Objective To explore the casual relationship between immune cells and chronic pancreatitis(CP)using Mendelian randomization(MR)analysis.Methods The immune cell phenotypes and CP GWAS data used in this study were obtained from public databases,and 731 immune cell phenotypes were included.The bidirectional MR analysis was used to explore the causal relationship between immune cells and CP,and various sensitivity analysis methods were used to verify the heterogeneity and level multiplicity of the results.Results This study identified 33 immune cell phenotypes with a causal relationship with CP,of which 18 were inhibitory factors,and the rest were risk factors.Among the 18 inhibitory factors,CD25 on CD4+in the Treg cell group showed the most significant inhibitory effect.Among the 15 risk factors,CD8br AC in the TBNK cell group,CD8br on TD CD8br in the mature T cell group,and CD39+CD8br％ T cell and CD28 on CD4+in the Treg cell group showed statistical significance.The reverse MR results further confirmed the unidirectionality of the causal relationship.Conclusion Our study revealed the close relationship between immune cells and CP through MR method,highlighting the complex interaction pattern between the immune system and CP.

Objective:To explore the effects of happy PERMA intervention on resilience, stigma and quality of life in colostomy patients with colorectal cancer.Methods:According to convenience sampling method, 120 patients with colorectal cancer who underwent permanent colostomy in the hospital were enrolled from September 2021 to August 2023. According to admission order, they were divided into control group (60 cases, September 2021-August 2022) and study group (60 cases, September 2022-August 2023). The control group was given routine nursing, while the study group was additionally given happy PERMA mode nursing intervention for 8 weeks. The scores of Connor-Davidson resilience scale (CD-RISC), social impact scale (SIS), ostomy adjustment inventory-20 (OAI-20), quality of life questionnaire core 30(QLQ-C30), general well-being schedule(GWB) and self-care ability before and after 8 weeks of intervention were compared between the two groups using SPSS 22.0 software.Results:After the intervention, the scores of resilience (45.28±2.59), strength (26.34±2.47), optimism (11.05±0.78), and the total CD-RISC score (80.95±3.47) in the study group were significantly higher than those in the control group (37.46±2.08, 18.55±1.96, 8.96±0.63, 65.11±2.32) ( t=18.235, 19.137, 16.146, 29.394, all P<0.05). The scores of internalized stigma (13.09±1.97), economic discrimination (6.85±0.78), social isolation (14.15±1.98), social exclusion (16.93±2.54), and the total SIS score (50.17±2.35) in the study group were significantly lower than those in the control group (13.97±2.44, 7.36±0.95, 16.94±2.37, 19.55±3.42, 58.69±2.83)( t=2.174, 3.214, 6.998, 4.764, 13.756, all P<0.05). The score of persistent worry (11.95±2.16) in the study group was significantly lower than that in the control group (16.57±1.92) ( t=12.383, P<0.05), while the score of acceptance (15.28±1.16), positive life attitude (17.38±2.31), and the total OAI-20 score (58.64±2.08) in the study group were significantly higher than those in the control group (12.19±0.97, 14.55±1.94, 46.30±1.57) ( t=15.829, 7.267, 36.679, all P<0.05). The scores of role function (86.27±3.15), emotional function (81.25±4.33), social function (79.63±3.16), cognitive function (83.55±3.97), general health (83.15±3.16), and the total QLQ-C30 score (407.13±15.92) in the study group were significantly higher than those in the control group (77.62±4.38, 73.85±4.96, 71.80±3.98, 79.42±4.31, 75.34±3.62, 372.65±11.58)( t=12.419, 8.706, 11.935, 5.459, 12.590, 13.567, all P<0.05). The GWB score (86.95±3.57) and self-care ability score (91.13±3.45) in the study group were significantly higher than those in the control group (73.82±4.06, 87.55±4.68)( t=18.812, 4.769, both P<0.05). Conclusion:Happy PERMA mode intervention can effectively improve resilience, reduce stigma and improve quality of life in colostomy patients with colorectal cancer.

Objective:To evaluate the causal relationship between autism spectrum disorder (ASD) and chronic pancreatitis (CP) using a bidirectional two-sample Mendelian randomization (MR) analysis.Methods:Based on genome-wide association study (GWAS) data, forward and reverse two-sample MR analyses were conducted to examine the causal relationship between ASD and CP. Single-nucleotide polymorphisms (SNPs) reaching genome-wide significance were selected from the GWAS data as candidate instrumental variables, and the Steiger directionality test was used to confirm the causal direction. The inverse-variance weighted (IVW) method was applied as the primary analysis to estimate the causal effect of the exposure on the outcome. MR-Egger regression and weighted median methods were used as supplementary sensitivity analyses to assess the robustness of the results. Pleiotropy and heterogeneity tests were performed to evaluate the reliability of the findings.Results:The forward MR analysis ultimately identified 29 SNPs. The IVW analysis indicated that ASD had a significant positive causal effect on CP risk ( OR=1.197, 95% CI 1.047-1.368, P=0.008), with no evidence of horizontal pleiotropy or significant heterogeneity. In the reverse MR analysis, 17 SNPs were included; the IVW analysis did not detect a significant causal effect of CP on ASD ( OR=0.990, 95% CI 0.935-1.047, P=0.717), also with no evidence of horizontal pleiotropy or significant heterogeneity. Causal effect estimates from MR-Egger regression and weighted median analyses were generally consistent with those of IVW. Conclusions:These findings indicate that, at the level of genetic susceptibility, ASD is a risk factor for CP.

Objective:To explore the effects of happy PERMA intervention on resilience, stigma and quality of life in colostomy patients with colorectal cancer.Methods:According to convenience sampling method, 120 patients with colorectal cancer who underwent permanent colostomy in the hospital were enrolled from September 2021 to August 2023. According to admission order, they were divided into control group (60 cases, September 2021-August 2022) and study group (60 cases, September 2022-August 2023). The control group was given routine nursing, while the study group was additionally given happy PERMA mode nursing intervention for 8 weeks. The scores of Connor-Davidson resilience scale (CD-RISC), social impact scale (SIS), ostomy adjustment inventory-20 (OAI-20), quality of life questionnaire core 30(QLQ-C30), general well-being schedule(GWB) and self-care ability before and after 8 weeks of intervention were compared between the two groups using SPSS 22.0 software.Results:After the intervention, the scores of resilience (45.28±2.59), strength (26.34±2.47), optimism (11.05±0.78), and the total CD-RISC score (80.95±3.47) in the study group were significantly higher than those in the control group (37.46±2.08, 18.55±1.96, 8.96±0.63, 65.11±2.32) ( t=18.235, 19.137, 16.146, 29.394, all P<0.05). The scores of internalized stigma (13.09±1.97), economic discrimination (6.85±0.78), social isolation (14.15±1.98), social exclusion (16.93±2.54), and the total SIS score (50.17±2.35) in the study group were significantly lower than those in the control group (13.97±2.44, 7.36±0.95, 16.94±2.37, 19.55±3.42, 58.69±2.83)( t=2.174, 3.214, 6.998, 4.764, 13.756, all P<0.05). The score of persistent worry (11.95±2.16) in the study group was significantly lower than that in the control group (16.57±1.92) ( t=12.383, P<0.05), while the score of acceptance (15.28±1.16), positive life attitude (17.38±2.31), and the total OAI-20 score (58.64±2.08) in the study group were significantly higher than those in the control group (12.19±0.97, 14.55±1.94, 46.30±1.57) ( t=15.829, 7.267, 36.679, all P<0.05). The scores of role function (86.27±3.15), emotional function (81.25±4.33), social function (79.63±3.16), cognitive function (83.55±3.97), general health (83.15±3.16), and the total QLQ-C30 score (407.13±15.92) in the study group were significantly higher than those in the control group (77.62±4.38, 73.85±4.96, 71.80±3.98, 79.42±4.31, 75.34±3.62, 372.65±11.58)( t=12.419, 8.706, 11.935, 5.459, 12.590, 13.567, all P<0.05). The GWB score (86.95±3.57) and self-care ability score (91.13±3.45) in the study group were significantly higher than those in the control group (73.82±4.06, 87.55±4.68)( t=18.812, 4.769, both P<0.05). Conclusion:Happy PERMA mode intervention can effectively improve resilience, reduce stigma and improve quality of life in colostomy patients with colorectal cancer.

Objective:To evaluate the causal relationship between autism spectrum disorder (ASD) and chronic pancreatitis (CP) using a bidirectional two-sample Mendelian randomization (MR) analysis.Methods:Based on genome-wide association study (GWAS) data, forward and reverse two-sample MR analyses were conducted to examine the causal relationship between ASD and CP. Single-nucleotide polymorphisms (SNPs) reaching genome-wide significance were selected from the GWAS data as candidate instrumental variables, and the Steiger directionality test was used to confirm the causal direction. The inverse-variance weighted (IVW) method was applied as the primary analysis to estimate the causal effect of the exposure on the outcome. MR-Egger regression and weighted median methods were used as supplementary sensitivity analyses to assess the robustness of the results. Pleiotropy and heterogeneity tests were performed to evaluate the reliability of the findings.Results:The forward MR analysis ultimately identified 29 SNPs. The IVW analysis indicated that ASD had a significant positive causal effect on CP risk ( OR=1.197, 95% CI 1.047-1.368, P=0.008), with no evidence of horizontal pleiotropy or significant heterogeneity. In the reverse MR analysis, 17 SNPs were included; the IVW analysis did not detect a significant causal effect of CP on ASD ( OR=0.990, 95% CI 0.935-1.047, P=0.717), also with no evidence of horizontal pleiotropy or significant heterogeneity. Causal effect estimates from MR-Egger regression and weighted median analyses were generally consistent with those of IVW. Conclusions:These findings indicate that, at the level of genetic susceptibility, ASD is a risk factor for CP.

Objective To explore the casual relationship between immune cells and chronic pancreatitis(CP)using Mendelian randomization(MR)analysis.Methods The immune cell phenotypes and CP GWAS data used in this study were obtained from public databases,and 731 immune cell phenotypes were included.The bidirectional MR analysis was used to explore the causal relationship between immune cells and CP,and various sensitivity analysis methods were used to verify the heterogeneity and level multiplicity of the results.Results This study identified 33 immune cell phenotypes with a causal relationship with CP,of which 18 were inhibitory factors,and the rest were risk factors.Among the 18 inhibitory factors,CD25 on CD4+in the Treg cell group showed the most significant inhibitory effect.Among the 15 risk factors,CD8br AC in the TBNK cell group,CD8br on TD CD8br in the mature T cell group,and CD39+CD8br％ T cell and CD28 on CD4+in the Treg cell group showed statistical significance.The reverse MR results further confirmed the unidirectionality of the causal relationship.Conclusion Our study revealed the close relationship between immune cells and CP through MR method,highlighting the complex interaction pattern between the immune system and CP.

【Objective】 To explore the causal relationship between education level and pancreatitis risk through Mendelian randomization. 【Methods】 A two-sample Mendelian randomization analysis was conducted using genome-wide association study (GWAS) summary data. The GWAS data for education level and pancreatitis were obtained from SSGAC database and the FinnGen database (version R9). Causal relationship between education level and pancreatitis was explored using the inverse variance weighted (IVW), MR-Egger, and weighted median methods. Heterogeneity and directional pleiotropy were evaluated using Cochran’s Q test and funnel plots. 【Results】 Totally 604 SNPs associated with education level were included. The results provided evidence that there was negative relationship between education level and pancreatitis risk. For acute pancreatitis, OR=0.52, 95% CI: 0.44-0.62, P=2.43×10^-14 while for chronic pancreatitis, OR=0.51, 95% CI: 0.41-0.64, P=7.20×10^-9. Results from MR-Egger and weighted median analyses obtained the same results. The results of sensitivity analysis indicated that this study did not violate the basic assumptions of Mendelian randomization. 【Conclusion】 There is a causal relationship between education level and the occurrence of pancreatitis. The educational level is negatively correlated with the risk of pancreatitis.

This paper summarized the clinical experience of Professor LIU Shenlin in diagnosing and treating gastric cancer with the thinking of "prescription according to tendency". In this paper， the thinking of diagnosis and treatment for gastric cancer and medication skills were summarized into four dimensions： power， energy， chronology and situation. The diagnosis emphasizes the tendency of qi power， considering that the onset of gastric cancer primarily stems from the disorder of qi movement in zang-fu organs， and emphasizing the importance of regulating the liver and spleen qi to intercept the tendency of disease. It points out that dampness-phlegm-stasis-toxin is a crucial link leading to the metabolic imbalance of body energy， and Professor LIU adepts at using methods such as breaking up blood and expelling stasis， and clearing heat and resolving toxins to block the pathological chain reaction caused by energy imbalance and to restore the homeostasis of the organism. In the treatment process， according to the characteristics of gastric cancer staging and chronological evolution， we will explore the changes of the exuberance and weakness of healthy qi and pathogenic qi in the context of the chronologic tendency， and adjust the dosage of attacking and tonic medicines in different stages of the disease in order to balance and restore the body. The "situation" is in line with the state of consumptive disease in advanced gastric cancer， Professor LIU skillfully uses large doses of Huangqi （Astragalus mongholicus） with flexible combination of medicinals to replenish deficiencies， invigorate qi， and regulate blood vessels.

Pancreatic cancer has a very poor prognosis.Early diagnosis and treatment are especially critical for improving its prognosis.Nanotechnology has been widely used in the diagnosis and treatment of pancreatic cancer.Relying on the unique physicochemical properties of nanoparticles and their rich surface modifications,effective enrichment of tumor sites can be achieved.Magnetic iron oxide nanoparticles(MIONPs)is one of the commonly used nanomaterials in the diagnosis and treatment of pancreatic cancer,and has good biocompatibility.Through special surface modification,it can be used in targeted diagnosis and treatment of pancreatic cancer.MIONPs can be used as a contrast agent for MRI,and by modifying the surface,they also can be used in targeted imaging of pancreatic cancer.And they can also be modified as a drug delivery system to achieve targeted delivery of drugs and improve therapeutic effects.However,the application of MIONPs in pancreatic cancer diagnosis and treatment still faces some challenges,such as nanotoxicity and cost issues.With the development of technology,MIONPs are expected to play an important role in the personalized diagnosis and treatment of pancreatic cancer.

Objective:To investigate the role of RASAL2 in vasculogenic mimicry in pancreatic cancer cells and to preliminarily explore the potential molecular mechanisms involved.Methods:The clinical proteomic tumor analysis consortium(CPTAC)database was used to analyze the expression of RASAL2 in pancreatic cancer,and immunohistochemical method was used to detect the expression of RASAL2,β-catenin and Vimentin in pancreatic cancer and adjacent tissues.In the pancreatic cancer cell line,after lentivirus infection knockdown and overexpression of RASAL2,transcriptome sequencing was performed on pancreatic cancer cells silencing RASAL2 expression,and vasculogenic mimicry experiment was used to detect the effect of RASAL2 on angiogenesis of pancreatic cancer cells;The molecular mechanism of RASAL2 promoting vasculogenic mimicry in pancreatic cancer was studied by real-time fluorescent quantitative PCR and Western blotting detection.Results:The analysis results of CPTAC database showed that the expression level of RASAL2 protein in pancreatic cancer tissue was significantly higher than that in normal pancreatic tissue.Immunohistochemical test results showed that the expression of RASAL2 in pancreatic cancer tissue was up-regulated,and the expression of β-catenin and Vimentin in pancreatic cancer tissue was also significantly up-regulated.The second-generation transcriptome sequencing results showed that RASAL2 may be involved in the biological behavior of intercellular connections and adhesion.After knocking down RASAL2 expression in pancreatic cancer cell PANC-1,the expression level of AKT/β-catenin signaling pathway related proteins and vasculogenic mimicry related proteins decreased,and the formation of vasculogenic mimicry structure was inhibited;After overexpression of RASAL2 in pancreatic cancer cell BxPC-3,the expression level of AKT/β-catenin signaling pathway related proteins and vasculogenic mimicry related proteins increased,promoting the formation of vasculogenic mimicry.Conclusion:The effect of RASAL2 on vasculogenic mimicry in pancreatic cancer cells is related to AKT/β-catenin signaling pathway.