Objective : To investigate whole genome information of a newly isolatedBifidobacterium animalissubsp. lactic B4 strain from healthy human feces was analyzed and its probiotic properties. Methods : The antimicrobial resistance, hemolytic, gastric acid tolerance and biochemical characteristics of B. animalis B4 were evaluated byin vitroexperiments, and its whole genome was sequentially sequenced and functional annotation was performed by next and three-generation sequencing technology. Results: Whole genome sequencing of B. animalis B4 showed that its genome size was 1 944 146 bp, with GC content of 60.49%, no plasmid, and a total of 1 642 genes. The results ofin vitroanalysis showed that the B. animalis B4 had good probiotic properties, including non-hemolytic and stomach acid resistance. At the same time, the genome results showed that the B. animalis B4 strain did not have toxin and disease-related genes, drug resistance genes were few and the transmission ability was not high, so it had high safety. Gene annotation of KEGG, COG and GO showed that it contained many biological active enzymes, such as β-galactosidase, L-lactate dehydrogenase and other probiotic genes. Conclusion The B. animalis B4 has good probiotic properties, showing excellent safety at the genetic level, with a probiotic gene sequence.

Objective To analyze the relationship between serum solute carrier family 7 member 11(SLC7A11)and glutathione peroxidase 4(GPX4)levels and chemotherapy sensitivity and prognosis in pros-tate cancer patients.Methods A total of 106 prostate cancer patients admitted to the hospital from January 2019 to December 2022 were selected as the prostate cancer group,106 healthy males who came to the hospital for physical examination were selected as the control group.Enzyme linked immunosorbent assay(ELISA)was applied to detect the levels of SLC7A11 and GPX4 in serum.The prognostic factors of prostate cancer pa-tients were analyzed by multivariate Logistic regression.The predictive value of SLC7A11 and GPX4 levels in serum for the prognosis of prostate cancer patients were analyzed by receiver operating characteristic(ROC)curve.Results Compared with the control group,the serum levels of SLC7A11 and GPX4 in the prostate cancer group were obviously increased(P＜0.05).Compared with before chemotherapy,the serum levels of SLC7A11 and GPX4 in the prostate cancer patients after chemotherapy were obviously reduced(P＜0.05).The serum level of SLC7A11 in the prostate cancer patients with different tumor maximum diameter,Gleason score,clinical pathological stage,lymph node metastasis,tumor differentiation degree,and prostate-specific an-tigen(PSA)level were statistically significant(P＜0.05).The serum level of GPX4 in the prostate cancer pa-tients with different tumor differentiation degrees,Gleason scores,clinical pathological stages,lymph node me-tastasis,and PSA level were statistically significant(P＜0.05).The serum levels of SLC7A11 and GPX4 in the poor prognosis group were obviously higher than those in the good prognosis group(P＜0.05).Multivariate Logistic regression analysis results showed that the levels of SLC7A11 and GPX4 were influencing factors for the prognosis of prostate cancer(P＜0.05).The results of ROC curve analysis showed that the combination of SLC7A11 and GPX4 levels in serum had a better prognostic value than single index(P＜0.05).Conclusion The levels of SLC7A11 and GPX4 in the serum of prostate cancer patients are obviously in-creased,which is closely related to chemotherapy sensitivity and could be used as biomarkers for predicting the prognosis of prostate cancer patients.

Objective To explore the nursing essentials of complicated incision infection and embedding syndrome after percutaneous endoscopy gastrojejunostomy(PEGJ). Methods Comprehensive treatment and care including improved nursing methods, negative pressure suction, the dressing strengthening, and enteral nutrition. Results The swelling of the incision gradually subsided. On the 18th day, there was slight redness around the tube, no exudate, no fever, and the incision healed well. On the 20th day, family members were taught with routine maintenance method of the PEG/J tube, and the patient was discharged from hospital with the tube home for continue treatment. Conclusion Comprehensive nursing methods according to cause analysis can improve the curative effect of complications after PEGJ implantation.

The expensive production of bioethanol is because it has not yet reached the 'THREE-HIGH' (High-titer, high-conversion and high-productivity) technical levels of starchy ethanol production. To cope with it, it is necessary to implement a high-gravity mash bioethanol production (HMBP), in which sugar hydrolysates are thick and fermentation-inhibitive compounds are negligible. In this work, HMBP from an atmospheric glycerol autocatalytic organosolv pretreated wheat straw was carried out with different fermentation strategies. Under an optimized condition (15% substrate concentration, 10 g/L (NH4)2SO4, 30 FPU/g dry matter, 10% (V/V) inoculum ratio), HMBP was at 31.2 g/L with a shaking simultaneous saccharification and fermentation (SSF) at 37 degrees C for 72 h, and achieved with a conversion of 73% and a productivity of 0.43 g/(L x h). Further by a semi-SFF with pre-hydrolysis time of 24 h, HMBP reached 33.7 g/L, the conversion and productivity of which was 79% and 0.47 g/(L x h), respectively. During the SSF and semi-SSF, more than 90% of the cellulose in both substrates were hydrolyzed into fermentable sugars. Finally, a fed-batch semi-SFF was developed with an initial substrate concentration of 15%, in which dried substrate (= the weight of the initial substrate) was divided into three portions and added into the conical flask once each 8 h during the first 24 h. HMBP achieved at 51.2 g/L for 72 h with a high productivity of 0.71 g/(L x h) while a low cellulose conversion of 62%. Interestingly, the fermentation inhibitive compound was mainly acetic acid, less than 3.0 g/L, and there were no other inhibitors detected, commonly furfural and hydroxymethyl furfural existing in the slurry. The data indicate that the lignocellulosic substrate subjected to the atmospheric glycerol autocatalytic organosolv pretreatment is very applicable for HMBP. The fed-batch semi-SFF is effective and desirable to realize an HMBP.
Biofuels ; Carbohydrates ; chemistry ; Cellulose ; chemistry ; Ethanol ; metabolism ; Fermentation ; Furaldehyde ; chemistry ; Glycerol ; chemistry ; Hydrolysis ; Triticum