[Objective]Through case analysis,to illustrate the importance of the three methods"open pores","cleaning organs"and"loosen stools"in the treatment of immune-mediated dermatoses.[Methods]Three cases of immune-mediated dermatoses treated by LI Zhengfu were compiled.The etiology,pathogenesis and clinical medication rules at different stages of the disease were analyzed,and the clinical experiences of applying the methods"open pores","clean organs"and"loosen stools"from Neijing were summarized.[Results]The initial pathogenesis of immune-mediated dermatoses involves the stagnation of pathogenic factors on the surface,closure of pores and disharmonious Yingwei.The treatment focus is on the"open pores"method,which involves opening the lung Qi to dispel evil.During the course of the disease,pathological products such as phlegm dampness,water retention and blood stasis may be developed.The treatment focus shifts to the"clean organs"and"loosen stools"methods,aiming to resolve dampness,promote urination and activate blood circulation to eliminate pathogenic factors.Case one was addiction rash(wind cold restricting superficial),treated by dispelling wind and dispersing cold,solving the superficial wet,with Mahuang Xinren Yiyi Gancao Decoction and Guizhi Mahuang Half-Half Decoction;Case two was muscle numbness(triple-Jiao dampness and heat),treated by clearing heat and dampness,relieving triple-Jiao,with revised Haoqin Qingdan Decoction and Sanren Decoction;Case three was psoriasis(hot and toxic blood stasis),treated by clearing heat and cooling blood,dispelling wind and smoothing collateral,with revised Simiao Yong'an Decoction.Three medical cases respectively validated the significant efficacy of the three methods.[Conclusion]The etiology and pathogenesis of immune-mediated dermatoses in traditional Chinese medicine are complex,and the disease course is prolonged.Clinically,flexible application of the three methods"open pores""clean organs"and"loosen stools"for syndrome differentiation and treatment is recommended.

[Objective]Through case analysis,to illustrate the importance of the three methods"open pores","cleaning organs"and"loosen stools"in the treatment of immune-mediated dermatoses.[Methods]Three cases of immune-mediated dermatoses treated by LI Zhengfu were compiled.The etiology,pathogenesis and clinical medication rules at different stages of the disease were analyzed,and the clinical experiences of applying the methods"open pores","clean organs"and"loosen stools"from Neijing were summarized.[Results]The initial pathogenesis of immune-mediated dermatoses involves the stagnation of pathogenic factors on the surface,closure of pores and disharmonious Yingwei.The treatment focus is on the"open pores"method,which involves opening the lung Qi to dispel evil.During the course of the disease,pathological products such as phlegm dampness,water retention and blood stasis may be developed.The treatment focus shifts to the"clean organs"and"loosen stools"methods,aiming to resolve dampness,promote urination and activate blood circulation to eliminate pathogenic factors.Case one was addiction rash(wind cold restricting superficial),treated by dispelling wind and dispersing cold,solving the superficial wet,with Mahuang Xinren Yiyi Gancao Decoction and Guizhi Mahuang Half-Half Decoction;Case two was muscle numbness(triple-Jiao dampness and heat),treated by clearing heat and dampness,relieving triple-Jiao,with revised Haoqin Qingdan Decoction and Sanren Decoction;Case three was psoriasis(hot and toxic blood stasis),treated by clearing heat and cooling blood,dispelling wind and smoothing collateral,with revised Simiao Yong'an Decoction.Three medical cases respectively validated the significant efficacy of the three methods.[Conclusion]The etiology and pathogenesis of immune-mediated dermatoses in traditional Chinese medicine are complex,and the disease course is prolonged.Clinically,flexible application of the three methods"open pores""clean organs"and"loosen stools"for syndrome differentiation and treatment is recommended.

Objective:To investigate the clinical characteristics, diagnosis and treatment status, and existing problems of early infantile epileptic encephalopathy type 2 (EIEE2) caused by de novoa mutation of cyclin-dependent kinase-like 5 gene (CDKL5).Methods:The medical history, auxiliary examination and diagnosis and treatment characteristics of 1 case with EIEE2 caused by de novoa mutation of CDKL5 gene in neonatal department of Children′s Hospital of Nanjing Medical University on August 12, 2019 were retrospectively analyzed.Combined with relevant literatures, the clinical diagnosis and treatment ideas and future prospects of this disease were summarized.Results:The patient was a female child with the age of 13 days and 23 hours.The main clinical manifestation was recurrent convulsion which was not alleviated significantly after using antiepileptic drug.The second-generation sequencing detected c. 119C>T/ p. A40V heterozygous mutation of CDKL5 gene, which was de novo mutation.Conclusions:EIEE2 caused by de novoa mutation of CDKL5 gene is a rare disease worthy of attention.Early detection and genetic diagnosis are the key to improve the diagnosis and treatment rate.

Endometrial cancer ( EC) is one of the most common gynecological cancer ,of which the molecular biolog-ical mechanisms is very complex .In recent years, it has been reported that the abnormally regulated PI 3K/Akt/mTOR signaling pathway is significantly related with endometrial cancer .The mutation and abnormal activation of the receptors and kinase of the PI 3 K/Akt/mTOR signaling pathway is a potential therapeutic target of endometrial cancer .