Objective:To investigate the efficacy of rapamycin arterial perfusion combined with 131I-fibroblast activation protein (FAP) loaded dextran microspheres in the treatment of rabbits with liver transplantation tumor. Methods:Fifty male New Zealand white rabbits were selected. Forty white rabbits were used to establish liver transplantation tumor models and were randomly divided into a negative control (based on arterial perfusion with the same volume of normal saline, MO) group, rapamycin arterial perfusion (RA) group, 131I-FAP loaded dextran microspheres for interventional embolization therapy (IF) group, and rapamycin arterial perfusion combined with 131I-FAP-loaded dextran microspheres interventional embolization therapy (RI) group by the random number table method, with 10 rabbits in each group. The remaining 10 unmodeled white rabbits were classified as the normal (based on arterial perfusion with the same volume of normal saline, NO) group. The pathological morphology of tumor tissues was detected by HE staining, liver function was detected by automatic biochemical analyzer, apoptosis of tumor cells was detected by TUNEL method, and the protein expression of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor (VEGFR) in liver tissues was detected by Western blotting. Results:The tumor mass of MO group, RA group, IF group and RI group was (20.33±2.39), (14.62±1.23), (14.34±1.22), (8.28±0.84) g, respectively. Tumor volumes were (0.87±0.13), (0.51±0.09), (0.53±0.08), (0.32±0.02) cm 3, respectively. Tumor necrosis rates were (21.11±2.14) %, (32.18±3.25) %, (32.29±3.28) %, (48.53±4.37) %, respectively. Tumor suppression rates were (0.00±0.00) %, (24.66±2.47) %, (24.13±2.46) %, (45.55±4.51) %, respectively. There were statistically significant differences ( F=316.40, P<0.001; F=159.50, P<0.001; F=356.10, P<0.001; F=571.30, P<0.001); there were statistically significant differences in RA, IF and RI groups compared with the MO group (all P<0.05); there were statistically significant differences between RI group and IF group (all P<0.05). Tumor cells of MO group showed infiltrating growth, with more mitotic images, no obvious necrosis, and a large number of inflammatory cell infiltration. Cancer nests in RA group, IF group and RI group became significantly smaller, tumor cells showed swelling, nuclear contraction and a large number of necrosis, and inflammatory cell infiltration was significantly reduced, among which the improvement was most obvious in RI group. The albumin (ALB) level of NO group, MO group, RA group, IF group and RI group was (40.55±4.38), (17.34±1.02), (22.65±2.18), (22.37±2.17), (29.01±2.83) g/L, respectively. The alanine aminotransferase (ALT) level was (19.68±1.34), (92.17±9.24), (78.71±7.39), (78.35±7.40), (50.30±5.12) U/L, respectively. The aspartate aminotransferase (AST) level was (74.27±7.48), (182.21±20.23), (165.78±16.05), (165.26±16.09), (102.33±11.11) U/L, respectively. The total bilirubin (TBIL) level was (22.42±2.58), (82.24±8.35), (61.86±6.17), (61.53±6.16), (46.45±4.53) μmoL/L, respectively. There were statistically significant differences ( F=105.90, P<0.001; F=189.00, P<0.001; F=99.57, P<0.001; F=142.10, P<0.001); there were statistically significant differences in MO, RA, IF, RI groups compared with the NO group (all P<0.05); there were statistically significant differences in RA, IF, RI groups compared with the MO group (all P<0.05); there were statistically significant differences between IF group and RI group (all P<0.05). The apoptosis rates in MO group, RA group, IF group and RI group were (9.01±1.23) %, (15.65±1.68) %, (15.72±1.69) % and (24.34±2.12) %, respectively, and there was a statistically significant difference ( F=135.30, P<0.001); there were statistically significant differences in RA, IF and RI groups compared with the MO group (all P<0.05); there was a statistically significant difference between RI group and IF group ( P<0.05). VEGF expression level in NO group, MO group, RA group, IF group and RI group was 1.33±0.13, 2.28±0.21, 1.88±0.19, 1.86±0.18 and 1.50±0.14, respectively. VEGFR expression level was 1.32±0.09, 2.14±0.28, 1.91±0.18, 1.89±0.17, 1.62±0.15, respectively. There were statistically significant differences ( F=45.84, P<0.001; F=29.05, P<0.001); there were statistically significant differences in MO, RA, IF, RI groups compared with the NO group (all P<0.05); there were statistically significant differences in RA, IF, RI groups compared with the MO group (all P<0.05); there were statistically significant differences between IF group and RI group (both P<0.05) . Conclusions:Rapamycin arterial perfusion combined with 131I-FAP-loaded dextran microspheres interventional embolization therapy in the treatment of rabbits with liver transplantation tumor can effectively inhibit tumor growth, enhance the tumor necrosis rate, tumor inhibition rate and apoptotic ability, improve liver function indicators, and has a significant therapeutic effect on rabbits with liver transplantation tumor.

Objective:To investigate the diagnostic efficacy of miR-9 and miR-195-3p for primary hepatic carcinoma (PHC), and the changes in miR-9 and miR-195-3p levels after interventional therapy.Methods:A total of 123 cases of PHC patients and 30 cases of liver cirrhosis patients attending Tangshan People's Hospital from May 2019 to May 2020 were selected as the PHC group and the liver cirrhosis group, respectively, and 50 people who were physically healthy during the same period were selected as the healthy group. Serum miR-9 and miR-195-3p levels were detected by real-time quantitative PCR. The relationship between serum miR-9 and miR-195-3p levels and clinical-pathological characteristics of PHC patients was analyzed. Receiver operator characteristic (ROC) curve was applied to analyze the diagnostic efficacy of miR-9 and miR-195-3p for PHC. The changes in serum miR-9 and miR-195-3p levels in PHC patients before and after transcatheter arterial chemoembolization (TACE) were compared.Results:There were statistically significant differences in serum miR-9 (0.99±0.10, 1.31±0.28, 1.68±0.43) and miR-195-3p (0.97±0.10, 0.83±0.22, 0.63±0.18) levels among the healthy group, liver cirrhosis group, and PHC group ( F=69.78, P<0.001; F=74.82, P<0.001), with serum miR-9 levels increased successively and miR-195-3p levels decreased successively among the three groups (all P<0.05). There were statistically significant differences in serum miR-9 ( t=7.45, P<0.001; t=5.32, P<0.001; t=4.96, P<0.001) and miR-195-3p ( t=16.17, P<0.001; t=4.21, P<0.001; t=7.53, P<0.001) levels in PHC patients with different maximum diameters of tumor, clinical stages and degrees of differentiation. ROC curve analysis showed that the area under the curve (AUC) for the combined differential diagnosis of liver cirrhosis and PHC by serum miR-9 and miR-195-3p testing was 0.919, which was higher than the AUC for the differential diagnosis of serum miR-9 (AUC: 0.712, Z=4.38, P<0.001) and miR-195-3p (AUC: 0.844, Z=2.04, P=0.042) alone. After TACE treatment, serum miR-9 levels decreased (1.39±0.21 vs. 1.68±0.43, t=14.22, P<0.001) and miR-195-3p levels increased (0.78±0.22 vs. 0.63±0.18, t=14.84, P<0.001) in patients compared to pre-treatment levels. Conclusion:Serum miR-9 level is increased and miR-195-3p level is decreased in patients with PHC compared with patients with liver cirrhosis and healthy subjects, and the combination of the two has high differential diagnostic efficacy for liver cirrhosis and PHC. After TACE treatment, serum miR-9 level is decreased and miR-195-3p level is increased in PHC patients.

Objective To investigate the early diagnosis value of MR by detecting spinal inflammatory lesions in ankylosing spon-dylitis (AS).Methods Forty patients were involved in this study,including 20 cases with short inflammatory back pain (IBP)histo-ry (duration ≤18 months)and 20 cases with long IBP history (duration ≥24 months).MR images were analyzed retrospectively. Results Patients with a short history of IBP had 7 lesions in vertebral bodies (anterior/posterior spondylitis and spondylodiscitis) and 33 lesions in posterior spinal structures (arthritis of costovertebral joints,costotransversal joints,zygapophyseal joints and en-thesitis of spinal ligaments).Patients with a long history of IBP had 27 lesions in vertebral bodies and 24 lesions in posterior spinal structures.Patients with a short history of IBP had significantly more lesions in posterior spinal structures than in vertebral bodies with 82.5% (33/40)vs 1 7.5% (7/40),respectively (P <0.01).In contrast,patients with a long history of IBP had significantly more inflammation in vertebral bodies with 79.4% (27/34)vs 20.6% (7/34),respectively (P <0.01).Conclusion Inflammatory spinal lesions in patients with a short history of IBP are seen more often in the posterior structures.Early detection of inflammatory spinal lesions by MRI is useful for early diagnosis of AS.