A scientific and efficient comprehensive operation system for the transformation of clinical research achievements is an important guarantee to fully release the capability of public hospitals of the achievement transformation. A public hospital, focusing on the bottleneck problems faced in the process of transforming scientific and technological achievements, began to explore the construction of a comprehensive operation system for the transformation of clinical research achievements and implemented it throughout the hospital from October 2023. By improving the organizational structure and setting up full-time management positions; formulating supporting policies and perfecting the incentive mechanism; drawing a systematic portrait and creating a standard pathway; expanding the transformation platform and promoting coordinated development; innovating management services and strengthening digital empowerment; and highlighting publicity and education to enhance the capability of transformation, the hospital has effectively promoted the transformation of clinical research achievements. This practice can provide a reference for other hospitals to improve the management of clinical research achievements transformation and promote high-quality hospital development.

OBJECTIVE To explore the expression levels of microribonucleic acid-223(miR-223),nucleotide-bind-ing oligomerized domain-like receptor 3(NLRP3)and effect protein Caspase-1(Caspase-1)messenger ribonucleic acid(mRNA)in the thoracolumbar vertebral fracture patients with postoperative incision infections and analyze the diagnostic values.METHODS A total of 222 patients with thoracolumbar vertebral fracture who were treated in Jinan People's Hospital from Jan.2021 to Dec.2022 were enrolled in the study and were divided into the infection group with 25 cases and the no infection group with 197 cases according to the status of postoperative infections.The relative expression levels of peripheral blood miR-223,NLRP3 and Caspase-1 mRNA were detected by means of real-time fluorescent quantitative polymerase chain reaction(qPCR),the values of the three indexes in predic-tion of the postoperative incision infections were analyzed by means of receiver operating characteristic(ROC)curves,and Pearson correlation analysis was performed for the linear relationship between the miR-223,NLRP3 and Caspase-1 and the serum amyloid A(SAA)protein,monocyte chemoattractant protein-1(MCP-1)and Toll-like receptor-2(TLR-2).RESULTS There were significant differences in the levels of miR-223,NLRP3 and Caspase-1 mRNA between the infection group and the no infection group(P＜0.05);the levels of the above inde-xes of the infection group were 3.01±1.00,2.25±0.75 and 2.31±0.77,respectively.The area under the curve(AUC)of the joint detection of miR-223,NLRP3 and Caspase-1 was 0.935 in prediction of the postoperative inci-sion infections in the patients with thoracolumbar vertebral fracture,with the sensitivity 92.00％,the specificity 80.70％.There were significant differences in the levels of SAA,MCP-1 and TLR-2 between the infection group and the no infection group(P＜0.05);the levels of the above indexes of the infection group were(169.74±19.64)mg/L,(60.45±14.63)ng/L and(75.73±13.63)μg/L,respectively.The levels of miR-223,NLRP3 and Caspase-1 mRNA are positively correlated with SAA,MCP-1 and TLR-2(P＜0.05).CONCLUSIONS The chan-ges of miR-223,NLRP3 and Caspase-1 mRNA are associated with the postoperative incision infections in the pa-tients with thoracolumbar vertebral fracture.The joint detection of miR-223,NLRP3 and Caspase-1 mRNA may facilitate the diagnosis of postoperative incision infections,and the three indexes are positively correlated with SAA,MCP-1 and TLR-2.

OBJECTIVE To explore the expression levels of microribonucleic acid-223(miR-223),nucleotide-bind-ing oligomerized domain-like receptor 3(NLRP3)and effect protein Caspase-1(Caspase-1)messenger ribonucleic acid(mRNA)in the thoracolumbar vertebral fracture patients with postoperative incision infections and analyze the diagnostic values.METHODS A total of 222 patients with thoracolumbar vertebral fracture who were treated in Jinan People's Hospital from Jan.2021 to Dec.2022 were enrolled in the study and were divided into the infection group with 25 cases and the no infection group with 197 cases according to the status of postoperative infections.The relative expression levels of peripheral blood miR-223,NLRP3 and Caspase-1 mRNA were detected by means of real-time fluorescent quantitative polymerase chain reaction(qPCR),the values of the three indexes in predic-tion of the postoperative incision infections were analyzed by means of receiver operating characteristic(ROC)curves,and Pearson correlation analysis was performed for the linear relationship between the miR-223,NLRP3 and Caspase-1 and the serum amyloid A(SAA)protein,monocyte chemoattractant protein-1(MCP-1)and Toll-like receptor-2(TLR-2).RESULTS There were significant differences in the levels of miR-223,NLRP3 and Caspase-1 mRNA between the infection group and the no infection group(P＜0.05);the levels of the above inde-xes of the infection group were 3.01±1.00,2.25±0.75 and 2.31±0.77,respectively.The area under the curve(AUC)of the joint detection of miR-223,NLRP3 and Caspase-1 was 0.935 in prediction of the postoperative inci-sion infections in the patients with thoracolumbar vertebral fracture,with the sensitivity 92.00％,the specificity 80.70％.There were significant differences in the levels of SAA,MCP-1 and TLR-2 between the infection group and the no infection group(P＜0.05);the levels of the above indexes of the infection group were(169.74±19.64)mg/L,(60.45±14.63)ng/L and(75.73±13.63)μg/L,respectively.The levels of miR-223,NLRP3 and Caspase-1 mRNA are positively correlated with SAA,MCP-1 and TLR-2(P＜0.05).CONCLUSIONS The chan-ges of miR-223,NLRP3 and Caspase-1 mRNA are associated with the postoperative incision infections in the pa-tients with thoracolumbar vertebral fracture.The joint detection of miR-223,NLRP3 and Caspase-1 mRNA may facilitate the diagnosis of postoperative incision infections,and the three indexes are positively correlated with SAA,MCP-1 and TLR-2.

A scientific and efficient comprehensive operation system for the transformation of clinical research achievements is an important guarantee to fully release the capability of public hospitals of the achievement transformation. A public hospital, focusing on the bottleneck problems faced in the process of transforming scientific and technological achievements, began to explore the construction of a comprehensive operation system for the transformation of clinical research achievements and implemented it throughout the hospital from October 2023. By improving the organizational structure and setting up full-time management positions; formulating supporting policies and perfecting the incentive mechanism; drawing a systematic portrait and creating a standard pathway; expanding the transformation platform and promoting coordinated development; innovating management services and strengthening digital empowerment; and highlighting publicity and education to enhance the capability of transformation, the hospital has effectively promoted the transformation of clinical research achievements. This practice can provide a reference for other hospitals to improve the management of clinical research achievements transformation and promote high-quality hospital development.

Promoting the transformation and development of public hospitals into clinical research-oriented hospitals is the necessary way for China to deepen the reform of medicine and health system. In August 2019, Henan Cancer Hospital deeply combined the key elements of clinical research management with the concept of multidisciplinary collaborative governance, created an offline operating end of collaborative governance (including the front collaborative recruitment system, the middle collaborative quality management system, and the terminal collaborative transformation system), built a supporting internet plus intelligent auxiliary end (including the intelligent recruitment manager, the intelligent quality manager and the intelligent transformation manager), and built a clinical research intelligent management mode covering the trinity of subject recruitment, clinical research quality control, and innovation achievements transformation. The hospital focused on management elements such as " focusing on quality, improving efficiency, and increasing motivation" , and drove the front, middle, and terminal ends of the model to work together, to ensure the quality and efficiency of clinical research and achieve a comprehensive improvement in the hospital′s clinical research level. As of December 2023, the hospital had undertaken over 2 000 clinical research projects of various types, with a cumulative amount of clinical research conversion contracts exceeding 53 million yuan. This practice had achieved good results and could provided references for other tertiary public hospitals to promote high-quality development of clinical research.

For patients with partial jaw defects, cysts and dental implants, doctors need to take panoramic X-ray films or manually draw dental arch lines to generate Panorama images in order to observe their complete dentition information during oral diagnosis. In order to solve the problems of additional burden for patients to take panoramic X-ray films and time-consuming issue for doctors to manually segment dental arch lines, this paper proposes an automatic panorama reconstruction method based on cone beam computerized tomography (CBCT). The V-network (VNet) is used to pre-segment the teeth and the background to generate the corresponding binary image, and then the Bezier curve is used to define the best dental arch curve to generate the oral panorama. In addition, this research also addressed the issues of mistakenly recognizing the teeth and jaws as dental arches, incomplete coverage of the dental arch area by the generated dental arch lines, and low robustness, providing intelligent methods for dental diagnosis and improve the work efficiency of doctors.
Humans ; Radiography, Panoramic/methods* ; Cone-Beam Computed Tomography/methods* ; Head ; Image Processing, Computer-Assisted/methods*

Objective:To explore the epidemiological characteristics and the antibiotic resistance of Streptococcus pneumoniae isolates, and to provide the evidence for the rational use of antimicrobial agents to treat Streptococcus pneumoniae infection. Methods:The positive microbiological laboratory identification and antimicrobial susceptibility testing of Streptococcus pneumoniae from sputum of children with respiratory infections during January 2010 to December 2017 in Children′s Hospital of Soochow University were retrospectively analyzed. The positive rates of Streptococcus pneumoniae of different genders, ages, years and seasons were compared. The annual detection rates and trends of drug resistance of Streptococcus pneumoniae to penicillin, amoxicillin and cefotaxime were analyzed by Mann-Kendall trend test. The seasonal decomposition of time series was conducted to assess the association between Streptococcus pneumoniae detection rate and season. Enumeration data was compared using χ2 test. Results:Of the 88 480 sputum specimens, the total positive rate of Streptococcus pneumoniae was 10.3%(9 081/88 480). The detection rates of Streptococcus pneumoniae in children aged 0 to <0.5 years old, 0.5 to <2 years old, 2 to <3 years old, 3 to <5 years old, and 5 to <15 years old were 4.2%(1 407/33 224), 13.1%(3 191/24 390), 14.9%(2 417/16 252), 17.9%(1 474/8 246) and 9.3%(592/6 368), respectively. The difference was statistically significant ( χ2=2 421.6, P<0.01). The detection rates were 8.1%(1 321/16 306) from January to March, 10.9%(2 194/20 207) from April to June, 8.5%(2 141/25 058) from July to September, and 12.7%(3 425/26 909) from October to December. The discrepancy of positive rates in different seasons showed statistical significance ( χ2=311.5, P<0.01). During 2010 to 2017, significant decreases in antibiotic resistant rates of Streptococcus pneumoniae to penicillin, amoxicillin and cefotaxime were detected (tau=-0.93, -0.93 and -0.71, respectively, all P<0.05). Conclusions:The detection rate of Streptococcus pneumoniae in sputum of children with respiratory infections may present seasonal pattern and vary between different ages of children. The resistance to β-lactam antibiotics has declined.

Objective To explore the gender difference in clinical features and outcomes of elderly patients with coronary true bifurcation lesions after percutaneous coronary intervention (PCI).Methods A total of 169 consecutive patients were included in the study, who were diagnosed coronary true bifurcation lesions by coronary angiography (CAG)and received PCI in our hospital from December 2013 to December 2015.All patients were divided into 2 groups according to their gender (71 femals and 98 males).Angiographic characteristics of the coronary lesions and clinical data were analyzed in both groups.Clinical outcomes during follow up were recorded and studied.Results The levels of total cholesterol (TC), triglyceride (TG),high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) in the female group were significantly higher than in the male group (all P<0.05).The levels of BUN,Cr,UA,CyC and TBIL in the female group were significantly lower than in the male group (all P<0.05).Comorbidity rates of hypertension and diabetes were higher in female patients without significant differences (P>0.05).The median follow-up time was 17 months and the incidence rate of overall adverse events in women was higher than that of men (25.4% vs.11.2%, P<0.05).Conclusions The clinical outcomes of elderly patients with coronary true bifurcation lesions after PCI had gender differences.The rates of adverse events in female patients was found significantly higher than male patients.