Objective: To investigate the associations of screen time (ST) and dietary behavior with sleep quality among first-year junior high school students, and further analyze the joint exposure effect of weekend ST and dietary usage, so as to provide scientific evidence for improving adolescent sleep quality and developing related health intervention measures. Methods: From September 19 to October 18, 2023, a combination of cluster sampling and convenience sampling method was used to select 733 first-year junior high school students from 4 public junior high schools in Jinshan District, Shanghai. ST on weekdays and weekends of students was investigated. The Chinese Diet Quality Questionnaire and the Pittsburgh Sleep Quality Index were applied to assess dietary behaviors and sleep quality. Binary Logistic regression was used to analyze the associations of ST and dietary behavior with sleep quality during weekdays and weekends. Results: The proportion of students with high level ST was 31.8% on weekdays and 65.3% on weekends. Students with poor dietary quality accounted for 45.3%, and the detection rate of poor sleep quality was 14.6 %. Binary Logistic regression analysis showed that, compared with students with low level ST on weekend and good dietary quality, those with high level ST on weekend and those with poor dietary quality had increased risks of poor sleep quality [ OR (95% CI )=1.89(1.10-3.26), 2.30(1.47-3.58), both P <0.05]. Combined exposure showed that compared to students with good diet quality and low level ST on weekend, students with good dietary quality and high level ST on weekend, those with poor dietary quality and low level ST on weekend, and those with poor dietary quality and high level ST on weekend also had increased risks of poor sleep quality [ OR (95% CI )=3.00(1.25-7.25), 4.07(1.60-10.33), 5.72(2.37-13.81), all P <0.05]. Conclusion Excessive weekend ST and poor dietary quality are associated with poor sleep quality among first-year junior high school students, and their co-occurrence further increases the risk.

Objective: To investigate the association of moderate to vigorous intensity physical activity (MVPA) and screen time (ST) with emotional and behavioral problems in middle school students, so as to provide a basis for improving the mental health level of adolescents. Methods: Four public junior high schools in Jinshan District, Shanghai were selected by random cluster method from September 19 to October 18, 2023, with 779 first grade students included. Strengths and Difficulties Questionnaire (SDQ), as well as physical activity and screen time were collected. Binary Logistic regression analysis was used to investigate the association between MVPA, ST and different dimensions of emotional and behavioral problems. Results: Students with MVPA<60 min/d accounted for 82.4%, and with ST>2 h/d accounted for 29.5%. The abnormal rate of SDQ dimensions among students ranged from 6.8 % to 19.3%, with the highest abnormal rate observed in behavioral problems. The results of binary Logistic regression analysis showed that, compared with high level of MVPA, students with low level of MVPA had a significantly increased risk of developing problems in prosocial behavior [ OR (95% CI )=2.16(1.37-3.41), P <0.05]. Compared with low level ST, students with high level had an increased risk of experiencing emotional symptoms, behavioral problems and peer interaction problems [ OR (95% CI )=2.00(1.25-3.22), 2.00(1.41-2.84), and 1.69(1.22-2.33), P <0.05]. Compared with high MVPA and low ST, students with low MVPA and high ST had an increased risk of developing problems in the dimensions of conduct problems, prosocial behavior, and overall difficulty score [ OR (95% CI )=1.92(1.13-3.23), 2.73(1.56-4.77), and 2.07(1.19-3.60), P <0.05]. Conclusions Physical activity and screen time are related to emotional and behavioral problems in middle school students. Insufficient physical activity time and excessive screen time might increase the risk of emotional and behavioral problems. Interventions can be administered on physical activity and screen time among adolescents to promote their mental health.

Diffuse large B-cell lymphoma (DLBCL) is an aggressive type of non-Hodgkin's lymphoma. A total of 10%‒15% of DLBCL cases are associated with myelocytomatosis viral oncogene homolog(MYC) and/or B-cell lymphoma-2 (BCL2) translocation or amplification. BCL2 inhibitors have potent anti-tumor effects in DLBCL; however, resistance can be acquired through up-regulation of alternative anti-apoptotic proteins. The histone deacetylase (HDAC) inhibitor chidamide can induce BIM expression, leading to apoptosis of lymphoma cells with good efficacy in refractory recurrent DLBCL. In this study, the synergistic mechanism of chidamide and venetoclax in DLBCL was determined through in vitro and in vivo models. We found that combination therapy significantly reduced the protein levels of MYC, TP53, and BCL2 in activated apoptotic-related pathways in DLBCL cells by increasing BIM levels and inducing cell apoptosis. Moreover, combination therapy regulated expression of multiple transcriptomes in DLBCL cells, involving apoptosis, cell cycle, phosphorylation, and other biological processes, and significantly inhibited tumor growth in DLBCL-bearing xenograft mice. Taken together, these findings verify the in vivo therapeutic potential of chidamide and venetoclax combination therapy in DLBCL, warranting pre-clinical trials for patients with DLBCL.
Aminopyridines ; Animals ; Benzamides ; Biological Phenomena ; Bridged Bicyclo Compounds, Heterocyclic ; Down-Regulation ; Histone Deacetylase Inhibitors/therapeutic use* ; Humans ; Lymphoma, Large B-Cell, Diffuse/pathology* ; Mice ; Neoplasm Recurrence, Local ; Proto-Oncogene Proteins c-bcl-2/metabolism* ; Proto-Oncogene Proteins c-myc/therapeutic use* ; Sulfonamides ; Tumor Suppressor Protein p53/metabolism*