Objective: To explore the early effects of birth weight at different gestational ages on blood pressure trajectory among primary school students, so as to provide evidence for incorporating gestational age birth weight into individualized early warning and intervention strategies for childhood hypertension. Methods: From May to November 2023, a purposeful sampling method was used to recruit 1 676 students in grade 1-3 from three primary schools in a certain urban district of Chongqing. Follow up assessments were conducted in May 2024(T1), November 2024(T2), and May 2025(T3). General demographic and birth related information were collected via self administered questionnaires, while height, weight and blood pressure were obtained through physical examinations. Linear mixed effects model was used to analyze the associations between birth weight at different gestational ages and blood pressure trajectories. Results: During the T1-T3 period, the systolic blood pressure of boys were 98.5 (93.0, 104.5 ),98.5 (93.5, 105.0), and 97.5 (92.5, 103.5)mmHg, respectively, while the diastolic blood pressure were 60.5 (56.5, 65.0), 61.5 ( 57.0 , 65.5), and 60.0 (56.0, 64.0)mmHg, respectively. For girls, the systolic blood pressure were 95.5 (90.0, 102.0),95.5 (90.5, 101.5), and 96.0 (90.5, 101.5)mmHg, respectively, and the diastolic blood pressure were 60.5 (56.0, 64.5 ),61.5 (57.5, 65.5), and 59.5 (56.0, 63.0)mmHg, respectively. Through Friedman test within both boys and girls, diostolic blood pressure were statistically significant across three measurements( χ 2=48.85，81.54，both P <0.01). The proportion of normal blood pressure increased , and the proportion of prehypertension and hypertension decreased with time( χ 2=39.72，25.62，both P < 0.01 ). Linear mixed effects model analysis revealed that after adjusting for age, sex, household income monthly, parental education, family history of hypertension and maternal pregnancy complications, large for gestational age had significantly higher trajectories of systolic ( β = 1.50) and diastolic( β =0.94) blood pressure compared to appropriate for gestational age(both P <0.01). Conclusion Large for gestational age is associated with elevated blood pressure trajectories during school age, and it may be considered as an early indicator for individualized screening and intervention for childhood hypertension.

Objective To analyze the application effects of artificial intelligence (AI) software and Mimics software in preoperative three-dimensional (3D) reconstruction for thoracoscopic anatomical pulmonary segmentectomy. Methods A retrospective analysis was conducted on patients who underwent thoracoscopic pulmonary segmentectomy at the Second People's Hospital of Huai'an from October 2019 to March 2024. Patients who underwent AI 3D reconstruction were included in the AI group, those who underwent Mimics 3D reconstruction were included in the Mimics group, and those who did not undergo 3D reconstruction were included in the control group. Perioperative related indicators of each group were compared. Results A total of 168 patients were included, including 73 males and 95 females, aged 25-81 (61.61±10.55) years. There were 79 patients in the AI group, 53 patients in the Mimics group, and 36 patients in the control group. There were no statistical differences in gender, age, smoking history, nodule size, number of lymph node dissection groups, postoperative pathological results, or postoperative complications among the three groups (P>0.05). There were statistical differences in operation time (P<0.001), extubation time (P<0.001), drainage volume (P<0.001), bleeding volume (P<0.001), and postoperative hospital stay (P=0.001) among the three groups. There were no statistical differences in operation time, extubation time, bleeding volume, or postoperative hospital stay between the AI group and the Mimics group (P>0.05). There was no statistical difference in drainage volume between the AI group and the control group (P=0.494), while there were statistical differences in operation time, drainage tube retention time, bleeding volume, and postoperative hospital stay (P<0.05). Conclusion For patients requiring thoracoscopic anatomical pulmonary segmentectomy, preoperative 3D reconstruction and preoperative planning based on 3D images can shorten the operation time, postoperative extubation time and hospital stay, and reduce intraoperative bleeding and postoperative drainage volume compared with reading CT images only. The use of AI software for 3D reconstruction is not inferior to Mimics manual 3D reconstruction in terms of surgical guidance and postoperative recovery, which can reduce the workload of clinicians and is worth promoting.

In continuation of research aimed at identifying anti-inflammatory agents from natural sesquiterpenoids, an activity-guided fractionation approach utilizing lipopolysaccharide (LPS)-mediated RAW264.7 cells was employed to investigate chemical constituents from Inula Britannica (I. britannica). Seven novel sesquiterpenoid dimers inulabritanoids A-G (1-7) and two novel sesquiterpenoid monomers inulabritanoids H (8) and I (9) were isolated from I. britannica together with eighteen known compounds (10-27). The structural elucidation was accomplished through comprehensive analysis of 1D and 2D nuclear magnetic resonance (NMR), high-resolution mass spectrometry (HR-MS), and electronic circular dichroism (ECD) spectra, complemented by quantum chemical calculations. Compounds 1, 2, 12, 16, 19, and 26 demonstrated inhibitory effects on NO production, with IC₅₀ values of 3.65, 5.48, 3.29, 6.91, 3.12, and 5.67 μmol·L^-1, respectively. Mechanistic studies revealed that compound 1 inhibited IκB kinase β (IKKβ) phosphorylation, thereby blocking nuclear factor κB (NF-κB) nuclear translocation, and activated the kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) signal pathway, leading to decreased expression of NADPH oxidase 2 (NOX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor α (TNF-α), interleukin-6 (IL-6), monocyte chemotactic protein-1 (MCP-1), IL-1β, and IL-1α and increased expression of NAD(P)H: quinone oxidoreductase 1 (NQO-1) and heme oxygenase-1 (HO-1), thus exhibiting anti-inflammatory effects in vitro. These results indicate that dimeric sesquiterpenoids may serve as promising candidates for anti-inflammatory drug development.
Mice ; Animals ; Sesquiterpenes/isolation & purification* ; Anti-Inflammatory Agents/isolation & purification* ; Inula/chemistry* ; RAW 264.7 Cells ; Nitric Oxide ; Molecular Structure ; NF-kappa B/immunology* ; NF-E2-Related Factor 2/immunology* ; Macrophages/immunology* ; Nitric Oxide Synthase Type II/immunology* ; Plant Extracts/pharmacology* ; Lipopolysaccharides ; Tumor Necrosis Factor-alpha/immunology* ; I-kappa B Kinase/genetics*

Objective To study the expression,immunological effect and prognosis analysis of G2 and S phase-expressed protein 1(GTSE1)in hepatocellular carcinoma(HCC),and its potential action mechanism.Methods Using the data provided by the public da-tabases The Cancer Genome Atlas(TCGA),the Kaplan-Meier,Tumor Immune Estimation Resource(TIMER)and Gene Expression Profiling Interactive Analysis(GEPIA)databases were used to analyze the gene expression,immunological effect and prognosis of GTSE1,and the expression of GTSE1 in clinical samples was verified by immunohistochemical experiments.Gene Ontology(GO)and Kyoto Ency-clopedia of Genes and Genomes(KEGG)enrichment analysis of GTSE1-related differential genes was performed by R software.Results GTSE1 was significantly overexpressed in human cancer tissues,and was significantly associated with poor prognosis of liver cancer(P＜0.05).GTSE1 gene expression was significantly correlated with the abundance of infiltrating immune cells in HCC(P＜0.001).GTSE1-related differentially expressed genes were mainly enriched in gene modules such as nuclear division,organelle fission and ion channel activity.The signaling pathways involved mainly include neuroactive ligand-receptor interaction and cell cycle.Conclusion GTSE1 expression is significantly up-regulated in HCC and is significantly associated with poor prognosis,and plays an important role in immune cell infiltration,which can be used as a prognostic marker and immunotherapeutic target for HCC.

Objective:To investigate the effects of breast milk to total milk intake ratio during hospitalization on the duration of antibiotic therapy in preterm infants less than 34 weeks of gestation.Methods:Clinical data of preterm infants ( n=1 792) less than 34 gestational weeks were retrospectively collected in 16 hospitals of Jiangsu Province Neonatal-Perinatal Cooperation Network from January 1, 2019, to December 31, 2021. The days of therapy (DOT) were used to evaluate the duration of antibiotic administration. The median DOT was 15.0 d (7.0-27.0 d). The patients were divided into four groups based on the quartiles of DOT: Q 1 (DOT≤7.0 d), Q 2 (7.0 d27.0 d) groups. According to the breast milk intake ratio (breast milk intake to total milk intake during hospitalization×100%), they were also divided into four groups: very-low-ratio breastfeeding group (breast milk intake ratio≤25%), low-ratio breastfeeding group (25%75%). Univariate analysis ( Chi-square test and Kruskal-Wallis rank-sum test) was used to analyze the factors influencing DOT. Spearman correlation analysis and trend Chi-square test were used to explore the relationship between breast milk intake ratio and DOT. After using multiple imputations to address missing data, two models were constructed after adjusting for different factors, and multinomial logistic regression model was applied to evaluate the effects of the breast milk intake ratio on DOT. Finally, sensitivity analysis was conducted to assess the stability of the models. Results:(1) Of the 1 792 preterm infants, there were 507 (28.3%) in the Q 1 group, 422 (23.5%) in the Q 2 group, 438 (24.4%) in the Q 3 group and 425 (23.7%) in the Q 4 group. (2) The median values of DOT in the very-low-ratio, low-ratio, medium-ratio and high-ratio breastfeeding groups were 20.0 d (11.0-31.0 d), 20.0 d (11.0-32.0 d), 13.0 d (6.0-25.8 d) and 10.0 d (4.0-21.0 d), respectively. Compared with the very-low-ratio and low-ratio breastfeeding groups, the medium-ratio and high-ratio breastfeeding groups had shorter DOT (all P<0.05). (3) After adjusting for factors with P<0.1 (prenatal glucocorticoid exposure, antimicrobial use within 24 h before delivery, gestational age at delivery, birth weight, Apgar score≤7 at 1 min, neonatal respiratory distress syndrome, infectious pneumonia and early-onset neonatal sepsis) between the DOT quartile groups, it showed that medium-ratio and high-ratio breastfeeding were protective factors in contrast to very-low-ratio breastfeeding in the Q 2, Q 3 and Q 4 groups as compared with the Q 1 group [Q 2 group: OR=0.50 (95% CI: 0.30-0.85) and OR=0.36 (95% CI: 0.26-0.51); Q 3 group: OR=0.31 (95% CI: 0.18-0.55) and OR=0.20 (95% CI: 0.14-0.29); Q 4 group: OR=0.22 (95% CI: 0.12-0.42) and OR=0.17 (95% CI: 0.12-0.26)]. Conclusion:Breast milk intake accounting for over 50% of total milk intake has a positive impact on reducing DOT in premature infants requiring antibiotics, which suggests that breastfeeding should be actively encouraged.

Objective:To construct a prediction model of pancreatic fistula after pancreaticoduodenectomy and explore its application value.Methods:The retrospective case-control study was conducted. The clinicopathological data of 285 patients with periampullary diseases who underwent pancreaticoduodenectomy in the the First Affiliated Hospital of Harbin Medical University from January 2015 to September 2018 were collected. There were 183 males and 102 females, aged (56±14)years, with a range from 12 to 84 years. According to the random numbers showed in the computer, patients were randomly divided into training dataset consisting of 214 patients and validation dataset consisting of 71 patients, with a ratio of 3∶1. The training dataset was used to construct prediction model, and the validation dataset was used to evaluate performance of prediction model. Observation indicators: (1) incidence of postoperative pancreatic fistula; (2) construction of prediction model of pancreatic fistula after pancreaticoduodenectomy; (3) validation of prediction model of pancreatic fistula after pancreaticoduodenectomy. Measurement data with normal distribution were represented as Mean± SD, and comparison between groups was analyzed by the t test. Measurement data with skewed distribution were represented as M (range), and comparison between groups was analyzed using the Mann-Whitney rank sum test. Count data were described as absolute numbers or percentages, and comparison between groups was conducted using the chi-square test. Univariate analysis and multivariate analysis were conducted using the Logistic regression model. The accuracy of prediction model was analyzed by drawing receiver operating characteristic curve and calculating area under curve (AUC). Results:(1) Incidence of postoperative pancreatic fistula: of 214 patients in the training dataset, 45 patients had postoperative pancreatic fistula, including 39 of grade B and 6 of grade C, respectively. (2) Construction of prediction model of pancreatic fistula after pancreaticoduodenectomy. Results of univariate analysis showed that body mass index(BMI), diameter of the main pancreatic duct on computed tomography (CT) scan, diameter of the main pancreatic duct by intraoperative exploration, pancreas texture, and level of amylase in ascites at the postoperative first day were related factors for pancreatic fistula after pancreaticoduodenectomy ( χ2=32.450, 15.789, 19.577, 4.559, Z=-7.962, P<0.05). Results of multivariate analysis showed that BMI>25 kg/m 2, diameter of the main pancreatic duct by intraoperative exploration <3 mm and level of amylase in ascites at the postoperative first day >2 651U/L were independent risk factors for pancreatic fistula after pancreaticoduodenectomy ( odds ratio=0.148, 4.286, 0.086, 95% confidence interval: 0.058-0.376, 1.736-10.580, 0.032-0.231, P<0.05). Based on results of multivariate analysis, a prediction model of pancreatic fistula after pancreaticoduodenectomy was built: the predicted value of pancreatic fistula=Exp[0.452-1.914(BMI)+ 1.455(diameter of the main pancreatic duct by intraoperative exploration)-2.451(level of amylase in ascites at the postoperative first day)]/1+ Exp[0.452-1.914(BMI)+ 1.455(diameter of the main pancreatic duct by intraoperative exploration)-2.451(level of amylase in ascites at the postoperative first day)]. The model had the AUC of 0.888 (95% confidence interval : 0.832-0.943, P<0.05). (3) Validation of prediction model of pancreatic fistula after pancreaticoduodenectomy: in the validation dataset, the prediction model of pancreatic fistula after pancreaticoduodenectomy had the AUC of 0.868 (95% confidence interval: 0.780-0.957, P<0.05). There was no significant difference in the AUC between the training dataset and validation dataset ( Z=0.514, P>0.05). Conclusions:BMI>25 kg/m 2, diameter of the main pancreatic duct by intraoperative exploration <3 mm and level of amylase in ascites at the postoperative first day >2 651 U/L are independent risk factors for pancreatic fistula after pancreaticoduodenectomy. Construction of a prediction model of pancreatic fistula after pancreaticoduo-denectomy can effectively predict the risks of postoperative pancreatic fistula.

Objective:To investigate the feasibility and safety of right-sided abdominal evisceration in retroperitoneal liposarcoma.Methods:The clinical data of 16 cases of retroperitoneal liposarcoma performed with right-sided abdominal evisceration at Sarcoma Center of Peking University Cancer Hospital from Sep 2015 to Feb 2019 were analyzed retrospectively.Results:Complete resection were successfully performed in all 16 cases.The median tumor size was 29cm(13-43 cm), the median operative time was 660 min(429-940 min), the median estimated blood loss was 2 000 ml(300-6 000 ml). The major postoperative complications rate (Clavien-Dindo classification Ⅲ-Ⅴ) was 38%. Median overall survival is 41.0 months while the median disease-free survival is 32.6 months.Conclusions:Right-sided abdominal evisceration is a favorable procedure to attain complete resection with acceptable complication rate.

Objective:To explore the application value of CT measurement of renal depth correction, optimized acquisition and post-processing in the measurement of renal glomerular filtration rate (GFR) by Gates renal dynamic imaging.Methods:From January 2018 to November 2019, 157 patients (102 males, 55 females, age (51.4±14.5) years) including 118 in normal renal area group (adults with normal renal position and morphology, and excluding hydronephrosis, renal occupation, retroperitoneal mass and other factors affecting renal depth) and 39 in abnormal renal area group (19 of transplanted kidney, 11 of horseshoe kidney and 9 of ectopic kidney), were retrospectively enrolled in Union Hospital, Tongji Medical College, Huazhong University of Science and Technology. The GFR was measured by renal dynamic imaging Gates method. For the normal renal area group, the renal depth was calculated by CT method, the traditional Tonnesen formula or the Li Qian formula. For the abnormal renal area group, the GFR was measured by optimized acquisition and post-processing method (GFR optimization), the traditional post-processing method (GFR tradition), or Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula method (eGFR). The differences of the renal depth and corresponding GFR obtained by different methods were analyzed using one-way analysis of variance and the least significant difference (LSD) t test. The correlation was analyzed by Pearson correlation analysis, and the consistency was analyzed by Bland-Altman analysis. Results:In the normal renal area group, the left and right renal depth measured by CT were (7.40±1.43) and (7.51±1.37) cm. Tonnesen formula underestimated renal depth (left kidney: (6.03±0.82) cm, right kidney: (6.06±0.84) cm; F values: 64.145 and 68.567, both P<0.01), and the deviation increased with the increase of CT measured depth ( r values: 0.847 and 0.834, both P<0.01). The GFR measured by Tonnesen formula was (56.93±28.42) ml·min -1·1.73 m -2, and the difference was statistically significant compared with CT method ((73.43±36.56) ml·min -1·1.73 m -2; F=9.423, P<0.01). The renal left and right depth measured by Li Qian formula were (7.55±1.03) and (7.52±0.98) cm, and the total GFR was (73.65±34.50) ml·min -1·1.73 m -2 with no differences compared with CT method (all P>0.05). The GFR obtained by Li Qian formula had better correlation ( r=0.901, P<0.01) and consistency with CT method. In the abnormal renal area group, GFR optimization, GFR tradition and eGFR was (63.11±27.40), (48.40±25.45) and (59.89±32.24) ml·min -1·1.73 m -2, respectively, and the difference between GFR tradition and GFR optimization was statistically significant ( F=2.870, P=0.025). GFR optimization had better correlation ( r=0.941, P<0.01) and consistency with eGFR. Conclusions:Tonnesen formula underestimates the renal depth. Using CT to measure renal depth and perform depth correction can improve the accuracy of Gates method for GFR determination. For the special cases of transplanted kidney, horseshoe kidney, ectopic kidney and retroperitoneal mass, it is important to optimize acquisition scheme and post-processing method to obtain accurate GFR.

Objective:To evaluate the effects of different sphere volumes, target background ratio (T/B) and post-processing correction techniques on the quantitative results of 99Tc m SPECT/CT. Methods:Six spheres with different diameters (37, 28, 22, 17, 13, 10 mm) in National Electrical Manufacturers Association International Electrotechnical Commission (NEMA IEC) models were filled with a mixture of 0.54 MBq/ml 99Tc m and iodixanol. The mixture iodine content was about 0.3%(135 mg), which led to different T/B (32∶1, 16∶1, 8∶1, 4∶1) by changing the radioactivity concentration of the cylinder. Routine imaging was performed on different T/B phantoms which were scanned by SPECT/CT. The CT threshold method was used for the delineation of volume of interest (VOI). Then the same processing correction technique and ordered-subsets expectation maximization (OSEM) parameters were used to calculate the radioactivity concentrations of different spheres, and further compared with the true values, and the accuracies were calculated. Pearson correlation analysis was applied to evaluate the relationships between sphere volume, T/B and quantitative results. The sphere with T/B of 32∶1 and diameter of 37 mm were processed by 3 correction techniques (CT attenuation correction (CTAC)+ scatter correction (SC)+ resolution recovery (RR); CTAC+ SC; CTAC+ RR). One-way analysis of variance and the least significant difference t test were used to analyzed the effects of 3 correction techniques on the quantitative results and image contrasts. Results:There were significant relationships between the sphere volumes, T/B and the quantitative accuracy ( r values: 0.757, 0.409, both P<0.05). There were significant differences of 3 correction techniques on the quantitative results and image contrast ( F values: 139.665 and 38.905, both P<0.001). Among them, the quantitative error of CTAC+ SC+ RR was lower than that of CTAC+ SC ((9.63±8.82)% vs (38.89±2.17)%; P<0.001), and similar to that of CTAC+ RR ((8.70±6.64)%; P>0.05). The quantitative error of CTAC+ RR was lower than that of CTAC+ SC ( P<0.001). The image contrast of CTAC+ SC+ RR was higher than that of CTAC+ SC ((93.45±0.91)% vs (92.41±0.25)%; P<0.001) and the image contrast of CTAC+ SC was higher than that of CTAC+ RR ((91.37±0.87)%; P<0.001). Conclusions:The larger sphere volume and the higher T/B, the more quantitative accuracy. The volume has a more significant effect on quantitative accuracy than T/B. Choosing the appropriate correction technique is helpful to quantitative accuracy improvement. It is suggested to use CTAC+ SC+ RR in quantitative processing.

Objective To investigate the difference of Theory of Mind (ToM) processing (especially in social-perceptual component and social-cognitive component) in male methamphetamine (METH) addicts, and test whether the deficits of ToM is correlated with their psychotic symptoms or not. Methods Thirty METH addicts with psychotic symptoms (METH-P group), 31 METH addicts with no psychotic symptoms (METH-NP group) and 41 healthy controls (control group) were recruited. Eyes Task and Faux pas Task were used to test the social-perceptual component and social-cognitive component in all participants. The psychotic symptoms of METH addicts were assessed using Brief Psychiatric Rating Scale (BPRS). Results Compared with control group, performance was poor in Eyes Task and Faux pas Questions in both METH-P group and METH-NP group (P＜0.05). Performance in Eyes Task was poorer in METH-P group than in METH-NP group (P＜0.05). There were no significant differences in performance in Faux pas Questions scores between METH-P group and METH-NP group (P＞0.05). Multivariate linear regression analysis demonstrated that BPRS positive symptom score were correlated with Eyes Task scores of METH addicts (β=-0.415, P=0.001). Conclusions METH addicts exhibit deficits in both ToM social-perceptual component and ToM social-cognitive component. METH-associated psychosis are related to the deficit in social-perceptual component of ToM.