OBJECTIVE To evaluate the potential of nanopore sequencing technology for rapid diagnosis of Talaro-myces marneffei(TM)infection.METHODS Nine patients with TM infection admitted to the Fourth People's Hospital of Nanning from May 13,2022 to Aug.3,2023 were retrospectively analyzed.Rapid diagnosis was con-ducted by nanopore sequencing technology,and a comprehensive analysis of their clinical characteristics and treat-ment processes was performed.RESULTS The 9 patients included in the study had infections in various sites such as the lungs,buttocks,blood and cervical lymph nodes.Comorbidities included AIDS,secondary pulmonary tuberculosis,non-tuberculous mycobacterial disease and adult-onset immune deficiency.Patients generally exhibited elevated C-reactive protein levels and erythrocyte sedimentation rates,along with increased neutrophil counts.Some patients had abnormal lymphocyte counts and CD4+/CD8+ratios.Microbiological tests showed positive cultures in 3 cases,positive smears in 2 cases and positive targeted detection in 6 cases.Nanopore sequencing detected various pathogens in the 9 patients.The treatment results indicated that 8 patients improved after medication,with 6 patients having medication regimens adjusted based on nanopore sequencing results.CONCLUSION Nanopore sequencing technology has shown potentials in the auxiliary diagnosis of TM infection,providing timely etiological diagnostic evidence for clinical practice.

Objective To evaluate the efficacy of an information-based collaborative special improvement management model in reducing the incidences of falls,hospital-acquired pressure injuries(HAPI)and unplanned extubation among inpatients,hence to offer an effective management strategy.Methods This before-after controlled study included 779,249 inpatients from 1st January,2018 to 31st December,2023.A total 360,484 inpatients were assigned to the pre-implementation group(January 2018-December 2020)and received conventional nursing safety management,while 418,765 inpatients were assigned to the post-implementation group(January 2021-December 2023)and managed with an information-based collaborative special improvement management model.The two groups were compared with total incidence of nursing-related adverse events,rates of falls,HAPI,and unplanned extubation.Results In comparison with the pre-implementation group,the post-implementation group showed significant reductions in rates of total nursing-related adverse event(from 0.0960%to 0.0602%,P<0.01),HAPI(all unavoidable pressure injury)(from 0.0122%to 0.0050%,P<0.01),and unplanned extubation(from 0.0584‰ to 0.0297‰,P<0.01).Falling rate decreased from 0.0267‰ to 0.0263‰,but without significantly different(P>0.05).All four indicators demonstrated an annually downward trends after implementation of the information-based collaborative special improvement management model.Conclusion The information-based collaborative special improvement management model effectively reduces major nursing-related adverse events and enhances the safety of inpatient.

Objective To evaluate the efficacy of an information-based collaborative special improvement management model in reducing the incidences of falls,hospital-acquired pressure injuries(HAPI)and unplanned extubation among inpatients,hence to offer an effective management strategy.Methods This before-after controlled study included 779,249 inpatients from 1st January,2018 to 31st December,2023.A total 360,484 inpatients were assigned to the pre-implementation group(January 2018-December 2020)and received conventional nursing safety management,while 418,765 inpatients were assigned to the post-implementation group(January 2021-December 2023)and managed with an information-based collaborative special improvement management model.The two groups were compared with total incidence of nursing-related adverse events,rates of falls,HAPI,and unplanned extubation.Results In comparison with the pre-implementation group,the post-implementation group showed significant reductions in rates of total nursing-related adverse event(from 0.0960%to 0.0602%,P<0.01),HAPI(all unavoidable pressure injury)(from 0.0122%to 0.0050%,P<0.01),and unplanned extubation(from 0.0584‰ to 0.0297‰,P<0.01).Falling rate decreased from 0.0267‰ to 0.0263‰,but without significantly different(P>0.05).All four indicators demonstrated an annually downward trends after implementation of the information-based collaborative special improvement management model.Conclusion The information-based collaborative special improvement management model effectively reduces major nursing-related adverse events and enhances the safety of inpatient.

OBJECTIVE To evaluate the potential of nanopore sequencing technology for rapid diagnosis of Talaro-myces marneffei(TM)infection.METHODS Nine patients with TM infection admitted to the Fourth People's Hospital of Nanning from May 13,2022 to Aug.3,2023 were retrospectively analyzed.Rapid diagnosis was con-ducted by nanopore sequencing technology,and a comprehensive analysis of their clinical characteristics and treat-ment processes was performed.RESULTS The 9 patients included in the study had infections in various sites such as the lungs,buttocks,blood and cervical lymph nodes.Comorbidities included AIDS,secondary pulmonary tuberculosis,non-tuberculous mycobacterial disease and adult-onset immune deficiency.Patients generally exhibited elevated C-reactive protein levels and erythrocyte sedimentation rates,along with increased neutrophil counts.Some patients had abnormal lymphocyte counts and CD4+/CD8+ratios.Microbiological tests showed positive cultures in 3 cases,positive smears in 2 cases and positive targeted detection in 6 cases.Nanopore sequencing detected various pathogens in the 9 patients.The treatment results indicated that 8 patients improved after medication,with 6 patients having medication regimens adjusted based on nanopore sequencing results.CONCLUSION Nanopore sequencing technology has shown potentials in the auxiliary diagnosis of TM infection,providing timely etiological diagnostic evidence for clinical practice.

Objective:To explore the current situation of nursing human caring in hospital wards and analyze its influencing factors, so as to facilitate the development of nursing human caring practice.Methods:From July to November 2022, a total of 107 hospitals were surveyed through stratified convenience sampling method, and 4 072 ward nursing managers were recruited to finish the general information questionnaire and the ward nursing human caring status questionnaire. The general information included the region, class and type of the hospital, etc. The ward nursing human caring status questionnaire included 38 items in 5 dimensions of nursing human caring system and process, humanistic quality and training of nursing staff, humanistic environment and facilities, human caring procedures and measures, and human caring quality evaluation and improvement, with a full score of 190 points. Descriptive statistics were used to analyze the general data, independent samples t-test, ANOVA and correlation analysis were used to analyze the factors influencing the current status of nursing human caring in the ward, while multiple linear regression analysis was used to conduct a multivariate analysis. Results:The score of nursing human caring in hospital wards was 156.91±27.78. Whether the hospital had carried out nursing human caring pilot(demonstration) wards, whether the ward had previously been a hospital nursing human caring pilot(demonstration) nursing unit, the type of ward, and whether nursing managers had participated in human caring training were the influencing factors of the implication of nursing humanistic caring in wards( P<0.05). Conclusions:The practice of nursing human caring in hospital wards is at a good level, but needs to be further strengthened. Nursing managers should take systematically strategies to promote the development of nursing human caring practice.

Objective:To understand the genetic characteristics of subgenus C human adenovirus (HAdV-C) strain isolated in Hunan in 2014.Methods:An HAdV-C strain named Hunan2014-s024 was isolated from throat swab collected from a child with severe acute respiratory infection in Changsha city of Hunan province in 2014. The whole genome sequence (WGS) was obtained by segmented amplification and sequence splicing. Database of HAdV-C was constructed with the strain from this study and the HAdV-C representative strains from GenBank. The whole genome characteristics was analyzed by using bioinformatics software.Results:Strain Hunan2014-s024 has the highest homology with the HAdV-C strain (MK041244-CHN-2006) isolated from stool sample of a healthy child in Shanxi province in 2006, and the genome of strain MK041244-CHN-2006 was used as the backbone, and penton based gene was replaced by gene fragment from MK041246-chn-2012 strain isolated from a child with acute lower respiratory tract infection in Hunan province in 2012.Conclusions:Our result indicated that 2014 Hunan strain(Hunan2014-s024)is a recombinant virus of 2006 Shanxi strain(MK041244-CHN-2006)and 2012 Hunan strain(MK041246-CHN-2012). Due to the change of pathogenicity, it is necessary to strengthen the molecular epidemiological surveillance of HAdV-C to understand its potential disease burden and public health significance.

Objective To investigate the effect of first aid nursing path under the center of chest pain on the diagnosis and treatment of patients with ST-segment elevation myocardial infarction (STEMI). Methods A total of 79 cases of STEMI patients who had been selected for emergency treatment after the establishment of the chest pain center were as the experimental group. The patient's first aid nursing path was used in the center of chest pain. A total of 159 cases of STEMI patients before the establishment of the chest pain center were as the control group, Application of traditional clinical pathway. Compared to the diagnosis and treatment effect of two groups of patients. Results The rate of completed the project within 10 min for the experimental group: the completion of electrocardiogram examination (91.1% vs 84.9% ), oral aspirin, clopidogrel, or lindinda (81.0% vs 75.5%), and laboratory medical order (79.7% vs 69.8%) were higher than those in the control group, and the difference was statistically significant (χ2=2.31, 1.68, 2.06, P < 0.05). The day of hospitalization in the experimental group was less than that of the control group (6.48±3.82 vs 9.76± 4.32), and the difference was statistically significant (t=-9.80, P < 0.05). The mortality rate during hospitalization was lower than that of the control group (5.66% vs 3.8% ), and the difference was statistically significant (χ2=-2.24, P<0.05). Conclusion The first aid nursing path in the center of chest pain can shorten the time of diagnosis and treatment of STEMI patients and improve the diagnosis and treatment effect of STEMI patients.

4-Hydroxybenzoate (4HBA) is an important chemical compound used for synthesis of liquid crystal. Production of 4HBA from renewable resources is an effective mean to solve problems such as environmental pollution and petroleum shortage. This review briefly introduces the chemical synthesis of 4HBA from oil compounds, and mainly describes the progress in 4HBA biosynthesis from renewable resources by plants and microorganisms. In most intriguing aspect of plant-based synthesis of 4HBA is the appeal of directly synthesizing a chemical from CO2. However, the glucosylation system in plant cells converting 4HBA to glucose conjugates, causing the post treatment a problem. The recombinant microorganisms produce pure 4HBA, but less efficient. A new strain of Microbulbifer has ability to naturally accumulate 4HBA from glucose. Elucidation of the metabolic pathways and regulation systems would improve 4HBA synthesis efficiency.
Alteromonadaceae ; metabolism ; Glucose ; chemistry ; Glycosylation ; Industrial Microbiology ; Metabolic Networks and Pathways ; Parabens ; metabolism ; Plants ; metabolism

Objective To discuss the application value of work flow optimization based on hospital information system to shorten waiting time , improve nursing efficiency and ensure infusion safety in infusion center.Methods Process management combined with information technology was used to optimize the unreasonable work flow in infusion center .Self-designed questionnaire was used to compare the time between patients'arrival and leaving as well as the correct rate of nurses'transcription and typing of infusion cards before and after optimization .Results The average waiting time was ( 92.15 ±70.57 ) s before optimization and (64.34 ±37.59)s after, with statistically significant difference (Z=-6.704,P<0.01).The correct rate of transcription and typing of infusion cards improved from 96.5% to 99.3%, with statistically significant difference (χ2 =11.806 , P<0.01 ) .Conclusions The work flow optimization based on hospital information system can improve nurses'working efficiency, shorten patients'waiting time, reduce adverse incidence due to wrong transcription and typing of infusion cards and ensure the drug safety , so as to give patients effective and safe treatment and nursing in the infusion center .

Objective To explore the effect of intravenous infusion flow rebuilt on the patients’safety and nursing quality in intravenous infusion center.Methods Risk safety factors about intravenous infusion process in intravenous infusion center were analyzed and the work flow were rebuilt and optimized?The satisfaction rate,the reception time and the disinfection time for tourniquets were compared before and after work flow rebuilt.Results After work flow rebuilt,the satisfaction rate raised from 92?5%to 94?5%?There were significant differences among the reception time and the disinfection time for tourniquets before and after work flow rebuilt (all P<0?05)?After work flow rebuilt,both the reception time and the time for dealing with tourniquets are shorter than before. Conclusions The rebuilt work flow can make nurses work efficiently and enhance the patients’nursing quality?