Objective To explore the effects of different eating methods(tube feeding,esophageal stents,intravenous nutrition)on the survival of the patients with esophageal perforation after esophageal squamous cell carcinoma radiotherapy.Methods A retrospective analysis was conducted on the clinical data of 61 inpatients with esophageal perforation after esophageal squamous cell carcinoma radiotherapy in Nanyang Municipal First People's Hospital from January 2013 to December 2022.According to the different perforation sites,they were divided into the esophagomediastinal fistula group(n=38)and esophagotracheal fistula group(n=23).The Kaplan-Meier method was used to evaluate the survival situation of the patients with different eating patterns,and the nutritional status of the patients one in 1 month after esophageal perforation was eval-uated.Results All patients died within 12 months(median survival time was 4.3 months).The 3-month and 6-month survival rates of the patients with tube feeding were 81.2％and 37.5％respectively,those of the pa-tients with esophageal stents were 63.2％and 15.8％,and those of the patients with intravenous nutrition were 40.0％and 0 respectively,and the differences were statistically significant(P＜0.05).In the esophago-mediastinal fistula group,the 3-month and 6-month survival rates of the patients with tube feeding were 85.0％and 45.0％respectively,those of patients with esophageal stents were 75.0％and 16.7％respectively,and those of the patients with intravenous nutrition were 50.0％and 0 respectively,and the differences were statistically significant(P＜0.05).In the esophagotracheal fistula group,the 3-month and 6-month survival rates of the patients with tube feeding were 75.0％and 25.0％respectively,those of the patients with esopha-geal stents were 42.9％and 14.3％respectively,and those of the patients with intravenous nutrition were 25.0％and 0 respectively,and the differences were not statistically significant(P＞0.05).The nutritional sta-tus score of the patients with intravenous nutrition was significantly higher than that of the patients with tube feeding and esophageal stents(P＜0.05),but there was no statistically significant difference in the nutritional status score between the patients with tube feeding and the patients with esophageal stents(P＞0.05).Conclusion Com-pared with esophageal stents and intravenous nutrition,tube feeding could achieve relatively higher survival rates and better nutritional status.

The hypoxanthine-guanine phosphoribosyl transferase (HGPRT) gene mutation is responsible for gouty arthritis, kidney stone, and Lesch-Nyhan Syndrome (LNS). It has been reported that the expression of HGPRT is decreased or even absent in these diseases. Rabbits are an ideal model for studying the pathology of these diseases. Therefore, the development of an HGPRT-knockdown rabbit model will be highly beneficial m such studies. Stable HGPRT-knoekdown transgenie fibroblast lines were generated by transfecting rabbit fibroblasts with RNA interference (RNAi) plasmids. Polymerase chain reaction (PCR) analyses indicated that the average positive rate was 83.3%. The mRNA and protein levels of HGPRT in the transgenic fibroblast lines were significantly lower than that in the control. Transgenic rabbit blastocysts were derived after performing nuclear transfer. The results show that RNAi can be used to stably knock down expression of the HGPRT in rabbit fibroblasts and further improvements in related technologies will facilitate the use of this method for the generation of HGPRT-knockdown rabbits.

Objective Self-made docetaxel-carrying microbubbles were developed and evaluated as a new ultrasound contrast agent for diagnosis and targeted-chemotherapeutic drug delivery.Methods Docetaxel was mixed with an aqueous suspension of phospholipids in vials,which were put into 40℃ water for 30 minutes,after cooling,gas in vials was replaced with perfluoropropane gas,then vials were agitated for 45 s on a shaking device.Properties were studied contained concentration,size,zeta potential,drug entrapment efficiency and drug-loading amount.Drug released with ultrasound and imaging for VX2 carcinoma in rabbits were observed.Results Docetaxel-carrying microbubbles had a concentration of approximately 2.2×109～3.2×109/ml,a mean size of 623.1 am and a zeta potential of -(3.1±0.9)mV.Size distribution was 473.4～706.6 nm.Drug entrapment efficiency was more than 70% and drug-loading amount was(17.5±0.8)%.Fixed amount of ultrasound energy ruptured microbubbles and released docetaxel.Liver imaging of rabbits could be enhanced obviously and persistently,"fast in and out"phenomena was typical of VX2 carcinoma.Conclusions Liposome microbubbles represent a new class of acoustically active drug delivery vehicles.Docetaxel-carrying microbubbles can promote the value of ultrasound in tumor diagnosis,and docetaxel-carrying microbubbles combined with ultrasound have a hope to establish a kind of real-time monitoring,targeted system for tumor therapy.