OBJECTIVE To investigate the effects of subanesthetic dose of esketamine on postoperative anxiety and recovery in patients undergoing laparoscopic cholecystectomy. METHODS A total of 200 patients scheduled for laparoscopic cholecystectomy at Suzhou Ninth Hospital Affiliated to Soochow University from January 2023 to December 2024 were randomly assigned to control group （n＝100） and observation group （n＝100）. One minute before the initiation of anesthesia， patients in the control group received intravenous injections of Propofol emulsion injection， Sufentanil citrate injection， and Succinylcholine chloride injection. On this basis， patients in the observation group received an intravenous injection of Esketamine hydrochloride injection. The anxiety status of patients in both groups was compared， along with their general intraoperative conditions （including sufentanil dosage， duration of pneumoperitoneum， operative time， anesthesia time， and extubation time）， postoperative recovery， incidence of adverse reactions， and the need for dezocine rescue analgesia. Heart rate and mean arterial pressure， entropy index （state entropy and response entropy）， inflammatory marker levels [interleukin-6 （IL-6） and C-reactive protein （CRP）]， numerical rating scale （NRS） for pain intensity were compared between the two groups at different time points. RESULTS No significant differences were found between the two groups in pneumoperitoneum duration， operative time， anesthesia time，extubation time， incidence of postoperative dry mouth， entropy index or length of stay in the post-anesthesia care unit （P＞0.05）. Compared with the control group， the observation group showed significantly lower postoperative STAI-S scores， reduced intraoperative sufentanil consumption， decreased incidence of postoperative nausea， vomiting， and shivering， the need for dezocine rescue analgesia， as well as lower plasma IL-6 and CRP levels at 24 h after surgery， and NRS （P＜0.05）. The heart rate and mean arterial pressure of patients in the observation group at the start of surgery， end of surgery， and during extubation were all significantly higher than those in the control group （P＜0.05）. CONCLUSIONS Subanesthetic dose of esketamine can effectively alleviate postoperative anxiety， reduce intraoperative opioid consumption， suppress postoperative inflammatory response， relieve postoperative pain， and promote recovery in patients undergoing laparoscopic cholecystectomy.

Objective:To construct novel radiotracers targeting CD36 and evaluate their stabilities and targeting specificities.Methods:ABT-510, an anti-angiogenic peptide that bound CD36, served as the active scaffold for the synthesis of precursor molecules BQ01, BQ02 and BQ03. These precursors were subsequently labeled with 68Ga to yield the tracers 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03, whose radiochemical purities were determined by radio-high-performance liquid chromatography. A systematic characteristics of the radiochemical and biological profiles were performed, including in vitro stability, lipid water partition coefficient (log P), target-binding affinity, pharmacokinetic behaviour, microPET/CT imaging on U87MG tumor bearing mice, biodistribution, and phosphor-screen autoradiography. The tumor/muscle ratios of three probes were compared by one-way analysis of variance (post-hoc tests with Tukey method). Results:The radiochemical purities of 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03 were all over 95%, and the radiotracers all remained stable in PBS and serum in vitro. The log P values of 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03 were -3.81±0.08, -3.60±0.03 and -3.85±0.03 respectively, indicating hydrophilicity; and blood clearance half-lives in vivo were (25.6±0.3), (38.2±0.2) and (17.5±0.2) min respectively, demonstrating rapid elimination. 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03 displayed high binding affinities toward U87MG cells, with inhibition constants ( Ki) of (4.30±0.63), (3.80±0.24) and (2.61±0.31)nmol/L, respectively. MicroPET/CT demonstrated marked tumor accumulation of 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03 at 0.5 h after administration in U87MG tumor bearing mice, with SUV max of 0.68±0.09, 0.70±0.09 and 0.89±0.05. Biodistribution results showed the tumor uptakes of 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03 were (0.35±0.09), (0.53±0.01) and (0.63±0.06) percentage activity of injection dose per gram of tissue (%ID/g), respectively. The tumor/muscle ratio of 68Ga-BQ03 was significantly higher than that of 68Ga-BQ01 or 68Ga-BQ02 ( F=161.50, all P<0.001). Conclusions:Three CD36-targeted radiotracers demonstrate good stability, strong binding affinity, and high tumor uptake. Among them, 68Ga-BQ03 shows the best imaging performance and holds promise for the precise diagnosis of CD36-positive malignant tumors.

Panax species are mostly valuable medicinal plants. While some species' seeds are sensitive to dehydration, the dehydration tolerance of seeds from other Panax species remains unclear. The phospholipase D(PLD) gene plays an important role in plant responses to dehydration stress. However, the characteristics of the PLD gene family and their mechanisms of response to dehydration stress in seeds of Panax species with different dehydration tolerances are not well understood. This study used seeds from eight Panax species to measure the germination rates and PLD activity after dehydration and to analyze the correlation between dehydration tolerance and seed traits. Bioinformatics analysis was also conducted to characterize the PnPLD and PvPLD gene families and to evaluate their expression patterns under dehydration stress. The dehydration tolerance of Panax seeds was ranked from high to low as follows: P. ginseng, P. zingiberensis, P. quinquefolius, P. vietnamensis var. fuscidiscus, P. japonicus var. angustifolius, P. japonicus, P. notoginseng, and P. stipuleanatus. A significant negative correlation was found between dehydration tolerance and seed shape(three-dimensional variance), with flatter seeds exhibiting stronger dehydration tolerance(r=-0.792). Eighteen and nineteen PLD members were identified in P. notoginseng and P. vietnamensis var. fuscidiscus, respectively. These members were classified into five isoforms: α, β, γ, δ, and ζ. The gene structures, subcellular localization, physicochemical properties, and other characteristics of PnPLD and PvPLD were similar. Both promoters contained regulatory elements associated with plant growth and development, hormone responses, and both abiotic and biotic stress. During dehydration, the PLD enzyme activity in P. notoginseng seeds gradually increased as the water content decreased, whereas in P. vietnamensis var. fuscidiscus, PLD activity first decreased and then increased. The expression of PLDα and PLDδ in P. notoginseng seeds initially increased and then decreased, whereas in P. vietnamensis var. fuscidiscus, the expression of PLDα and PLDδ consistently decreased. In conclusion, the dehydration tolerance of Panax seeds showed a significant negative correlation with seed shape. The dehydration tolerance in P. vietnamensis var. fuscidiscus and dehydration sensitivity of P. notoginseng seeds may be related to differences in PLD enzyme activity and the expression of PLDα and PLDδ genes. This study provided the first systematic comparison of dehydration tolerance in Panax seeds and analyzed the causes of tolerance differences and the optimal water content for long-term storage at ultra-low temperatures, thus providing a theoretical basis for the short-term and ultra-low temperature long-term storage of medicinal plant seeds with varying dehydration tolerances.
Seeds/metabolism* ; Panax/physiology* ; Plant Proteins/metabolism* ; Gene Expression Regulation, Plant ; Phospholipase D/metabolism* ; Plants, Medicinal/enzymology* ; Germination ; Multigene Family ; Water/metabolism* ; Dehydration ; Phylogeny

Objective To analyze the characteristics of adverse drug reactions （ADR） reported in Sixth People’s Hospital South Campus, Shanghai Jiaotong University from 2021 to 2023, to provide reference for promoting rational clinical drug use. Methods ADR data reported in our hospital were collected retrospectively, including patients’ basic information, drugs causing adverse reactions, types of adverse reactions and outcomes. Descriptive analysis methods were used to summarize and analyze the data. Results A total of 979 cases of ADR were reported in our hospital from 2021 to 2023. The highest proportion of patients with ADR occurred in the age range of 31 to 50, and more male patients （63.5%）. The top five drugs involved with adverse reactions were antibiotics （48.8%）, Chinese medicine injections（19.2%）, vitamins（7.5%）, Chinese traditional medicine（7.2%）, equine tetanus immunoglobulin（6.3%）. Among antibiotics, cefuroxime, ceftazidime and cefotiam were the majority. The organs/systems involved in all ADR were mainly skin and accessories damage （55.4%）. The clinical manifestations were rash, itching, and maculopapular rash. Conclusion From 2021 to 2023, the most common drugs causing adverse drug reactions in our hospital were mainly antibacterial drugs, and the rational clinical use of antibacterial drugs still needs to be concerned.

Objective:To construct novel radiotracers targeting CD36 and evaluate their stabilities and targeting specificities.Methods:ABT-510, an anti-angiogenic peptide that bound CD36, served as the active scaffold for the synthesis of precursor molecules BQ01, BQ02 and BQ03. These precursors were subsequently labeled with 68Ga to yield the tracers 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03, whose radiochemical purities were determined by radio-high-performance liquid chromatography. A systematic characteristics of the radiochemical and biological profiles were performed, including in vitro stability, lipid water partition coefficient (log P), target-binding affinity, pharmacokinetic behaviour, microPET/CT imaging on U87MG tumor bearing mice, biodistribution, and phosphor-screen autoradiography. The tumor/muscle ratios of three probes were compared by one-way analysis of variance (post-hoc tests with Tukey method). Results:The radiochemical purities of 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03 were all over 95%, and the radiotracers all remained stable in PBS and serum in vitro. The log P values of 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03 were -3.81±0.08, -3.60±0.03 and -3.85±0.03 respectively, indicating hydrophilicity; and blood clearance half-lives in vivo were (25.6±0.3), (38.2±0.2) and (17.5±0.2) min respectively, demonstrating rapid elimination. 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03 displayed high binding affinities toward U87MG cells, with inhibition constants ( Ki) of (4.30±0.63), (3.80±0.24) and (2.61±0.31)nmol/L, respectively. MicroPET/CT demonstrated marked tumor accumulation of 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03 at 0.5 h after administration in U87MG tumor bearing mice, with SUV max of 0.68±0.09, 0.70±0.09 and 0.89±0.05. Biodistribution results showed the tumor uptakes of 68Ga-BQ01, 68Ga-BQ02 and 68Ga-BQ03 were (0.35±0.09), (0.53±0.01) and (0.63±0.06) percentage activity of injection dose per gram of tissue (%ID/g), respectively. The tumor/muscle ratio of 68Ga-BQ03 was significantly higher than that of 68Ga-BQ01 or 68Ga-BQ02 ( F=161.50, all P<0.001). Conclusions:Three CD36-targeted radiotracers demonstrate good stability, strong binding affinity, and high tumor uptake. Among them, 68Ga-BQ03 shows the best imaging performance and holds promise for the precise diagnosis of CD36-positive malignant tumors.

Background Alcohol dependence and related health problems have attracted greater than ever attention in recent years.Alcohol dependence not only affected personal behavior control ability,but also brought adverse effect to families and society.Objective To analyze the relationship between cognitive function and personality traits among male alcohol-dependent patients with aggressive behavior,in order to provide references for improving the cognitive function of male alcohol-dependent patients with aggressive behavior.Methods Male patients with alcohol dependence attending Hunan Brain Hospital from March 2020 to March 2022 and fulfilling the International Classification of Diseases,tenth edition(ICD-10)diagnostic criteria were enrolled.According to the score of Modified Overt Aggression Scale(MOAS),participants were classified into aggressive behavior group and non-aggressive behavior group at 1∶1 ratio,each with 80 cases.The enrolled subjects were then evaluated using Revised Eysenck Personality Questionnaire Short Scale for Chinese(EPQ-RSC)and Repeatable Battery for the Assessment of Neuropsychological Status(RBANS).Pearson correlation analysis was utilized to examine the correlation between EPQ-RSC and RBANS scores among alcohol-dependent patients with aggressive behavior.Results Scores on each subscale in RBANS were all lower in aggressive behavior group than those in non-aggressive behavior group(t=2.176,2.580,2.076,2.308,2.193,P<0.05),and scores on each dimension in EPQ-RSC in aggressive behavior group were higher than those in non-aggressive behavior group(t=4.497,5.242,6.459,P<0.01).Pearson correlation analysis denoted that the scores of introversion/extroversion,neuroticism and psychoticism in EPQ-RSC were positively correlated with the scores of immediate memory,visuospatial/constructional ability,language,attention and delayed memory in RBANS among alcohol-dependent patients with aggressive behavior(r=0.294～0.482,0.362～0.511,0.265～0.475,P<0.05).Conclusion The cognitive function may have a certain correlation with personality traits in alcohol-dependent patients with aggressive behavior.

Gadopiclenol was used in adults and pediatric patients 2 years of age and older during magnetic resonance imaging(MRI)to detect and view lesions of the central nervous system(brain,spine,and associated tissues)and body(head and neck,chest,abdomen,pelvis,and musculoskeletal system)with abnormal vascular properties.Gadopiclenol is a new type of macrocyclic gadolinium-based contrast agent(GBCA).In this article,the molecular structure,principle of action,pharmacodynamics,pharmacokinetics,clinical studies,safety and other aspects of Gadopiclenol were reviewed,in order to introduce the current research status and existing achievements of Gadopiclenol.

Diabetic kidney disease(DKD)is one of the most important complications of diabetes.In recent years,domestic and foreign studies have found that mammalian target protein of rapamycin(mTOR)related signaling pathway is a classic pathway involved in the regulation of autophagy,which can achieve the therapeutic effect of DKD by targeting the autophagy pathway,and plays a crucial role in the prevention and treatment of DKD.In this paper,we reviewed the mechanism of mTOR-related signaling pathway targeted autophagy in the prevention and treatment of DKD,in order to provide a new reference and basis for clinical prevention and treatment of DKD.

On May 27,2021,the U.S.Food and Drug Administration(FDA)officially approved Lantheus'PYLARIFY?(Piflufolastat F 18,18 F-labeled imaging agent),which can be used for positron emission computed tomography(PET)of prostate-specific membrane antigen(PSMA)-positive lesions in prostate cancer patients to accurately identify prostate cancer with suspected metastasis or recurrence.Piflufolastat F 18 is approved by FDA for two indications.The first is the initial staging for suspected metastatic lesions in men with newly diagnosed prostate cancer.The second is restaging,with the goal of identifying lesions in the setting of biochem ical recurrence.

Objective To develop a GIS-based 3D playback system for the flight human-plane data to realize the fusion of pilots'airborne flight data and physiological data.Methods The 3D playback system was developed with the Browser/Server(B/S)architecture,micro-server model,Java language and Spring Cloud technology framework,which was composed of three functional modules for flight process reproduction,physiological situational awareness and critical event calibration analysis.Results The system developed achieved time synchronization and data fusion of airborne flight data and physiological data with a time synchronization frequency of 1 Hz and a refresh rate of not less than 120 frames/s.Conclusion The system developed with high safety,stability,reliability and accuracy facilitates pilot in-flight physiological monitoring and fusion and simultaneous display of airborne flight data and physiological data,which can be used as an important platform for decision-making support in flight training.[Chinese Medical Equipment Journal,2024,45(10):14-19]