The comparison between traditional Chinese medicine Jinzhen oral liquid (JZOL) and Western medicine in treating children with acute bronchitis (AB) showed encouraging outcomes. This trial evaluated the efficacy and safety of the JZOL for improving cough and expectoration in children with AB. 480 children were randomly assigned to take JZOL or ambroxol hydrochloride and clenbuterol hydrochloride oral solution for 7 days. The primary outcome was time-to-cough resolution. The median time-to-cough resolution in both groups was 5.0 days and the antitussive onset median time was only 1 day. This randomized controlled trial showed that JZOL was not inferior to cough suppressant and phlegm resolving western medicine in treating cough and sputum and could comprehensively treat respiratory and systemic discomfort symptoms. Combined with clinical trials, the mechanism of JZOL against AB was uncovered by network target analysis, it was found that the pathways in TRP channels like IL-1β/IL1R/TRPV1/TRPA1, NGF/TrkA/TRPV1/TRPA1, and PGE2/EP/PKA/TRPV1/TRPA1 might play important roles. Animal experiments further confirmed that inflammation and the immune regulatory effect of JZOL in the treatment of AB were of vital importance and TRP channels were the key mechanism of action.

Objective? To understand the status quo of professional autonomy, sense of organizational support and professional benefit of stroke specialist nurses, and to analyze the relationship between them. Methods? Using the convenient sampling method, from January to July 2018, a total of 91 stroke specialist nurses from six Class Ⅲ Grade A hospitals in Hangzhou and Wenzhou were selected for the study. The General Data Questionnaire, Professional Autonomy Scale, Organizational Support Scale, and Questionnaire of Nurse' Perceived Professional Benefits were used to collect information about them. Data analysis was performed using independent sample t-test and one-way ANOVA, Pearson correlation analysis, and structural equation model. A total of 91 questionnaires were distributed in this study, and 86 valid questionnaires were used. The effective recovery rate of the questionnaire was 94.51%. Results? A total of 86 stroke specialist nurses got a total average score of (4.47±0.49) in Professional Autonomy Scale, an total average score of (3.02±0.36) in Organizational Support Scale, a total score of (115.56±20.46) in Questionnaire of Nurse' Perceived Professional Benefits;There were statistical differences between stoke specialist nurses with difference genders and titles in terms of their professional benefits(P＜ 0.05). Professional autonomy of stroke specialist nurses plays a mediating role in organizational support and professional benefit, with mediating effect accounting for 32.5% of the total effect. Conclusions? Professional autonomy, job involvement and organizational support affect professional benefit of stroke specialist nurses. Professional autonomy plays a mediating role in organizational support and professional benefit.

Aim To investigate the effect and mecha-nism of quercetin combined with cisplatin on prolifera-tion and apoptosis of human osteosarcoma cell line MG-63 . Methods MG-63 cells were treated with quercetin alone or combined with cisplatin. Cellular morphologic changes were observed under inverted phase contrast microscope. The effects of proliferation inhibition were assayed by CCK-8 method. The combination effect was judged through Chou-Talaly analysis. The apoptosis ra-tios of cells were analyzed by flow cytometry. The gene expression of Bcl-2 and caspase-3 was detected by RT-PCR assay. The protein expression of Bcl-2 and caspase-3 was measured by Western blot assay. Re-sults Quercetin alone or combined with cisplatin could inhibit the proliferation, but induce the apoptosis of MG-63 cells. Combination of quercetin and cisplatin revealed a synergistic effect on cell proliferation and apoptosis as it reduced the expression of Bcl-2 but en-hanced that of caspase-3 at both gene and protein lev-els. Conclusion Synergistic effect of quercetin com-bined with cisplatin on cell proliferation and apoptosis of MG-63 cells is possibly due to reduction of Bcl-2 and enhancement of caspase-3 expression.