Objective To explore the application effect of bicyclol combined with ganciclovir in the treatment of children with Epstein-barr virus related infectious mononucleosis(IM)based on liver function,immunoglobulin A(IgA),immunoglobulin M(IgM)and immunoglobulin G(IgG)levels.Methods A total of 150 children with EB virus infected and IM admitted to the People's Hospital of Susong County from January 2019 to January 2024 were selected as the study subjects.Random grouping was performed using a digital expression method,dividing them into a control group and an observation group(n=75).All children received routine symptomatic treatment upon admission.The control group were treated with ganciclovir,while the observation group were treated with bicyclol in addition to ganciclovir.Both groups underwent treatment for 7 days.The therapeutic effects,liver function indexes,immunoglobulin indexes,quality of life,and adverse reaction rates were compared between the two groups.The therapeutic effects,liver function indicators,immunoglobulin indicators,quality of life,and adverse reaction rates were compared between the two groups.Results The total effective rate of treatment in the observation group was 93.33％(70/75),which was significantly higher than that of control group(61.33％(46/75)(P＜0.05).After treatment,the liver function indexes of both groups were significantly decreased,and the observation group showing lower levels(all P＜0.05).After treatment,immunoglobulin indexes were significantly increased in both groups,with the observation group having higher levels(P＜0.05).After treatment,the scores of life quality in both groups significantly increased,and those in observation group were higher(all P＜0.05).Quality of life scores significantly improved in both groups,with the observation group achieving higher scores(P＜0.05).The total adverse reaction rate in the observation group was 6.67％(5/75),compared to 5.33％(4/75)in the control group,but there was no significant difference between the two groups(P＞0.05).Conclusion The application of bicyclol combined with ganciclovir in the treatment of Epstein-barr virus related IM can significantly improve the therapeutic effect,improve liver function and immunoglobulin indexes,and improve the quality of life in children,with relatively low adverse reactions,demonstrating high clinical efficacy and safety.