Objective:To investigate effects of high-intensity interval training(HIIT)and moderate-intensity continuous training(MICT)on cardiorespiratory function,aerobic capacity,cardiac autonomic function,cardiovascular risk factors,and quality of life in patients with coronary heart disease.Method:Forty-six patients with coronary heart disease were selected and randomly divided into 23 cases in the HIIT group and 23 cases in the MICT group.The cardiac function indexes[left ventricular end-diastolic volume(LVEDV),left ventricular ejection fraction(LVEF),and stroke volume(SV)],pulmonary function in-dexes[maximal voluntary ventilation(MVV),maximal expiratory flow(PEF),and maximal expiratory pressure(MEP)],and aerobic exercise capacity indexes[peak oxygen uptake(PeakVO2),anaerobic threshold(AT),peak oxygen pulse(VO2/HR),six-minute walking distance(6MWD)],cardiac autonomic function indices[heart rate recovery(HRR-1 min),peak heart rate,resting heart rate],cardiovascular risk factor indices[body mass index(BMI),resting systolic blood pressure,resting diastolic blood pressure],and quality of life indices(selected dimensions of SF-36).Result:After training,the levels of LVEDV,LVEF,and SV were higher in the HIIT group than in the MICT group(P<0.05).After training,the levels of MVV,PEF,and MEP were higher in the HIIT group than in the MICT group(P<0.05).After training,the levels of PeakVO2,AT,VO2/HR and 6MWD were high-er in the HIIT group than in the MICT group(P<0.05).After training,HRR-1 min and peak heart rate were higher in the HIIT group than in the MICT group(P<0.05).After training,the BMI,resting systolic blood pressure,and resting diastolic blood pressure levels in the MICT group were lower than those in the HIIT group(P<0.05).After training,the SF-36 scale scores of physical function,vitality,and health transition in the HIIT group were higher than those of the MICT group(P<0.05).Conclusion:Both HIIT and MICT can effectively improve cardiopulmonary function,aerobic exercise capaci-ty,cardiac autonomic function,cardiovascular risk factors,and quality of life in patients with coronary heart disease.HIIT demonstrated superior effects in improving cardiopulmonary function,aerobic exercise capacity,cardiac autonomic function,and quality of life in patients with coronary heart disease,while MICT is more ef-fective in improving cardiovascular risk factors.

Objective:To investigate effects of high-intensity interval training(HIIT)and moderate-intensity continuous training(MICT)on cardiorespiratory function,aerobic capacity,cardiac autonomic function,cardiovascular risk factors,and quality of life in patients with coronary heart disease.Method:Forty-six patients with coronary heart disease were selected and randomly divided into 23 cases in the HIIT group and 23 cases in the MICT group.The cardiac function indexes[left ventricular end-diastolic volume(LVEDV),left ventricular ejection fraction(LVEF),and stroke volume(SV)],pulmonary function in-dexes[maximal voluntary ventilation(MVV),maximal expiratory flow(PEF),and maximal expiratory pressure(MEP)],and aerobic exercise capacity indexes[peak oxygen uptake(PeakVO2),anaerobic threshold(AT),peak oxygen pulse(VO2/HR),six-minute walking distance(6MWD)],cardiac autonomic function indices[heart rate recovery(HRR-1 min),peak heart rate,resting heart rate],cardiovascular risk factor indices[body mass index(BMI),resting systolic blood pressure,resting diastolic blood pressure],and quality of life indices(selected dimensions of SF-36).Result:After training,the levels of LVEDV,LVEF,and SV were higher in the HIIT group than in the MICT group(P<0.05).After training,the levels of MVV,PEF,and MEP were higher in the HIIT group than in the MICT group(P<0.05).After training,the levels of PeakVO2,AT,VO2/HR and 6MWD were high-er in the HIIT group than in the MICT group(P<0.05).After training,HRR-1 min and peak heart rate were higher in the HIIT group than in the MICT group(P<0.05).After training,the BMI,resting systolic blood pressure,and resting diastolic blood pressure levels in the MICT group were lower than those in the HIIT group(P<0.05).After training,the SF-36 scale scores of physical function,vitality,and health transition in the HIIT group were higher than those of the MICT group(P<0.05).Conclusion:Both HIIT and MICT can effectively improve cardiopulmonary function,aerobic exercise capaci-ty,cardiac autonomic function,cardiovascular risk factors,and quality of life in patients with coronary heart disease.HIIT demonstrated superior effects in improving cardiopulmonary function,aerobic exercise capacity,cardiac autonomic function,and quality of life in patients with coronary heart disease,while MICT is more ef-fective in improving cardiovascular risk factors.

BACKGROUND:Adipose-derived stem cels are a kind of mesenchyam stem cels with multipotent differentiation capacity, which have more advantages than bone marrow mesenchymal stem cels in tissue engineering research. OBJECTIVE: To establish a method to isolate and purify adipose-derived stem cels from human subcutaneous adipose tissues folowed byin vitro amplification and osteoblastic/adipogenic differentiation.
METHODS: Adipose-derived stem cels were isolated from human subcutaneous adipose tissue and cultured by density gradient centrifugation and adherent culture. Cel morphology and growth features were observed under inverted microscope. Adipose-derived stem cels at passages 2 and 5 were selected for viability measurement using cel counting kit-8 method, and then cel growth curves were drawn. The immunophenotype identification was analyzed by flow cytometry. Passage 5 cels underwent osteoblastic/adipogenic induction to confirm the multi-differentiation potential.
RESULTS AND CONCLUSION: (1) Using density gradient centrifugation and adherent culture method, high-purity human adipose-derived stem cels can be successfuly isolated from human adipose tissues. (2) The growth process of human adipose-derived stem cels includes stagnant phase, logarithmic phase and plateau phase, which meets the growth rhythm of normal cels. Moreover, the population doubling time is shorter. (3). Human adipose-derived stem cels are positive for stem cel-related antigens, with low immunogenicity and the multi-differentiation potential. (4) Labeling human adipose-derived stem cels with DAPI is a simple efficient labeled method, and the labeling rate is high but the cytotoxicity is low

OBJECTIVE To realize the circumstances of the antibiotic application during perioperational period and evaluate the rational application of antimicrobial agents in department of gastrointestinal Surgery in a hospital.METHODS Retrospective study for 210 patients in the department of gastrointestinal Surgery form Jan 2007 to Dec 2008 was carried out randomly.RESULTS The antibacterial agents were used in all patients during perioperation.The antibacterial agents used most frequently were Metronidazole and Cephalosporins,The combined use cases accounted for 90.5%.The average period of treatment was 7 days.The incidence of surgical site infection was 8.6%.Only 55.6% patient′s samples were examined and drug susceptibility test were detected.CONCLUSIONS The irrational applications of prophylactic antibiotitcs during the perioperative period are serious,such as the average time of using antibiotics was too long,unreasonable frequently sort changing and low rate for samples detecting.