Objective To investigate the expression and clinical diagnosis significance of serum long non-coding RNA PANDAR(lncRNA PANDAR)and procollagen type I C-terminal propeptide(PICP)in diabetic nephropathy(DN).Methods A total of 310 patients with type 2 diabetes mellitus(T2DM)admitted to the Third Affiliated Hospital of Chengdu Medical College from January 2022 to December 2023 were selected as the case group,and further divided into DM group(n=190)and DN group(n=120)based on the presence or absence of concomitant kidney disease.60 healthy subjects were selected as the control group.The expression level of lncRNA PANDAR was determined by real time fluorescence quantitative PCR,and the serum PICP level was determined by enzyme-linked immunosorbent assay(ELISA).The expression of lncRNA PANDAR and serum PICP in each group was compared.Logistic regression was used to analyze the influencing factors of DN.ROC curve was used to evaluate the diagnostic value of serum lncRNA PANDAR,PICP and their combination in DN.Results The levels of serum lncRNA PANDAR(4.56±1.26),PICP(147.72±19.20 μg/L)in the case group were higher than those in the control group(2.27±0.61,117.24±12.72 μg/L),and the differences were statistically significant(t=13.758,11.799,all P<0.05).With the increase of chronic kidney disease(CKD)stage,the levels of serum lncRNA PANDAR and PICP gradually increased,and the differences were statistically significant(F=16.790,286.87,all P<0.05).The levels of serum lncRNA PANDA(5.27±1.26)and PICP(159.59±17.10 μg/L)in DN group were higher than those in DM group(4.11±1.03,140.22±16.56 μg/L),the differences were statistically significant(t=-8.847,-9.905,all P<0.05).Multivariate Logistic regression analysis showed that lncRNA PANDA and PICP were independent risk factors for DN in T2DM patients(Wald χ2=11.592,7.880,all P<0.05).The areas under curve(AUC)of serum lncRNA PANDA and PICP combined detection of DN in patients with T2 DM was higher than that of single index detection,and the differences were statistically significant(Z=-2.006,-3.069,all P<0.05).Conclusion The levels of serum lncRNA PANDAR and PICP in patients with DN are increased,and their combined detection can improve the diagnostic efficiency of DN.

Objective To investigate the expression and clinical diagnosis significance of serum long non-coding RNA PANDAR(lncRNA PANDAR)and procollagen type I C-terminal propeptide(PICP)in diabetic nephropathy(DN).Methods A total of 310 patients with type 2 diabetes mellitus(T2DM)admitted to the Third Affiliated Hospital of Chengdu Medical College from January 2022 to December 2023 were selected as the case group,and further divided into DM group(n=190)and DN group(n=120)based on the presence or absence of concomitant kidney disease.60 healthy subjects were selected as the control group.The expression level of lncRNA PANDAR was determined by real time fluorescence quantitative PCR,and the serum PICP level was determined by enzyme-linked immunosorbent assay(ELISA).The expression of lncRNA PANDAR and serum PICP in each group was compared.Logistic regression was used to analyze the influencing factors of DN.ROC curve was used to evaluate the diagnostic value of serum lncRNA PANDAR,PICP and their combination in DN.Results The levels of serum lncRNA PANDAR(4.56±1.26),PICP(147.72±19.20 μg/L)in the case group were higher than those in the control group(2.27±0.61,117.24±12.72 μg/L),and the differences were statistically significant(t=13.758,11.799,all P<0.05).With the increase of chronic kidney disease(CKD)stage,the levels of serum lncRNA PANDAR and PICP gradually increased,and the differences were statistically significant(F=16.790,286.87,all P<0.05).The levels of serum lncRNA PANDA(5.27±1.26)and PICP(159.59±17.10 μg/L)in DN group were higher than those in DM group(4.11±1.03,140.22±16.56 μg/L),the differences were statistically significant(t=-8.847,-9.905,all P<0.05).Multivariate Logistic regression analysis showed that lncRNA PANDA and PICP were independent risk factors for DN in T2DM patients(Wald χ2=11.592,7.880,all P<0.05).The areas under curve(AUC)of serum lncRNA PANDA and PICP combined detection of DN in patients with T2 DM was higher than that of single index detection,and the differences were statistically significant(Z=-2.006,-3.069,all P<0.05).Conclusion The levels of serum lncRNA PANDAR and PICP in patients with DN are increased,and their combined detection can improve the diagnostic efficiency of DN.

Objective To investigate the risk factors for pediatric acute perforation appendicitis,and to construct a nomogram predictive model and conduct the verification.Methods A total of 426 children patients with appendectomy in this hospital from June 30,2020 to June 30,2022 were selected as the study subjects 340 children with acute appendicitis admitted to the hospital from 30 June 2020 to 28 February 2022 were the training set and 86 children patients with appendicitis hospitalized in this hospital from March 1,2022 to June 30,2022 conducted the external validation(verification set).The univariate and multivariate logistic regression models were employed to analyze the independent risk factors of pediatric acute perforation appendicitis.The nomograms predictive model was constructed.The receiver operating characteristic(ROC)curve and calibra-tion curve were used to evaluate the predictive efficiency of the model.The decision curve analysis(DCA)was used to evaluate the application value of the model.Results Of the 426 children,198 were perforated and 228 were not perforated.The univariate and multivariate logistic regression analyses revealed that elevated C-reac-tive protein(CRP),presence of stercorolith in appendiceal cavity,time of onset to visiting hospital ≥2 d and body temperature ≥37.3 ℃ were the independent risk factors for pediatric acute perforation appendicitis(P＜0.05).The Hosmer-Lemeshow test demonstrated that the nomogram predictive model had good fitting(P=0.869),and the area under the curve(AUC)for the training and validation sets were 0.808 and 0.860 respectively,showing the good predictive ability of the model.The calibration curve closely approach the ideal diagonal.The model showed good discrimination,consistency and accuracy.The DC A revealed that the curve was far away from oblique and horizontal lines,and the model had good clinical practicability.Conclusion The constructed nomogram model of pediatric acute perforation appendicitis has good predictive ability and may help clinic to identify as early as possible.

PI3K/AKT/mTOR signaling pathway plays a crucial role in cell proliferation, survival, differentiation, motility and intracellular transport. PI3Kδ, an isoform of PI3K, is highly expressed in B lymphocytes and is involved in the malignant progression of B-cell lymphoma. Thus, PI3Kδ has emerged as an attractive target for the development of anti-B-cell lymphoma drugs. Currently, there are several PI3Kδ inhibitors approved by the FDA for the treatment of B-cell lymphoma, each with its own characteristics, but also with varying degrees of adverse effects in clinical practice. Due to the complexity and diversity of the pathogenesis of B-cell lymphoma, single-target PI3Kδ inhibitors often have limited efficacy and are prone to drug resistance, and need to be combined with chemotherapy or other targeted drugs to enhance their efficacy. The future trend is to design novel inhibitors with higher efficacy and lower toxicity or to develop novel combination regimens with other chemotherapy, radiotherapy, and target drugs to acquire better anti-tumor effects with reduced adverse effects. This review summarizes the PI3Kδ inhibitors that have been approved for the treatment of B-cell lymphoma or are still in clinical trials, mainly focusing on their characteristics, adverse effects and combination regimens.

Objective: To summarize the diagnosis and prognosis of adrenocortical carcinoma in children. Methods: Medical records of 10 pediatric patients with adrenocortical cancer treated in Children's Hospital of Nanjing Medical University from September 2010 to March 2018 were retrospectively reviewed, and their clinical manifestation, laboratory, auxiliary, and pathological examination results, and prognosis were analyzed. Results: The average age of all patients (5 male and 5 female pediatric patients) was (53.3+48.4) months. Clinical mani-festations included abdominal mass, hypertension, cortisol level, and peripheral precocious puberty. Laboratory tests showed that lactate dehydrogenase, neuroenolase, and cortisol levels were high in some patients, and sex hormones were abnormal. Auxiliary examination using the abdominal computed tomography enhancement showed irregular an adrenal gland, uneven density, necrosis, mass calcification, and some infiltration into the surrounding tissue. Pathological examination suggests tumor cell pleomorphism, more frequent pathological division, high mitotic index, abundant interstitial sinusoid, necrosis, tumor infiltration, vascular invasion, and Weiss score of≥3 points. Lactate acid dehydrogenase and cortisol levels; Weiss score; and tumor size, staging, and resection rate were not statistically different among prognostic factors. Conclusions: For pediatric patients with adrenal masses, the survival rate should be improved by combining the clinical manifestation, laboratory examination, and auxiliary examination for the early diagnosis of adrenocortical carcinoma and to select the appropriate treatment. Combined with pathological results, adrenocortical carcinoma can be well differentiated from other adrenal masses.

Objective To analyze the prognostic factors of adrenal tumor in children under 12 years of age. Methods A total of 90 children with 97 adrenal tumors admitted from June 2006 to August 2017 were selected in Children's Hospital of Nanjing Medical University.The age distribution,tumor type,biochemistry and tumor indicators, treatment,stage classification and prognosis were analyzed.Results There were 46 males and 44 females in 90 cases. Ages ranged from 4 days to 11 years and 1 month,with an average of (38.1 ± 31.3)months.The main clinical mani-festations were abdominal mass,fever and abdominal pain.Eighty cases (82.5%)underwent surgery,while 17 cases (17. 5%)did not.Open resection was performed in 48 cases,open partial resection in 11 cases,laparoscopic surgery in 10 cases,and just biopsy in 11 cases.The pathological examination showed 43 cases with neuroblastoma,13 cases with ganglioneuroblastoma,8 cases with ganglioneuroma,5 cases with adrenocortical carcinoma,3 cases with teratoma,1 case with pheochromocytoma,1 case with malignant rhabdoid tumor.Statistical analysis revealed that neuron－specific eno-lase(NSE)value of neuroblastoma and lactate dehydrogenase(LDH)value of cortical cancer increased significantly. The age was correlated with tumor stage,and patients had older age on stage Ⅳ.Complete resection in surgery was correlated with the stage of the tumor,as tumor in lower tumor stage seemed easier to be completely removed.Fifty－three cases (58.9%)were followed up for 2 months up to 11 years and 4 months.Forty－four cases survived and 9 ca-ses died.Higher tumor stage predicated worse prognosis.Conclusions Adrenal gland tumors need early diagnosis and active treatment.Earlier onset of age,complete surgical resection with patients have better prognosis.Complete resection of the disease was a key factor in the prognosis.

Objective To compare the clinical effects of botulinum toxin injection and radiofre quency thermal rhizotom in the treatment of primary hemifacial spasm.Methods A total 118 primary hemifacial spasm patients were randomly divided into two groups,who were received botulinum toxinA injections and radiofrequency thermal rhizotom treatment,respectively.Patients were followed up,and the curative effect,complications and recurrence rates of the two treatments were statistically compared.Results The early curative effect (1 month) for primary hemifacial spasm in botulinum toxin injection group was better than that in radiofrequency thermal rhizotom treatment group:recurrence rate of long-term (6 months) was 72.5 ％,but caused a very low risk of facial paralysis; radiofrequency thermal rhizotom treatment had a long-term effect as compared with botulinum toxin injection,6 months recurrence rate was 21.4 ％,with a low rate of postoperative facial paralysis.Conclusions Botulinum toxin injections is higher in short-term effectiveness and smaller in side effects as compared with the treatment of radiofrequency thermal rhizotom. However,radiofrequency thermal rhizotom treatment is more stable in the prospective efficacy.