Objective:To investigate the effect of polypyrimidine tract binding protein 1 (PTBP1) on the proliferation and apoptosis of osteosarcoma cell lines via the phosphatase and tensin homolog (PTEN)-protein kinase B (Akt) signaling pathway.Methods:Osteosarcoma cell lines MG63, U2-OS, Saos-2, 143B, and the osteoblast cell line hFOB1.19 were selected. Western blotting was used to detect the relative expression level of PTBP1 protein, and the Saos-2 cell line with the highest PTBP1 relative expression level was selected for subsequent experiments. Saos-2 cells were divided into 5 groups: si-NC group [transfected with negative control siRNA (NC siRNA)], si-PTBP1 group (transfected with PTBP1 siRNA), solvent control+si-NC group [treated with dimethyl sulfoxide(DMSO) to a final concentration of 0.1% and transfected with NC siRNA], solvent control+si-PTBP1 group (treated with 0.1% DMSO and transfected with PTBP1 siRNA), and SF1670+si-PTBP1 group (treated with 10 μmol/L SF1670, a PTEN inhibitor, and transfected with PTBP1 siRNA). CCK-8 assay was used to detect the cell proliferation [expressed as the absorbance value at 490 nm ( A490)]; TUNEL staining was used to detect apoptosis ability, and apoptosis rate was calculated; Western blotting was used to detect the expression levels of relevant proteins. Results:The relative expression level of PTBP1 protein in osteosarcoma MG63, U2-OS, Saos-2, 143B, and osteoblast cell line hFOB1.19 cells was 0.80±0.12, 0.55±0.08, 1.04±0.10, 0.49±0.07, and 0.26±0.05, respectively, and the difference was statistically significant ( F = 47.10, P < 0.001). CCK-8 assay result showed that the A490 value of Saos-2 cells in si-NC group and si-PTBP1 group was 1.00±0.05 and 0.55±0.07, respectively, and the difference was statistically significant ( t = 10.46, P < 0.001). The result of TUNEL staining showed that the apoptosis rate was (1.26±0.05)% and (12.86±0.87)%, respectively in si-NC group and si-PTBP1 group, and the difference was statistically significant ( t = 26.62, P < 0.001). Western blotting showed that PTEN relative expression level of Saos-2 cell in si-PTBP1 group was higher than that in si-NC group (1.02±0.05 vs. 0.26±0.04, t = 23.74, P < 0.001), and the relative expression level of p-Akt in si-PTBP1 group was lower than that in si-NC group (0.22±0.03 vs. 1.00±0.07, t = 20.48, P < 0.001). In the solvent control+si-NC, solvent control+si-PTBP1, and SF1670+si-PTBP1 groups, the relative expression level of p-Akt protein was 0.96±0.07, 0.21±0.02, and 0.79±0.03, respectively, and the difference was statistically significant ( F = 299.30, P < 0.001), and the relative expression level of PTEN protein was 0.26±0.02, 0.98±0.08, and 0.96±0.11, respectively, and the difference was statistically significant ( F = 106.80, P < 0.001). The A490 values were 1.00±0.10, 0.53±0.08, and 0.89±0.08, respectively in the solvent control+si-NC, solvent control+si-PTBP1, and SF1670+si-PTBP1 groups, and the difference was statistically significant ( F = 31.81, P < 0.001); apoptosis rates were (1.27±0.11)%, (13.26±0.89)%, and (4.86±0.37)%, respectively, and the difference was statistically significant ( F = 482.90, P < 0.001). Conclusions:Knockdown of PTBP1 inhibits the proliferation and promotes the apoptosis of osteosarcoma cells, and this effect may be associated with the regulation of the PTEN-Akt pathway.

Objective:To investigate the clinical effects of the anterolateral thigh perforator flaps transplantation in repairing the wounds after radical resection of skin malignant tumors in the head, face, and neck.Methods:This study was a retrospective observational study. From May 2020 to December 2023, 27 patients with skin malignant tumors in the head, face, and neck were admitted to the Department of Burns and Plastic Surgery of the First People's Hospital of Kashi Prefecture, including 19 males and 8 females, aged 53 to 89 years. There were 21 cases with squamous cell carcinoma and 6 cases with basal cell carcinoma. The wound area after radical resection of tumors was 9.0 cm×7.0 cm to 27.0 cm×21.5 cm. The wounds were repaired with the lobulated, combined, or various forms of combination of the anterolateral thigh perforator flaps, and the harvesting flap area in single donor site was 10.0 cm×8.0 cm to 27.0 cm×11.0 cm. The wounds in the flap donor sites were closed by suturing in 26 patients, while the wound in the flap donor site in 1 patient was repaired with medium-thickness skin graft in the lower leg. The survival of the flap, and the occurrence of vascular crisis and infection were observed after surgery. During follow-up after surgery, the tumor recurrence, shape and texture of the flaps, and the function and scar formation of the limbs where the donor site was located were observed.Results:Only one patient developed venous crisis of the flap 27 hours after surgery, and the flap survived after vascular exploration and reanastomosis of the vein; the flaps in the other patients survived after surgery. One patient had an infected effusion under the flap after surgery, which healed after dressing change. After 6-36 months of postoperative follow-up, no tumor recurrence was observed; the flap had good appearance, texture, and elasticity; the limb where the donor site was located functioned normally, with only linear scars left.Conclusions:For complex wounds after radical resection of skin malignant tumors in the head, face, and neck, different forms of anterolateral thigh perforator flaps can be used to repair the wounds according to the condition of the wounds, and the wounds in the recipient sites heal well after surgery, with minimal damage to the donor site.

Objective:To investigate the clinical effects of the anterolateral thigh perforator flaps transplantation in repairing the wounds after radical resection of skin malignant tumors in the head, face, and neck.Methods:This study was a retrospective observational study. From May 2020 to December 2023, 27 patients with skin malignant tumors in the head, face, and neck were admitted to the Department of Burns and Plastic Surgery of the First People's Hospital of Kashi Prefecture, including 19 males and 8 females, aged 53 to 89 years. There were 21 cases with squamous cell carcinoma and 6 cases with basal cell carcinoma. The wound area after radical resection of tumors was 9.0 cm×7.0 cm to 27.0 cm×21.5 cm. The wounds were repaired with the lobulated, combined, or various forms of combination of the anterolateral thigh perforator flaps, and the harvesting flap area in single donor site was 10.0 cm×8.0 cm to 27.0 cm×11.0 cm. The wounds in the flap donor sites were closed by suturing in 26 patients, while the wound in the flap donor site in 1 patient was repaired with medium-thickness skin graft in the lower leg. The survival of the flap, and the occurrence of vascular crisis and infection were observed after surgery. During follow-up after surgery, the tumor recurrence, shape and texture of the flaps, and the function and scar formation of the limbs where the donor site was located were observed.Results:Only one patient developed venous crisis of the flap 27 hours after surgery, and the flap survived after vascular exploration and reanastomosis of the vein; the flaps in the other patients survived after surgery. One patient had an infected effusion under the flap after surgery, which healed after dressing change. After 6-36 months of postoperative follow-up, no tumor recurrence was observed; the flap had good appearance, texture, and elasticity; the limb where the donor site was located functioned normally, with only linear scars left.Conclusions:For complex wounds after radical resection of skin malignant tumors in the head, face, and neck, different forms of anterolateral thigh perforator flaps can be used to repair the wounds according to the condition of the wounds, and the wounds in the recipient sites heal well after surgery, with minimal damage to the donor site.

Objective:To investigate the effect of polypyrimidine tract binding protein 1 (PTBP1) on the proliferation and apoptosis of osteosarcoma cell lines via the phosphatase and tensin homolog (PTEN)-protein kinase B (Akt) signaling pathway.Methods:Osteosarcoma cell lines MG63, U2-OS, Saos-2, 143B, and the osteoblast cell line hFOB1.19 were selected. Western blotting was used to detect the relative expression level of PTBP1 protein, and the Saos-2 cell line with the highest PTBP1 relative expression level was selected for subsequent experiments. Saos-2 cells were divided into 5 groups: si-NC group [transfected with negative control siRNA (NC siRNA)], si-PTBP1 group (transfected with PTBP1 siRNA), solvent control+si-NC group [treated with dimethyl sulfoxide(DMSO) to a final concentration of 0.1% and transfected with NC siRNA], solvent control+si-PTBP1 group (treated with 0.1% DMSO and transfected with PTBP1 siRNA), and SF1670+si-PTBP1 group (treated with 10 μmol/L SF1670, a PTEN inhibitor, and transfected with PTBP1 siRNA). CCK-8 assay was used to detect the cell proliferation [expressed as the absorbance value at 490 nm ( A490)]; TUNEL staining was used to detect apoptosis ability, and apoptosis rate was calculated; Western blotting was used to detect the expression levels of relevant proteins. Results:The relative expression level of PTBP1 protein in osteosarcoma MG63, U2-OS, Saos-2, 143B, and osteoblast cell line hFOB1.19 cells was 0.80±0.12, 0.55±0.08, 1.04±0.10, 0.49±0.07, and 0.26±0.05, respectively, and the difference was statistically significant ( F = 47.10, P < 0.001). CCK-8 assay result showed that the A490 value of Saos-2 cells in si-NC group and si-PTBP1 group was 1.00±0.05 and 0.55±0.07, respectively, and the difference was statistically significant ( t = 10.46, P < 0.001). The result of TUNEL staining showed that the apoptosis rate was (1.26±0.05)% and (12.86±0.87)%, respectively in si-NC group and si-PTBP1 group, and the difference was statistically significant ( t = 26.62, P < 0.001). Western blotting showed that PTEN relative expression level of Saos-2 cell in si-PTBP1 group was higher than that in si-NC group (1.02±0.05 vs. 0.26±0.04, t = 23.74, P < 0.001), and the relative expression level of p-Akt in si-PTBP1 group was lower than that in si-NC group (0.22±0.03 vs. 1.00±0.07, t = 20.48, P < 0.001). In the solvent control+si-NC, solvent control+si-PTBP1, and SF1670+si-PTBP1 groups, the relative expression level of p-Akt protein was 0.96±0.07, 0.21±0.02, and 0.79±0.03, respectively, and the difference was statistically significant ( F = 299.30, P < 0.001), and the relative expression level of PTEN protein was 0.26±0.02, 0.98±0.08, and 0.96±0.11, respectively, and the difference was statistically significant ( F = 106.80, P < 0.001). The A490 values were 1.00±0.10, 0.53±0.08, and 0.89±0.08, respectively in the solvent control+si-NC, solvent control+si-PTBP1, and SF1670+si-PTBP1 groups, and the difference was statistically significant ( F = 31.81, P < 0.001); apoptosis rates were (1.27±0.11)%, (13.26±0.89)%, and (4.86±0.37)%, respectively, and the difference was statistically significant ( F = 482.90, P < 0.001). Conclusions:Knockdown of PTBP1 inhibits the proliferation and promotes the apoptosis of osteosarcoma cells, and this effect may be associated with the regulation of the PTEN-Akt pathway.