Objective To explore the application effect of arthroscopic knotless suture bridge technique in the treatment of patients with large rotator cuff tear.Methods Retrospective analysis of clinical data from 213 patients with large rotator cuff tear in first department of orthopedics of Qinhuangdao Military Hospital from January 2022 to October 2023.According to different treatment methods,they were divided into conventional knot group(n=77)and knotless group(n=136).The conventional knot group was treated with arthroscopic knot suture bridge technique,while the knotless group was given arthroscopic knotless suture bridge technique.The treatment effects were compared.Postoperative complications were recorded and the occurrence of rotator cuff re-tear was followed up.Results At 6 months after treatment,the SF-MPQ pain scores in conventional knot group and knotless group significantly reduced(P＜0.05),and the Constant score,UCLASS score and ASES shoulder joint score and the angles of shoulder anteflexion,abduction,internal rotation and external rotation in both groups were significantly improved at 6 months after treatment(P＜0.05).However,there was no significant difference in SF-MPO scores,Constant,UCLASS and ASES scores between the two groups compared with those before surgery(P＞0.05).The incidence of complications and retear were 4.41％and 1.47％in the no-nodule group,and 11.69％and 6.49％in the conventional nodule group,respectively,and there was statistical significance between the two groups(P＜0.05).Conclusion Arthroscopic knot and knotless suture bridge techniques can promote joint function recovery of large rotator cuff tear,and both have high minimally invasive value.However,knotless suture bridge technique has greater advantages in reducing complications and re-tear rate.

Objective To explore the application effect of arthroscopic knotless suture bridge technique in the treatment of patients with large rotator cuff tear.Methods Retrospective analysis of clinical data from 213 patients with large rotator cuff tear in first department of orthopedics of Qinhuangdao Military Hospital from January 2022 to October 2023.According to different treatment methods,they were divided into conventional knot group(n=77)and knotless group(n=136).The conventional knot group was treated with arthroscopic knot suture bridge technique,while the knotless group was given arthroscopic knotless suture bridge technique.The treatment effects were compared.Postoperative complications were recorded and the occurrence of rotator cuff re-tear was followed up.Results At 6 months after treatment,the SF-MPQ pain scores in conventional knot group and knotless group significantly reduced(P＜0.05),and the Constant score,UCLASS score and ASES shoulder joint score and the angles of shoulder anteflexion,abduction,internal rotation and external rotation in both groups were significantly improved at 6 months after treatment(P＜0.05).However,there was no significant difference in SF-MPO scores,Constant,UCLASS and ASES scores between the two groups compared with those before surgery(P＞0.05).The incidence of complications and retear were 4.41％and 1.47％in the no-nodule group,and 11.69％and 6.49％in the conventional nodule group,respectively,and there was statistical significance between the two groups(P＜0.05).Conclusion Arthroscopic knot and knotless suture bridge techniques can promote joint function recovery of large rotator cuff tear,and both have high minimally invasive value.However,knotless suture bridge technique has greater advantages in reducing complications and re-tear rate.

Objective To establish a genetic monitoring method for laboratory quails.Methods Quail microsatellite loci were searched in the literature,and microsatellite DNA loci suitable for quails were screened by an interspecific transfer method in closely related species,namely chickens and ducks.Quail liver DNA was extracted as a template,and the corresponding loci were screened by PCR amplification and agarose gel electrophoresis.On the basis of amplification of the selected microsatellite loci,the number of alleles,polymorphisms,and microsatellite loci combinations for quail genetic quality detection were selected and detection method were developed.Results We preliminary determined 23 microsatellite loci for genetic monitoring of closed-colony laboratory quails.Conclusions A genetic monitoring method for laboratory quails was preliminary developed.

Objective:To explore the repair methods of complex facial defect wounds involving paranasal sinuses and their clinical effectiveness.Methods:A retrospective observational study was conducted. From January 2020 to May 2022, 5 patients admitted to the Department of Burns and Plastic Surgery of Xiangya Hospital of Central South University and 4 patients admitted to the Department of Burns and Plastic Surgery of Chenzhou First People's Hospital with complex facial defect wounds involving paranasal sinuses met the inclusion criteria, including 6 males and 3 females, aged 35-69 years, including 4 patients with titanium mesh exposure combined with paranasal sinuses injury and 5 patients with tumor involving paranasal sinuses. After an adequate assessment of the damage by a multiple discipline team, titanium mesh removal, paranasal sinus debridement, and paranasal sinus mucosa removal were performed in patients with exposed titanium mesh, and radical tumor resection was performed in patients with tumors, with postoperative skin and soft tissue defects areas of 5.0 cm×2.5 cm to 18.0 cm×7.0 cm, anterior paranasal sinus wall defects/absence areas of 3 cm×2 cm to 6 cm×4 cm, and sinus cavity depths of 1 to 4 cm. Depending on the perforator course of the descending branch of the lateral circumflex femoral artery, the anterolateral femoral chimeric flap or anterolateral femoral myocutaneous flap (with flap area of 9 cm×4 cm to 19 cm×8 cm, muscle size of 5 cm×3 cm×3 cm to 11 cm×6 cm×3 cm) was transplanted to repair the defect, and the donor site wound was sutured directly. The type of tissue flap transplanted, the blood vessel of the recipient area, and the vascular anastomosis way during the operation, the recovery of the donor and recipient areas and the occurrence of complications after operation were observed. The appearance and blood supply of the recipient area and the recurrence of ulcers and tumors were followed up.Results:The anterolateral femoral myocutaneous flap transplantation was performed in 6 patients, and the anterolateral femoral chimeric flap transplantation was performed in 3 patients. The blood vessels in recipient areas were facial arteries and veins in 3 cases and superficial temporal arteries and veins in 6 cases. The superficial temporal arteries and veins were bridged with blood vessels in tissue flaps by flow-through way in 2 patients, and end-to-end anastomosis of blood vessels in donor and recipient areas was performed in 7 patients. After operation, all the tissue flaps survived, and the facial defect wounds were well repaired without cerebrospinal fluid leakage or paranasal sinus secretion leakage, no intracranial infection occurred, and the wounds in donor areas were healed well. Follow-up of 6-35 months after operation showed that all the patients had good blood supply in the recipient area, and the shape was acceptable; 4 patients with exposed titanium mesh had no recurrence of ulceration, and 5 patients with tumor had no local tumor recurrence or metastasis.Conclusions:Based on an adequate assessment of the extent of paranasal sinuses involved in the facial wound and the nature of the defect, good clinical effects can be achieved by using the anterolateral femoral muscle flap or the anterolateral femoral chimeric flap transplantation to repair complex facial defect wounds with open paranasal sinuses.

Objective:The investigation and research on the application status of Hepatic Venous Pressure Gradient (HVPG) is very important to understand the real situation and future development of this technology in China.Methods:This study comprehensively investigated the basic situation of HVPG technology in China, including hospital distribution, hospital level, annual number of cases, catheters used, average cost, indications and existing problems.Results:According to the survey, there were 70 hospitals in China carrying out HVPG technology in 2021, distributed in 28 provinces (autonomous regions and municipalities directly under the central Government). A total of 4 398 cases of HVPG were performed in all the surveyed hospitals in 2021, of which 2 291 cases (52.1%) were tested by HVPG alone. The average cost of HVPG detection was (5 617.2±2 079.4) yuan. 96.3% of the teams completed HVPG detection with balloon method, and most of the teams used thrombectomy balloon catheter (80.3%).Conclusion:Through this investigation, the status of domestic clinical application of HVPG has been clarified, and it has been confirmed that many domestic medical institutions have mastered this technology, but it still needs to continue to promote and popularize HVPG technology in the future.

A 52-year-old male patient received self-purchased Yinaoning tablets (益脑宁片) (3 tablets, trice daily) orally for health care. On day 18 of medication, the patient developed jaundiced skin and sclera and dark urine. On day 24, he developed fatigue and anorexia. Laboratory tests showed alanine aminotransferase (ALT) 1 132 U/L, aspartate aminotransferase (AST) 953 U/L, total bilirubin (TBil) 80.0 μmol/L, direct bilirubin (DBil) 73.4 μmol/L, alkaline phosphatase (ALP) 203 U/L, and gamma-glutamyltransferase (γ-GT) 155 U/L. Acute liver injury induced by Yinanning tablets was considered. Then the drug was discontinued and liver-protective therapy and drugs for anti-jaundice were given. On day 6 of drug withdrawal, the laboratory tests showed ALT 192 U/L, AST 158 U/L, TBil 51.0 μmol/L, DBil 39.9 μmol/L, ALP 178 U/L, and γ-GT 149 U/L, his appetite recovered, and the yellowish skin and sclera improved markedly. On day 13 of drug withdrawal, his yellowish skin and sclera disappeared, and the laboratory tests showed ALT 56 U/L, AST 62 U/L, TBil 30.7 μmol/L, DBil 22.4 μmol/L, ALP 164 U/L, and γ-GT 142 U/L. At 6 months of follow-up, there were no abnormalities in his liver function.

A 52-year-old male patient received self-purchased Yinaoning tablets (益脑宁片) (3 tablets, trice daily) orally for health care. On day 18 of medication, the patient developed jaundiced skin and sclera and dark urine. On day 24, he developed fatigue and anorexia. Laboratory tests showed alanine aminotransferase (ALT) 1 132 U/L, aspartate aminotransferase (AST) 953 U/L, total bilirubin (TBil) 80.0 μmol/L, direct bilirubin (DBil) 73.4 μmol/L, alkaline phosphatase (ALP) 203 U/L, and gamma-glutamyltransferase (γ-GT) 155 U/L. Acute liver injury induced by Yinanning tablets was considered. Then the drug was discontinued and liver-protective therapy and drugs for anti-jaundice were given. On day 6 of drug withdrawal, the laboratory tests showed ALT 192 U/L, AST 158 U/L, TBil 51.0 μmol/L, DBil 39.9 μmol/L, ALP 178 U/L, and γ-GT 149 U/L, his appetite recovered, and the yellowish skin and sclera improved markedly. On day 13 of drug withdrawal, his yellowish skin and sclera disappeared, and the laboratory tests showed ALT 56 U/L, AST 62 U/L, TBil 30.7 μmol/L, DBil 22.4 μmol/L, ALP 164 U/L, and γ-GT 142 U/L. At 6 months of follow-up, there were no abnormalities in his liver function.

This article systematically reviewed the clinical research of Traditional Chinese Medicine (TCM) combined with Vacuum Sealing Drainage (VSD) in the treatment of refractory wounds. The results showed that TCM combined with VSD for refractory wounds was characterized by abundent treatment and effectiveness. Treatments such as oral administration of TCM decoction, injection and lavage administration of TCM decoction, and external application of TCM ointment can reduce the level of inflammation in the wound, improve local microcirculation, promote granulation tissue growth and wound healing, and improve clinical efficacy. This paper summarized the clinical researches on the treatment of refractory wounds by TCM and VSD, and provide a useful reference for clinical treatment of refractory wounds.

OBJECTIVE:To provide reference for quality evaluation of Ejiao. METHODS:The contents of 17 amino acids in 18 batches of Ejiao from 18 manufacturers were analyzed by automatic amino acid analyzer;the content data was processed by prin-cipal component analysis (PCA) and hierarchical clustering analysis (HCA),in order to understand the relationship among these amino acids and categorize the Ejiao products. RESULTS:Except for a batch of Ejiao,the other 17 batches contained 17 various amino acids,including 7 necessary amino acids(Thr,Val,Met,Ile,Leu,Phe and Lys)for human being,especially the contents of Gly and Pro were higher. The total content of amino acids was arranged from 66.1% to 82.0%,showing great difference. Four main components were extracted by PCA,the cumulative contribution of which was 89.578%,and accordingly it may be consid-ered that Pro,Ala,Glu,Asp,Leu,Ile and Thr were characteristic amino acids of Ejiao products. In hierarchical clustering analy-sis,3 tree figures of commercial Ejiao product were achieved and Euclidean distance was varied among 0-25. CONCLUSIONS:There is great difference in total control of amino acid in commercial Ejiao product;Ejiao products from various manufacturers dif-fer in their amino acid contents. Ejiao is rich in collagen,and therefore,using amino acid determination would be helpful to moni-tor its quality.

Objective To establish a detection technique for H.pylori(HP) infection in Mongolian gerbils using nested PCR technique.Methods H.pylori was cultured in vitro and inoculated into Mongolian gerbils.At the 10th week after infection, the HP in the gastric juice of Mongolian gerbil was detected by conventional PCR assay and the gastric juice, gastric mucosa, duodenal contents and colon stool were examined by nested PCR.Rapid urease test and ELISA were used to analyze the accuracy of the nested PCR assay.All of the PCR products were verified by sequencing.Results The positive rate of gastric juice detected by conventional PCR was 30%, while the positive rates of gastric juice, gastric mucosa, duodenal contents and colon stool detected by nested PCR were 100%, 100%, 90%, and 10%, respectively.The positive detection rates of rapid urease test and serum ELISA were 100% and 0%, respectively.Comparing the results of different methods, both the positive rates of gastric juice and gastric mucosa detected by nested PCR and the detection rate of rapid urease test were 100%, but the results of conventional PCR detection of gastric juice, the nested PCR detection result of stool in colon and of serum ELISA assay were lower than other methods.Conclusions Due to its high accuracy and sensitivity, the nested PCR assay of gastric juice can be used for the long-time detection of H.pylori infection in Mongolian gerbils, especially useful in the experiments of prevention and treatment of H.pylori infection.