Objective:To further study on the biological characteristics of vaccinia virus VG9 strain obtained by passaging from vaccinia virus Tiantan strain(VTT).Methods:The freeze-dried vaccinia virus VG9 strain was reconstituted and inoculated into Vero cells for recovery and sequential passaging. The strain was identified by indirect immunofluorescence and PCR amplification. The whole genome of VG9 was sequenced using next-generation sequencing technology. The sequencing results were compared with the reference sequence of VTT and the genome sequences of other 33 orthopoxviral strains, and a phylogenetic tree was drawn. A purified clone of VG9, namely VG9-V3-3, was obtained by terminal dilution method. Preliminary studies were conducted to characterize the biological properties of this clone, such as virus titer and intradermal virulence in rabbit.Results:VG9 could specifically bind to the rabbit antibody of VTT by indirect immunofluorescence identification, and the PCR amplification results proved that this strain contained the characteristic TK gene fragment of vaccinia virus. Whole-genome sequencing showed that the genome length of VG9 was 183 596 bp, including 165 150 bp in coding region. The sequencing results were compared with the NCBI core nucleotide database, and showed that the sequences of VG9 and multiple existing Tiantan isolates were highly homologous (consistency>99.5%). Sequence comparison revealed a total of 749 nucleotide site differences between the gene sequences of VG9 and TP5 isolate of VTT. The viral titer of the VG9-V3-3 isolate was 6.3 lg (PFU/ml), and the rabbit intradermal virulence assay showed that its virulence was significantly weakened compared with that of VTT.Conclusions:The biological characteristics of vaccinia virus VG9 strain are preliminarily studied and a purified clone strain named VG9-V3-3 is obtained. The viral titer of the isolate is basically stable, and the virulence is significantly weakened compared with that of VTT. In-depth study on the immunogenicity of VG9-V3-3 will be carried out in the future to explore its feasibility as a vaccine production strain.

Objective:To further study on the biological characteristics of vaccinia virus VG9 strain obtained by passaging from vaccinia virus Tiantan strain(VTT).Methods:The freeze-dried vaccinia virus VG9 strain was reconstituted and inoculated into Vero cells for recovery and sequential passaging. The strain was identified by indirect immunofluorescence and PCR amplification. The whole genome of VG9 was sequenced using next-generation sequencing technology. The sequencing results were compared with the reference sequence of VTT and the genome sequences of other 33 orthopoxviral strains, and a phylogenetic tree was drawn. A purified clone of VG9, namely VG9-V3-3, was obtained by terminal dilution method. Preliminary studies were conducted to characterize the biological properties of this clone, such as virus titer and intradermal virulence in rabbit.Results:VG9 could specifically bind to the rabbit antibody of VTT by indirect immunofluorescence identification, and the PCR amplification results proved that this strain contained the characteristic TK gene fragment of vaccinia virus. Whole-genome sequencing showed that the genome length of VG9 was 183 596 bp, including 165 150 bp in coding region. The sequencing results were compared with the NCBI core nucleotide database, and showed that the sequences of VG9 and multiple existing Tiantan isolates were highly homologous (consistency>99.5%). Sequence comparison revealed a total of 749 nucleotide site differences between the gene sequences of VG9 and TP5 isolate of VTT. The viral titer of the VG9-V3-3 isolate was 6.3 lg (PFU/ml), and the rabbit intradermal virulence assay showed that its virulence was significantly weakened compared with that of VTT.Conclusions:The biological characteristics of vaccinia virus VG9 strain are preliminarily studied and a purified clone strain named VG9-V3-3 is obtained. The viral titer of the isolate is basically stable, and the virulence is significantly weakened compared with that of VTT. In-depth study on the immunogenicity of VG9-V3-3 will be carried out in the future to explore its feasibility as a vaccine production strain.

Objective:To investigate the incidence of musculoskeletal pain among the officers and sailors of a large warship and to analyze the related risk factors, so as to provide a scientific reference for effective preventive strategies.Methods:Based on the research design of cross-section study and correlation study, the demographic information, working and training status, and the cases of musculoskeletal pain of officers and sailors were studied by questionnaire, then the risk factors were analyzed by logistic regression.Results:Among the 501 participants, within one year, the most frequently occurred pains were pain in the lower back (375 cases, 74.9%), pain in the knee (354 cases, 70.7%), and pain in the back of the neck (336 cases, 67.1%). The influencing factor analysis of the pains on these three body parts revealed that age, job, on-board working years, on-board working unit, times of moving heavy objects per week, time of sitting when working each day, and times of assisting production and construction per week were the risk factors for musculoskeletal pain in different body parts.Conclusion:The incidence of musculoskeletal pain is very high among naval officers and sailors, so the health education, health screening, and treatment should be carried out timely. Appropriate measures should be taken to eliminate those correctable risk factors, so as to improve the health and working performance of the officers and sailors.

Objective:To investigate the incidence of musculoskeletal pain among the officers and sailors of a large warship and to analyze the related risk factors, so as to provide a scientific reference for effective preventive strategies.Methods:Based on the research design of cross-section study and correlation study, the demographic information, working and training status, and the cases of musculoskeletal pain of officers and sailors were studied by questionnaire, then the risk factors were analyzed by logistic regression.Results:Among the 501 participants, within one year, the most frequently occurred pains were pain in the lower back (375 cases, 74.9%), pain in the knee (354 cases, 70.7%), and pain in the back of the neck (336 cases, 67.1%). The influencing factor analysis of the pains on these three body parts revealed that age, job, on-board working years, on-board working unit, times of moving heavy objects per week, time of sitting when working each day, and times of assisting production and construction per week were the risk factors for musculoskeletal pain in different body parts.Conclusion:The incidence of musculoskeletal pain is very high among naval officers and sailors, so the health education, health screening, and treatment should be carried out timely. Appropriate measures should be taken to eliminate those correctable risk factors, so as to improve the health and working performance of the officers and sailors.

Objective: To compare the safety and immunogenicity of two different sequential schedules of inactivated poliomyelitis vaccine made from Sabin strain (sIPV) followed by typeⅠ+Ⅲ bivalent oral poliovirus vaccine (bOPV) in Drug Candy (DC) form or liquid dosage form). Methods: This randomized, blinded, single center, parallel-group controlled trial was done from September 2015 to June 2016 in Liuzhou, Guangxi province. Healthy infants aged ≥2 months were eligible for enrollment and divided into 1sIPV+2bOPV or 2sIPV+1bOPV sequential schedules. According to the bOPV dosage form each sequential schedules, the subjects again were divided into drug candy(DC) form or liquid dosage form group, being 1sIPV+bOPV (DC)/1sIPV+2bOPV(liquid)/2sIPV+1bOPV(DC)/2sIPV+1bOPV(liquid). According to 0, 28, 56 d immunization schedule, Each group were given 3 doses. We recorded adverse events during the clinical trial (399 participants who receive at least one dose). 28 days post-Dose 3, we receive a total of 350 blood samples (excluding the quitters or subjects against trial plan), using cell culture trace against polio virus neutralization test Ⅰ, Ⅱ, Ⅲ neutralizing antibody (GMT), calculating the antibody positive rate.PolioⅠ,Ⅱand Ⅲ antibody titers were assessed by virus-neutralizing antibody assay and the seroconversion (4-fold increase in titer) from pre-Dose 1 to 28 days post-Dose 3 was calculated (total 350 samples) . Results: During the vaccination, the incidence of AEs in 1sIPV+2bOPV(DC), 1sIPV+2bOPV (liquid), 2sIPV+1bOPV(DC), 2sIPV+1bOPV (liquid) group were 79%, 76%, 80% and 74% (χ²=1.23, P=0.747) , respectively. The severe AEs in groups were 6%, 5%, 6% and 4% (χ²=0.57, P=0.903) , respectively, and none was considered to be vaccination related. 28 days after 3^rd vaccination, the seroconversion rates in 1sIPV+2bOPV (DC), 1sIPV+2bOPV (liquid), 2sIPV+1bOPV (DC), 2sIPV+1bOPV (liquid) group, were 99%, 100%, 99% and 99% (χ²=0.94, P=0.815) , respectively, for type Ⅰ poliovirus; and 47%, 57%, 80%, 79% (χ²=31.56, P<0.001) , respectively, for type Ⅱ; and were 100%, 99%, 100%, 99% (χ²=2.02, P=0.568) , respectively, for type Ⅲ. In each group, the GMT of antibody against poliovirus typeⅠ were 4 539.68, 6 243.43, 6 819.53 and 7 916.29 (F=25.87, P<0.001) , respectively; Type Ⅱ were 12.98, 10.54, 63.75 and 84.21 (F=8.68, P=0.034) , respectively; Type Ⅲ were 1 172.55, 1 416.03, 2 648.89 and 3 250.75 (F=14.50, P=0.002) , respectively. Conclusion On the same sequential schedules, there was no significant difference between the dosage forms, all of them showed good safety and immunogenicity. In the same dosage forms with different sequential schedules, the seroconversion rate was higher in 2 dose sIPV group than the 1 dose sIPV group, especially at the neutralizing antibody GMT level against polio type Ⅱ and Ⅲ after vaccination.

Objective To investigate the association between 5-hydroxytryptamine 2A receptor (HTR2A)gene T102C locus polymorphism and schizophrenia,and to provide basis for evidence-based medicine for the genetic background of schizophrenia.Methods PubMed,EMbase,CNKI,WanFang and Vip information databases were used to search full text of all the relevant studies about the association between HTR2A gene T102C locus polymorphism and schizophrenia,which were published during 2003 to 2012.Based on reviewing full text,the data were selected, evaluated and accessed. RevMan 5.1 and Stata 1 2.0 were used to perform the statistical analysis of those studies that were in accordance with the inclusive criteria. According to the different ethnicities, the obj ects were divided into two subgroups as European and Asian to analyze respectively. Also, depending on different inheritances, the obj ects were divided into five patterns including C/T allele, CC/TT, CC/CT+TT, CC+CT/TT and CC+ TT/CT genotypes to analyze respectively, including heterogeneity inspection, effect consoliating and publication bias assessment. Results A total of 11 studies were available for this analysis, including 2 443 schizophrenia patients and 2 469 controls.The Meta-analysis results showed that the allele of all people were OR=1.12,95%CI=0.96-1.31,P>0.05;CC/TT of all people were OR=1.11,95%CI=0.80-1.53,P>0.05;CC/CT+TT of all people were OR=1.13,95%CI=0.99-1.30,P>0.05;CC+CT/TT of all people were OR=1.18, 95%CI=0.93-1.50,P>0.05;CC+TT/CT of all people were OR=0.95, 95%CI=0.84-1.06,P>0.05.Conclusion Current evidence is insufficient to show that HTR2A gene T102C locus polymorphism may be associated with schizophrenia, suggesting that the gene polymorphism has no significantly genetic association with schizophrenia.

Objective To investigate the nutritional risks in hospitalized patients with liver diseases and work out nursing countermeasures.Methods Forty patients with liver cirrhosis, 40 patients with primary liver cancer and another 40 revisiting after liver transplantation involved in the investigation with a self-designed general information questionnaire and the nutritional risk screening 2002. The three groups were compared in terms of nutritional risks.Results Among the total 120 patients,38.3%(46/120)of them took the nutritional risk and even 12.5%(15/120)had the risk of undernourishment.The risk in the patients with liver cirrhosis was higher than that in the other two groups(χ2=9.899 and 11.4299 and P=0.002 and 0.001,respectively).Conclusions The nursing staffs should pay attention to the nutritional status of patients with liver diseases, especially the patients with liver cirrhosis.It is necessary to take effective measures in order to improve the nutritional status of the patients to reduce the nutritional risks.

Objective To investigate the effect of puerarin and compound salvia injection on P2selectin change in patients with traumatic cerebral infarction(TCI)so as to understand the difference of their curative effect. Methods Twenty patients with TCI were randomly divided into four groups,ie,conventional treatment group,puerarin group,compound salvia injection group and combined treatment group to observe the improvement of the symptoms(Glasgow Outcome Scale,GOS)and change of plasma P2 selecfin after treatment.Results The symptoms(GOS score)and level of plasma P2 selectin in the puerarin group,the compound salvia injection group and the combined treatment group were superior to those of the conventional treatment group(P<0.05).The TCI patients had obviously higher level of plasma P2 Selectin than the healthy persons before treatment.The level of plasma P2 selectin was decreased after treatment,with better curative effect in three other groups compared with the conventional treatment group. Conclusions There is positive synergistic interaction between puerarin and compound salvia injection.Both puerarin and compound salvia injection Can decrease the level of plasma P2 Selectin to reduce the blood viscosity,inhibit the platelet activation,reduce the cerebral vasospasm and inhibit the thrombosis,as contributes to the recovery of the TCI patients.

Objective To evaluate the control status and knowledge level about disease in asthmatic patients in region level cities of Shaanxi province for effect appraisal of patient education.Methods Eight hospitals were selected from six region level cities, where questionnaire survey was completed in out-patients with asthma (≥ 14 years old).Results A total of 523 patients completed the questionnaire with a ratio of male to female of 1: 1.14,and an average age of (44.3±15.5) years old.The percentage of controlled, partly controlled and uncontrolled by self-evaluation was respectively 26.4%, 52.4% and 11.1%.48% insisted on using inhaled corticosteroids (ICS).The average score was 17.88±4.43 by asthma control test (ACT).The first three medicines used daily were ICS (26.6%), sustained-release theophyline (25.2%) and combination ICS/long-acting β2-agonists (21.8%).12.6% had no medicine and 5.2% used non-orthodox medicines.68.6% patients had omen before exacerbation, and those were sneezing, chest distress and cough.73.6% knew asthma is a disease of airway inilammatian,and 33.3% selected ICS as the leader medicine.Only 32.1% attended the lecture about asthma in hospitals and 85.0% longed for such education.Conclusions The control status and knowledge level about disease in asthmatic patients in cities still need to be improved in Shaanxi province, and too much work need to be done in order to realize the total control in all patients.

BACKGROUND: Recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF) could stimulate the proliferation of fibroblasts, keratinocyte and skin mucosae cells to different degrees.OBJECTIVE: To observe the effect of rhGM-CSF on the healing of drug exosmose induced skin ulcers.DESIGN: A randomized and controlled animal experiment.SETTING: Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University of Chinese PLA.MATERIALS: This experiment was carried out at the Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University of Chinese PLA from June to November 2004. Totally 20 male Kunming white mice, with body mass of 18 to 24 g, were chosen.METHODS: Prepared skin ulcers animal models were randomly divided into control group and treated group with 10 white mice in each group.Mice in the control group were given 1mg phentolamine ,20 mg lidocaine and 1mg dexamethasone diluted by normal saline to 0.5 mL ,then sealed up , once a day for 7 days; 25 μg rhGM-CSF was diluted by normal saline to 0.5 mL , then the solution was injected into the periphery of ulcers of mice in treated group , once every other day, for 7 days. Healing time and histological change of skin tissue at ulcer were observed.MAIN OUTCOME MEASURES: To observe the effect of rhGM-CSF on the healing time of drug exosmose induced skin ulcer and anabrosis and histological changeRESULTS: Totally 20 mice entered the stage of result analysis. ①Healing time: the healing time of ulcer and erosion was significantly longer in control group than in treated group [(20-24,8-12)d,t=2.264,P=0.01];②Histological observation: hyperplasia of granulation tissue was not obviously on 7 days after treatment in control group; Hyperplasia of granulation tissue appeared and the newly born blood vessel was abundant on 7 days after treatment in the treated group.CONCLUSION: rhGM-CSF can promote the wound healing of drug induced anabrosis and ulcer.