Objective: To explore the learning curve of transurethral 450 nm blue light vaporization of the prostate (TUBVP) in a district hospital，in order to provide reference for clinicians who plan to perform TUBVP. Methods: The clinical data of 56 patients with benign prostatic hyperplasia (BPH) who received TUBVP performed by the same group of surgeons in Chang'an District Hospital during Jun. and Dec. 2023 were retrospectively analyzed. Cumulative sum (CUSUM) was used to fit the learning curve of ratio of volume to operating time (RVOT) of prostate volume /450 nm blue light. The learning curve was divided into different stages according to the inflection points，and the clinical data of patients operated at different stages were analyzed and compared. Results: The learning curve of TUBVP was 21 cases，including 1-21 cases in the learning stage，22-38 cases in the improvement stage and 39-56 cases in the maturity stage. With the increase of cases，the postoperative bladder irrigation time reduced \[40.00 (26.00，44.50) h vs. 23.00(20.50，34.00) h vs. 23.50(14.75，40.75) h\]，with statistical difference (P<0.05). The surgical efficiency increased \[(0.51±0.14) vs. (0.55±0.17) vs. (0.63±0.23)\]，while the reduction of hemoglobin \[(6.43±7.35) g/L vs. (5.65±10.91) g/L vs. (2.61±7.36) g/L\]，catheter indwelling time \[70.0 (66.0，106.0) h vs. 71.0 (66.0，89.0) h vs. 66.0 (58.5，78.5) h\]，and incidence of complications (9.5% vs. 5.9% vs. 0) in the three stages showed a gradually decreasing trend，but with no statistical significance (P>0.05). Conclusion: The learning curve of TUBVP is 21 cases. For clinicians in district hospitals，TUBVP is a worthy choice.

ObjectiveTo investigate the alleviating effect of calcium cyanamide （CaCN₂） soil fumigation on replanting problems of Rehmannia glutinosa. MethodsNewly soil （NP） was used as the control group， while three treatment groups were established： replanted soil （RP）， newly soil treated with CaCN₂ （120 g·m²， tillage depth 25 cm） （NPCC）， and replanted soil treated with CaCN₂ （RPCC）. R. glutinosa was cultivated in all groups. At harvest， the tuber agronomic traits （number of enlarged roots， maximum root diameter， fresh weight， dry weight） were measured. The content of catalpol and rehmannioside D was quantified by ultra-high-performance liquid chromatography （UPLC） to evaluate medicinal quality. Rhizosphere soil available nutrients and enzyme activities were analyzed by assay kits. The community structure and composition of fungi and bacteria in rhizosphere soil were assessed via internal transcribed spacer 2 （ITS2） sequencing and 16S rDNA sequencing， respectively. ResultsCompared with NP， the RP group showed obviously reduced in tuber agronomic traits and quality indicators （P0.05）. However， the RPCC group showed significant improvement in agronomic traits and a notable increase in rehmannioside D content compared to RP （P0.05）. The contents of available phosphorus and potassium in RPCC and NP groups were obviously lower than those in RP （P0.05）. The polyphenol oxidase soil （S-PPO） activity in RP was obviously lower than in NP （P0.05）， while sucrose soil （S-SC）， acid phosphatase soil （S-ACP）， and S-PPO activities in RPCC were obviously higher than in RP （P0.05）. Microbial richness and diversity in RP were obviously higher than in NP （P0.05）， whereas no significant differences were observed between the RPCC and NP. The relative abundances of fungal genera Nectria， Myrothecium， Tomentella， and bacterial genus Skermanella were obviousl lower in RPCC and NP than in RP （P0.05）. Correlation analysis that S-ACP activity was positively correlated with the content of rehmannioside D （P0.05）. Fungal genera Engyodontium and Alternaria， and bacterial genera Pir4 lineage， Pirellula， Methyloversatilis， Brevundimonas， Ralstonia， and Acidibacter were obviously positively correlated with tuber dry weight （P0.05）. Conversely， fungal genera Pseudaleuria， Nectria， Haematonectria， Ceratobasidium， and bacterial genera Streptomyces， Skermanella， RB41， Gemmatimonas， and Bacillus were obviously negatively correlated with dry weight （P0.05）. The fungal genus Alternaria and bacterial genera Brevundimonas， Ralstonia， Acidibacter， and Dongia showed positive correlations with medicinal quality of R.glutinosa tuber， while fungal genera Pseudaleuria， Nectria， Stachybotrys， Fusarium， Gibberella， Ceratobasidium， and bacterial genera Sphingomonas， Skermanella， RB41， Gemmatimonas， and Bacillus were obviously negatively correlated （P0.05）. ConclusionCaCN₂ soil fumigation can significantly improve enzyme activities in replanted Rehmannia rhizosphere soil， enhance the utilization of available nutrients， reshape microbial community structure of replanted R.glutinosa at the family and genus level， and notably improve tuber agronomic traits and medicinal quality. This study provides a novel approach to alleviating replanting problems and offers insights for the integrated development of standardized cultivation techniques， including soil disinfection， nutrient-targeted regulation， and microbial inoculant application.

Given the increasing concern regarding antibacterial resistance, the antimicrobial properties of naphthoquinones have recently attracted significant attention. While 1,4-naphthoquinone and its derivatives have been extensively studied, the antibacterial properties of 5,8-dihydroxy-1,4-naphthoquinone derivatives remain relatively unexplored. This study presents a comprehensive in vitro and in vivo analysis of the antibacterial activity of 35 naturally sourced and chemically synthesized derivatives of 5,8-dihydroxy-1,4-naphthoquinone. Kirby-Bauer antibiotic testing identified three compounds with activity against methicillin-resistant Staphylococcus aureus (MRSA), with one compound (PNP-02) demonstrating activity comparable to vancomycin in minimum inhibitory concentration, minimum bactericidal concentration (MBC), and time-kill assays. Microscopic and biochemical analyses revealed that PNP-02 adversely affects the cell wall and cell membrane of MRSA. Mechanistic investigations, including proteomic sequencing analyses, Western blotting, and RT-qPCR assays, indicated that PNP-02 compromises cell membrane integrity by inhibiting arginine biosynthesis and pyrimidine metabolism pathways, thereby increasing membrane permeability and inducing bacterial death. In an in vivo mouse model of skin wound healing, PNP-02 exhibited antibacterial efficacy similar to vancomycin. The compound demonstrated low toxicity to cultured human cells and in hemolysis assays and remained stable during serum incubation. These findings suggest that PNP-02 possesses promising bioactivity against MRSA and represents a potential novel antibacterial agent.
Methicillin-Resistant Staphylococcus aureus/genetics* ; Anti-Bacterial Agents/chemistry* ; Naphthoquinones/administration & dosage* ; Animals ; Microbial Sensitivity Tests ; Mice ; Humans ; Staphylococcal Infections/microbiology* ; Molecular Structure

ObjectiveTo establish a rapid method for evaluating the heterozygosity of Murraya paniculata germplasm materials and provide as a foundation for developing germplasm breeding and innovation measures for M. paniculata. MethodSingle nucleotide polymorphisms （SNPs） were screened from the genome resequencing data of 65 plants of M. paniculata. A self-written script was used to transform 20 SNPs into restriction fragment length polymorphism （RFLP） markers. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was employed to detect the 20 RFLP markers in 12 M. paniculata germplasm accessions， and the heterozygosity of M. paniculata germplasm accessions was calculated based on the number of enzyme-cutting bands at the 20 RFLP marker sites. Plink was used to calculate the whole genome heterozygosity of 12 M. paniculata germplasm accessions， and the results obtained with different methods were compared. ResultThere was no significant difference in the heterozygosity calculated by the PCR-RFLP method and the genome resequencing method. The PCR-RFLP and genome resequencing methods identified 8 and 9 germplasm accessions， respectively， with a heterozygosity level less than 30%. Seven germplasm accessions with heterozygosity less than 30.00% were calculated by both methods. ConclusionThe PCR-RFLP method established in this study for evaluating the heterozygosity of M. paniculata germplasm demonstrates the precision of 87.5% and the accuracy of 77.8%. This method serves as a reference for developing heterozygosity evaluation methods in other medicinal plant germplasm resources.

Objective It aims to introduce the Policy Modeling Consistency(PMC)index model for the quantitative assessment of medical and preventive integration policies,with the goal of providing reference for optimizing the poli-cies in China.Methods Using"medical and preventive integration"as the keyword,it retrieved 30 policy texts from the official websites of the central government and relevant departments.Based on the ROST CM 6.0 software and the PMC index model,it conducted a quantitative assessment of Chinese medical and preventive integration policy texts.Results The mean PMC index of the medical and preventive integration policy text is 4.552,with 8 policies clas-sified as an acceptable level.Conclusion It is necessary to strengthen the top-level design of medical and preventive integration policies,optimize the combined application of policy tools,and effectively utilize the advantages of Tradi-tional Chinese Medicine and the integration between traditional Chinese and Western medicine in medical and preven-tive integration services.

Objective It aims to introduce the Policy Modeling Consistency(PMC)index model for the quantitative assessment of medical and preventive integration policies,with the goal of providing reference for optimizing the poli-cies in China.Methods Using"medical and preventive integration"as the keyword,it retrieved 30 policy texts from the official websites of the central government and relevant departments.Based on the ROST CM 6.0 software and the PMC index model,it conducted a quantitative assessment of Chinese medical and preventive integration policy texts.Results The mean PMC index of the medical and preventive integration policy text is 4.552,with 8 policies clas-sified as an acceptable level.Conclusion It is necessary to strengthen the top-level design of medical and preventive integration policies,optimize the combined application of policy tools,and effectively utilize the advantages of Tradi-tional Chinese Medicine and the integration between traditional Chinese and Western medicine in medical and preven-tive integration services.

Objective It aims to introduce the Policy Modeling Consistency(PMC)index model for the quantitative assessment of medical and preventive integration policies,with the goal of providing reference for optimizing the poli-cies in China.Methods Using"medical and preventive integration"as the keyword,it retrieved 30 policy texts from the official websites of the central government and relevant departments.Based on the ROST CM 6.0 software and the PMC index model,it conducted a quantitative assessment of Chinese medical and preventive integration policy texts.Results The mean PMC index of the medical and preventive integration policy text is 4.552,with 8 policies clas-sified as an acceptable level.Conclusion It is necessary to strengthen the top-level design of medical and preventive integration policies,optimize the combined application of policy tools,and effectively utilize the advantages of Tradi-tional Chinese Medicine and the integration between traditional Chinese and Western medicine in medical and preven-tive integration services.

Objective It aims to introduce the Policy Modeling Consistency(PMC)index model for the quantitative assessment of medical and preventive integration policies,with the goal of providing reference for optimizing the poli-cies in China.Methods Using"medical and preventive integration"as the keyword,it retrieved 30 policy texts from the official websites of the central government and relevant departments.Based on the ROST CM 6.0 software and the PMC index model,it conducted a quantitative assessment of Chinese medical and preventive integration policy texts.Results The mean PMC index of the medical and preventive integration policy text is 4.552,with 8 policies clas-sified as an acceptable level.Conclusion It is necessary to strengthen the top-level design of medical and preventive integration policies,optimize the combined application of policy tools,and effectively utilize the advantages of Tradi-tional Chinese Medicine and the integration between traditional Chinese and Western medicine in medical and preven-tive integration services.

Objective It aims to introduce the Policy Modeling Consistency(PMC)index model for the quantitative assessment of medical and preventive integration policies,with the goal of providing reference for optimizing the poli-cies in China.Methods Using"medical and preventive integration"as the keyword,it retrieved 30 policy texts from the official websites of the central government and relevant departments.Based on the ROST CM 6.0 software and the PMC index model,it conducted a quantitative assessment of Chinese medical and preventive integration policy texts.Results The mean PMC index of the medical and preventive integration policy text is 4.552,with 8 policies clas-sified as an acceptable level.Conclusion It is necessary to strengthen the top-level design of medical and preventive integration policies,optimize the combined application of policy tools,and effectively utilize the advantages of Tradi-tional Chinese Medicine and the integration between traditional Chinese and Western medicine in medical and preven-tive integration services.

Objective It aims to introduce the Policy Modeling Consistency(PMC)index model for the quantitative assessment of medical and preventive integration policies,with the goal of providing reference for optimizing the poli-cies in China.Methods Using"medical and preventive integration"as the keyword,it retrieved 30 policy texts from the official websites of the central government and relevant departments.Based on the ROST CM 6.0 software and the PMC index model,it conducted a quantitative assessment of Chinese medical and preventive integration policy texts.Results The mean PMC index of the medical and preventive integration policy text is 4.552,with 8 policies clas-sified as an acceptable level.Conclusion It is necessary to strengthen the top-level design of medical and preventive integration policies,optimize the combined application of policy tools,and effectively utilize the advantages of Tradi-tional Chinese Medicine and the integration between traditional Chinese and Western medicine in medical and preven-tive integration services.