Objective To explore the influencing factors of median nerve entrapment syndrome(MNES)in patients with diabetic peripheral neuropathy(DPN).Methods A total of 150 patients with type 2 diabetes mellitus(T2DM)who were hospitalized in the Department of Endocrinology in our hospital from May 2022 to May 2024 were selected and divided into the simple T2DM group,the DPN group and the combined MNES group,with 50 cases in each group.The general data,biochemical indicators and median nerve ultrasound characteristics of the three groups were compared.Logistic regression was used to analyze the influencing factors of MNES in patients with DPN.Results The DM duration,low density lipoprotein cholesterol(LDL-C),total cholesterol(TC),fasting plasma glucose(FPG),glycated albumin(GA),hemoglobin A1c(HbA1c),the transverse diameter(d1),anteroposterior diameter(d2),cross-sectional area and the proportion of blurred"sieve-like"structure,decreased nerve bundle echo,thickened epineurium,and unclear boundary with adjacent tissues in the T2DM,DPN,and MNES groups increased in turn(P<0.05),and the levels of vitamin D,glutathione(GSH)and superoxide dismutase(SOD)decreased in turn(P<0.05).The hip circumference and diastolic blood pressure in DPN and MNES groups were higher than those in T2DM group(P<0.05).Logistic regression analysis showed that the DM duration,LDL-C,TC,FPG,GA,HbA1c,GSH,SOD,d1,d2,cross-sectional area,blurred"sieve-like"structure,decreased echo of nerve tracts,thickening of the outer nerve membrane,and unclear boundaries with adjacent tissues were the influencing factors for the occurrence of MNES in patients with DPN.Conclusions The occurrence of MNES in patients with DPN is affected by multiple factors.Regular monitoring of relevant indicators and ultrasound features of the median nerve can help to identify and intervene early to delay the development of MNES in patients with T2DM.

Objective To explore the influencing factors of median nerve entrapment syndrome(MNES)in patients with diabetic peripheral neuropathy(DPN).Methods A total of 150 patients with type 2 diabetes mellitus(T2DM)who were hospitalized in the Department of Endocrinology in our hospital from May 2022 to May 2024 were selected and divided into the simple T2DM group,the DPN group and the combined MNES group,with 50 cases in each group.The general data,biochemical indicators and median nerve ultrasound characteristics of the three groups were compared.Logistic regression was used to analyze the influencing factors of MNES in patients with DPN.Results The DM duration,low density lipoprotein cholesterol(LDL-C),total cholesterol(TC),fasting plasma glucose(FPG),glycated albumin(GA),hemoglobin A1c(HbA1c),the transverse diameter(d1),anteroposterior diameter(d2),cross-sectional area and the proportion of blurred"sieve-like"structure,decreased nerve bundle echo,thickened epineurium,and unclear boundary with adjacent tissues in the T2DM,DPN,and MNES groups increased in turn(P<0.05),and the levels of vitamin D,glutathione(GSH)and superoxide dismutase(SOD)decreased in turn(P<0.05).The hip circumference and diastolic blood pressure in DPN and MNES groups were higher than those in T2DM group(P<0.05).Logistic regression analysis showed that the DM duration,LDL-C,TC,FPG,GA,HbA1c,GSH,SOD,d1,d2,cross-sectional area,blurred"sieve-like"structure,decreased echo of nerve tracts,thickening of the outer nerve membrane,and unclear boundaries with adjacent tissues were the influencing factors for the occurrence of MNES in patients with DPN.Conclusions The occurrence of MNES in patients with DPN is affected by multiple factors.Regular monitoring of relevant indicators and ultrasound features of the median nerve can help to identify and intervene early to delay the development of MNES in patients with T2DM.

Objective To compare two effective preparation methods for paclitaxel-loaded lipid microbubbles, and to evaluate the physiochemical properties as for acoustic activated drug delivery. Methods Paclitaxel-loaded lipid microbubbles were prepared with two methods: one was mixed with phospholipids directly (Ⅰ); the other was added in triacetin, then mixed with phospholipids (Ⅱ). Concent ration, size, pH, drug entrapment efficiency, drug-loading amounts of these two kinds of paclitaxel-loaded lipid microbubbles were studied, while drug release with ultrasound and tumor imaging enhanced on rabbit breast tumor were observed. Results There was no significant difference in tumor imaging between two kinds of microbubbles which could be ruptured by low energy ultrasound. Compared with Ⅰ, the mean diameter of Ⅱ decreased significantly ([ 1.07±0.38] μm vs [2.79± 0.41] μm, P<0.01), the surface potencial was higher ([19.10±0.32] mV vs [-5.90±0.21] mV, P<0.01), whereas entrapment efficiency and drug-loading amounts increased markedly ([ 95.00±1.22]% vs [36.10±4.74]%, P<0.01; [5.60±0.11]% vs [0.50±0.04]%, P<0.01). Conclusion The Ⅱ paclitaxel-loaded lipid microbubbles added triacetin have an important clinical value.

Objective To study the dynamic changes of transendothelial electrical resistance (TEER) on our in vitro model of brain blood barrier (BBB) made from primary culture of BALB/c mouse brain microvascular endothelial cells (BMVEC), and to explore the relation between TEER and BBB permeability, and search for the best culture condition. Methods BMVEC were isolated from BALB/c mouse and cultured on a transwell insert with special micro-pore. The cells were identified with immunohistochemical methods and electron microscope. TEER over BMVEC was measured after BBB model establishment for determining the 3H-Glucose permeability of BBB in vitro. Results BMVEC cultured in the transwell insert exhibited typical "flagstone" appearance and in a tight monolayer structure under electron microscope. Immunohistochemical detection of ZO-1 protein, a marker antigen of tight junction, showed smooth, continuous and tight junctions between confluent BMVEC. TEER over BMVEC monolayer increased to (346?10) ?/cm2 when the permeability for 3H-Glucose was decreased to the minimum. Conclusion BBB model in vitro made from primary culture of BMVEC in transwell has the basic characteristics of BBB in morphology, electrical resistance and permeability.