Objective To analyze the chemical composition of Porphyra haitanensis cultivated in Fujian Province.Methods The contents of crude protein,carbohydrate,crude fat,ash,free amino acids,inorganic elements,chlorophyll a,carotenoid,phycoerythrin and phycocyanin of dried P.haitanensis were determined by routine analysis methods.Results It showed the contents of crude protein,carbohydrate,crude fat and ash of dried P.haitanensis were 34.88%～37.21%,46.86%～49.27%,1.68%～2.09%and 8.75%～9.92%.The contents of chlorophyll a,carotenoid,phycoerythrin and phycocyanin in P.haitanensis were 60 1～646,120～130,1289～1802,1255～1743mg(100g)-1.P.haitanensis is rich in free amino acids and inorganic elements,i.e.K,Na,Mg,Fe,P and Zn,but very little heavy metals,i.e.Cu,Cd and Cr.Conclusions The P.haitanensis was a kind of health food,which was in high content of protein,low content of fat and rich of inorganic elements.

Objective To study the active secondary metabolites from marine microorganism FIM02-635. Methods The producing strain was identified by taxonomical and phylogenetic studies. Two compounds FW635I1 and FW635I2 with immunosuppressive activities were extracted by organic solvents from the culture broth and purified by silica gel column chromatography and high speed counter current chromatography. The structures of the two compounds were determined by physico-chemical properties and spectral analyses,the biological activities were assayed in vitro. Results and Conclusion The producing strain was named as Micromonospora carbonacea FIM 02-635. Two compounds FW635I1 and FW635I2 were determined to be isoflavone Daidzein and Genistein, respectively, showed immunosuppressive and antitumor activities, but not antimicrobial activities.

Objective To detect the antiviral effect and its antiviral mechanism of the sulfated polysaccharide SP2 isolated from the brown alga Sargassum patens C. Ag. against RSV.Methods The antiviral activity,cytotoxicity and its possible antiviral mechanism were assayed by cytopathogenic effect (CPE) inhibition assay,MTT assay and plaque reduction assay. Reduction assay when it was added during virus adsorption. It did not show any antiviral activity against influenza A and B viruses and parainfluenza type 3 virus up to the concentration of treatment of cells with SP2 did not protect the cells from RSV infection. This polysaccharide addition assay showed that when SP2 was present only during virus adsorption phase at 37℃, it could significantly inhibit RSV-induced plaque formation. Conclusions SP2 might be a potent substance for treating RSV infection. The mode of action of SP2 might depend on inhibiting RSV attachment to host cells.

Objective To search for triterpene glycoside with new structure. Methods We studied the sea cucumber Colochirous anceps belonging to the family cucumariidea with many chromatography methods and found a new sulfated triterpene glycoside, which structure was established by a combination of spectroscopic analysis (IR, UV, ESI-MS and 2D-NMR), chemical transformations and other chemical evidence. Results and Conclusion The new sulfated triterpene glycoside was isolated and named Colochiroside A (1) .

Objective To investigate the influences of Rhodospirillaceae on immune function of mice.Methods Four tests were performed in mice after 4 w medication with different doses of Rhodospirillaceae ig,including:1.The transformation of spleen lymphocyte(MTT method);2.The phagocytosis of macrophagocyte(semi-vivo method);3.The value of HC50; 4.The activity of NK cell(LDH method).Results1.The transformation of spleen lymphocyte;2.The phagocytosis of macrophagocyte,the Value of HC50 and the activity of NK cell in test groups were all raised,the differences were significant and very significant when compared with the control group.Couclusions Rhodospirillaceae could enhance the cellular and humoral immunity of mice.

Objective To study the extraction and purification of polysaccharides from D.salina residue after extraction of ?-carotene.Methods The crude polysaccharide was extracted by hot basic water,and then was isolated and purified by the DEAE-cellulose and Sepharose-4B.Results After having been purified by DEAE-cellulose chromatography,three components named PDS_(1),PDS_(2),PDS_(3)were obtained.Each component was divided into two components by Sepharose-4B chromatography,then six components were obtained.Conclusion The six components were identified to be homogeneous polysaccharide-protein complexes which were composed of sulfate radical and uronic acid.

Objective To search for novel antifungal structures produced by marine fungus.Methods Using the Pyricularia oryzae (P.oryzaae) bioassay method to screen marine fungi led to the isolation of marine fungus 09-1-1-1.Active metabolites were isolated from the broth of the marine fungus 09-1-1-1.Results Two compounds were extracted from the mycelium and their structures were elucidated by various spectroscopic methods as 3a,12c-dihydro-8-hydroxy-6-methoxy-7H-furo\furo\xanthen-7-one(Ⅰ,sterigmatocystin) and 1,3,6,8-tetrahydroxy-2-(1-hydroxyhexyl)anthraquinone(Ⅱ,averantin).Conclusion The P.oryzae bioassay was a cheap,quick and convenient method for the screening of bioactive substances from marine fungi.Compound Ⅱ showed potent inhibitory activity to the mycelia of P.oryzae.The MIC of compound Ⅱagainst P.oryzae was 1.6 ?g?mL~(-1).

Objective To study the single and combined-enzymic hydrolysis conditions of Oreochromis niloticus skin collagen.Methods Based on the inhibitory activities of six kinds of single enzymic hydrolysates to angiotensin converting enzyme(ACE),bromelain and alcalase(2.4L) were selected as combined-enzyme.The optimal hydrolytic conditions of the skin collagen with the two enzymes were determined by orthogonal test L_(16)(4~(5)).Results and Conclusion The results showed that the optimal hydrolytic conditions of bromelain and alcalase 2.4L were temperature 45℃,pH4.5,E/S 4000U?g~(-1),time 4h,concentration of substrate 6%;and temperature 55℃,pH7.5,E/S 6000U?g~(-1),time 2h,concentration of substrate 4%,respectively.Furthermore,hydrolytic effect would be better when using bromelain in advance,followed by alcalase 2.4L.The hydrolytic degree of the hydrolysates prepared under the provided technology was 30.43%,the ACE inhibitory rate would be as high as(68.65%) in vitro.

Objective To find new compound from soft corals.Methods The chloroform portion of the soft coral Sinularia sp.was subjected to silica gel to give ceramide(Ⅰ).Results Structure of compound(Ⅰ) was identified as 2S,3S,4R-N-octadecanoyl-phytosphingosine on the bases of spectroscopic(IR,MS,NMR) and chemical analyses.Conclusion Compound(Ⅰ) was a new natural ceramide and its IR, NMR data were reported here for the first time.

By making investigation on the animals with venom protein and peptied.In China sea we find that Coelenterata of them mainly includes 14 kinds of Jellyfist,15 kinds of Sea anemone,one kind of Corals and two kinds of Hydrozoa,altogether 32 kinds;Mollusea of them mainly includes 34 kinds of Conus,two kinds of Aplysia and 3 kinds of Cephalopoda,altogether 39 kinds;Nemartina of them has one kind;Echinodermata of them mainly includes two kinds of starfish and 8 kinds of Sea urchins,altogether 10 kinds;fishes of them mainly include one kind of Venomous fishes,two kinds of Ichthyocrinotoxic fishes and 74 kinds of Venomous fishes,altogether 77 kinds;Sea snake is 15 kinds.These animals with venom protein and peptide mainly are distributed in South sea of China.