Objective To explore the correlation between thyroid antibody levels and early renal injury in patients with type 2 diabetes mellitus(T2DM)combined with Hashimoto's thyroiditis(HT).Methods A total of 375 T2DM patients hospitalized in The Affiliated Hospital of Xuzhou Medical University from January 2018 to December 2022 were selectedas the study subjects,and 197 healthy people were selected as the control subjects.The patients with T2DM were divided into simple T2DM group(n=191)and T2DM combined with HT group(HT,n=184).According to the urinary albumin/creatinine ratio(UACR)level,T2DM patients with HT were divided into microalbuminuria subgroup(MUAlb,30≤UACR≤300 mg/g,n=70)and normal albuminuria subgroup(NUAlb,UACR<30 mg/g,n=114).According to whether the thyroid antibody was positive,they were divided into thyroid peroxides antibody[TPOAb(+)]subgroup(n=56),thyroglobulin antibody[TGAb(+)]subgroup(n=40)and TGAb and TPOAb double antibody positive subgroup(n=88).Results Compared with the NC group,the smoking,drinking,urinary creatinine,alpha 1-microglobulin,UACR,FPG,HbA1c,LDL-C,TC,and TG in the HT and T2DM groups increased(P<0.05),while HDL-C decreased(P<0.05).Compared with the NUAlb subgroup,the MUAlb subgroup showed age,DM duration,FPG,HbA1c,TGAb,TPOAb,thyroid stimulating hormone(TSH)increased(P<0.05),while FT3 and eGFR decreased(P<0.05).Spearman correlation analysis showed that UACR was positively correlated with age,HbA1c,TPOAb,TGAb,TSH(P<0.01),and negatively correlated with FT3(P<0.01).The UACR of the TGAb(+)+TPOAb(+)subgroup was higher than that of the TGAb(+)and TPOAb(+)subgroups(P<0.05).Logistic regression analysis showed that TSH,TGAb,TPOAb,and HbA1c were risk factors for MUAlb,while FT3 was a protective factor for MUAlb.Conclusions In T2DM with HT patients with normal thyroid function,TPOAb and TGAb are closely related to the occurrence of early renal injury.

Trihelix transcription factors play important roles in plant light responses, growth and development, and stress responses. However, Trihelix has not yet been reported in Eucommia ulmoides. In this study, bioinformatics methods were used to comprehensively identify and analyze the expression patterns of the Trihelix gene family in E. ulmoides, aiming to provide a basis for further functional studies of EuGTs genes. A total of 9 Trihelix gene family members were identified in E. ulmoides, encoding proteins with 339 to 883 amino acids, with isoelectric points ranging from 5.13 to 9.39 and relative molecular weights between 36 992.06 and 97 871.61. Subcellular localization results showed that only EuGT-2 was localized in chloroplasts, while the others were located in the nucleus. The Trihelix gene family was categorized into six subfamilies: GT-1, GT-2, SH4, SIP1, GTγ, and GTδ. EuGTs were distributed among three subfamilies: SH4, GT-1, and GT-2, containing 1, 6, and 2 Trihelix proteins, respectively, with 2 to 17 exons. The promoters of EuGTs contained various cis-acting elements related to hormones, stress, photoperiod, and growth and development. Collinearity analysis revealed 5 collinear gene pairs between E. ulmoides and Arabidopsis thaliana, and 14 collinear gene pairs between E. ulmoides and Populus. Expression pattern analysis showed that EuGTs exhibited tissue-specific expression: EuGT-1, EuGT-2 had the highest expression levels in leaves, EuGT-4, EuGT-6, EuGT-9 had the highest transcriptional levels in marginal peel, and EuGT-5、EuGT-8 were predominantly expressed in the xylem. As leaves developed, EuGTs showed a trend of asynchronous changes. No significant differences in EuGTs expression were observed between male and female flowers, with high expression levels mainly during the induction stage of flowering. The qRT-PCR analysis indicated that most EuGTs genes were most highly expressed in the leaves of E. ulmoides, while EuGT-5 was highly expressed in the stems. Under 200 mmol·L~(-1) NaCl treatment, most EuGTs genes exhibited an initial increase followed by a decrease in expression, significantly responding to salt stress. This study provides important genetic resources for further exploration of EuGTs gene functions and germplasm innovation in E. ulmoides.
Plant Proteins/metabolism* ; Gene Expression Regulation, Plant ; Eucommiaceae/chemistry* ; Phylogeny ; Multigene Family/genetics* ; Gene Expression Profiling ; Transcription Factors/metabolism* ; Genome, Plant/genetics*

Objective:To investigate the related risk factors related to patellar dislocation combined with osteochondral fracture (OCF).Methods:A case control study was conducted to analyze the clinical data of 278 patients with patellar dislocation admitted to Second Hospital of Lanzhou University from January 2016 to June 2020, including 98 males and 180 females; aged 12-41 years [(21.1±4.6)years]. The patients were divided into OCF group ( n=112) and non-OCF group ( n=166) according to the surgical records and occurrence of OCF or not under intraoperative arthroscopy. The data of the two groups were recorded, including basic characteristics (ie, sex, age, sides of injury, whether or not the epiphysis is closed), patella height, patellar inclination angle, tibial tubercle-trochlear groove (TT-TG), femoral trochlear shape, tibial lateral rotation angle and femoral anteversion angle. Univariate analysis was adopted to evaluate the correlation of the data with patellar dislocation combined with OCF. Multivariate Logistic regression analysis was used to determine the independent risk factors of patellar dislocation combined with OCF. Results:Univariate analysis showed that patellar dislocation combined with OCF was related to male, patellar inclination angle and femoral anteversion angle ( P<0.05 or 0.01), while not to age, epiphysis is closed or not, sides of injury, patella height, TT-TG, femoral trochlear shape and tibial lateral rotation angle (all P>0.05). Multivariate Logistic regression analysis showed that male ( OR=1.75, 95% CI 1.07-2.90, P<0.05) and increased femoral anteversion angle ( OR=3.12, 95% CI 1.89-5.17, P<0.05) were significantly correlated with patellar dislocation combined with OCF. Conclusion:Male and increased femoral anteversion angle are independent risk factors for patellar dislocation combined with OCF.

Objective To evaluate the clinical effect and safety of oxycodone hydrochloride in the anesthesia for percutaneous radiofrequency ablation (PRFA) in hepatocellular carcinoma.Methods Between March and December 2015,60 cases of hepatocellular carcinoma patients undergoing percutaneous radiofrequency ablation surgery in Peking University Cancer Hospital were randomly divided into three groups:oxycodone group (group Q),fentanyl group (group F) and dezocine group (group D),20 cases in each group.Respectively intravenously injection oxycodone 0.1 mg/kg,fentanyl 0.001 mg/kg,dezocine 0.1 mg/kg before surgery.After the surgeon completed puncture administer propofol to maintain anesthesia.Recorded mean arterial pressure (MAP),heart rate (HR),respiratory rate (RR),oxygen saturation (SpO2) changes in each group at entrance,beginning of radiofrequency ablation (T1),radiofrequency ablation began after 10 minutes (T2),the end of the surgical and awake.Observe the analgesia effect,respiratory depression,nausea,vomit and other complications.Postoperative pain scores were recorded.Using ANOVA,repeated measure variance analysis,SNK test,x2 test and other tests to evaluate the anesthetic effect indexes.Results The observation completed in all patients.Patients of three groups had no significant differences in general information.No significant difference between MAP,HR and SpO2 at each time points among the three groups.At the T1 time point (group Q:(11.7 ± 1.6)/min,group D:(12.1 ±l.7)/min,group F:(10.3 ±2.3)/min,F=5.068,P =0.009) and T2 time point (group Q:(11.9±1.3)/min,group D:(12.2±1.4)/min,group F:(1O.7±.1.3)/min,F=7.024,P=0.O02),RR in group F were lower than in group Q and group D.Pain visual analogue scores after waking (group Q:0.2 ±0.7,group D:O.3 ±0.7,group F:1.7 ± 1.5,F=12.981,P =0.000) and postoperative pain score of 1 hour (group Q:2.0 ± 0.9,group D:1.8 ± O.8,group F:4.3 ± 0.9,F =42.362,P =0.000) in the group Q and group D were significantly lower than in group F.The body movements in group Q and group D were significantly less than in group F (3 cases,3 cases,9 cases,x2 =6.400,P =0.041).Intraoperative respiratory depression in group Q and group D were lower than group F (3 cases,2 cases,9 cases,x2 =8.012,P =0.018).Conclusions Oxycodone hydrochloride can be used safely and effectively for radiofrequency ablation.It has favorable hemodynamic stability,lower incidence of respiratory depression,and advantage in terms of postoperative pain.

Objective To evaluate the clinical effect and safety of oxycodone hydrochloride in the anesthesia for percutaneous radiofrequency ablation (PRFA) in hepatocellular carcinoma.Methods Between March and December 2015,60 cases of hepatocellular carcinoma patients undergoing percutaneous radiofrequency ablation surgery in Peking University Cancer Hospital were randomly divided into three groups:oxycodone group (group Q),fentanyl group (group F) and dezocine group (group D),20 cases in each group.Respectively intravenously injection oxycodone 0.1 mg/kg,fentanyl 0.001 mg/kg,dezocine 0.1 mg/kg before surgery.After the surgeon completed puncture administer propofol to maintain anesthesia.Recorded mean arterial pressure (MAP),heart rate (HR),respiratory rate (RR),oxygen saturation (SpO2) changes in each group at entrance,beginning of radiofrequency ablation (T1),radiofrequency ablation began after 10 minutes (T2),the end of the surgical and awake.Observe the analgesia effect,respiratory depression,nausea,vomit and other complications.Postoperative pain scores were recorded.Using ANOVA,repeated measure variance analysis,SNK test,x2 test and other tests to evaluate the anesthetic effect indexes.Results The observation completed in all patients.Patients of three groups had no significant differences in general information.No significant difference between MAP,HR and SpO2 at each time points among the three groups.At the T1 time point (group Q:(11.7 ± 1.6)/min,group D:(12.1 ±l.7)/min,group F:(10.3 ±2.3)/min,F=5.068,P =0.009) and T2 time point (group Q:(11.9±1.3)/min,group D:(12.2±1.4)/min,group F:(1O.7±.1.3)/min,F=7.024,P=0.O02),RR in group F were lower than in group Q and group D.Pain visual analogue scores after waking (group Q:0.2 ±0.7,group D:O.3 ±0.7,group F:1.7 ± 1.5,F=12.981,P =0.000) and postoperative pain score of 1 hour (group Q:2.0 ± 0.9,group D:1.8 ± O.8,group F:4.3 ± 0.9,F =42.362,P =0.000) in the group Q and group D were significantly lower than in group F.The body movements in group Q and group D were significantly less than in group F (3 cases,3 cases,9 cases,x2 =6.400,P =0.041).Intraoperative respiratory depression in group Q and group D were lower than group F (3 cases,2 cases,9 cases,x2 =8.012,P =0.018).Conclusions Oxycodone hydrochloride can be used safely and effectively for radiofrequency ablation.It has favorable hemodynamic stability,lower incidence of respiratory depression,and advantage in terms of postoperative pain.

OBJECTIVE: To investigate the distribution of positive allergens in patients with allergic rhinitis in Changzhou. METHOD: A total of 4242 cases with allergic symptoms and signs underwent skin prick test with 16 kinds of standardized allergens. The common allergens were found out. The samples were stratified on seven age groups to analysis the distributive characteristics of allergens responsible for each group. The samples were also stratified on children group and adults group to analysis their response degree to dust mite. RESULT: 2638 cases (62.19%) had the positive reaction, including male 1380 (52.3%) cases, female 1258 (47.7%) cases. Dermatophagoides farinae and dermatophagoides pteronyssinus had the highest positive ratio (85.86%, 83.55%) in inhalation group, followed cockroach (11.49%) and Humulus L. (9.82%), fungus (2.92%) in the lower positive ratio. Shrimp (5.76%) was the most common allergen in food group. The distribution of common allergens in different age groups was variable. There was significantly higher sensitivity to dust mite between children group compared to adults group (P < 0.01). CONCLUSION The study shows that Dust mite was the mostly responsible common allergen in Changzhou district. The distribution of common allergens in children group and adults group was variable.
Adolescent ; Adult ; Aged ; Allergens ; analysis ; Animals ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Male ; Middle Aged ; Mites ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; diagnosis ; epidemiology ; Skin Tests ; Young Adult