predictive performance of this model was evaluated using receiver operating characteristic(ROC)curve and the area under the curve(AUC).Results The inertia group had more previous uterine surgeries,also higher proportion of in vitro fertilization and embryo transfer(IVF-ET)and cesarean sections compared to the non-inertia group(all P＜0.05).In late pregnancy,the longitudinal diameter of the uterus,cervical length and the thickness of the myometrium at the placental attachment site in inertia group were larger than those in non-inertia group(all P＜0.05).Increased times of previous uterine surgeries,undergoing IVF-ET,as well as increased longitudinal diameter of the uterus and cervical length in late pregnancy were all independent predictors of uterine inertia in delivery(all P＜0.05).AUC of the multivariate logistic regression model established based on the above factors for predicting uterine inertia in delivery was 0.733.Conclusion MRI quantified uterine indictors in late pregnancy could be used to predict uterine inertia in delivery.

Objective To observe the value of placental MRI morphological indicators for diagnosing placenta accreta spectrum(PAS).Methods Totally 214 pregnant women who underwent prenatal placental MR examination were retrospectively enrolled and divided into PAS group(n=110)and non PAS group(n=104)based on the presence of PAS or not,also into PAS subgroup and non PAS subgroup during different weeks of pregnancy.Clinical and MRI data were compared between groups and subgroups,and the value of placental MRI morphological indicators for diagnosing PAS was analyzed.Results Significant differences of pregnant women's age,gestational week at MR examination,delivery gestational week,times of pregnancies,deliveries,cesarean sections,miscarriages and uterine clearances,as well as proportion of placenta praevia were found between groups(all P＜0.05).During 28-32 weeks and 32+1-36 weeks of pregnancy,significant differences of placental diameter,area and volume were found between subgroups(all P＜0.05),while no significant differences of placental MRI morphological indicators at gestational age＞36 weeks was found between subgroups(all P＞0.05).The area under the curve(AUC)of placental diameter,area and volume for diagnosing PAS at 28-32 weeks of pregnancy was 0.749,0.729 and 0.791,respectively,at 32+1-36 weeks was 0.718,0.686 and 0.614,respectively,being not significantly different(all P＞0.05).Conclusion Placental MRI morphological indicators at 28-36 weeks of pregnancy were helpful for diagnosing PAS.

Objective To observe the value of placental MRI morphological indicators for diagnosing placenta accreta spectrum(PAS).Methods Totally 214 pregnant women who underwent prenatal placental MR examination were retrospectively enrolled and divided into PAS group(n=110)and non PAS group(n=104)based on the presence of PAS or not,also into PAS subgroup and non PAS subgroup during different weeks of pregnancy.Clinical and MRI data were compared between groups and subgroups,and the value of placental MRI morphological indicators for diagnosing PAS was analyzed.Results Significant differences of pregnant women's age,gestational week at MR examination,delivery gestational week,times of pregnancies,deliveries,cesarean sections,miscarriages and uterine clearances,as well as proportion of placenta praevia were found between groups(all P＜0.05).During 28-32 weeks and 32+1-36 weeks of pregnancy,significant differences of placental diameter,area and volume were found between subgroups(all P＜0.05),while no significant differences of placental MRI morphological indicators at gestational age＞36 weeks was found between subgroups(all P＞0.05).The area under the curve(AUC)of placental diameter,area and volume for diagnosing PAS at 28-32 weeks of pregnancy was 0.749,0.729 and 0.791,respectively,at 32+1-36 weeks was 0.718,0.686 and 0.614,respectively,being not significantly different(all P＞0.05).Conclusion Placental MRI morphological indicators at 28-36 weeks of pregnancy were helpful for diagnosing PAS.

Aim To explore the toxicity and safety of five kinds of known positive drugs, cyclophosphamide, acetyl salicylic acid, tetracycline hydrochloride, dexa-methasone acetate and azacitidine, using zebrafish em-bryos. Methods We selected normally developed 4 hpf zygote, and used water bath infecting method to add the drug to the artificial seawater. Each drug had five concentrating groups, a separate control group and solvent control group. We observed the dead zebrafish embryos after 120 hpf drugs, counted the number of deaths and deformities of zebrafish embryos, and cal-culated mortality abnormal rate, the median lethal con-centration (LC50 ), concentration for 50% of maximal effect (EC50 ), therapeutic index (TI) under 120 hpf condition. We also used the formula TI = LC50 / EC50 to calculate positive drug therapeutic index. Based on measured LC50 we calculated most nonlethal concentra-tion (MNLC) of each drug setting, namely 1 / 10 MN-LC, 1 / 3 MNLC, MNLC,LC10 four concentration, tha-lidomide as a positive control, vitamin C as a negative control, artificial seawater as control, 0. 5% DMSO as solvent control. Put in 28. 5 ℃ environment for 120 hours,embryo development was observed daily for de-velopmental state,mortality,deforming rate and abnor-mal condition. Results The result of five drugs LC50 in descending order: cyclophosphamide > azacitidine> tetracycline hydrochloride > acetylsalicylic acid >dexamethasone acetate. EC50 in descending order: cy-clophosphamide > tetracycline hydrochloride > azaciti-dine > acetylsalicylic acid > dexamethasone acetate. The TI values of cyclophosphamide, acetyl salicylic acid, tetracycline hydrochloride, dexamethasone ace-tate, azacitidine were 1. 92, 1. 11, 1. 05, 1. 44, 2. 99, respectively. Conclusion Zebrafish embryo model can be used in the preliminary evaluation of drugs, and the study of early developmental toxicity and safety.

Objective To evaluate the renal toxicity of vancomycin hydrochloride and irbesartan tablets using the zebrafish model. Methods After construction of AB zebrafish kidney model, the fish were treated with drug after fertilization 2 days (2 dpf) to 5 dpf. At the end of the experiment, the number of renal edema zebrafish was counted in each experimental group to evaluate the renal toxicity of drugs. Results The zebrafish development was normal and no obvious toxicity at the dose of 16?4 ng/fish (1/10 MNLD) for vancomycin, and zebrafish renal edema occurred rate was 3?3%, 10% and 10% respectively at the dose of 54?7 ng/fish (1/3 MNLD), 164 ng/fish (MNLD) and 273 ng/fish ( LD10 ) with the death rate of 0%, 0% and 16?7%, respectively, which indicated that there was significant renal toxicity of vancomycin at the dose of 54?7 ng/fish (1/3MNLD) to 273 ng/fish (LD10). Irbesartan didn’t induce renal toxicity at the dose of 8?3 μg/mL (1/10 MNLC) to 91 μg/mL (LC10). Conclusions The zebrafish model of renal toxicity can be used for the early evaluation of drug renal toxicity and we made evaluation of the renal toxicity of vancomycin and irbesartan with this model.

Bone cells live in an environment heavily influenced by mechanical force. The development of bone tissue is dependent on the environment that surrounds it, both in vivo and in vitro. A loading stimulator on research of bone tissue-engineering was developed based on the mechanism of mechanosensation, scaffolding composites with mechanical strains with more physiologic magnitude, frequency components, and waveform. It also achieves the mechanical environment particularly in hard scaffold enough strong like cancellous bone. The device was tested using a reference scaffold made of better elastic plastic material. The experiment results showed that the device could be used in precision strain controls. Since the drive of the stimulator comes from the usage of smart material, piezoceramics, the strain at physiological level is controlled precisely. The stimulator provides a mechanical condition under which the effects of loading applied on bone tissue-engineering culture are conveniently investigated. Furthermore, after the stimulator is improved, it will be an appropriate bioreactor for bone tissue-engineering culture.
Bioreactors ; Bone and Bones ; cytology ; Cell Differentiation ; Cells, Cultured ; Humans ; Mechanotransduction, Cellular ; Osteoblasts ; cytology ; metabolism ; Stress, Mechanical ; Tissue Engineering ; instrumentation ; methods ; Tissue Scaffolds

Objective To study the mechanism of gastric metaplasia in duodenum through the transplantation of a flap of duodenal wall with vascular pedicle to the stomach. Methods The pediculated duodenal wall flaps of Wistar rats were transplanted to their stomachs. The rats were killed at the 3rd, 6th, 9th and 12th month after the operation respectively, and histological changes of the duodenal grafts were observed with optical and electron microscopy.Results Gastric metaplasia was found in the mucosa of duodenal grafts transplanted to the stomach at the 6th, 9th and 12th month.Conclusions The formation of gastric metaplasia in the duodenal mucosa may be related to a change of the microenvironment around the tissues, and duodenal mucosa may differentiate into gastric epithelium by the decrease of pH value.