Objectives:To investigate the mechanism of insulin-like growth factor 1(IGF-1)in mediating intervertebral disc cell aging through regulating the activity of the silent information regulator sirtuin 1(SIRT1).Methods:The intervertebral disc tissues of eight-week old female Spargue Dawley(SD)rats were dissected,from which annulus fibrosus(AF)cells were isolated and cultured in vitro.The effects of IGF-1 on the aging of AF cells were studied by β-galactosidase(SA-β-Gal)staining,cell proliferation assays,and cell migration experiments,in exposure to different concentrations of recombinant rat IGF-1(0,1,10,50,100ng/mL)in culture.The molecular mechanism of IGF-1 on AF cells was further explored by Western blot analysis.Results:After treatment with exogenous IGF-1 at different concentrations,SA-β-Gal staining and western blot results showed that IGF-1 promoted AF cell aging in a concentration-dependent manner.The CCK-8 analysis showed that low concentrations of IGF-1 significantly induced AF cell proliferation.Transwell assay results showed that low concentrations of IGF-1 profoundly enhanced the migratory capability of AF cells,whereas IGF-1 at high concentrations demonstrated no significant effect on AF cell migration.Western blot results showed that IGF-1 could downregulate the expression of SIRT1 and elevate the acetylation of p53.Conclusions:IGF-1 can promote AF cell aging though inhibiting SIRT1 expression and activity.

OBJECTIVE: To review the research progress of three-dimensional (3D) bioprinting technology for wound dressing design and preparation. METHODS: The literature on 3D bioprinted wound dressings in recent years, both domestically and internationally, was retrieved. The core principles of 3D bioprinting technology, mainstream methods, and their applications in wound dressings design and preparation were summarized. RESULTS: By leveraging precise spatial manipulation capabilities and multi-material integration, 3D bioprinting technology constructs the functionalized wound dressings with complex structures and bioactivity. These dressings primarily function across several dimensions: wound hemostasis, infection control, controlled drug release, and monitoring wound healing. CONCLUSION Although 3D bioprinted wound dressings can promote wound healing through multiple dimensions, large-scale clinical validation is still lacking. Future efforts should further clarify their clinical value and scope of application to provide more efficient, precise, and patient-comfortable treatment options for refractory wounds .
Humans ; Wound Healing ; Printing, Three-Dimensional ; Bioprinting/methods* ; Bandages ; Tissue Engineering/methods* ; Tissue Scaffolds ; Biocompatible Materials

Objectives:To investigate the mechanism of insulin-like growth factor 1(IGF-1)in mediating intervertebral disc cell aging through regulating the activity of the silent information regulator sirtuin 1(SIRT1).Methods:The intervertebral disc tissues of eight-week old female Spargue Dawley(SD)rats were dissected,from which annulus fibrosus(AF)cells were isolated and cultured in vitro.The effects of IGF-1 on the aging of AF cells were studied by β-galactosidase(SA-β-Gal)staining,cell proliferation assays,and cell migration experiments,in exposure to different concentrations of recombinant rat IGF-1(0,1,10,50,100ng/mL)in culture.The molecular mechanism of IGF-1 on AF cells was further explored by Western blot analysis.Results:After treatment with exogenous IGF-1 at different concentrations,SA-β-Gal staining and western blot results showed that IGF-1 promoted AF cell aging in a concentration-dependent manner.The CCK-8 analysis showed that low concentrations of IGF-1 significantly induced AF cell proliferation.Transwell assay results showed that low concentrations of IGF-1 profoundly enhanced the migratory capability of AF cells,whereas IGF-1 at high concentrations demonstrated no significant effect on AF cell migration.Western blot results showed that IGF-1 could downregulate the expression of SIRT1 and elevate the acetylation of p53.Conclusions:IGF-1 can promote AF cell aging though inhibiting SIRT1 expression and activity.

Objective To investigate the imaging features in CT/MR of pancreatic neuroendocrine tumors(PNETs) with multiple lesions and further deepen the understanding of this disease .Methods A retrospective review of 12 PNETs patients′radiological data with pancreatic tumors′numbers≥2 and confirmed by surgery or fine needle aspiration biopsy in Changhai Hospital were conducted .Five cases underwent pancreatic CT plain and enhanced scan , 2 cases underwent MRI plain and enhanced scan , and 5 cases underwent both CT and MRI scan .Results There were totally 46 lesions in 12 patients.There were 29 (63.0%) lesions located in the pancreatic head and neck , and 17(37.0%) lesions located in body and tail of pancreas.The sizes of the lesions ranged from 0.8 to 9.5 cm,and the median size was 2.9 cm.Forty-four (95.7%) of the tumors was round or oval , and 2 ( 4.3%) was lobulated;44 ( 95.7%) mass solid and 2 (4.3%) was cystic.CT plain scan detected punctate , crescent or nodular calcification in 8(17.4%) lesions;enhanced scan found 42 lesions(91.4%) were markedly enhanced in the arterial phase , 2 lesions (4.3%) were markedly enhanced in the pancreatic phase;2 lesions (4.3%) were slightly enhanced and the degree of enhancement was lower than that of the normal pancreas .Four cases (33.3%) had dilatation of pancreatic duct and/or the bile duct, 4 cases (33.3%) had distant organ metastasis, 2 cases (16.7%) had lymph node metastasis, and 3 cases (25.0%) had vascular invasion .Conclusions PNETs can be multiple and vary in the size.Most of the lesions are round or oval solid lesions and the malignant signs for organ metastasis can be found occasionally .In dynamic enhanced scanning , the obvious enhancement of the solid portion in the tumor and the higher enhancement degree than that of normal pancreas is the main characteristic .

Objective To analyze the constituent of the 32 kD protein band and its expression in schizophrenia se?rum. Methods Sixty schizophrenia patients and 58 health controls were recruited. The serum samples were collected and precipitated with 7%PEG. The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to ob?tain the abnormal 32 kD proteins band in patients. This protein band was cut and then analyzed using mass spectrometric technique. Results The 32 kD protein band was present in 38 schizophrenia patients but not in control and positive rate was 63.33%. The mass spectrometric analysis showed that 32 kD protein band contained 14 proteins ranging from 30 kD to 35 kD, including 6 high-frequency proteins (cDNA coded protein 1 and 2, Apolin protein A-1, Isoform 2 of ficolin-2, Complement factor H and clusterin) and 8 low-frequency proteins (IgG H chain, zinc-alphg-2-glycoprotein, fermitin,family apolin protein L-1, isoform 10 of collectin-1, purine nucleoside, anne xin and cDNA coded protein 3). Three cD?NA coded unknown proteins were highly similar to complement C4-B, β2-glycoprotein and erythrocyte band 7 integral membrane protein. Conclusion There is a unknown specific 32 kD protein that is consisted mainly of fourteen proteins in serum of schizophrenia.