Objective:To assess the status of undernutrition and appropriate catch-up growth in extremely preterm infants within 24 months of corrected age (CA).Methods:A retrospective cohort study was conducted. A total of 422 extremely preterm infants born at Shenzhen Maternity and Child Healthcare Hospital, Women and Children's Medical Center, Southern Medical University from January 2017 to December 2022 and followed up until 24 months of CA were enrolled. The extremely preterm infants were grouped by gestational age at birth (<25, 25-26, 27 weeks), birth weight (<500, 500-749, 750-999,≥1 000 g), weight for gestational age (large for gestational age (LGA), appropriate for gestational age (AGA), small for gestational age (SGA)) and sex. Weight data within 24 months of CA were collected every 3 months. Nutritional insufficiency, growth rate, and achievement of adequate catch-up growth were analyzed during the period from 0 to 24 months of CA. Z-score method was used to analyze data. Fenton 2013 preterm growth charts (Fenton 2013) were used before 40 weeks of corrected gestational age, and World Health Organization child growth standards (2009) fitted Z-scores were applied from 40 weeks of CA. Changes in weight Z-scores of extremely preterm infants from 0 to 24 months of CA were observed and compared, the occurrence of moderate to severe malnutrition and growth retardation was determined, nutritional insufficiency was assessed, and growth rate as well as the achievement of appropriate catch-up growth were analyzed. The Lambda-mu-sigma method combined with the Z-score fitting model was used to fit and analyze the distribution characteristics of weight percentiles in extremely preterm infants. The Chi-square test was used to compare differences among groups.Results:A total of 422 extremely preterm infants were included, with a gestational age at birth of 26.3(25.4, 27.2) weeks and a birth weight of (880±177) g. Among them, 238 were males and 184 were females; 36 cases (8.5%) were LGA, and 16 cases (3.8%) were SGA. During follow-up within 24 month of CA, 89 cases (21.1%) developed moderate to severe malnutrition. When compared separately among different birth weight and gestational age at birth groups, there had both statistically differences in the incidence of moderate to severe malnutrition ( χ2=42.94 and 9.17, both P<0.05). The incidence was the highest in the birth weight of CA<500 g group and the <25 weeks gestational age at birth group, while it was the lowest in the birth weight of CA≥1 000 g group and the 27 weeks gestational age at birth group in their respective groups. Growth retardation occurred in 5.2% (22/422). However, there had statistically differences in the incidence of growth retardation among different birth weight and gestational age at birth groups, in each grouped time interval ( χ2=21.61 and 4.30, both P<0.05). The proportions of rapid growth were relatively high in the 0-3 months and 3-6 months of CA groups, which were 96 cases (27.4%) and 98 cases (26.6%), respectively. Overall, appropriate catch-up growth was achieved in 341 cases (80.8%) from 0 to 24 months of CA. There had statistically differences in the completion rate of appropriate catch-up growth among different birth weight and gestational age at birth groups ( χ2=23.65 and 7.08, both P<0.05). The completion rate was the highest in the birth weight of CA<500 g group and the <25 weeks of gestational age at birth group, while it was the lowest in the birth weight of CA≥1 000 g group and the 27 weeks of gestational age at birth group. Conclusions:The lower the birth weight and gestational age of extremely preterm infants, the higher the incidence of moderate to severe malnutrition and the lower the achievement rate of adequate catch-up growth within 24 months of CA. The period of 0-6 months of CA is the critical window for catch-up in extremely preterm infants.

Objective:To assess the status of undernutrition and appropriate catch-up growth in extremely preterm infants within 24 months of corrected age (CA).Methods:A retrospective cohort study was conducted. A total of 422 extremely preterm infants born at Shenzhen Maternity and Child Healthcare Hospital, Women and Children's Medical Center, Southern Medical University from January 2017 to December 2022 and followed up until 24 months of CA were enrolled. The extremely preterm infants were grouped by gestational age at birth (<25, 25-26, 27 weeks), birth weight (<500, 500-749, 750-999,≥1 000 g), weight for gestational age (large for gestational age (LGA), appropriate for gestational age (AGA), small for gestational age (SGA)) and sex. Weight data within 24 months of CA were collected every 3 months. Nutritional insufficiency, growth rate, and achievement of adequate catch-up growth were analyzed during the period from 0 to 24 months of CA. Z-score method was used to analyze data. Fenton 2013 preterm growth charts (Fenton 2013) were used before 40 weeks of corrected gestational age, and World Health Organization child growth standards (2009) fitted Z-scores were applied from 40 weeks of CA. Changes in weight Z-scores of extremely preterm infants from 0 to 24 months of CA were observed and compared, the occurrence of moderate to severe malnutrition and growth retardation was determined, nutritional insufficiency was assessed, and growth rate as well as the achievement of appropriate catch-up growth were analyzed. The Lambda-mu-sigma method combined with the Z-score fitting model was used to fit and analyze the distribution characteristics of weight percentiles in extremely preterm infants. The Chi-square test was used to compare differences among groups.Results:A total of 422 extremely preterm infants were included, with a gestational age at birth of 26.3(25.4, 27.2) weeks and a birth weight of (880±177) g. Among them, 238 were males and 184 were females; 36 cases (8.5%) were LGA, and 16 cases (3.8%) were SGA. During follow-up within 24 month of CA, 89 cases (21.1%) developed moderate to severe malnutrition. When compared separately among different birth weight and gestational age at birth groups, there had both statistically differences in the incidence of moderate to severe malnutrition ( χ2=42.94 and 9.17, both P<0.05). The incidence was the highest in the birth weight of CA<500 g group and the <25 weeks gestational age at birth group, while it was the lowest in the birth weight of CA≥1 000 g group and the 27 weeks gestational age at birth group in their respective groups. Growth retardation occurred in 5.2% (22/422). However, there had statistically differences in the incidence of growth retardation among different birth weight and gestational age at birth groups, in each grouped time interval ( χ2=21.61 and 4.30, both P<0.05). The proportions of rapid growth were relatively high in the 0-3 months and 3-6 months of CA groups, which were 96 cases (27.4%) and 98 cases (26.6%), respectively. Overall, appropriate catch-up growth was achieved in 341 cases (80.8%) from 0 to 24 months of CA. There had statistically differences in the completion rate of appropriate catch-up growth among different birth weight and gestational age at birth groups ( χ2=23.65 and 7.08, both P<0.05). The completion rate was the highest in the birth weight of CA<500 g group and the <25 weeks of gestational age at birth group, while it was the lowest in the birth weight of CA≥1 000 g group and the 27 weeks of gestational age at birth group. Conclusions:The lower the birth weight and gestational age of extremely preterm infants, the higher the incidence of moderate to severe malnutrition and the lower the achievement rate of adequate catch-up growth within 24 months of CA. The period of 0-6 months of CA is the critical window for catch-up in extremely preterm infants.

TCM dispensing is the most basic clinical pharmaceutical work of TCM.In recent years,based on the 9 key technologies of TCM dispensing,the TCM dispensing industry has ushered in great development,and innovative TCM dispensing information system and intelligent dispensing equipment have appeared.This article sorted out the current situation of TCM dispensing industry and looked forward to its future development route.The results showed that the introduction of new technology and new equipment in the key technical links of procurement acceptance,dispensing review,TCM decocting,medication guidance and so on have improved the quality of dispensing service and ensured the quality and safety of medication.In the development of modern TCM dispensing industry,it is necessary to improve the quality control standard system,service standard system and core equipment standard system in the standardization of dispensing technology;in terms of talent cultivation in the field of dispensing,it is necessary to focus on restructuring and building new educational models to cultivate new medical talents that intersect medical and engineering fields;in terms of informatization and intelligence,it is necessary to develop intelligent equipment that is more in line with the characteristics of TCM,and further promote and improve the"shared TCM pharmacy"model.Through improving the content of TCM clinical pharmaceutical care,developing new technology and equipment of TCM dispensing,and improving the level of dispensing service and education,it is expected to gradually realize the standardization,informatization and intelligent development of modern TCM dispensing industry.

Objective:To explore the effect of hyperbaric oxygen（HBO）combined with paclitaxel（PTX）on the proliferation，level of reactive oxygen species（ROS），and cell cycle of human gastric cancer AGS cell line cultured in vitro. Methods:After the completion of in vitro culture，the AGS cells were divided into four groups，including control group，PTX group，HBO group，and HBO+PTX group. Cell Counting Kit-8（CCK-8）assay was used to detect the inhibitory effect of HBO combined with PTX on the proliferation of AGS cells. Flow cytometry was adopted to detect the intracellular ROS level and cell cycle arrest. Western blotting was used to assess the expression levels of cell cycle-related proteins. Results:The survival rate of AGS cells in the HBO+PTX group was significantly lower than those in the control group and the HBO group（ P<0.01），and also lower than that in the PTX group（ P<0.05）. The ROS level of AGS cells in the HBO+PTX group was significantly higher than those in the control group and the HBO group（ P<0.01），and also higher than that in the PTX group（ P<0.05）. PTX blocked the AGS cell cycle in S phase（ P<0.05），and the percentage of cells at S phase in the HBO+PTX group was found slightly higher than that in the PTX group（ P>0.05）. The expression level of Cyclin A1 in the HBO+PTX group was significantly lower than those in the other three groups（ P<0.05 or P<0.01）；the expression level of CDK2 in the HBO+PTX group was lower than those in the control group and the HBO group（ P<0.05），and also slightly lower than that in the PTX group（ P>0.05）. Conclusion:HBO can effectively enhance the inhibitory effect of PTX on the proliferation of AGS cells，increase the intracellular ROS level in AGS cells，and block the cell cycle，thereby inhibiting the differentiation and proliferation of AGS cells.

Objective:To explore the effect of hyperbaric oxygen（HBO）combined with paclitaxel（PTX）on the proliferation，level of reactive oxygen species（ROS），and cell cycle of human gastric cancer AGS cell line cultured in vitro. Methods:After the completion of in vitro culture，the AGS cells were divided into four groups，including control group，PTX group，HBO group，and HBO+PTX group. Cell Counting Kit-8（CCK-8）assay was used to detect the inhibitory effect of HBO combined with PTX on the proliferation of AGS cells. Flow cytometry was adopted to detect the intracellular ROS level and cell cycle arrest. Western blotting was used to assess the expression levels of cell cycle-related proteins. Results:The survival rate of AGS cells in the HBO+PTX group was significantly lower than those in the control group and the HBO group（ P<0.01），and also lower than that in the PTX group（ P<0.05）. The ROS level of AGS cells in the HBO+PTX group was significantly higher than those in the control group and the HBO group（ P<0.01），and also higher than that in the PTX group（ P<0.05）. PTX blocked the AGS cell cycle in S phase（ P<0.05），and the percentage of cells at S phase in the HBO+PTX group was found slightly higher than that in the PTX group（ P>0.05）. The expression level of Cyclin A1 in the HBO+PTX group was significantly lower than those in the other three groups（ P<0.05 or P<0.01）；the expression level of CDK2 in the HBO+PTX group was lower than those in the control group and the HBO group（ P<0.05），and also slightly lower than that in the PTX group（ P>0.05）. Conclusion:HBO can effectively enhance the inhibitory effect of PTX on the proliferation of AGS cells，increase the intracellular ROS level in AGS cells，and block the cell cycle，thereby inhibiting the differentiation and proliferation of AGS cells.

Objective To explore the therapeutic effects of comprehensive hyperbaric oxygen therapy (HBO) on cerebral hemorrhage patients in vegetative state (VS) and influential factors on the prognosis of cerebral hemorrhage.Methods Seventy-five cerebral hemorrhage patients who were admitted into the Department of Hyperbaric Oxygen,Beijing Chaoyang Hospital and conformed to the criteria of vegetative state were enrolled for the study.All the patients received 3 to 6 courses of comprehensive HBO therapy and completed 6-month medical follow-ups.Then,improvement of cognition was evaluated before comprehensive HBO therapy,at the 3-month and 6-month medical follow-ups respectively by using the Coma Recovery Scale-Revised (CRS-R).Results At the 3-month and 6-month medical follow-ups,the CRS-R scores were considerably improved as compared with those before treatment,and statistical significance could be seen when comparisons were made between the groups (P ＜ 0.01).At the 6-month follow-up,22 patients regained consciousness,accounting for (29.3％),and 53 patients still had conscious disorder,accounting for 70.7％.With regard to age,gender,causes of cerebral hemorrhage,sites of cerebral hemorrhage,ventricular hemorrhage and courses of HBO therapy,no statistical significance could be noticed in the patients who recovered consciousness at the 6-month medical follow-up,when compared with those who still had conscious disorder(P ＞0.05).As compared with those who had conscious disorder,the CRS-R scores of the patients in the conscious recovery group before treatment were higher.HBO At the 6-month follow-up,the conscious recovery rate of the patients with the amount of hemorrhage equal or lower than 45 ml (40.5％) was obviously higher than that of the patients with the amount of hemorrhage higher than 45 ml (18.4％),and statistical significance could be noted when comparisons were made between them (P ＜ 0.05 or P ＜ 0.01).Logistic regression analysis indicated that the amount of hemorrhage (＞ 45 ml/≤45 ml,OR =8.944,P =0.049),the time of comprehensive HBO therapy (OR =1.182,P =0.006),the CRS-R scores before treatment (OR =0.040,P =0.000) were related with the prognosis of the patients.Conclusion In the treatment of cerebral hemorrhage patients in vegetative state,the amount of hemorrhage,the time of comprehensive HBO therapy and the CRS-R scores before treatment were all influential factors that affected the prognosis of the patients.For this reason,comprehensive HBO therapy should be administered as early as possible.

Objective To explore the therapeutic effects of comprehensive hyperbaric oxygen therapy (HBO) on cerebral hemorrhage patients in vegetative state (VS) and influential factors on the prognosis of cerebral hemorrhage.Methods Seventy-five cerebral hemorrhage patients who were admitted into the Department of Hyperbaric Oxygen,Beijing Chaoyang Hospital and conformed to the criteria of vegetative state were enrolled for the study.All the patients received 3 to 6 courses of comprehensive HBO therapy and completed 6-month medical follow-ups.Then,improvement of cognition was evaluated before comprehensive HBO therapy,at the 3-month and 6-month medical follow-ups respectively by using the Coma Recovery Scale-Revised (CRS-R).Results At the 3-month and 6-month medical follow-ups,the CRS-R scores were considerably improved as compared with those before treatment,and statistical significance could be seen when comparisons were made between the groups (P ＜ 0.01).At the 6-month follow-up,22 patients regained consciousness,accounting for (29.3％),and 53 patients still had conscious disorder,accounting for 70.7％.With regard to age,gender,causes of cerebral hemorrhage,sites of cerebral hemorrhage,ventricular hemorrhage and courses of HBO therapy,no statistical significance could be noticed in the patients who recovered consciousness at the 6-month medical follow-up,when compared with those who still had conscious disorder(P ＞0.05).As compared with those who had conscious disorder,the CRS-R scores of the patients in the conscious recovery group before treatment were higher.HBO At the 6-month follow-up,the conscious recovery rate of the patients with the amount of hemorrhage equal or lower than 45 ml (40.5％) was obviously higher than that of the patients with the amount of hemorrhage higher than 45 ml (18.4％),and statistical significance could be noted when comparisons were made between them (P ＜ 0.05 or P ＜ 0.01).Logistic regression analysis indicated that the amount of hemorrhage (＞ 45 ml/≤45 ml,OR =8.944,P =0.049),the time of comprehensive HBO therapy (OR =1.182,P =0.006),the CRS-R scores before treatment (OR =0.040,P =0.000) were related with the prognosis of the patients.Conclusion In the treatment of cerebral hemorrhage patients in vegetative state,the amount of hemorrhage,the time of comprehensive HBO therapy and the CRS-R scores before treatment were all influential factors that affected the prognosis of the patients.For this reason,comprehensive HBO therapy should be administered as early as possible.

Objective To comprehensively evaluate the clinical efficacy,safety and economic benefits of atorvastatin and fluvastatin in regulating dyslipidemia.Methods The clinical randomized controlled trials (RCTs) for comparing clinical efficacy of atorvastatin and fluvastatin on regulating dyslipidemia were retrieved from databases,including Cochrane Library,PubMed,Medline,Embase,Wiley,Springer,CNKI,Wanfang and VIP,till June 2016.Data were evaluated by two reviewers independently according to the Jadad standard.The changes of low density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C),total cholesterol (TC) and triglyceride (TG) before and after treatment were extracted to perform Meta-analysis by using RevMan5.0 software.The economic evaluation was carried out,as well.Results A total of 7 RCTs were included,including 684 cases of patients treated with fluvastatin and 2 208 cases of patients treated with atorvastatin.The patients were spitted into two subgroups according to the same or different maximum dose of atorvastatin and fluvastatin.The results indicated that the effects of atorvastatin on down-regulating LDL-C,TC and TG levels were significantly better than those of fluvastatin,the differences were statistically significant (Z=23.63、23.32、5.50,P＜0.000 01).No significant difference was found in regulating HDL-C level between atorvastatin and fluvastatin.Conclusion Compared with fluvastatin,atorvastatin is more effective to regulate levels of LDL-C,TC and TG,but there is no significant difference in up-regulating HDL-C level.Additionally,application of atorvastatin is more economicallv effective.

Objective To assess the therapeutic effects of combined hyperbaric oxygen (HBO) in the treatment of unconscious patients after cardiopulmonary resuscitation and also to analyze the relationship between factors associated with HBO in the treatment of conscious disorder and therapeutic effects after cardiopulmonary resuscitation.Methods A retrospective analysis was made on the effects of combined HBO in the treatment of 33 cases of conscious disorder following cardiopulmonary resuscitation from 2002-2012.Patient age,pathogenic factors,courses of HBO treatment and the relationship between treatment time and therapeutic effects were analyzed systematically.Results The effect of comprehensive HBO in the treatment of non-cardiac diseases was obviously superior to that of cardiac diseases(P ＜ 0.05).The therapeutic effect of early treatment (less than 30 days) was obviously better than that of delayed treatment (more than 30 days) (P ＜ 0.05),and patients with a junior age (less than 35 years old) obviously had better therapeutic effects than those with senior age (more than 36 years old) (P ＜ 0.05).Conclusions The therapeutic effect of HBO in the treatment of unconscious patients after cardiopulmonary resuscitation was closely associated with the age,etiology and starting time of HBO therapy.Patients with younger age and non-cardiac diseases and receiving early HBO therapy could achieve better results.

Objective To assess the therapeutic effects of combined hyperbaric oxygen (HBO) in the treatment of unconscious patients after cardiopulmonary resuscitation and also to analyze the relationship between factors associated with HBO in the treatment of conscious disorder and therapeutic effects after cardiopulmonary resuscitation.Methods A retrospective analysis was made on the effects of combined HBO in the treatment of 33 cases of conscious disorder following cardiopulmonary resuscitation from 2002-2012.Patient age,pathogenic factors,courses of HBO treatment and the relationship between treatment time and therapeutic effects were analyzed systematically.Results The effect of comprehensive HBO in the treatment of non-cardiac diseases was obviously superior to that of cardiac diseases(P ＜ 0.05).The therapeutic effect of early treatment (less than 30 days) was obviously better than that of delayed treatment (more than 30 days) (P ＜ 0.05),and patients with a junior age (less than 35 years old) obviously had better therapeutic effects than those with senior age (more than 36 years old) (P ＜ 0.05).Conclusions The therapeutic effect of HBO in the treatment of unconscious patients after cardiopulmonary resuscitation was closely associated with the age,etiology and starting time of HBO therapy.Patients with younger age and non-cardiac diseases and receiving early HBO therapy could achieve better results.