Objective:To explore the effects of a method combining the knowledge, attitude, and practice (KAP) model with objective structured clinical examination (OSCE) on soft skill evaluation score, training satisfaction, personal growth, and radiation effect in training perinatal specialist nurses, and to evaluate the effectiveness of this method in improving the comprehensive clinical nursing ability and training effect of perinatal specialist nurses.Methods:Using the cluster sampling non-random grouping method, 63 trainees who received training at the Perinatal Specialist Nurse Training Base of Chongqing Maternal and Child Health Hospital from 2019 to 2020 were selected as the control group, and the conventional theoretical and clinical practice training and assessment methods were adopted. Additionally, 60 trainees who received training from 2021 to 2022 were selected as the experimental group, and the training method combining OSCE and KAP was adopted. General information questionnaire, training assessment satisfaction questionnaire, and a nursing soft skill evaluation scale were used for investigation. SPSS 25.0 software was used for descriptive analysis. The independent samples t-test and chi-square test were used to compare the differences in soft skill evaluation score, training satisfaction, personal growth, and radiation effect between the two groups of trainees after training. Results:The average difficulty of OSCE was 0.7 and the average discrimination was 0.5. The total score of soft skill evaluation in the experimental group was significantly higher than that in the control group ( t=3.73, P<0.001). After training, the total satisfaction score of the experimental group was (30.95±5.36), which was significantly higher than that of the control group (28.73±4.72) ( t=2.44, P=0.016). There were no significant differences in personal growth and radiation effect between the two groups after training ( P>0.05). Conclusions:The combination of KAP and OSCE in training perinatal specialist nurses is helpful to improve the trainees' soft skills and training satisfaction, and is worthy of promotion and application in clinical specialist nurse training.

Objective:To explore the effects of a method combining the knowledge, attitude, and practice (KAP) model with objective structured clinical examination (OSCE) on soft skill evaluation score, training satisfaction, personal growth, and radiation effect in training perinatal specialist nurses, and to evaluate the effectiveness of this method in improving the comprehensive clinical nursing ability and training effect of perinatal specialist nurses.Methods:Using the cluster sampling non-random grouping method, 63 trainees who received training at the Perinatal Specialist Nurse Training Base of Chongqing Maternal and Child Health Hospital from 2019 to 2020 were selected as the control group, and the conventional theoretical and clinical practice training and assessment methods were adopted. Additionally, 60 trainees who received training from 2021 to 2022 were selected as the experimental group, and the training method combining OSCE and KAP was adopted. General information questionnaire, training assessment satisfaction questionnaire, and a nursing soft skill evaluation scale were used for investigation. SPSS 25.0 software was used for descriptive analysis. The independent samples t-test and chi-square test were used to compare the differences in soft skill evaluation score, training satisfaction, personal growth, and radiation effect between the two groups of trainees after training. Results:The average difficulty of OSCE was 0.7 and the average discrimination was 0.5. The total score of soft skill evaluation in the experimental group was significantly higher than that in the control group ( t=3.73, P<0.001). After training, the total satisfaction score of the experimental group was (30.95±5.36), which was significantly higher than that of the control group (28.73±4.72) ( t=2.44, P=0.016). There were no significant differences in personal growth and radiation effect between the two groups after training ( P>0.05). Conclusions:The combination of KAP and OSCE in training perinatal specialist nurses is helpful to improve the trainees' soft skills and training satisfaction, and is worthy of promotion and application in clinical specialist nurse training.

High-mobility group box-1 (HMGB1) is a member of the high-mobility group proteins and is present in eukaryotic cells. HMGB1 is not only a nuclear protein but also a pro-inflammatory factor, and the increase in HMGB1 level in the body indicates cell destruction and inflammatory response. Hepatitis B virus (HBV) infection is a major cause of chronic hepatitis B, liver cirrhosis, and hepatocellular carcinoma in the world. In recent years, the role of HMGB1 in HBV-related liver diseases has attracted more and more attention, especially the important role of HMGB1 in the progression of liver inflammation and liver cancer. This article reviews the recent research advances in the role of HMGB1 in the development, progression, and treatment of HBV-related liver diseases.

Objective To construct the short hairpin RNA recombinant plasmids targeting mdr1 gene which expresses highly in ovarian cancer resistance strain SKOV3/TAXOL to silence endogenefic mdr1 gene expression and investigate the role of mdr1 gene in the development of resistant ovarian cancer. Methods The pGPU6/GFP/Neo-mdr1 were constructed by gene clone technology. The influence on proliferation and apoptosis were investigated by CCK-8 in SKOV3/TAXOL after transfected pGPU6/GFP/Neo-mdr1. Results The expression against mdr1 proteins were inhibited by pGPU6/GFP/Neo-mdr1. The cell proliferation were inhibited after transfected pGPU6/GFP/Neo-mdr1 by CCK-8. The apoptosis were observed in DAB experiments and the apoptosis rate increased. Conclusion mdr1 plays an important role in proliferation of resistant ovarian cancer and the short hairpin RNA of mdr1 can efficiently suppress mdr1 expression and enhance the apoptosis in SKOV3/ATAXOL.

Objective To study the relation between the expression of drug-resistance gene and in vitro drug sensitivity test in ovarian carcinoma and clinical biological behavior.Methods In 60 primarily treated patients with epithelial ovarian carcinoma,the expression of COX-2,Connexin43(CX43)and P-gp was detected using flow cytometry.Sensitivity of cells of short term in vitro culture to anticancer drugs was also examined by the game techniques.Remits The expression level of COX-2 was higher in tissue with DDP-resistance patients than those with sensitivity patients but the expression level of CX43 was lower in tissue with DDP-resistance and Taxol-resistanee patients than those with sensitivity patients.The expression level of P-gp was higher in tissue with VP16-resistance patients than those with sensitivity patients.The sensitivity of DDP,Taxol and VP16 was lower in tissue with higher expression of COX-2 and P-gp than those with lower expression of COX-2 and P-gp.The sensitivity of DDP and Taxol was higher in tissue with higher expression of CX43 than those with lower expression of CX43.Conclusion The in vitro sensitivity of some of the drugs is effected by tissues with expression of COX-2,P-gp and CX43.It is not only useful but also individualized treatment for application of sensitive drug test and detecting the expression of COX-2,P-gP and CX43 to increase the response rate of chemotherapy in patients with epithelial ovarian carcinoma.