Objective To explore the impact of number of positive regional lymph nodes (nPRLN) in N1 stage on the prognosis of non-small cell lung cancer (NSCLC) patients. Methods Patients with TxN1M0 stage NSCLC who underwent lobectomy and mediastinal lymph node dissection from 2010 to 2015 were screened from SEER database (17 Regs, 2022nov sub). The optimal cutoff value of nPRLN was determined using X-tile software, and patients were divided into 2 groups according to the cutoff value: a nPRLN≤optimal cutoff group and a nPRLN>optimal cutoff group. The influence of confounding factors was minimized by propensity score matching (PSM) at a ratio of 1 : 1. Kaplan-Meier curves and Cox proportional hazards models were used to evaluate overall survival (OS) and lung cancer-specific survival (LCSS) of patients. Results A total of 1316 patients with TxN1M0 stage NSCLC were included, including 662 males and 654 females, with a median age of 67 (60, 73) years. The optimal cutoff value of nPRLN was 3, with 1165 patients in the nPRLN≤3 group and 151 patients in the nPRLN>3 group. After PSM, there were 138 patients in each group. Regardless of before or after PSM, OS and LCSS of patients in the nPRLN≤3 group were superior to those in the nPRLN>3 group (P<0.001). N1 stage nPRLN>3 was an independent prognostic risk factor for OS [HR=1.52, 95%CI (1.22, 1.89), P<0.001] and LCSS [HR=1.72, 95%CI (1.36, 2.18), P<0.001]. Conclusion N1 stage nPRLN>3 is an independent prognostic risk factor for NSCLC patients in TxN1M0 stage, which may provide new evidence for future revision of TNM staging N1 stage subclassification.

Objective This study reports on the obligatory parasitism of water mites Arrenurus sp. on Anopheles messeae at the Manzhouli Port, Inner Mongolia, China. Methods Duing July 2024, a survey on the mosquito diversity was conducted at the Manzhouli Port. Captured mosquitoes and their ectoparasites were identified to species level. Results A total of 1840 adult mosquitoes were collected, representing species from three genera: Culex(Cx. modestus, Cx. pipiens pallens), Aedes(Ae. dorsalis, Ae. flavidorsalis, Ae. flavescens), and Anopheles (An. messeae). Among all the mosqutioes specimens,3 out of 150 captured An. messeae were found to carry ectoparasitic mites, with number of 2,4,27 mites separately. Morphological and molecular identification reached the same result as water mites(Hydrachnidiae, Hydracrina). COI gene sequence showed 94% similarity with the closest species Arrenurus truncatellus. Conlusions Literature review suggests water mites are host-specific parasitism of mosquito species and herein with the first record of Arrenurus sp. parasiting on An. Messeae in the most high-latitude region globally.

Anal stenosis can be categorized into congenital and acquired types based on etiology. Congenital anal stenosis falls under the spectrum of congenital anorectal malformations， being resulted from abnormal embryonic development. Acquired stenosis is not an independent disease but rather a secondary manifestation of various anorectal conditions or injuries， with primary etiologies being primary tumors and local scar formation in the anorectum. Currently， the diagnosis and treatment for anal stenosis both domestically and internationally face challenges of inconsistent standards and significant variability in management strategies. In particular， there is a lack of systematic guidance on the standard application of diagnosis and treatment models with integrated traditional Chinese and Western medicine. Focusing on cicatricial anal stenosis， this guideline was developed by the project team through a comprehensive process including expert interviews， clinical investigation， evidence retrieval and screening， evidence evaluation， formulation of recommendations， guideline drafting， self-assessment and solicitation of feedback， and professional society review. It is based on the best available evidence-based medical data and fully incorporates expert clinical experience. The guideline aims to standardize the clinical diagnosis and treatment process for cicatricial anal stenosis with integrated traditional Chinese and Western medicine， providing clinicians with standardized management strategies and methods. This document covers the etiology， pathology， diagnostic classification， integrated traditional Chinese and Western medicine treatment， and preventive care. By incorporating the characteristics of syndrome differentiation and treatment in traditional Chinese medicine， it establishes personalized， stepped treatment protocols， thereby benefiting more patients with anal stenosis and improving their quality of life and prognosis.

Hypertrophic cardiomyopathy (HCM) is a major contributor to cardiovascular diseases (CVD), the leading cause of death globally. HCM can precipitate heart failure (HF) by causing the cardiac tissue to weaken and stretch, thereby impairing its pumping efficiency. Moreover, HCM increases the risk of atrial fibrillation, which in turn elevates the likelihood of thrombus formation and stroke. Given these significant clinical ramifications, research into the etiology and pathogenesis of HCM is intensifying at multiple levels. In this review, we discuss and synthesize the latest findings on HCM pathogenesis, drawing on key experimental studies conducted both in vitro and in vivo. We also offer our insights and perspectives on these mechanisms, while highlighting the limitations of current research. Advancing fundamental research in this area is essential for developing effective therapeutic interventions and enhancing the clinical management of HCM.
Cardiomyopathy, Hypertrophic/physiopathology* ; Humans ; Animals

With the rapid development of generative artificial intelligence(GAI)technologies,their widespread application in academic research and writing is continuously expanding the boundaries of sci-entific inquiry.However,this trend has also raised a series of ethical and regulatory challenges,inclu-ding issues related to authorship,content authenticity,citation accuracy,and accountability.In light of the growing involvement of AI in generating academic content,establishing an open,controllable,and trustworthy ethical governance framework has become a key task for safeguarding research integrity and maintaining trust within the academic community.This expert consensus outlines ethical requirements across key stages of AI-assisted academic writing-including topic selection,data management,citation practices,and authorship attribution.It aims to clarify the boundaries and ethical obligations surrounding AI use in academic writing,ensuring that technological tools enhance efficiency without compromising in-tegrity.The goal is to provide guidance and institutional support for building a responsible and sustainable research ecosystem.

Objective To explore the correlation between gait disorders and alterations in diffusion tensor imaging(DTI)parameters in different regions of interest(ROI)and associated white matter tracts in older patients with leukoaraiosis(LA).Methods A total of 108 elderly LA patients admitted in our department from July 2023 to May 2024 were enrolled,and based on the Fazekas scale,they were divided into normal(n=28),moderate(n=48),and severe groups(n=32).Tinetti Gait Scale(TGS)was used to evaluate the morbility and balance function,and Short Physical Performance Battery(SPPB)was employed to assess motor function.All participants underwent brain MRI and DTI scanning.Fractional anisotropy(FA)values were quantitatively measured in predefined ROIs and specific white matter tracts.Results The moderate and severe groups had significantly advanced age and higher homocysteine level,and lower total TGS score,Tinetti gait score,total SPPB score,and walking speed score than the normal group(P＜0.05).The severe group exhibited obviously lower education level,and decreased Tinetti balance score and standing score than the normal and moderate groups(P＜0.05).The FA values at the left pa-rietal lobe,the anterior horn of bilateral lateral ventricles,and the splenium of the left corpus cal-losum were notably lower,so were the FA values at the corpus callosum(left-right),left cortico-spinal tract(inferior segment),left anterior thalamic radiation(inferior segment),left inferior fronto-occipital fasciculus(inferior segment),and left superior longitudinal fasciculus(inferior segment)in the moderate group and the severe group than the normal group(P＜0.05).The se-vere group demonstrated lower FA values at the left and right parietal lobes than the other two groups(P＜0.05).Multivariate logistic regression analysis revealed that the anterior horn of the left lateral ventricle(β=-0.683,P=0.001)and the left inferior corticospinal tract(β=-0.742,P=0.001)were influencing factors for gait disorder in elderly LA patients.Conclusion In elderly LA patients,gait disorders are correlated with decreased FA values in specific ROI and white mat-ter tracts,with the decreased FA values in the anterior horn of the left lateral ventricle and the left inferior corticospinal tract demonstrating the most significant impact.

Aim To establish the pyroptosis model of rat chondrocytes induced by tumor necrosis factor α(TNF-α)in order to study the effect of lysine acetyl-transferase 7(KAT7)on pyroptosis of chondrocytes.Methods Chondrocytes of rat knee joint were isolated by type Ⅱ collagenase digestion,and were identified by toluidine blue staining and Col Ⅱ immunofluorescence.CCK-8 was used to evaluate cell viability.Western blot was used to detect the expression of pyroptosis-related proteins NLRP3,GSDMD,caspase-8 and KAT7 in cells intervened with TNF-α,adenovirus overexpression of KAT7(KAT7-oe)and KAT7 inhibitor WM-3835.The microstructure of the cells was observed by scanning e-lectron microscopy.Pyroptosis was detected by TUNEL staining,and the expression of pyroptosis-related pro-tein and KAT7 was detected by immunofluorescence.Results Compared with the empty virus group,KAT7-oe inhibited cell viability,promoted the expression of pyroptosis-related proteins,and TNF-α enhanced this effect.At the same time,the expression of KAT7 and pyroptosis-related proteins in the TNF-α stimulation group increased,and WM-3835 reduced the related proteins expression.Electron microscopy showed that KAT7-oe caused cell swelling,deformation,membrane perforation and rupture,while WM-3835 could restore cell morphology.TUNEL staining and immunofluores-cence results also confirmed that KAT7-oe induced chondrocyte pyroptosis,and WM-3835 could down-reg-ulate the fluorescence of pyroptosis-related proteins.Conclusions The expression of KAT7 increases in rat chondrocyte pyroptosis model,and the intervention of KAT7 expression affects signal molecules related to py-roptosis pathway,suggesting that KAT7 may be related to chondrocyte pyroptosis.

Aim To explore the action mechanism of isoquercitrin(IQ)in ameliorating anxiety based on network pharmacology,cellular transcriptomics,molecu-lar docking and animal experiments.Methods The common targets of anxiety disorders and IQ were ob-tained by using relevant databases.The protein-protein interaction network,the biological function and signa-ling pathway enrichment analysis were conducted by u-sing the common targets.Primary hippocampal neurons were cultured in vitro,and corticosterone was added to induce neurons to establish a corticosterone injury mod-el.IQ treatment was added to the culture system,and transcriptomics was used to screen for differentially ex-pressed genes and enrich for differentially expressed pathways.Subsequently,the results were validated by quantitative real-time polymerase chain reaction(qRT-PCR).Possible targets and signaling pathways for IQ treatment on anxiety were speculated and screened u-sing network pharmacology,transcriptomics and molec-ular docking.The anxiety rat model was constructed,and the anxiety state of rats was evaluated after IQ in-tervention,and the protein expression level of hippo-campus was detected to verify the relevant mechanism.Results Network pharmacology,cellular transcrip-tome,and molecular docking analyses revealed that the key mechanism of IQ for anxiety may be related to the BDKRB2/PI3K/Akt signaling pathway.Animal exper-iments showed that IQ was effective in improving anxie-ty behaviour and learning memory ability in rats.IQ increased the movement distance and residence time in the central area of the open field,the time and number percentage of entries into the open arm in the elevated plus maze,and the spontaneous alternations score in the Y maze in rats,and significantly elevated protein expression of BDKRB2,PI3K,Akt and decreased pro-tein expression of NF-κB in the hippocampus.Conclu-sions Isoquercitrin can effectively treat anxiety,and the mechanism of action may be related to the regula-tion of BDKRB2/PI3K/Akt signaling pathway in hip-pocampus.

Objective To explore the correlation between gait disorders and alterations in diffusion tensor imaging(DTI)parameters in different regions of interest(ROI)and associated white matter tracts in older patients with leukoaraiosis(LA).Methods A total of 108 elderly LA patients admitted in our department from July 2023 to May 2024 were enrolled,and based on the Fazekas scale,they were divided into normal(n=28),moderate(n=48),and severe groups(n=32).Tinetti Gait Scale(TGS)was used to evaluate the morbility and balance function,and Short Physical Performance Battery(SPPB)was employed to assess motor function.All participants underwent brain MRI and DTI scanning.Fractional anisotropy(FA)values were quantitatively measured in predefined ROIs and specific white matter tracts.Results The moderate and severe groups had significantly advanced age and higher homocysteine level,and lower total TGS score,Tinetti gait score,total SPPB score,and walking speed score than the normal group(P＜0.05).The severe group exhibited obviously lower education level,and decreased Tinetti balance score and standing score than the normal and moderate groups(P＜0.05).The FA values at the left pa-rietal lobe,the anterior horn of bilateral lateral ventricles,and the splenium of the left corpus cal-losum were notably lower,so were the FA values at the corpus callosum(left-right),left cortico-spinal tract(inferior segment),left anterior thalamic radiation(inferior segment),left inferior fronto-occipital fasciculus(inferior segment),and left superior longitudinal fasciculus(inferior segment)in the moderate group and the severe group than the normal group(P＜0.05).The se-vere group demonstrated lower FA values at the left and right parietal lobes than the other two groups(P＜0.05).Multivariate logistic regression analysis revealed that the anterior horn of the left lateral ventricle(β=-0.683,P=0.001)and the left inferior corticospinal tract(β=-0.742,P=0.001)were influencing factors for gait disorder in elderly LA patients.Conclusion In elderly LA patients,gait disorders are correlated with decreased FA values in specific ROI and white mat-ter tracts,with the decreased FA values in the anterior horn of the left lateral ventricle and the left inferior corticospinal tract demonstrating the most significant impact.

Objective To explore the influencing factors of different types of small intestinal bacterial overgrowth(SIBO).Methods A total of 539 patients who were hospitalized in the Department of Gastroenterology,the Sixth Medical Center of PLA General Hospital from June 2021 to December 2021 and who underwent methane-hydrogen breath test were retrospectively selected.Based on breath test results,patients were divided into SIBO-negative group(n=300)and SIBO-positive group(n=239).The clinical data were compared between two groups.According to the specific values of breath test results,SIBO-positive patients were further divided into hydrogen-producing bacterial overgrowth(hydrogen-positive,n=103),intestinal methanogen overgrowth(methanogen-positive,n=80),and simultaneous methanogen and hydrogen-producing bacterial overgrowth(double positive,n=56)groups.Multivariate logistic regression analysis was employed to identify influencing factors of different SIBO types.Additionally,SIBO-positive patients were categorized by age into<45 years(n=23),45-60 years(n=82),60-75 years(n=124),and≥75 years(n=10)to compare SIBO positivity rates across age groups.Results The patients in SIBO-positive and double positive groups were older and had a lower body mass index(BMI)than those in SIBO-negative group,with statistically significant differences(P<0.05).Compared with the patients in SIBO-negative group,those in hydrogen-positive group showed a higher proportion of history of coronary heart disease,those in methanogen-positive group were older,and higher proportion of statin use,with statistically significant differences(P<0.05).Multivariate logistic regression analysis revealed that,among different SIBO types,a history of coronary heart disease served as an independent risk factor for hydrogen-producing bacterial overgrowth(OR=2.728,95%CI 1.271-5.857,P=0.010).For methanogen overgrowth,increasing age was identified as an independent risk factor(OR=1.040,95%CI 1.009-1.063,P=0.010),while the application of statin played the role of an independent protective factor(OR=0.420,95%CI 0.236-0.754,P=0.003).As for the simultaneous overgrowth of methane-producing and hydrogen-producing bacteria,increased BMI was found to be an independent protective factor(OR=0.870,95%CI 0.786-0.964,P=0.008).In SIBO-positive group,it was found that for patients aged<45 years,both the methane-positive rate and the double-positive rate were significantly lower than the hydrogen positivity rate(P<0.05).Moreover,among patients aged 45-60 years,the double-positive rate was significantly lower than the hydrogen positivity rate(P<0.01).When it comes to the hydrogen-positive rate,it was significantly lower for patients aged 45-60 and 60-75 years compared with that of patients aged<45 years(P<0.05).In contrast,the methane-positive rate and the double-positive rate were significantly higher for patients aged 45-60 and 60-75 years than those of patients aged<45 years(P<0.01).Conclusion A history of coronary heart disease and increasing age are independent risk factors for intestinal hydrogen-producing bacterial overgrowth and methanogen overgrowth,respectively.The application of statins and increased BMI are independent protective factors for intestinal methanogen simultaneous overgrowth of methanogen and hydrogen-producing bacteria,respectively.