Chronic refractory wound (CRW) is one of the most challengeable issues in clinic due to complex pathogenesis, long course of disease and poor prognosis. Experts need to conduct systematic summary for the diagnosis and treatment of CRW due to complex pathogenesis and poor prognosis, and standard guidelines for the diagnosis and treatment of CRW should be created. The Guideline forthe diagnosis and treatment of chronic refractory wounds in orthopedic trauma patients ( version 2023) was created by the expert group organized by the Chinese Association of Orthopedic Surgeons, Chinese Orthopedic Association, Chinese Society of Traumatology, and Trauma Orthopedics and Multiple Traumatology Group of Emergency Resuscitation Committee of Chinese Medical Doctor Association after the clinical problems were chosen based on demand-driven principles and principles of evidence-based medicine. The guideline systematically elaborated CRW from aspects of the epidemiology, diagnosis, treatment, postoperative management, complication prevention and comorbidity management, and rehabilitation and health education, and 9 recommendations were finally proposed to provide a reliable clinical reference for the diagnosis and treatment of CRW.

Purpose: Isocitrate dehydrogenase 1 (IDH1) mutations are the most common genetic abnormalities in low-grade gliomas and secondary glioblastomas. Glioma patients with these mutations had better clinical outcomes. However, the effect of IDH1 mutation on drug sensitivity is still under debate. Materials and Methods: IDH1-R132H mutant cells were established by lentivirus. IDH1-R132H protein expression was confirmed by western blot. The expression of ataxia telangiectasia mutated (ATM) signaling pathway and apoptosis-related proteins were detected by immunofluorescence and western blot. Temozolomide (TMZ) induced cell apoptosis was detected by flow cytometry. Tumor cell proliferation was detected by Cell Counting Kit-8. In vivo nude mice were used to confirm the in vitro roles of IDH1 mutation. Results: We established glioma cell lines that expressed IDH1-R132H mutation stably. We found that TMZ inhibited glioma cells proliferation more significantly in IDH1 mutant cells compared to wild type. The IC50 of TMZ in IDH1-R132H mutant group was less than half that of wild-type group (p < 0.01). TMZ significantly induced more DNA damage (quantification of γH2AX expression in IDH1 mutation vs. wild type, p < 0.05) and apoptosis (quantification of AnnexinV+propidium iodide–cells in IDH1 mutation versus wild type, p < 0.01) in IDH1 mutant gliomas compared to wild-type gliomas. The ATM-associated DNA repair signal was impaired in IDH1 mutant cells. Inhibiting the ATM/checkpoint kinase 2DNA repair pathway further sensitized IDH1 mutant glioma cells to chemotherapy. We found that IDH1 mutation significantly inhibited tumor growth in vivo (the tumor size was analyzed statistically, p < 0.05). Moreover, we confirmed that gliomas with IDH1 mutation were more sensitive to TMZ in vivo compared to wild type significantly and the results were consistent with the in vitro experiment. Conclusion These results provide evidence that combination of TMZ and ATM inhibitor enhances the antitumor effect in IDH1 mutant gliomas.

Purpose: Isocitrate dehydrogenase 1 (IDH1) mutations are the most common genetic abnormalities in low-grade gliomas and secondary glioblastomas. Glioma patients with these mutations had better clinical outcomes. However, the effect of IDH1 mutation on drug sensitivity is still under debate. Materials and Methods: IDH1-R132H mutant cells were established by lentivirus. IDH1-R132H protein expression was confirmed by western blot. The expression of ataxia telangiectasia mutated (ATM) signaling pathway and apoptosis-related proteins were detected by immunofluorescence and western blot. Temozolomide (TMZ) induced cell apoptosis was detected by flow cytometry. Tumor cell proliferation was detected by Cell Counting Kit-8. In vivo nude mice were used to confirm the in vitro roles of IDH1 mutation. Results: We established glioma cell lines that expressed IDH1-R132H mutation stably. We found that TMZ inhibited glioma cells proliferation more significantly in IDH1 mutant cells compared to wild type. The IC50 of TMZ in IDH1-R132H mutant group was less than half that of wild-type group (p < 0.01). TMZ significantly induced more DNA damage (quantification of γH2AX expression in IDH1 mutation vs. wild type, p < 0.05) and apoptosis (quantification of AnnexinV+propidium iodide–cells in IDH1 mutation versus wild type, p < 0.01) in IDH1 mutant gliomas compared to wild-type gliomas. The ATM-associated DNA repair signal was impaired in IDH1 mutant cells. Inhibiting the ATM/checkpoint kinase 2DNA repair pathway further sensitized IDH1 mutant glioma cells to chemotherapy. We found that IDH1 mutation significantly inhibited tumor growth in vivo (the tumor size was analyzed statistically, p < 0.05). Moreover, we confirmed that gliomas with IDH1 mutation were more sensitive to TMZ in vivo compared to wild type significantly and the results were consistent with the in vitro experiment. Conclusion These results provide evidence that combination of TMZ and ATM inhibitor enhances the antitumor effect in IDH1 mutant gliomas.

Objective In our previous study, we had generated various zebrafish mutant lines with tissue?specific GFP expression by Tol2 transposon?mediated insertional mutagenesis. Among these mutants,the Tol2:20141221t line ex?presses GFP in nervous system, while the position within zebrafish genome where transposon inserted has not yet been iden?tified. The aim of this study was to identify and analyze this genetically modified mutant. Methods The transgenic inser?tion loci in the genome of Tol2:20141221t line was identified byTAIL?PCR and the spatial and temporal expression profile of the affected gene was examined by in situ hybridization. Homozygous mutant of Tol2:20141221t was generated for explo?ring related developmental defects. Results Tol2 transposon was inserted into the 8th intron region of sat1.a gene, and in?duced premature transcription termination. The maternal and zygotic mutants of Tol2:20141221t was generated, while with?out apparent developmental defects. Conclusions We have generated and identified the zebrafishsat1.a mutant mediated by Tol2 transposon. This gene insertion mutant exhibits no obvious developmental abnormalities, but may serve as a power?ful tool to study the development of nervous system.

Objective To conduct a preliminary analysis on HBO research in China from 2010 to 2014,so as to provide evidence for the development of the discipline.Methods Information retrieval was made by using Chinese Biomedical Disk (CBM) as the chief source of information.Through the approach of bibliometrics,analyses were made on the year distributions of literature,the amount of literature published within the years,the regional distributions of authors,institutions and related journals concerning HBO research from 2010 to 2014.Results The amount of papers published from 2010 to 2013 was generally stable,but with a slight decrease in 2014.Analyses of regional distribution of authors indicated a downward trend from the higher distribution in the east coastal areas to the lower distribution in the mid-west inland areas.Nine institutions that have more than 15 contributions within the said years are basically located in Beijing,Shanghai,Guangzhou and other highly developed coastal and river basin areas with rich water resources,with hospitals as the main research bodies.Subject analyses suggested that researches concerning HBO research were mainly focused on the toxication of carbon monoxide,the treatment of such diseases as cerebral infarction,craniocerebral injury,sudden hearing loss,diseases of newborns,coma,loss of consciousness,cerebral ischemia,spinal cord injury,cerebral hemorrhage,diabetes foot,etc.,as well as its application in the rehabilitation of patients following surgery.The Chinese Journal of Nautical Medicine and Hyperbaric Medicine as the core periodical in the field of HBO research,is the one that has the largest amount of papers published within the years,with the medical journals on neural diseases and rehabilitation coming next.Conclusions Great progress has been made in the research and application of HBO in recent years,which is mainly manifested in the treatment of diseases that are more susceptible to HBO therapy.However,most papers are on clinical applications,few papers are associated with basic research.On the other hand,significant differences could be noted in the regional distribution of author population and research institutions.

Objective To conduct a preliminary analysis on HBO research in China from 2010 to 2014,so as to provide evidence for the development of the discipline.Methods Information retrieval was made by using Chinese Biomedical Disk (CBM) as the chief source of information.Through the approach of bibliometrics,analyses were made on the year distributions of literature,the amount of literature published within the years,the regional distributions of authors,institutions and related journals concerning HBO research from 2010 to 2014.Results The amount of papers published from 2010 to 2013 was generally stable,but with a slight decrease in 2014.Analyses of regional distribution of authors indicated a downward trend from the higher distribution in the east coastal areas to the lower distribution in the mid-west inland areas.Nine institutions that have more than 15 contributions within the said years are basically located in Beijing,Shanghai,Guangzhou and other highly developed coastal and river basin areas with rich water resources,with hospitals as the main research bodies.Subject analyses suggested that researches concerning HBO research were mainly focused on the toxication of carbon monoxide,the treatment of such diseases as cerebral infarction,craniocerebral injury,sudden hearing loss,diseases of newborns,coma,loss of consciousness,cerebral ischemia,spinal cord injury,cerebral hemorrhage,diabetes foot,etc.,as well as its application in the rehabilitation of patients following surgery.The Chinese Journal of Nautical Medicine and Hyperbaric Medicine as the core periodical in the field of HBO research,is the one that has the largest amount of papers published within the years,with the medical journals on neural diseases and rehabilitation coming next.Conclusions Great progress has been made in the research and application of HBO in recent years,which is mainly manifested in the treatment of diseases that are more susceptible to HBO therapy.However,most papers are on clinical applications,few papers are associated with basic research.On the other hand,significant differences could be noted in the regional distribution of author population and research institutions.

Objective To get a more comprehensive knowledge of the Chinese Journal of Nautical Medicine and Hyperbaric Medicine through analysis of the bibliometric data published in the journal from 2009 -2013,so that readers will have a profound knowledge of the journal and it will also facilitate the editorial board to improve the academic level of the journal by drawing experience from others.Methods With the scientific papers published in the journal from 2009-2013 as research subjects,in reference to " the Citation Report of the China Science and Technology Journals" between the years of 2010-2014,and through the approach of bibliometrics,statistical analyses were made on the amount of papers published,the citation frequency,influential factors,foundation thesis ratio,regional distribution and other data in recent 5 years.Results With regard to the amount of papers published,the yearly amount tended to decrease year by year,but with that of 2013 increased slightly.(2)Concerning citation frequency,total citation frequency on the whole tended to increase year after year,i.e.the yearly index of 2011 increased considerably,but the indexes of 2012-2013 tended to be stable.(3)With respect to influential factors,the factors derived from the 2000-2011 issues were relatively low,but the influential factors in the 2012 and 2013 issues were increased quite considerably.(4)With respect to foundation thesis ratio,the highest value of 2012 was 0.38,while the lowest value of 2013 was 0.128.As could be seen,there was great fluctuation.(5)Concerning regional distribution,the papers published in the journal in the recent 5 years were from about 90 institutions or organizations of 20 provinces,autonomous regions and municipalities directly under the central government.(6)The average number of authors per paper each year was over 4.Conclusions The Chinese Journal of Nautical Medicine and Hyperbaric Medicine is the only core periodical in the fields of nautical medicine,hyperbaric oxygen and diving medicine,and is also an important platform for physicians,scholars and research workers to exchange knowledge and experience in these fields.Bibliometric statistics and analysis of the papers published in the recent 5 years definitely reflect the development status and trend of the journal,which is of great significance to better improvement in the work of the editorial department and further progress in development of the discipline as well.

Objective To get a more comprehensive knowledge of the Chinese Journal of Nautical Medicine and Hyperbaric Medicine through analysis of the bibliometric data published in the journal from 2009 -2013,so that readers will have a profound knowledge of the journal and it will also facilitate the editorial board to improve the academic level of the journal by drawing experience from others.Methods With the scientific papers published in the journal from 2009-2013 as research subjects,in reference to " the Citation Report of the China Science and Technology Journals" between the years of 2010-2014,and through the approach of bibliometrics,statistical analyses were made on the amount of papers published,the citation frequency,influential factors,foundation thesis ratio,regional distribution and other data in recent 5 years.Results With regard to the amount of papers published,the yearly amount tended to decrease year by year,but with that of 2013 increased slightly.(2)Concerning citation frequency,total citation frequency on the whole tended to increase year after year,i.e.the yearly index of 2011 increased considerably,but the indexes of 2012-2013 tended to be stable.(3)With respect to influential factors,the factors derived from the 2000-2011 issues were relatively low,but the influential factors in the 2012 and 2013 issues were increased quite considerably.(4)With respect to foundation thesis ratio,the highest value of 2012 was 0.38,while the lowest value of 2013 was 0.128.As could be seen,there was great fluctuation.(5)Concerning regional distribution,the papers published in the journal in the recent 5 years were from about 90 institutions or organizations of 20 provinces,autonomous regions and municipalities directly under the central government.(6)The average number of authors per paper each year was over 4.Conclusions The Chinese Journal of Nautical Medicine and Hyperbaric Medicine is the only core periodical in the fields of nautical medicine,hyperbaric oxygen and diving medicine,and is also an important platform for physicians,scholars and research workers to exchange knowledge and experience in these fields.Bibliometric statistics and analysis of the papers published in the recent 5 years definitely reflect the development status and trend of the journal,which is of great significance to better improvement in the work of the editorial department and further progress in development of the discipline as well.